• Title/Summary/Keyword: Natural source

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Biodegradation of Phenol by a Trichloroethylene-cometabolizing Bacterium

  • Park, Geun-Tae;Son, Hong-Joo;Kim, Jong-Goo;Lee, Sang-Joon
    • Journal of Microbiology and Biotechnology
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    • v.8 no.1
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    • pp.61-66
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    • 1998
  • A microorganism which degrades phenol and co-metabolizes trichloroethylene (TCE) was isolated from Yangsan stream after enrichment in a medium containing phenol as the sole carbon source. The isolate EL-43P was identified as the genus Rhodococcus by its morphological, cultural and physiological characteristics. Phenol-induced cells of Rhodococcus sp. EL-43P degraded TCE. Toluene and nutrient broth could not replace the phenol requirement. The optimal conditions of initial pH and temperature of media for growth were 7.0~9.0 and $30~50^{\circ}C$, respectively. Rhodococcus sp. EL-43P could grow with phenol up to 1,000 ppm. Growth was inhibited by phenol at a concentration above 1,500 ppm. It was observed that Rhodococcus sp. EL-43P was able to degrade 90% of phenol (1,000 ppm) after 40 h in a culture. Phenol-induced cells of Rhodococcus sp. EL-43P degraded 95% of $5{\mu}M$ TCE in 6 h. Rhodococcus sp. EL-43P hardly degraded TCE above $100{\mu}M$.

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A Prototype Virtual Network Embedding System using OpenStack

  • Fukushima, Yukinobu;Sato, Kohei;Goda, Itsuho;Ryu, Heung-Gyoon;Yokohira, Tokumi
    • IEIE Transactions on Smart Processing and Computing
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    • v.6 no.1
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    • pp.60-65
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    • 2017
  • Network virtualization enables us to make efficient use of resources in a physical network by embedding multiple virtual networks in the physical network. In this paper, we develop a prototype of a virtual network embedding system. Our system consists of OpenStack, which is an open source cloud service platform, and shell scripts. Because OpenStack does not provide a quality of service control function, we realize bandwidth reservation for virtual links by making use of the ingress policing function of Open vSwitch, which is a virtual switch used in OpenStack. The shell scripts in our system automatically construct the required virtual network on the physical network using the OpenStack command-line interface, and they reserve bandwidth for virtual links using the Open vSwitch command. Experimental evaluation confirms that our system constructs the requested virtual network and appropriately allocates node and link resources to it.

A legal regime to govern the exploitation of the natural resources of the Moon and other celestial bodies

  • Tronchetti, Fabio
    • 한국항공우주법학회:학술대회논문집
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    • 2008.05a
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    • pp.185-215
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    • 2008
  • The exploitation of the natural resources of the Moon and other celestial bodies represents one of the most exiting future developments in the field of space law as well as a unique occasion for the economic and social growth of mankind as a whole. The large number of benefits that are expected to be generated from the exploitation of these resources, indeed, not only will contribute to the betterment of conditions of people on Earth but also will allow mankind to face and likely solve one of the biggest problems currently affecting our planet, namely the exhaustion of the stocks of raw materials and other source of energy, such as fossil fuels. The exploitation of the natural resources of the Moon and other celestial bodies, however, has been prevented so far by the absence of dedicated space law rules allowing its orderly and peaceful development and clarifying the rights and duties of the parties involved in it. Due to the uncertainty generated by the absence of these rules, indeed, States as well as private operators have refrained from investing in the exploitation of space resources so far. The time to change this situation and to allow the exploitation of extraterrestrial resources to begin has finally come. This paper aims at fulfilling this purpose by proposing a legal regime containing specific and detailed rules to regulate the exploitation of the natural resources of the Moon and other celestial bodies.

