• Journal of Yeungnam Medical Science
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• 제13권2호
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• pp.243-250
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• 1996

실험용 쥐를 이용하여 항암 약제인 mitomycin-c를 피하 주사하여 인위적으로 혈관외 유출을 시켜 조직 괴사를 유발하였다. 조직 괴사를 예방하기 위하여 치료 시작시간에 따른 dimethyl sulfoxide의 국소 도포와 sodium thiosulfate의 진피내 주사의 치료 효과를 비교하고 조직학적 검사를 실시하여 다음과 같은 결과를 얻었다. 1. Mitomycin-C가 혈관외로 유출될 경우 dimethyl sulfoxide를 6시간 이내에 국소 도포하면 괴사를 예방할 수 있었고, 24시간 이내에 치료를 시작해도 괴사 면적에 유의한 감소를 가져올 수 있었다(p<0.01). 2. Sodium thiosulfate 진피내 주사를 할 경우에는 12시간 이내에는 괴사를 예방할 수 있었으나 24시간이 경과한 후에는 치료 효과가 없었다(p<0.01). 3. MMC에 의한 괴사는 비균성 응고성 괴사였으며, 염증 반응이나 육아 조직은 나타나지 않았으며, 국소 도포와 진피내 주사 방법에 따른 조직학적 변화를 제외한 차이는 없었다. 4. MMC의 혈관외 유출로 피부병변을 최소화 시킬 수 있는 방법은 DMSO와 STS의 즉시 병합치료가 좋은 효과를 얻을 수 있을 것으로 추측된다.

• 대한치과의사협회지
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• 제15권12호
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• pp.957-962
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• 1977

The author has observed the tissue reaction of the absolute alcohol infection of rat oral mucosa. 0.5ml absolute alcohol was injected subcutaneously on the mucobuccal fold of rat. And the rats were sacrifieced at intervals of one day, 3rd, 1 week, 2 week and 4 week after alcohol injection. The microscopic tissue sections were made and stained with hematoxylin and eosin. The results were are as follows; 1. Degeneration and shrinkage of fibroblasts and coagulative necrosis were observed one day to and three day after alcohol injection. 2. Although coagulative necrosis and tissue degeneration occurred, the inflammatory infiltration was not prominent especially there were scarcely any polymorphonuclear leukocytes in that field. 3. Granulation tissue with moderate small round cell infiltration were replaced the necrotic area at one week after injection and the fibroblast proliferate into the granulation tissue at two week group. 4. At four week after injection, the damaged area recovered by fibroblastic proliferation and collage formation, but there were

• 대한수의학회지
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• 제56권1호
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• pp.41-43
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• 2016

A dead dove was found on the road and submitted for diagnosis. The bird was severely emaciated, with deformation in its facial area. Grossly, white coalescing nodules were seen on the cut surface of the nasal cavity. Histopathologically, epithelial cells of the upper respiratory tract were markedly proliferated, with ballooning degeneration, down growth of the rete ridge, and large eosinophilic intracytoplasmic inclusion bodies. Parakeratotic hyperkeratosis and focal necrotic focus was present in the proliferative area. The facial bones showed partial bone resorption. Transmission electron microscopy revealed numerous viral particles in epithelial cells with dumbbell-shaped bodies, consistent with poxvirus.

• 한국식물병리학회지
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• 제14권5호
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• pp.433-439
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• 1998

The studies on the ecology of virus disease on Chinese cabbage (Brassica campestris subsp. pekinensis) cultivated in alpine area of Kangwon province during summer season to analyse its influence on damage and develope a prediction model were performed from 1993 to 1997. Virus disease on Chinese cabbage occurring in the alpine area showed various symptom types and among there, necrotic spots and dwarf were mainly detected. The disease was increased from early August and continued mid September in every year. The occurrence of virus disease was the highest in 1994 with 20.5%, and the number of aphid vectors were also the highest during the same period. The number of aphids in the alpine areas showed twice peaks every year. For the analysis of damage by virus infection, the infection and injured ratio of all treatments were more than 90% and 80%, respectively. The most important factor for the occurrence of virus disease on Chinese cabbage was temperature. Factors influencing the development of the viral disease in the alpine area were maximum temperature and number of aphid vectors.

