• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.395-402
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• 2005

Biologically active recombinant human follicle stimulating hormone (rhFSH) was produced in Chinese hamster ovary cells and purified by a series of chromatographic steps. The chromatographic steps included anion-exchange chromatography (DEAE Sepharose F/F, Q Sepharose F/F), hydrophobic interaction chromatography (Source 15 PHE), and hydroxyapatite chromatography (Macro-Prep ceramic hydroxyapatite type I). A distinctive step of the purification process developed was the use of ZnCl$_2$ for the removal of non-glycosylated or lowly-glycosylated FSH and impurities through co-precipitation with Zn$^{2+}$. Purified rhFSH was identified and characterized by several physicochemical and biological methods such as gel electrophoresis, high-performance liquid chromatography, amino acid analysis, carbohydrate analysis, and biological activity. The overall yield of the purification was ~$30\%$. The rhFSH preparation obtained showed high purity (>$99\%$) and high in vivo potency (>16,000 IU/mg). Carbohydrate analysis suggested that the purified rhFSH contained approximately $40\%$ (w/w) carbohydrate with di­or tri-antennary structure on average, which is somewhat more heavily sialylated than commercially available rhFSH. In conclusion, the results of these analyses established an identity of the purified rhFSH with natural FSH from human pituitary glands, and furthermore, the purified rhFSH preparation showed higher in vivo potency and was slightly more heavily sialylated than commercially available rhFSH.

• Journal of The Korean Society of Agricultural Engineers
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• v.47 no.6
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• pp.83-92
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• 2005

Natural environment of the Wolgokri stream watershed, located in Chuncheon, Gangwon province, Korea, has been well preserved as a traditional agricultural watershed. To analyze characteristics of NPS pollution generated from an mountainous agricultural watershed, the flow and water qualities of the study watershed were monitored and were analyzed to estimate pollution loads. Annual runoff volume ratio was $70.4\%$. Concentrations of T-N, T-p, COD, and TOC were higher when monthly rainfall was between $0\~30mm$ than those when monthly rainfall was between $30\~70mm$. However, the concentrations varied considerably when monthly rainfall was higher than 100mm. The flow weighted mean concentrations(mg/L) of BOD, COD, TOC, $NO_3-N$, T-N, T-P and SS were 1.96, 2.72, 3.32, 1.41, 4.70, 0.187 and 13.36, respectively. The BOD, SS, T-N and T-P loads of July, 2004 were $48\%,\;17\%,\;51\%\;and\;32\%$ of annual load, respectively. The BOD, COD, TOC, $NO_3-N$, T-N, T-p, and SS loads (kg/ha) from Mar. 2004 to Apr. 2005 were 19.09, 26.55, 32.39, 13.85, 45.92, 1.887 and 130.18, respectively. The highest concentrations of BOD, NO3-N, T-N, T-p, SS, COD and TOC were found before the flow reached the peak runoff, possibly due to the first flushing effect. Generally, pollution loads of the Wolgokri watershed were not that significant. Phosphorus load, however, was higher enough to cause eutrophication in the receiving water body It was recommended that best management practices need to be implemented to reduce phosphorus sources.

• Journal of Sensor Science and Technology
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• v.12 no.2
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• pp.88-94
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• 2003

Electromyogram (EMG) is the bioelectric signal induced by motor nerves. Analyzing EMG with the movement produced by muscle contraction, we can provide input commands to a computer as a man-machine interface as well as can evaluate the patient's motional abnormality. In this paper, we developed an integrated miniaturized device which acquires and transmits the surface EMG of an interested muscle. Developed system measures $60{\times}40{\times}25mm$, weighs 100g. Using an amplifier circuitry on the electrodes and the radio frequency transmission, the developed system dispenses with the use of cables among the electrodes, amplifier, and the post processing system (personal computer). The wiring used in conventional systems can be obstacle for natural motion and source of motion artifacts. In results, the developed system improves not only the signal-to-noise ration in dynamic EMG measurement, but also the user convenience. We propose a new human-computer interface as well as a dynamic EMG measurement system as a possible application of the developed system.

• Journal of Life Science
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• v.20 no.6
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• pp.902-906
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• 2010

A producer of thermophilic extracellular lipase, Geobacillus kaustophilus DSM 7263, was selected from various microorganisms of the Geobacillus genus. We investigated optimum conditions for mass production of G. kaustophilus lipase. Among the different natural oil media, olive oil was optimal for enzyme production. The maximum amount of enzyme production was obtained when G. kaustophilus was grown in a medium containing 0.5% olive oil as a carbon source. The pH and temperature for optimal growth were pH 8.0 and $55^{\circ}C$, respectively, while the optimum pH and temperature for lipase production were pH 6.0 and $50^{\circ}C$, respectively. In the presence of $Mg^{2+}$ and $Mn^{2+}$, lipase production was dramatically enhanced by 247% and 157%, respectively, whereas enzyme production was inhibited by $Zn^{2+}$, $Cu^{2+}$, and $Cd^{2+}$. The addition of 0.1% (v/v) triton X-100 increased lipase production and cell growth when compared to the negative control.