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Nutritional Value of a Heterotrichous Ciliate, Fabrea salina with Emphasis on Its Fatty Acid Profile

  • Pandey, B.D.;Yeragi, S.G.;Pal, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.995-999
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    • 2004
  • Fabrea salina is a hypersaline ciliate having importance as a live food source for juvenile stages of aquatic animals including smaller invertebrates. The analysis of this ciliate for proximate and biochemical composition was carried out. The moisture, protein, fat, carbohydrate and ash content of F. salina from natural sources were 86.66$\pm$0.380, 56.66$\pm$0.494%, 36.66$\pm$0.614%, 1$\pm$0.073% and 4$\pm$0.182%, respectively. Gas chromatographic analysis (percent area below the curve) revealed that the presence of oleic acid was higher over other fatty acids in both natural and cultured F. salina. The absolute content of oleic acid was higher in natural (18.91% area) than in the cultured (10.74% area) F. salina. Linoleic and linolenic acids were also among major fatty acids with the percentage area of 16.29 and 14.58, respectively. The number of fatty acids in cultured Fabrea was less as compared to the natural ones and the oleic acid was followed by palmitic acid, palmitoleic acid, linoleic acid and stearic acid.

Emulsification of Crude Oil by Acinetobacter sp. SH-14

  • Son, Hong-Joo;Go, Sun-Hee;Lee, Geon;Lee, Sang-Joon
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.363-369
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    • 1996
  • As basic study to evaluate the treatability of oil-contaminated environment with bacteria, isolation and characterization of crude oil-degrading bacterium were carried out. A bacterial strain SH-14 capable of degrading crude oil was isolated from contaminated soils by enrichment culture technique and identified as Acinetobacter sp. by morphological, cultural and biochemical characteristics, and so named Acinetobacter sp. SH-14. The optimal medium composition and cultural conditions for the growth and emulsification of crude oil by Acinetobacter sp. SH-14 used were crude oil of 2.0%, $KNO_3$ of 0.2%, $K_2HPO_4$ of 0.05%, and $MgSO_4\;{\cdot}\;7H_2O$ of 1.0%, along with initial pH 7.0 at $30^{\circ}C$. Acinetobacter sp. SH-14 showed to be resistant to chloramphenicol and utilized various hydrocarbons such as dodecane, hexadecane, isooctane, cyclo-hexane etc., as a sole carbon source. Acinetobacter sp. SH-14 harbored a single plasmid. By agarose gel electrophoresis and curing experiment it was found that the genes for crude oil components degradation were encoded on the plasmid.

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Purification and Characterization of an Indican-hydrolyzing β-glucosidase from Agrobacterium tumefaciens (Agrobacterium tumefaciens 유래 인디칸 분해활성을 갖는 β-glucosidase의 분리와 특성분석)

  • Hwang, Chang-Sun;Lee, Jin-Young;Kim, Geun-Joong
    • KSBB Journal
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    • v.27 no.6
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    • pp.341-346
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    • 2012
  • Indican (indoxyl-${\beta}$-D-glucoside) is a colorless natural compound and can be used as a precursor for the production of indigo. This production step only require an enzyme, ${\beta}$-glucosidase, that readily screened from microbial resource by using selective media supplemented with indican as a sole carbon source. Agrobacterium tumefaciens was well grown in this media and thus presumed to produce a related enzyme. The corresponding gene, encoding a protein with a calculated molecular mass of 51 kDa, was cloned and overexpressed as MBP fusion proteins. The purified enzyme was determined to be a dimer and showed the maximum activity for indican at pH 7.0 and $40^{\circ}C$. The kinetic parameters for indican, Km and Vmax, were determined to be 1.4 mM and 373.8 ${\mu}M/min/mg$, respectively. The conversion yield of indican into indigo using this enzyme was about 1.7-1.8 folds higher than that of previously isolated enzyme from Sinorhizobium meliloti. Additionally, this enzyme was able to hydrolyze various ${\beta}$-1,4 glycoside substrates.

Study of Antioxidative Substances from Some Indonesian Plants

  • Cahyana, Herry
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1998.11a
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    • pp.162-162
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    • 1998
  • Various natural compounds act as antioxidants in protection against lipid peroxidation. Lipid peroxidation yields a variety of decomposition products which have been implicated in not only decreasing the nutritional value of food, but also in developing an off-flavor and toxic substances. As a source of safer and more effective natural antioxidants of natural origin have been widely investigated. Some Indonesian plants have evaluated for their antioxidative activity, and suggested the possible existence of various antioxygenic compounds in them. We attempted to study such antioxygenic compounds with simple method evaluation. As we are interested in the natural product compounds, we examined of several sample such as edible sea-weeds, and some edible fruits. Sea-weed, Eisenia bicyclis, one of the edible brown algae, exhibited the activity. As a traditional food additive consumed by Indonesian, Garcinia parvifolia is used as taste supplement in region West Sumatra, have been studied. Our current studies on the semi-polar fractions shows the activity by the thiocyanate method test. Another sample, Garcinia mangostana, a famous fruit with sweet taste, the part kernel have also evaluated. The acidic fraction of the extract showed antioxidative activity. Some other active components were found in the neutral and BuOH fractions.

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The Exchange of Reduced Sulfur Gases Across the Atmosphere-Teerrestrial Biosphere Interface

  • Kim, Ki-Hyun;Zhen Yand;Shiming Wang
    • Journal of Korean Society for Atmospheric Environment
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    • v.12 no.E
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    • pp.1-18
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    • 1996
  • In this review, the significance of terrestrial ecosystems in the global sulfur budget has been reviewed based on the currently available databases covering the topic. In the section 1, we describe our current understanding of natural sulfur cycle in relation to most well-known natural reservoir, oceanic environment. The sections 2 and 3 provide the fundamental pictures of the rerrestrial sulfur cycle with respect to the relative importance of its individe the fundamental pictures of the terrestrial sulfur cycle with respect to the section 3, previously reported flux values for several major sulfur gases are presented for each reservoir and are intercompared to derive representative fluxes for the respective environment. In the section 4, source mechanisms for volatile sulfur species are dealt for both microscale and macroscale processes leading to their productions. In the section 5, environmental factors controlling the exchange of biogenic sulfur gases across the air-surface have been discussed. In the section 6, environmental fate of sulfur gases released into the atmosphere has been described. Finally in tie section 7, as concluding remarksm we discuss directions and suggestions to overcome various limitations encountered from previous measurement investigations of natural sulfur cycle in diverse natural ecological systems.

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Corporation's Adaptation to Climate Change Related Natural Disasters : Embedding Resiliency in Supply Chain - A Study on Climate Change Related Natural Disaster Adaptation for Corporations -

  • Pak, Myong Sop;Kim, In Sun
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.64
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    • pp.239-264
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    • 2014
  • Two types of responses to climate change exist. First is climate mitigation which includes efforts of reducing CO2 and GHG emissions. Second response is climate adaptation process which is establishing climate resilience in the supply chain. The two are inherently different since mitigation strategy focus on eliminating the source of climate change and is long term in nature but adaptation strategy is moderating the impact of potential or current climate change. In order to embed climate resilience in the supply chain, mitigation strategies and adaption strategies must be implemented simultaneously. Corporation's adaptation to climate change related natural disaster can be seen as a response that includes mitigation and adaptation strategies simultaneously. A comprehensive climate change resilience supply chain approach has to be developed. This paper illustrated guidelines and adaptation process framework businesses can utilize in order to build climate resilience. Screening process before the actual assessment of risk was introduced as well as the whole adaptation process of establishing information system and strengthening climate-related operational flexibility.

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A legal regime to govern the exploitation of the natural resources of the Moon and other celestial bodies

  • Tronchetti, Fabio
    • The Korean Journal of Air & Space Law and Policy
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    • v.23 no.1
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    • pp.131-168
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    • 2008
  • The exploitation of the natural resources of the Moon and other celestial bodies represents one of the most exiting future developments in the field of space law as well as a unique occasion for the economic and social growth of mankind as a whole. The large number of benefits that are expected to be generated from the exploitation of these resources, indeed, not only will contribute to the betterment of conditions of people on Earth but also will allow mankind to face and likely solve one of the biggest problems currently affecting our planet, namely the exhaustion of the stocks of raw materials and other source of energy, such as fossil fuels. The exploitation of the natural resources of the Moon and other celestial bodies, however, has been prevented so far by the absence of dedicated space law rules allowing its orderly and peaceful development and clarifying the rights and duties of the parties involved in it. Due to the uncertainty generated by the absence of these rules, indeed, States as well as private operators have refrained from investing in the exploitation of space resources so far. The time to change this situation and to allow the exploitation of extraterrestrial resources to begin has finally come. This paper aims at fulfilling this purpose by proposing a legal regime containing specific and detailed rules to regulate the exploitation of the natural resources of the Moon and other celestial bodies.

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