• Parasites, Hosts and Diseases
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• v.52 no.3
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• pp.305-310
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• 2014

Ascidian soft tunic syndrome (AsSTS) caused by Azumiobodo hoyamushi (A. hoyamushi) is a serious aquaculture problem that results in mass mortality of ascidians. Accordingly, the early and accurate detection of A. hoyamushi would contribute substantially to disease management and prevention of transmission. Recently, the loop-mediated isothermal amplification (LAMP) method was adopted for clinical diagnosis of a range of infectious diseases. Here, the authors describe a rapid and efficient LAMP-based method targeting the 18S rDNA gene for detection of A. hoyamushi using ascidian DNA for the diagnosis of AsSTS. A. hoyamushi LAMP assay amplified the DNA of 0.01 parasites per reaction and detected A. hoyamushi in 10 ng of ascidian DNA. To validate A. hoyamushi 18S rDNA LAMP assays, AsSTS-suspected and non-diseased ascidians were examined by microscopy, PCR, and by using the LAMP assay. When PCR was used as a gold standard, the LAMP assay showed good agreement in terms of sensitivity, positive predictive value (PPV), and negative predictive value (NPV). In the present study, a LAMP assay based on directly heat-treated samples was found to be as efficient as DNA extraction using a commercial kit for detecting A. hoyamushi. Taken together, this study shows the devised A. hoyamushi LAMP assay could be used to diagnose AsSTS in a straightforward, sensitive, and specific manner, that it could be used for forecasting, surveillance, and quarantine of AsSTS.

• Applied Biological Chemistry
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• v.39 no.2
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• pp.147-152
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• 1996

To screen inhibitors on complement system from natural resources, micro-screening method was established by using hemolytic complement assay. Complement fixation reaction was carried out in the microplate system. For standard hemolysis (50% hemolysis) of the classical pathway (CP), hemolysin and complement serum were diluted to $1/75{\sim}1/100\;and\;1/80{\sim}1/120$, respectively, when sheep erythrocytes were $5.0{\times}10^8\;cells/ml$. In case of the alternative pathway (AP), complement serum was diluted to 1/5 and EGTA and $Mg^{2+}$ were added 4 mM, $4{\sim}8\;mM$, respectively, when rabbit erythrocytes were $4.0{\times}10^8\;cells/ml$. Dimethyl sulfoxide was used for the assay of non-aquous soluble compounds or extracts and its final concentration was not more than 1%. Three phenylpropanoids showed anticomplementary activities in proportion to the concentration for both pathways and rosmarinic acid exihibited the highest inhibitory activities: $5.4{\pm}3.6%(0.063\;mM){\sim}95.8{\pm}0.2%(0.5\;mM)\;and\;35.1{\pm}0.9%(0.063\;mM){\sim}95.6{\pm}1.1%(1\;mM)$ on the CP and the AP, respectively.

• The Journal of Natural Sciences
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• v.8 no.2
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• pp.289-321
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• 1996

After the two big wars (Imjin Woeran and Byungia Horan), the Lee Dynasty confronted rapid transitional periods in almost all aspects of the society. Corruption of the governmental system by the wars and disordered social structures also caused confusion in the social position system. In addition, development of the commercial economic system and monetary economy brought up a new rich middle-class, "Joong in Gyegeup". In ideological aspects, Confucianism indulged in and stick to isms and ics, and the Confucian morality slackened, thus a more pragmatic ideology, "Silhak Sasang", developed. And the emergence of the modern literature and art of the 'common people' was followed by the appearance of the common-people class including those having the common-people concept among the noble-class "Yangban Gyegeup". This evoked a new common costume culture and the fashion appeared. The public could no longer find the power and appeal from the preexisting ruling classes due to the changed role and sense of value. Thus, they sought a new symbolic leader group that would to be the new standard of the newly changed society. That was a group of people called as "Kisaeng" liberal and independent. They were performing social activities, enjoying poetry, music and dancing, and classy dressers, having physically attractive bodies. The erotic as well as luxurious mode proposed by them reflected the trend of that time. It concurred well with the concept and taste of the public, so it was accepted with a good response. That is, by following these leaders, the public achieved their identification and tried to share the prestige of the leaders. For this reason, the Kisaeng group attracted the public attention and led the taste of the public, thereby becoming the influential fashion leaders of that age.