• Food Science and Biotechnology
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• v.14 no.1
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• pp.73-76
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• 2005

The acaricidal activities of 12 commercial constituents derived from Eugenia caryophyllata leaf oils against Dermatophagoides farinae, D. pteronyssinus and Tyrophagus putrescentiae adults were examined using an impregnated fabric disk application and compared with that of the commercial benzyl benzoate as synthetic acaricide. On the basis of $LD_{50}$ values, the most toxic compound was methyl eugenol ($4.13\;{\mu}g/cm^2$), followed by methyl isoeugenol ($4.19\;{\mu}g/cm^2$), isoeugenol ($4.29\;{\mu}g/cm^2$), eugenol ($4.94\;{\mu}g/cm^2$), and acetyl eugenol ($13.91\;{\mu}g/cm^2$) against D. farinae. In the case of D. pteronyssinus, isoeugenol ($2.93\;{\mu}g/cm^2$) was the most toxic, followed by methyl isoeugenol ($3.28\;{\mu}g/cm^2$), methyl eugenol ($3.87\;{\mu}g/cm^2$), eugenol ($3.92\;{\mu}g/cm^2$), and acetyl eugenol ($7.21\;{\mu}g/cm^2$). These results suggest that D. pteronyssinus may be controlled more effectively by the application of eugenol congeners than D. farinae. In comparison with synthetic acaricides, the acaricidal activities of eugenol, isoeugenol, methyl eugenol, and methyl isoeugenol were about 1.9-2.2 times more toxic than benzyl benzoate. Furthermore, the most toxic constituent against T. putrescentiae was exhibited on eugenol ($10.11\;{\mu}g/cm^2$), followed by methyl eugenol ($38.67\;{\mu}g/cm^2$) and acetyl eugenol ($70.09\;{\mu}g/cm^2$), but no activity was observed for isoeugenol and methyl isoeugenol. The results suggested that eugenol congeners may be useful as a new source for selective control of house dust mites and stored food mites.

• Lingua Humanitatis
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• v.1 no.1
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• pp.255-274
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• 2001

This paper examines the history of Korean names, focusing on the structural characteristics and the literal meanings of those for kings and ordinary people. Roughly speaking, the Korean surname system traces its source to China. Historical records show that the Chinese were already using their family names around 1000 B.C. After a millennium or so, the Korean began to follow suit. Initially, however, surnames were in regular use among kings and their kin only: it was not until the late Shilla dynasty, around A.D. 800, that the commoners began to have Heir surnames. And yet, surnames still tended to be limited to men of noble birth, until around A.D. 1000. Though the Korean surname system may be said to have its origin in Chinese, there are no lack of native surnames that have as long a history, e.g., Hae, Bak, Sok, Sol, Ul, and Ol. Typical surnames of Chinese origin that are found during the late Shilla dynasty, around A.D. 900, include Jang (as in Jang Bogo) and Choi (as in Choi Chiwon). Unlike those of Chinese origin, typical Korean names during the age of Three Nations tended to allude to nature or natural phenomena, as can be witnessed in Bit 'light,' Balgum 'bright,' Nuri 'world,' and Soidori where soi means 'steel' and dol 'stone,' Other surnames abound that have to do with river, stream, field, prairie, and so on. King Muryong's original name was Sama, a variant of som 'island,' indicating where he was born. A person skilled in archery was called Jumong or Hwalbo. Baembo was so called because he 'crawls like a snake, baem,' In these last two examples, bo, as well as its variant bu, functions as a suffix, and is also found in Cochilbu, Isabu, Babo, Nolbu, Hungbu, etc. The tradition of using this kind of native Korean names has gradually disappeared, giving place to the names of Chinese style-although very recently we see an on-going movement toward using native names.

• Food Science and Preservation
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• v.19 no.5
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• pp.735-743
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• 2012

Purple sweet potato (PSP, Ipomoea batatas) has various biological effects including antidiabetic, anti-inflammatory, and antioxidant activities. In this study, the antioxidant activity of PSP after roasting were compared using DPPH, ABTS, and FRAP assays. In addition, the total phenolics and flavonoid contents, Maillard reaction products, and phenolic acid contents were measured to identify the factors that changed PSP,s antioxidant activity due to roasting. The roasted PSP exhibited significantly higher antioxidant activity than unroasted PSP. In particular, the PSP roasted at $200^{\circ}C$ for 10 min showed the highest antioxidant activity among all the PSPs that were roasted under different conditions. The total phenolic and flavonoid contents, Maillard reaction products and phenolic acid contents markedly increased, corresponding to the general increase in antioxidant activities after roasting. These results suggest that roasted PSP extracts are potential source of natural antioxidants that may be used in certain food applications.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11a
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• pp.20-33
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• 2001

