• Title/Summary/Keyword: Near work

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The effect of Swd2's binding to Set1 on the dual functions of Swd2 in Saccharomyces cerevisiae (Saccharomyces cerevisiae의 Swd2와 Set1의 결합이 Swd2의 이중적인 기능에 미치는 영향)

  • Park, Shinae;Lee, Jung-Shin
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.286-291
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    • 2017
  • In eukaryotic cells, histone modification is an important mechanism to regulate the chromatin structure. The methylation of the fourth lysine on histone H3 (H3K4) by Set1 complex is one of the various well-known histone modifications. Set1 complex has seven subunits including Swd2, which is known to be important for H2B ubiquitination dependent on H3K4 methylation. Swd2 was reported to regulate Set1's methyltransferase activity by binding to near RNA recognition motif (RRM) domain of Set1 and to act as a component of CPF (Cleavage and Polyadenylation Factors) complex involved in RNA 3' end processing. According to the recent reports, two functions of Swd2 work independently of each other and the lethality of Swd2 knockout strain was known to be caused by its function as a component of CPF complex. In this study, we found that Swd2 could influence the Set1's stability as well as histone methyltransferase activity through the association with RRM domain of Set1. Also, we found that ${\Delta}swd2$ mutant bearing truncated-Set1, which cannot interact with Swd2, lost its lethality and grew normally. These results suggest that the dual functions of Swd2 in H3K4 methylation and RNA 3' end processing are not independent in Saccharomyces cerevisiae.

Studies on the tolerance of Halophyte Arabis stelleri under heavy metals and Salt stress condition (염생식물 섬갯장대(Arabis stelleri var. japonica)의 중금속 및 고염 농도 스트레스 상태에서 내성 연구)

  • Kim, Donggiun
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.4
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    • pp.373-378
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    • 2019
  • In the marine area, the salt concentration in the soil increases, and the inland heavy metal pollution increases the damage of plants. In the inland industrial development area, researches on the genetic resources of plants together with the heavy metal accumulation of Co, Ni, Zn, and so on are required. Both of these problems have caused scientists to work hard to find plants that are likely to cause stress in plant roots. In this study, seeds of Arabis stelleri var. japonica collected near the shore were used for germination. The growth and development and tolerance of both Arabis and Arabidopsis seeds were investigated under laboratory culture conditions. As a result, Arabis showed resistance about 3 times in 250 mM nickle and cobalt, and more than 4 times in 1 mM zinc when compared to Arabidopsis. The tolerance of Arabis to Na salts increased by 20% or more at 50 mM concentration and Arabis was resistant to heavy metals and salt concentration. The accumulation of Na ions in the body was measured as a preparation for studying the intracellular mechanism. As a result, it showed a further decrease in resistance to ground water roots. It is considered that the activity of the exporting gene is important rather than the mechanism of accumulation.

Comparative Analysis of Rice Lodging Area Using a UAV-based Multispectral Imagery (무인기 기반 다중분광 영상을 이용한 벼 쓰러짐 영역의 특성 분석)

  • Moon, Hyun-Dong;Ryu, Jae-Hyun;Na, Sang-il;Jang, Seon Woong;Sin, Seo-ho;Cho, Jaeil
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.917-926
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    • 2021
  • Lodging rice is one of critical agro-meteorological disasters. In this study, the UAV-based multispectral imageries before and after rice lodging in rice paddy field of Jeollanamdo agricultural research and extension servicesin 2020 was analyzed. The UAV imagery on 14th Aug. includesthe paddy rice without any damage. However, 4th and 19th Sep. showed the area of rice lodging. Multispectral camera of 10 bands from 444 nm to 842 nm was used. At the area of restoration work against lodging rice, the reflectance from 531 nm to 842 nm were decreased in comparison to un-lodging rice. At the area of lodging rice, the reflectance of around 668 nm had small increases. Further, the blue and NIR (Near-Infrared) wavelength had larger. However, according to the types of lodging, the change of reflectance was different. The NDVI (Normalized Difference Vegetation Index) and NDRE (Normalized Difference Red Edge) shows dome sensitivities to lodging rice, but they were different to types of lodging. These results will be useful to make algorithm to detect the area of lodging rice using a UAV.

Development of Prediction Model for Sugar Content of Strawberry Using NIR Spectroscopy (근적외선 분광을 이용한 딸기의 당도예측모델 개발)

  • Son, Jaeryong;Lee, Kangjin;Kang, Sukwon;Yang, Gilmo;Seo, Youngwook
    • Food Engineering Progress
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    • v.13 no.4
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    • pp.297-301
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    • 2009
  • This study was performed to develop a prediction model of sugar content for strawberry. Near-infrared (NIR) spectroscopy has been prevailed for on-line and portable applications for non-invasive quality assessment of intact fruit. This work presents effects of illumination method and coating of reflection surface of light source on prediction result of sugar content. Effect of preprocessing methods was also examined. A low-cost commercially available VIS/NIR spectrometer was used for estimation of total soluble solids content (Brix). To predict sugar contents of strawberry, the best results were obtained with the spectrum data measured under intensive illuminations at three locations induced from the light source with fiber optic bundles. Gold coating of reflection surface of light source lamp gave favorable effect to prediction result. The best results in validation of PLSR model were $r_{SEP}$ = 0.891 and SEP = 0.443 Brix under OSC preprocessing and those of PCR were $r_{SEP}$ = 0.845, SEP $r_{SEP}$= 0.520 Brix, under no preprocessing.

A Case Study on the Construction at Near Verge Section of Secure Objects Using Electronic Detonators (전자뇌관을 이용한 보안물건 초근접구간 시공 사례)

  • Hwang, Nam-Sun;Lee, Dong-Hee;Lim, Il-soo;Kim, Jin-soo
    • Explosives and Blasting
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    • v.37 no.2
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    • pp.22-30
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    • 2019
  • On sites where explosives are used, the effects of noise and vibration produced by the blast wave are subject to a number of operational restrictions. Recently, the number of civil complaints has increased and the standard of environmental regulations on secure goods has been greatly tighten. Therefore, work is generally carried out by machine excavation in case of close proximity of safety thing. Machine excavation methods have the advantage as reducing noise and vibration compared to blasting methods, but depending on the conditions of rock intended to be excavated, they are sometimes less constructive than planned. In general, the closer a rock type is to hard rock, the less constructible it becomes. In this paper, we are going to explain the construction of a construction section with a close proximity to a safety thing using electronic detonators. While the project site was designed with a machine excavation methods due to the close(9.9m) proximity of safety thing(the railroad), construction using electronic detonators was reviewed as an alternative method for improving rate of advance time and construction efficiency when expose to hard rock. Through blasting using electronic detonators, construction and economic efficiency were maximized while minimizing impact on surrounding safety things. Because $HiTRONIC^{TM}$, which is produced by Hanwha, has innovative stability and high explosion reliability, it is able to explode with high-precision accuracy. Electronic detonators are widely used in construction sites of railway or highway, other urban burrowing areas and large limestone mines.

Exposure to Atmospheric Particulates and Associated Respirable Deposition Dose to Street Vendors at the Residential and Commercial Sites in Dehradun City

  • Prabhu, Vignesh;Gupta, Sunil K.;Madhwal, Sandeep;Shridhar, Vijay
    • Safety and Health at Work
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    • v.10 no.2
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    • pp.237-244
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    • 2019
  • Background: Street vendors spend relatively more time near roadways and are vulnerable to air pollution related health disorders. However, there is limited information on the quality of the air they breathe. The objectives of this present study were to calculate the mass concentration of atmospheric particulate matter (PM) in eight size fractions ($PM_{0.4-0.7}$, $PM_{0.7-1.1}$, $PM_{1.1-2.1}$, $PM_{2.1-3.3}$, $PM_{3.3-4.7}$, $PM_{4.7-5.8}$, $PM_{5.8-9.0}$, and $PM_{9.0--0{\mu}m}$) at commercial (CML) and residential site (RSL) in Dehradun city from November 2015 to May 2016. To estimate the corresponding respiratory deposition dose (RDDs) in alveolar (AL), tracheobronchial (TB), and head airway (HD) region on street vendors working at CML and RSL. To find the association of atmospheric PM with RDDs and the incidence of respiratory related disorders among street vendors. Methods: Andersen cascade impactor was employed for calculating the PM mass concentration. Questionnaire based health survey among street vendors were carried out through personal interview. Results: A significant difference (p < 0.05; t-test) between the mean $PM_{0.4-10{\mu}m}$ mass concentration at CML and RSL was observed with ($mean{\pm}SD$) $84.05{\pm}14.5$ and $77.23{\pm}11.7{\mu}g\;m^{-3}$, respectively. RDDs in AL, TB and HD region at CML was observed to be 9.9, 7.8, and 7.3% higher than at RSL, respectively. Health survey revealed 1.62, 0.96, 0.04, and 0.57 times higher incidence of cold, cough, breathlessness, and chest pain, respectively with street vendors at CML compared to RSL. Conclusion: The site characteristics plays a major role in the respiratory health status of street vendors at Dehradun.

Preliminary Research of the Sedimentary Environment in Bupyeng Reservoir Region, Soyang Lake in Chuncheon - Focus on Sentinel-2 Satellite Images and in-situ data - (춘천시 소양호 상류 부평지구의 퇴적환경에 대한 선행연구 - 현장조사와 위성영상자료를 중심으로 -)

  • Kim, GeonYoung;Kim, Dain;Kim, TaeHun;Lee, JinHo;Jang, YoSep;Choi, HyunJin;Shim, WonJae;Park, SungJae;Lee, Chang-Wook
    • Korean Journal of Remote Sensing
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    • v.34 no.6_1
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    • pp.1119-1130
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    • 2018
  • Soyang Lake has been contributing to economic growth by preventing flood damage in the metropolitan area, the water level in the middle to upper flow of lake has been greatly decreased due to the drought in 2015. In order to restore the existing flow rate, Bupyungbo has been built in Bupyeong-ri, Shin Nam-myeon, Inje-gun to cause artificial changes on the sedimentary environment of Bupyeong freshwater region. Therefore, this study intends to confirm the changes of sedimentary environment since Bupyeongbo has been utilized. For this study, we used the Sentinel-2 satellite image data periodically to measure the dimension of water according to the volume of water kept near Bupyung district and analyzed the particle size and the percentage of water content of the sediments through field study. The Sentnel-2 satellite images showed us how the water surface has been changed and that during the period from September 2017 to October 2018, the minimum and maximum area of water surface was observed in June 2018 and in January 2018, respectively. In addition, we find that the smaller being the particle size, the higher having the water content and that there is higher the correlation between the water content and the grain size of the sediment layer. Hereafter, if we will acquire the drone images at Bupyung district, we expect that we will be able to measure the distribution of sediments in the same area according to different time periods and observe various kinds of sediment through field work.

Heat Transfer Modeling by the Contact Condition and the Hole Distance for A-KRS Vertical Disposal (A-KRS 수직 처분공 접촉 조건 및 처분공 간의 거리에 따른 열전달 해석)

  • Kim, Dae-Young;Kim, Seung-Hyun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.17 no.3
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    • pp.313-319
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    • 2019
  • The A-KRS (Advanced Korean Reference Disposal System) is the disposal concept for pyroprocessed waste, which has been developed by the Korea Atomic Energy Research Institute. In this disposal concept, the amount of high-level radioactive waste is minimized using pyrochemical process, called pyroprocessing. The produced pyroprocessed waste is then solidified in the form of monazite ceramic. The final product of ceramic wastes will be disposed of in a deep geological repository. By the way, the decay heat is generated due to the radioactive decay of fission products and raises the temperature of buffer materials in the near field of radioactive waste repository. However, the buffer temperature must be kept below $100^{\circ}C$ according to the safety regulation. Usually, the temperature can be controlled by variation of the canister interdistance. However, KAERI has modelled thermal analysis under the boundary condition, where the waste canisters are in direct contact with each other. Therefore, a reliable temperature analysis in the disposal system may fail because of unknown thermal resistence values caused by the spatial gap between waste canisters. In the present work, we have performed thermal analyses considering the gap between heating elements and canisters at the beginning of canister loading into the radioactive waste repository. All thermal analyses were performed using the COMSOL software package.

Distribution Aspects and Extinction Threat Evaluation of the Korean Endemic Species, Iksookimia pacifica (Pisces: Cobitidae) in Korea (한국고유종 북방종개 Iksookimia pacifica (Pisces: Cobitidae)의 분포양상과 멸종위협 평가)

  • Ko, Myeong-Hun;Han, Mee-Sook;Kwan, Sun-Man
    • Korean Journal of Ichthyology
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    • v.31 no.1
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    • pp.16-22
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    • 2019
  • Investigations on the study of the distribution aspects and extinction threat evaluation of the Korean endemic species, Iksookimia pacifica were done from 2017 to 2018 in Korea. During the study period, the samples of I. pacifica were collected in 17 streams, 46 sites (from Baebongcheon Stream of Goseong-gun to Gunsuncheon Stream of Gangneung-si) among the noted 33 streams and 104 sampling sites investigated. The population size of I. pacifica was relatively large in streams such as Bukcheon, Baebongcheon, Hwasangcheon, Cheonjincheon, Ohhocheon Stream etc., but the population size was small in streams such as Sacheoncheon, Namcheon, Gangneung Namdaecheon Stream etc. The main habitat of I. pacifica was the downstream pool of clean water with slow velocity and sand bottoms, and their sensibility was estimated to be due to river work and water pollution. Comparing the previous records of the appearance of I. pacifica, they were first seen in Sampocheon Stream, but they did not appear in Jusucheon, Jeoncheon, Samcheok Osipcheon Stream. Given this evidence as noted for the 19.5% reduction in occupancy within 3 generations, in small appearance range ($1,343km^2$) and small occupancy area ($184km^2$), the number of locations were many (18) and the population was relatively large within the range of habitat. Therefore, I. pacifica is now considered a Near Threatened (NT) based on the IUCN Red List categories and criteria.

A Study on the Practical Methodology of Engineering Education through Multi-account Service Composition with Enhanced User Security (사용자 보안을 강화한 다중 계정 서비스 구성을 통해 이루어진 공학 교육 실천 방법론에 관한 연구)

  • Kang, Kyoung-Bo;Park, Jea-Soon;Chang, Eun-Young
    • Journal of Practical Engineering Education
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    • v.10 no.1
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    • pp.1-7
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    • 2018
  • The dual security system that manages user's account information and uses NFC and OTP has been proposed. It also enhances the security of the user authentication phase by using the features that recognize within the range of less than 1m of NFC and the high security of NFC itself. During the graduation work design process which was carried out for one year and six months in a group of two in the electronics engineering major of the four-year undergraduate system was conducted. In the interim results, we participated in the conferences and thesis contest at the Institute of related to major for three times. Finally passed the examination process for three times judged by 6 professors in the major. The results were published in a graduation thesis together with other people who passed and composed in thesis form. Through the experience gained through these stages of design and production, it has gained the confidence to gain employment opportunities and actively pursue the growth stage, and presented a methodology to practice engineering education through employment.