• 생명과학회지
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• 제25권8호
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• pp.903-909
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• 2015

LAMP (Loop-mediated Isothermal Amplification)법은 PCR를 기반으로 등온에서 autocycling 가닥 변위 DNA 합성에 의존하며, Bst polymerase를 사용하여 진단하는 방법이다. 이것은 대상 DNA의 여섯 개의 배열을 인식하는 4개의 특정 primer의 도움을 받아 단시간 안에 병원체를 식별하는 높은 특이성을 지니고 있다. 본 연구에서는 LAMP로 수생에서 위험한 병원체인 Vibrio alginolyticus의 특별한 LAMP primer를 제작하였으며, 신속한 진단을 위해 MgSO₄, dNTP, Betaine, Bst polymerase의 최적 반응 조건의 특이성 및 기존의 PCR보다 10배 정도의 민감하다는 것을 확인하였다. 또한, 디자인 되어진 LAMP primer가 다른 Vibrio 종들 중 오직 V. alginolyticus에서만 반응한 것을 확인 할 수 있었다. 본 논문에서는 병원체 세균인 V. alginolyticus의 빠르고 민감한 효과적인 진단으로 양식 질병들을 조기에 발견할 수 있도록 개발하였다.

• Journal of Power Electronics
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• 제16권4호
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• pp.1551-1564
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• 2016

The high penetration level of inverter-based distributed generation (DG) power plants is challenging the low-voltage ride-through requirements, especially under unbalanced voltage sags. Recently, a flexible injection of both positive- (PS) and negative-sequence (NS) reactive currents has been suggested for the next generation of grid codes. This can enhance the ancillary services for voltage support at the point of common coupling (PCC). In light of this, considering distant grid faults that occur in a mainly inductive grid, this paper proposes a complete voltage support control scheme for the interface inverters of medium or high-rated DG power plants. The first contribution is the development of a reactive current reference generator combining PS and NS, with a feature to increase the PS voltage and simultaneously decrease the NS voltage, to mitigate voltage imbalance. The second contribution is the design of a voltage support control loop with two flexible PCC voltage set points, which can ensure continuous operation within the limits required in grid codes. In addition, a current saturation strategy is also considered for deep voltage sags to avoid overcurrent protection. Finally, simulation and experimental results are presented to validate the effectiveness of the proposed control scheme.

• Journal of Power Electronics
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• 제18권4호
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• pp.1245-1254
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• 2018

The stability of a grid-connected system (GCS) has become a critical issue with the increasing utilization of renewable energy sources. Under grid faults, however, a grid-connected inverter cannot work efficiently by using only the traditional droop control. In addition, the unbalance factor of voltage/current at the common coupling point (PCC) may increase significantly. To ensure the stable operation of a GCS under grid faults, the capability to compensate for grid imbalance should be integrated. To solve the aforementioned problem, an improved voltage-type grid-connected control strategy is proposed in this study. A negative sequence conductance compensation loop based on a positive sequence power droop control is added to maintain PCC voltage balance and reduce grid current imbalance, thereby meeting PCC power quality requirements. Moreover, a stable analysis is presented based on the small signal model. Simulation and experimental results verify the aforementioned expectations, and consequently, the effectiveness of the proposed control scheme.

• Journal of Power Electronics
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• 제13권3호
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• pp.469-478
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• 2013

This paper proposes a novel scheme for the current controller for the grid-side converter (GSC) of permanent-magnet synchronous generator (PMSG) wind turbines to eliminate the high-order harmonics in the grid currents under grid voltage disturbances. The voltage unbalance and harmonics in three-phase systems cause grid current distortions. In order to mitigate the input current distortions, multi-loop current controllers are applied, where the positive-sequence component is regulated by proportional-integral (PI) controllers, and the negative-sequence and high-order harmonic components are regulated by proportional-resonance (PR) controllers. For extracting the positive/negative-sequence and harmonic components of the grid voltages and currents without a phase delay or magnitude reduction, composite observers are applied, which give faster and more precise estimation results. In addition, an active damping method using PR controllers to damp the grid current component of the resonant frequency is employed to improve the operating stability of VSCs with inductor-capacitor-inductor (LCL) filters. The validity of the proposed method is verified by simulation and experimental results.

• Journal of Power Electronics
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• 제17권3호
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• pp.716-724
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• 2017

The output voltage of an off-grid inverter is influenced by load current, and the voltage harmonics especially the 5th and 7th are increased with nonlinear loads. In this paper, to attenuate the output voltage harmonics of off-grid inverters with nonlinear loads nearby, a load current feedforward is proposed. It is introduced to a voltage control loop based on the Positive and Negative Sequence Harmonic Regulator (PNSHR) compensation to modify the output impedance at selective frequencies. The parameters of the PNSHR are revised with the output impedance of the off-grid inverter, which minimizes the output impedance of the off-grid inverter. Experimental results verify the proposed method, showing that the output voltage harmonics caused by nonlinear loads can be effectively suppressed.