A major portion of drugs used in Ayurvedic system of medicine which has been practiced since the early human civilization in Indian subcontinent were of plant origin. It should be noted that 70% of the population in this region depends on Ayurveda for their medical treatment and 60% of the drug resources are obtained from the Himalayan region. Therefore, Nepal becomes a potential source of plant drug resource since it occupies a major portion of the Himalaya. In the present paper, in general a current status of medicinal plant resources of Himalayan region especially Nepal will be discussed. In addition to this, a typical example of antidiabetic activity of Shilajit will be taken for the discussion. Shilajit is one of the crucial elements in several formulations including those of Rasayana, a therapy in Ayurveda, which has been practiced in the prevention of ageing and mental disorder. Although, Shilaiit is widely used for the treatment of diabetes, no satisfactory scientific reports are available up to now. The crude Shilajit in the market is a dark brown or black rock-like substance collected from the Himalayan region with a strong smell of cow's stale urine. In our studies, Shilajit (collected in the central Himalayan region) prevented the diabetes in nonobese diabetic (NOD) mice model. Shilajit also prevented the diabetes in the rats against the action of multiple low-dose (10 ㎎/㎏, i.v., 5 times) of streptozotocin. On the other hand, Shilajit did not show antioxidative activity. The preventive action of Shilajit on diabetes is mainly focused on the Th1 and Th2 cell activities, since Th2 cells activity was found to be significantly upregulated. Shilajit, however, showed a mild action in controlling the blood sugar level in young, old, and mild diabetic rats, but not in the severe diabetic rats. It also stimulated the nitric oxide production in macrophages. Based on these evidences, the antidiabetic activities of Shilajit appear to be immunomodulative probably by protecting or strengthening insulin-producing b-cells In the pancreas. further systematic research on constituents of Shilajit and its quality evaluation is necessary to enable the use of natural medicines in the treatment of diabetes.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.1 no.1
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• pp.22-30
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• 2020

Many areas of vegetation in the British uplands have reduced species diversity as a result of sheep overgrazing. It has been suggested that abandonment or re-wilding strategies might be used to reverse this. A likely first step would be the removal or reduction of grazing livestock from upland areas, with a presumption that this would lead to a recovery in species richness. However, we do not know if this would work, or the timescales involved. One of the important areas where more knowledge is needed is information on the size and composition of soil seedbanks as regeneration from zseed is a likely pathway of recovery. Here, we compared seedbanks in both grazed and ungrazed plots in five experiments at Moor House NNR in the northern Pennines; these sheep grazing exclusion experiments were started 52 and 63/64 years ago. Soil samples (n=10) were collected from both grazed and ungrazed plots in each experiment, and seed emergence counted in glasshouse trials. We detected only seeds of common species and very few dicotyledonous species. This suggests that the soil seedbank is unlikely to be a reliable source of the less common species for ecological restoration in these upland communities, suggesting an extinction debt. Therefore, seed addition and the creation of suitable safe-sites for germination may be needed in conjunction with grazing controls to allow the establishment of plants that will increase the species richness of the vegetation. However, this interventionist restoration approach remains to be tested.

• Journal of Life Science
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• v.24 no.8
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• pp.903-909
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• 2014

Plants are an invaluable source of potential new anti-cancer drugs. Sasa quelpaertensis Nakai (Korean name, Jeju-Joritdae) is one of these plants with medical value, which is a bamboo grass widely distributed in Mt. Halla on Jeju Island, Korea. Here, we investigated the apoptotic effects of S. quelpaertensis leaf extracts in six human cancer cell lines (A549, MCF-7, HepG-2, Hela, HCT116 and A375). MTT assay signified the antiproliferative nature of S. quelpaertensis extracts against all tested cancer cells: S. quelpaertensis displayed slight cytotoxicity against A549, MCF-7 and HepG-2 cells, whereas it was exclusively cytotoxic to Hela, HCT116 and A375 cells. Apoptotic cells were evaluated using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). PI staining indicated increasing accumulation of Hela, HCT116 and A375 cells at sub-G1 phase. Further events like generation of nitric oxide ($NO^{\bullet}$) were accompanied in the S. quelpaertensis Nakai-induced apoptosis. Augmented $NO^{\bullet}$ generation resulted in the DNA fragmentation of Hela, HCT116 and A375 cells by treatment with S. quelpaertensis leaf extracts. These results suggest that S. quelpaertensis may be a potential natural resource for treating cancer cell. To identify the exact mechanisms of molecular mechanism of S. quelpaertensis induced apoptosis awaits further investigation.