• Toxicological Research
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• 제21권2호
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• pp.141-150
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• 2005

1,3-Dichloro-2-propanol (1,3-DCP) is known as chloride chemicals and causes severe hepatotoxic agent. The Ito cells and Kupffer's cells of the liver in the 5 old F344 Rats were exposed to 1,3-DCP gas chamber for 6 hours/ a day, 5 days/ a week, and 13 weeks, in the 0, 5, 20, 80 ppm, respectively. After then the body weights, liver weights, and relative liver weight to body weight were measured, and the hepatic tissues were prepared by the routine and Immunostain method, and observed by the LM, and EM. In the results, there were severe body weight decrease (p<0.05) in the 80 ppm of the male and female rats. The relative liver weights to the body weight were increased relate with exposed 1,3-DCP concentration (P<0.001). Inflammatory cells, infiltration was observed at the perivascular area in the 20 ppm exposed group, and bilirubin pigment infiltration, bile duct hyperplasia, inflammation hepatocytic necrosis, fibrosis were observed in the 80 ppm exposure group. In the 80 ppm exposure group, disarrangement of the endothelial cells, erythrocytes and hepatic cell fragment in the Disse space and numerous migration macrophages were observed in the necrotic area by EM observation. In the immunostained hepatic tissues positive stained ED1 cells were extremely increased (P<0.05) in central vein area, but ED2 was weakly positive immunostained in the 80 ppm exposed group. Immunostained desmin was observed in the Ito cell. It was no difference in the low and medium exposed group but it was typical increase in the necrotic area. In conclusion, These results suggest that NOAEL of 1,3-DCP may be 5 ppm in rats and the Immunostained of desmin, ED1 and ED2 positive cells activated in the inflammatory liver were related to the exposure volume and density. The increase of the Ito cells were related to the severe phagocytosis of the Kupffer's cells.

• 대한두개안면성형외과학회지
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• 제19권1호
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• pp.64-67
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• 2018

Varying degrees of complications can occur after hyaluronic acid filler injections. Tissue necrosis due to interruption of the vascular supply is an early complication that can be severe. If the site of tissue necrosis due to the filler injection is the forehead, successfully reconstructing the region without distorting the key landmarks is challenging. We describe the case of a 50-year-old man who experienced widespread forehead skin necrosis after hyaluronic acid filler injection in the glabellar area. We successfully covered the forehead area with a $3{\times}4-cm^2$ midline necrotic tissue using the modified double-opposing rotation-advancement flap method. Although modified double-opposing rotation-advancement flap closure has the disadvantage of leaving a longer scar compared to conventional double-opposing rotation-advancement flap closure, the additional incision line made along the superior border of the eyebrow aids in camouflaging the scar and decreases eyebrow distortion. Therefore, it is believed that the modified double-opposing rotation-advancement flap technique is an excellent tool for providing adequate soft tissue coverage and minimal free margin distortion when reconstructing widespread skin necrosis in the central mid-lower forehead that can occur after filler injection in the glabellar area.

• 식물병연구
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• 제16권3호
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• pp.232-237
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• 2010

Tomato spotted wilt virus(TSWV)의 상습 발생지인 안양지역에서 가지, 알타리무, 슈가로프에서 TSWV가 발생하였다. 알타리무에서는 잎에 괴저반점과 뿌리에 괴저 병징이 나타났다. 가지에서는 잎에 전형적인 다중 원형반점을 나타냈으며 열매에 심한 괴저를 나타냈다. 슈가로프에서는 잎에 전형적인 원형반점과 심한 위축 병징이 나타났다. 가지, 알타리 무, 슈가로프에서 분리한 TSWV의 생물적 특성은 흰 명아주, 붉은 명아주, 담배(N. devney)에서는 국부 감염이었으며, 담배(N. glutinosa, N. benthamiana)와 독말풀(D. stramonium)에서는 전심감염이었다. 가지와 슈가로프에서 분리한 TSWV는 병원성이 유사하였으나, 알타리 무에서 분리한 TSWV는 N. tabacum 'Xanthi NC' 등 5종의 담배에서 국부 감염되어 병원성이 매우 상이하였다.