• 대한기관식도과학회지
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• 제4권1호
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• pp.73-78
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• 1998

The mutation of p53 is the most common genetic alteration found in human cancers and has oncogenic properties. mdm-2 is a recently discoverd that controls the p53 activity by binding of its protein, so negative feedback loop has been suggested in which p53 induces mdm-2 expression. The purpose of this study was to analyze the expression of p53 in leukoplakias, mdm-2 in squamous cell carcinomas, and relationship between p53 and mdm-2 expression in leukoplakias and squamous cell carcinomas. The results were as follows : 1) The p53 was expressed 33.4% in leukoplakias 2) The mdm-2 was expressed 8.3% in leukoplakias and 22.7% in squamous cell carcinomas. 3) The expression rate of p53 was higher in specimens negative for mdm-2 than in specimens positive for mdm-2, but there was not significant relationship between p53 and mdm-2 expression. In conclusion p53 was thought to participate in early phase of oncogenesis, and mdm-2 was thought to have a role as a oncogene in squamous cell carcinoma of head and neck. Though there was not significant relationship between p53 and mdm-2 expression, mdm-2 was thought to inhibit p53 activity.

• Journal of Power Electronics
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• 제19권1호
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• pp.89-98
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• 2019

This paper analyzes the input side performance of a conventional three-phase to single-phase matrix converter (3-1MC). It also presents the input-side waveform quality under this topology. The suppression of low-frequency input current harmonics is studied using the 3-1MC plus capacitance compensation unit. The constraint between the modulation function of the output and compensation sides is analyzed, and the relations among the voltage utilization ratio and the output compensation capacitance, filter capacitors and other system parameters are deduced. For a 3-1MC without large-capacity energy storage, the system performance is susceptible to input voltage imbalance. This paper decouples the inner current of the 3-1MC using a Lyapunov function in the input positive and negative sequence bi-coordinate axes. Meanwhile, the outer loop adopts a voltage-weighted synthesis of the output and compensation sides as a cascade of control objects. Experiments show that this strategy suppresses the low-frequency input current harmonics caused by input voltage imbalance, and ensures that the system maintains good static and dynamic performances under input-unbalanced conditions. At the same time, the parameter selection and debugging methods are simple.

• Structural Engineering and Mechanics
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• 제87권2호
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• pp.117-127
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• 2023

To efficiently attenuate seismic responses of a structure, a novel pneumatic-driven active dynamic vibration absorber (PD-ADVA) is proposed in this study. PD-ADVA aims to realize closed-loop control using a simple and intuitive control algorithm, which takes the structure velocity response as the input signal and then outputs an inverse control force to primary structure. The corresponding active control theory and phase control mechanism of the system are studied by numerical and theoretical methods, the system's control performance and amplitude-frequency characteristics under seismic excitations are explored. The capability of the proposed active control system to cope with frequency-varying random excitation is evaluated by comparing with the optimum tuning TMD. The analysis results show that the control algorithm of PD-ADVA ensures the control force always output to the structure in the opposite direction of the velocity response, indicating that the presented system does not produce a negative effect. The phase difference between the response of uncontrolled and controlled structures is zero, while the phase difference between the control force and the harmonic excitation is π, the theoretical and numerical results demonstrate that PD-ADVA always generates beneficial control effects. The PD-ADVA can effectively mitigate the structural seismic responses, and its control performance is insensitive to amplitude. Compared with the optimum tuning TMD, PD-ADVA has better control performance and higher system stability, and will not have negative effects under seismic wave excitations.

• 한국전자파학회논문지
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• 제28권12호
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• pp.933-940
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• 2017

본 논문에서는 부성 임피던스 변환기(Negative Impedance Converter: NIC)를 적용한 무선전력전송 시스템을 제안하였다. 차폐물질의 영향을 고려하여 전송 시스템을 구성하였다. 전송효율 개선을 위하여 부성 임피던스 변환기에서 발생한 부성저항을 적용하여 송신단의 Q 인자가 향상했다. NIC는 연산증폭기와 저항 소자로 구현하였으며, 특정 저항에 따른 부성저항 특성을 얻었다. 송신 코일의 크기는 $250mm{\times}250mm{\times}0.8mm$이며, 임피던스와 Q 인자는 각각 $31+j1874{\Omega}$, 60이다. 부성저항이 약 $30{\Omega}$일 때, 송신단의 저항이 감소하여 Q 인자는 약 900으로 증가했으며, 이는 기존 대비 약 15배 향상된 결과이다. 제안하는 시스템에 대하여 전송 효율을 측정하였으며, 기존 시스템과 비교하여 효율이 크게 향상되었다. 따라서 NIC의 효과로 전송 효율이 개선될 수 있는 것을 검증하였다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.108-108
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• 2001

Axin2/Conductin/Axil and its ortholog Axin are negative regulators of the Wnt signaling pathway, which promote the phosphorylation and degradation of ${\beta}$-catenin. While Axin is expressed ubiquitously, Axin2 mRNA was seen in a restricted pattern during mouse embryogenesis and organogenesis. Because many sites of Axin2 expression overlapped with those of several Wnt genes, we tested whether Axin2 was induced by Wnt signaling. Endogenous Axin2 mRNA and protein expression could be rapidly induced by activation of the Wnt pathway, and Axin2 reporter constructs, containing a 5.6 kb DNA fragment including the promoter and first intron, were also induced. This genomic region contains eight Tcf/LEF consensus binding sites, five of which are located within longer, highly conserved non-coding sequences. The mutation or deletion of these Tcf/LEF sites greatly diminished induction by ${\beta}$-catenin, and mutation of the Tcf/LEF site T2 abolished protein binding in an electrophoretic mobility-shift assay. These results strongly suggest that Axin2 is a direct target of the Wnt pathway, mediated through Tcf/LEF factors. The 5.6 kb genomic sequence was sufficient to direct the tissue specific expression of d2EGFP in transgenic embryos, consistent with a role for the Tcf/LEF sites and surrounding conserved sequences in the in vivo expression pattern of Axin2. Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal.