• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.720-728
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• 2000

The different products of neem (Azadirachta indica) are utilized for variety of purposes in industry, health and animal agriculture in the Indian subcontinent. The cake from seeds after oil extraction is a good source of nutrients (CP: 35-38%; EE: 4.5-5.5%; CF: 12-15%; Ca: 0.75%; P: 0.45% on DM), and in particular, the one out of its kernel is proteinaceous and is relatively balanced in its amino acid and mineral profile. But the cake is toxic and bitter to taste owing to triterpenoids (nimbin, salannin, azadirachtin), which restricts its safe inclusion in livestock diet. Several feeding trials with raw cake have revealed poor palatability and adverse performance among different categories of livestock and poultry. Internal organ changes included histological alteration in intestine, liver, kidney and distruption of spermatogenesis and ovarian activity. Ruminants appears to tolerate reasonably higher levels of the cake and to a limited low levels of dietary inclusion also proved to be tolerable in monogastric farm animals. Debitterization through solvent (hexane, ether) extraction, water washing, alkali (NaOH, 1.5, 2.5 or 3%, wt/wt) soaking and urea (1.5 or 3%, wt/wt) - ammoniation have been tried with appreciable success in improving the palatability and nutritive value of the cake. For enhanced utilization, decortication of neem seeds is to be done effectively at industrial level with maximum oil recovery. The resultant proteinaceous kernel by-product could be a cheaper unconventional protein supplement after suitable processing.

• Mycobiology
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• v.33 no.4
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• pp.194-199
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• 2005

Application of fertilizers such as urea, diammonium phosphate (DAP) and muriate of potash in soil adversely affected the spore germination of Arthrobotrys dactyloides. Amendment of soil with urea at the concentrations of 1.0%, 0.5% and 0.1 % completely inhibited spore germination and direct trap formation on the conidium, whereas muriate of potash delayed and reduced the spore germination even at the lowest concentration. DAP also inhibited spore germination at 1.0% concentration, while at lower concentration the percentage of spore germination was reduced. Application of neem cake at the concentration of 0.5% also inhibited spore germination after 24 h of amendment. The inhibitory effect of neem cake was reduced after 15 days of amendment, while after 30 days after amendment the inhibitory effect was completely lost and the spore germinated by direct trap as in unamended soil. Nematodes were not attracted to ungerminated spores after 24 h of amendment. After 15 days of amendment nematodes were attracted to agar blocks containing fewer germinated spores after 24 h of incubation but after 48 h of incubation large number of nematodes were attracted and trapped by the germinated spores with direct traps. After 30 days of amendment, larger number of nematodes were attracted and trapped by direct traps.

• Korean Journal of Organic Agriculture
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• v.21 no.3
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• pp.363-372
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• 2013

This experiment was carried out for development of environment-friendly agriculture materials to control Bemisia tabaci (Gennadius) in eggplant. Plant growth investigated the spray at intervals of 7 days after transplanting on 8 environment-friendly agriculture materials, such as egg-yolk and cooking oil, Results were to be good in the control and neem products. B. tabaci population density adult and nymph was low at the dinotefuran WG chemical treatment, and also low neem products of environment-friendly agriculture material. We have investigated the effect of different concentration of neem products on B. tabaci population density. Yield was high in the dinotefuran WG, and hight in the neem products and papper seed oil, but low in the sophora extracts and Ginko net sarcocarp. In the case of neem products treatment indicated 52 percent yield more than control.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.4
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• pp.398-401
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• 1997

A caecal fermentation study was conducted in 30 Angora rabbits equally placed under five whole diets (75 concentrate : 25 roughage) supplemented with processed Neem (Azadirachta indica) kernel meal (NKM ; 2% urea or 1.5% NaOH, W/W), replacing isonitrogenously either at 50 or 100% level of deoiled peanut (Arachis hypogea) meal, for 18 wk period. The total volatile fatty acids level was depressed (p < 0.05) in all the experimental groups (0.02 to 0.04 mEq/g) and ammonia-nitrogen was lowest (p < 0.05; $24.9{\mu}mol/g$) in rabbits fed NaOH treated NKM supplemented diet. Enzyme activity (unit/g) of carboxy methyl-cellulase, ${\alpha}$-amylase, protease and urease exhibited much variation and did not differ significantly. Hence, the results could not confirm a possible adverse effect of feeding NKM on caecal fermentation.

• Archives of Pharmacal Research
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• v.13 no.1
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• pp.108-112
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• 1990

It was confirmed that extracts of some plants were used in folklore medicine as hypoglycemic agents. Of these plants is Azadirachta indica ("neem") which grows in tropical regions. The present study deals with biochemical effects of the "neem" leaf water extract given orally to experimental aminals, especially the hypoglycemic characteristics. Normals as well as alloxan diabetic rats have been used in this work. The results showed that the "neem" leaf extract produced some hypoglycemia in normal rats when given in two doses, while in diabetic rats there was a decrease in blood sugar, but it could not alleviate the diabetic in body weight loss and high percentage mortality, especially with a high dose. It was observed increased with concomitant decrease in the liver fat as compared to normal levels. There was also a drop in liver proteins which was dose-related. The results were compared wiht those obtained with an oral hypoglycemic drug (Glibenclamide).glycemic drug (Glibenclamide).

• Journal of Forest and Environmental Science
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• v.29 no.4
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• pp.282-291
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• 2013

Rapid loss in viability of neem (Azadirachta indica A. Juss.) seed is a major problem. Present effort was undertaken for developing a set pattern for assessing of viability and vigour in seed of various mother tree age of neem (Age I-06 years, Age II-15 years, Age III-25 years and Age IV->30 years old). Various viability test viz. triphenyle tetrazolium chloride test, electrical conductivity, excised embryo test, and germination test have been performed on seeds obtained from mother tree age classes. Inconsistency was observed with the TTC and EC test in germination of seed in laboratory as well as nursery. While various vigour tests viz. cold test, chemical stress test (methanol stress test), and accelerated ageing test alongwith ageing index, germination test (G%, MGT and GV) and various seedling growth parameters like seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices i.e. vigour index, sturdiness quotient, volume index, quality index, root shoot ratio in nursery as well have been taken for study and showed better consistency. On the basis present study results of various viability and vigour test indicated that mother tree age class II performed better in comparison to others and it can be recommended for seed collection. Further it is also recommended that viability of neem seed may be assessed using various laboratory tests like excise embryo test and germination test (G%, MGT and GV) and vigour test may be taken preferably by cold germination test, chemical (methanol) stress test, accelerated ageing test in laboratory and germination alongwith various seedling growth parameters seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices like Vigour Index, Sturdiness quotient, Volume Index, Quality index, root shoot ratio in nursery as discussed in this study.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.528-534
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• 2009

The study of germination and seedling growth characteristics of Neem under different soil and environment conditions was undertaken. The seed germination started 8 days after sowing in commercial bed soil, whereas, delayed germination was observed in sandy-loam (15 days) and sandy (19 days) soil. The highest germination (73.33%) was observed in commercial bed soil in green house, whereas, the lowest germination was observed in sandy soil (16.67%) and sandy-loam soil (8.33%). The seeds in the open field (sandy soil) also showed poor (10%) germination. The mean number of germination seed/day (GD) and seed germination vigor rate (GV) both were highest in the commercial bed soil with 0.733% and 16.67% respectively in the green house, whereas sandy and sandy-loam soil in green house and open field (sandy soil) all showed much lower GD and GV values. The seedling characteristics of nursery revealed that the seedling grown in the growth chamber in commercial bed soil was significantly higher in all the parameters comparing to others grown in green house and open field. The growth was nearly 7 fold in the chamber compared to that of the green house nursery observed in three months old seedlings. Likewise, HPLC analysis revealed that the green house grown seedling contain higher quantity of pigments compare to the chamber grown seedlings. Among the soils used the commercial soil alone or in combination with sandy and sandy-loam soil in the ratio of 2:1:1 respectively with the temperature of $27{\pm}2^{\circ}C$ showed better for Neem nursery preparation.

• Advances in environmental research
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• v.3 no.1
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• pp.1-9
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• 2014

Effect of automobile polluted soil with five soil concentration (0 (Control), 25, 50, 75 and 100%) was observed on early seedling growth performance and biomass production of Neem (Azadirachta indica A. Juss). The treatment of 75% automobile polluted soil significantly (p < 0.05) decreased the seedling length (18.60 cm) of A. indica. The automobile polluted soil treatment with the concentration of 50% slightly increased the root length as compared to control. The automobile polluted soil treatment with the concentration of 25, 50, 75 and 100% negatively affected shoot length of A. indica as compared to control. The treatment of all concentration of automobile polluted soil progressively decreased the total leaf area A. indica as compared to control soil treatment. The automobile polluted soils also showed negative effects on biomass production of A. indica. The automobile polluted soil treatment at 25% concentration significantly (p < 0.05) affected shoot, leaves and seedling dry weight of A. indica as compared to control soil treatment. The order of relationship between production of A. indica's seedling dry weight and automobile polluted soil treatment was observed as root > shoot > leaves > total seedling.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.30-34
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• 2001

The expediency of promoting rabbit production on underutilized by-product feedstuffs prompted the current investigation of caecal fermentation pattern, blood biochemical profile and histopathological changes of vital organs in 48 broiler rabbits fed diets containing 0, 5, 10 and 20% level of neem seed kernal cake (NSKC) for 6 weeks and subsequently sacrificed. The NSKC incorporation in the diet did not exert any adverse effect on caecal fermentation although the weight of caecum and its contents was significantly (p<0.01; p<0.05) lower in rabbits fed the diet containing 20% NSKC. Except for blood glucose concentration, none of the blood biochemical constituents (serum urea nitrogen, creatinine, total protein, haemoglobin) and the activities of different enzymes (alanine aminotransferase, asparatate aminotransferase, alkaline phosphatase) varied significantly due to NSKC feeding. A histopathological examination of the vital organs (liver, kidney, heart, spleen, lungs, intestine and stomach) revealed a variable degree of villus atrophy in the intestine and degenerative changes in the liver and tubular epithelium of kidney in some rabbits when NSKC was fed at levels above 10%.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.609-613
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• 1997

The reproductive performance of forty two male broilers divided into three similar groups and fed on isocaloric and isonitrogneous diets containing 0, 10 or 20% water washed neem seed kernel meal (WWNSKM) was investigated from 20 to 32-wks of age. Results on semen characteristics revealed that feeding of WWNSKM led to significant (p < 0.05) reduction in semen volume, sperm concentration associated with increased incidences of morphological abnormalities in the spermatozoa when compared to that of the control birds. A drastic reduction in the fertilizing ability of spermatozoa was observed, the adverse effects being more at higher inclusion level of the cake. Hatchability of eggs also declined in the WWNSKM fed group. Histological examination of testes revealed a higher number of degenerating cells and poor spermatogenesis along with multinucleated giant cells in the seminiferous tubules of the testes of birds receiving the high dose of WWNSKM in diet. It may be concluded that the feeding of WWNSKM by incorporating in isocaloric and isonitrogneous diets to cockerels is associated with adverse effect on their fertility.