• Journal of the East Asian Society of Dietary Life
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• v.21 no.3
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• pp.375-384
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• 2011

The effects of mixed beans on quality characteristics of traditional soybean paste (Doenjang) were investigated. The proximate composition of the soybean paste (Doenjang) met traditional food standard requirements. The range in salinity was 12.30~13.20%, and the salinity decreased with an increase in the amount of mixed beans. A significant difference in pH values occurred in all samples(p<0.05). The Hunter's 'L ($45.06{\pm}0.41$)' and 'b ($13.89{\pm}0.73$)' values of the TDM2 samples were higher than those of other samples. The highest amounts of malic acid of the organic acids are shown. The order of the amino acid content was aspartic acid > leucine > lysine, and that of free amino acid contents were proline > glutamic acid > arginine. The order of mineral content in the soybean pastes was Na > K > Ca > Mg, but levels of Co, Cu, and Zn were not detected. The traditional soybean paste (Doenjang) had an effective DPPH radical scavenging activity and higher phenolic content compared to those of the control sample. Overall acceptability score of the TDM2 (soybean 1 : mixed beans 1) was higher than that of the others. More research is needed to enhance the quality and functionality of traditional soybean paste.

• Journal of Digital Convergence
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• v.18 no.8
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• pp.311-319
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• 2020

The purpose of this study was to investigate the effects of mineral taping containing natural minerals powder on knee joint pain and swelling in the elderly. Forty elderly people with knee pain were divided into 20 mineral taping groups and 20 kinesio taping groups and randomly placed in a single blind manner. The study period was conducted from April 6, 2020 to April 7, 2020, and pain and blood flow by time point were examined before, after, and after 1 day of taping. Knee pain was significantly different in the post-test (F = 93.758, p <.001) and the post-day test (F = 93.758, p <0.001) for each measurement point. The blood flow test for edema was significantly different in the post-test (F = 48.648, p <0.001) and the post-day test (F = 35,427, p <0.001) for each measurement time point. There was no significant difference in the effect test result for each group (F = 0.160, p> 0.05). However, When comparing the retention effect after 1 day, the mineral taping group was excellent to the post-score than the Kinesio taping group. In the future, we believe that increasing the taping time and retention period will have a positive effect. It is thought that the results of this study will contribute to the study of taping materials in the future.

• Journal of Environmental Science International
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• v.20 no.11
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• pp.1447-1455
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• 2011

The purpose of this study was to analyze physicochmical characteristics of oil within planting base of Incheon coastal reclamation cities where 10 years have passed since reclamation, and to provide basic data to promote growth and development of plants by conducting evaluation based on the planting degree standard. Study targets were Cheongra, Sondo, and Yeongjong districts within Incheon Free Economic Zone, the coastal reclamation city. The analysis results of average soil characteristics of 3 districts. Soil acidity(pH) was shown in orders of Cheongra district(8.85) > Songdo district(8.70) > Yeongjong district(7.97) and electric conductivity(EC) was shown in orders of Cheongra district(4.80 dS/m) > Songdo district(1.30dS/m) > and Yeongjong district(0.07 dS/m). Organic matter content(OMC) was shown in orders of Cheongra district(5.72%) > Songdo district(2.60%) > Yeongjong district(0.59%) and available phosphate was shown in orders of Cheongra district(70.70 mg/kg) > Songdo district(23.07 mg/kg) > Yeongjong district(2.49 mg/kg). $K^+$ was shown in orders of Songdo district(0.84 cmol/kg) > Cheongra district(0.74 cmol/kg) > Yeongjong district(0.22 cmol/kg), $Ca^{++}$ was shown in orders of Cheongra district(22.08 cmol/kg) > Songdo district(9.87 cmol/kg) > Yeongjong district(4.04 cmol/kg), and $Mg^{++}$ was shown in orders of Cheongra district(1.98 cmol/kg) > Songdo district(1.22 cmol/kg) > Yeongjong district(1.12 cmol/kg). Planting base level of coastal reclaimed land can be applied with above intermediate level, soil acidity and available phosphate were all more than intermediate in 3 districts, and electric conductivity was low level in Cheongra district and more than intermediate level in Songdo and Yeongjong districts. Organic matters content was more than intermediate level in Cheongra district and low level in Songdo and Yeongjong district. $K^+$ among exchangeable ions was intermediate level in Cheongra and Songdo districut and low in Yeongjong district. $Ca^{++}$ and $Mg^{++}$ both were more than imtermidiate levels in 3 districts.

• Journal of the Korean Geotechnical Society
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• v.27 no.7
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• pp.17-33
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• 2011

Recently, the utilization of recycled aggregates for backfilling a power transmission pipeline trench has been considered due to the issues of eco-friendly construction and a lack of natural aggregate resource. It is important to identify the physical and thermal properties of domestic recycled aggregates that can be used as a backfill material. This paper evaluated thermal properties of concrete-based recycled aggregates with various particle size distributions. The thermal properties of the recycled aggregates and river sand provided by local vendors were measured using the transient hot wire method and the transient needle probe method after performing the standard compaction test. The needle probe method considerably overestimated the thermal resistivity of recycled aggregates especially at the dry of optimum water content because of experiencing disturbance while the needle probe is being inserted into the specimen. Similar to silica sand, the thermal resistivity of recycled aggregates decreased when the water content increased at a given dry density. Also, this paper evaluated some of the existing prediction models for the thermal resistivity of recycled aggregates with the experimental data, and developed a new prediction model for recycled aggregates. This study shows that recycled aggregates can be a promising backfill material substituting for natural aggregates when backfilling the power transmission pipeline trench.

• Korean Journal of Environment and Ecology
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• v.12 no.1
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• pp.78-90
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• 1998

An objective of this study was to provide database for the planting disign of buffer green space. Types, planting structure, and effect of vuffer green space were investigated in five housing complexes of newtown of metropolitan area, Korea. Buffer green space in the study sites were constructed as mounding, slope, and plate. The number of species was found 20 tree and sub-tree species(10 evergreen and 20 deciduous species ) and 13 shrub species. These species were planted in one-storyed planting structure and there was no difference with ornamental species in the urban parks. Effect of sound proof by the buffer green space was recognized but sound level in four types among the seven types was observed above standard sound level for housing complex(65dB). Effect of sound proof was especially most effective in the mounding type. It was found that planting density and index of plant crown volume were mot satisfied to the function of buffer green space because of lower density and crown volume than natural vegetation per unit. Based on these results, this study suggested that buffer green space is desirable to be developed in the mounding type over two meters height with multi-layer planting model. In addition, there is needed to consider vegetation structure of natural forest around the developing site.

• Korean Journal of Environment and Ecology
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• v.27 no.4
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• pp.429-436
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• 2013

Churyeong Stream flows 37km from the mouth of Lake Okjeong and is impeded by many weirs that hamper the active movement of land-locked sweet smelt fish (Plecoglossus altivelis) that ascend and descend between the lake and the upper stream. In late December 2006, a fishway was constructed in a weir of the lower reach of Churyeong Stream, where juveniles begin their ascent to mature upstream. At 13 sites along the stream from April to October 2007, we investigated the effects of the newly constructed fishway on sweet smelt growth. After construction of the fishway, juvenile sweet smelt were able to migrate freely upstream, something that had previously only been possible when the weir was in flood. The body size of mature sweet smelt collected during the spawning season in September 2007 was greatly increased compared to previous seasons, measuring an average of $163{\pm}21.5mm$ in standard length. These measurements were 13mm, 20mm, and 57mm longer than body length averages for mature sweet smelt collected in 2006, 2004, and 2005, respectively. Therefore, it is likely to be necessary for the construction of fishways on streams and rivers containing land-locked sweet smelt populations to increase average body sizes and viability.

• The Korean Journal of Zoology
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• v.27 no.2
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• pp.103-116
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• 1984

The structural gene of rabbit hemoglobin was cloned into Pst I site of pBR322 in E. coli. The complementary DNA (cDNA) was synthesized from rabbit globin mRNA with avian myeloblastosis viral reverse transcriptase, and then RNA was destroyed at pH 11. The double stranded cDNA was synthesized with both Klenow fragment of E. coli DNA polymerase I and reverse transcriptase and then the hairpin loop was opened with Sl nuclease. Double stranded cDNA was subsequently tailed with dCTP and annealed to dGMP-tailed vector DNA. After transformation and initial screening of appropriate clones by plasmid size, the cloned colonies were identified by in situ colony hybridization using by plasmid size, the cloned colonies were identified by in situ colony hybridization using $[^32P]$-labeled cDNA probes and characterized the inserts with restriction endonucleases. The expression of cloned globin gene was investigated by standard radioimmunoassay using rat anti-rabbit Hb serum as primary antibody and goat antirat IgG serum as secondary antibody. The result suggested that the chimeric proteins (the part of $\\beta$-lactamase from the vector pBR322 and globin from rabbit) were supposedly produced in E. coli and the product had the antigenic determinant of rabbit hemoglobin.