• 식물병연구
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• 제14권2호
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• pp.134-137
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• 2008

경북 청도지역의 단 고추 과일에 나타난 큰 괴저 반점 및 과일 괴저와 잎에 엽맥 녹대 및 기형 병징을 일으키는 바이러스는 오이모자이크바이러스의 새로운 계통인 CMV-NP로 분류동정되었다. CMV-NP는 직경이 26 nm의 구형이었으며, VC/RT-PCR 유전자 진단결과 CMV로 확인되었다. CMV-NP는 오이(Cucumis sativus) 등 9개 지표식물에 전신감염을 일으켰으며, 명아주(Chenopodim amaranticolor; C. quinoa)에 국부감염을 일으켰다. CMV-NP는 담배(N. rustica)와 번행초(Tetragonia expansa)의 접종잎과 상엽에 특이한 괴저 원형 반점을 일으켰다. 특히 오이에서는 상엽에 큰 퇴록 원형반점과 엽맥퇴록 병징을 일으켰으며 독말풀(Datura stramonium)에서는 병원성이 없었다.

• The Plant Pathology Journal
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• 제26권4호
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• pp.328-339
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• 2010

A tobamovirus, Ribgrass mosaic virus (RMV), was identified newly from chinese cabbage (Brassica campestris L. pekinensis) in Korea. Virus disease incidence of RMV on chinese cabbage was 37.9% in alpine area on August in 1993. RMV induced the symptoms of necrotic ring spots, necrotic streak on midrib and malformation. RMV, Ca1 and Ca3 isolate, could infect 35 species out of 45 plants including Chenopodium amaranticolor. Physical properties of RMV Ca1 isolate were very stable as 10.8 over for dilution end point, $95^{\circ}C$ for temperature inactivation point and 18 weeks for longevity in vitro. RMV had the soil transmission rate of 75.0% for the chinese cabbages, 'Chunhawang' and 'Seoul' cultivars. The purified virions of RMV had the typical ultraviolet absorption spectrum of maximum at 260 nm and minimum at 247 nm. RMV of Ca1 isolate was related serologically with antisera of Tobacco mosaic virus (TMV)-Cym, TMV-O and Pepper mottle virus, but not related with antiserum of Odontoglossum ring spot virus. coat protein gene of RMV-Ca1, sized 473 nucleotides, encoded 158 amino acid residues. Nucleotide identity of RMV-Ca1 CP gene was 96.4% with RMV-Shanghai (GenBank accession No. of AF185272) from China and 96.0% with RMV-Impatiens (GenBank accession No. of AM040974) from Germany. Identity of amino acids between RMV-Ca1 and the two RMV isolates was 96.8%. Specific three primers were selected for rapid and easy genetic detection of RMV using Virion Captured (VC)/RT-PCR method.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제47권2호
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• pp.99-111
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• 2021

Objectives: It is unclear whether the extent of intraoral mucosa defects in patients with medication-related osteonecrosis of the jaw indicates disease severity. Therefore, this study investigated whether mucosal lesions correlate with the true extent of osseous defects in stage I patients. Materials and Methods: Retrospectively, all patients with stage I medication-related osteonecrosis of the jaw who underwent surgical treatment between April 2018 and April 2019 were enrolled. Preoperatively, the extent of their mucosal lesions was measured in clinical evaluations, and patients were assigned to either the visible or the probeable bone group. Intraoperatively, the extent of necrosis was measured manually and with fluorescence. Results: Fifty-five patients (36 female, 19 male) with 86 lesions (46 visible bone, 40 probeable bone) were enrolled. Intraoperatively, the necrotic lesions were significantly larger (P<0.001) than the preoperative mucosal lesions in both groups. A significant (P<0.05) but very weak (R2<0.2) relationship was noted between the extent of the mucosal lesions and the necrotic bone area. Conclusion: Preoperative mucosal defects (visible or probeable) in patients with medication-related osteonecrosis of the jaw do not indicate the extent of bone necrosis or disease severity.