• Title/Summary/Keyword: Natural Extract

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Screening of Natural Antimicrobial Plant Extract on Food Spoilage Microorganisms (식품 부패미생물의 증식을 억제하는 천연 항균성물질의 검색)

  • Lee, Byung-Wan;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.23 no.2
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    • pp.200-204
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    • 1991
  • Certain parts of 36 kinds of plant were extracted by 75% ethanol and water. The extracts were tested their microbial inhibition activities against several food spoilage microorganisms, Bacillus subtilis, Lactobacillus plantarum, Leuconostoc mesenteroides, Pseudomonas fluorescens, Bacillus cereus and Saccharomyces cerevisiae. The ethanol extract of amur cork was shown inhibitory effect on all 6 species of the microorganisms tested. Chinese pepper, sesame cake, gromwell and oak were on 5 species except S. cerevisiae or P. fluorescens and bamboo leaves, lycopi herba, paulownia and rigida were on 4 species. In general amur cork exhibited the strongest inhibition with a few exceptions on certain species. By disc diffusion method, the ethanol extract of leaf mustard showed the highest inhibition effect on B. subtilis, amur cork on L.plantarum, L. mesenteroides and B. cereus, and amur cork and gallnut on P. fluorescens. Mostly the ethanol extracts in comparison with water extracts showed higher inhibition in most of plants but a few exhibited higher in water extracts.

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The Dyeability of Silk Fabrics with Sweet Potato Stem·Leaf Extract (고구마 줄기·잎 추출액을 이용한 견직물의 염색성)

  • Hong, Bo Geun;Lee, Jeong Sook
    • Textile Coloration and Finishing
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    • v.28 no.3
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    • pp.219-229
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    • 2016
  • The purpose of this study was to investigate the dyeability of silk fabrics with sweet potato stem leaf extract. To obtain the optimal dyeing conditions it was examined at various dyeing conditions(temperature, pH, time and bath ratio). The dyeability and the depths of shade which were evaluated in terms of K/S and CIELAB color difference values of the dyed and mordanted fabrics were also investigated. After dyeing, various color fastness(wash fastness, dry cleaning fastness, light fastness, rub fastness, and perspiration fastness) was measured and reviewed for UV protective, deodorant and antimicrobial functionality. The optimun output, as a result, was shown at 100% of dye concentration, $90^{\circ}C$ of dyeing temperature and 80 minutes of dyeing time while in terms of dye uptake depending on the kind of mordants and mordanting, it was found that among four mordants of $Alk(SO_4)_2$, $CuSO_4$, $SnCl_2$, and $FeSO_4$, post-mordanting with $SnCl_2$ showed the best results. Color fastness to dry cleaning, washing and rubbing was found strong at grade 4-5 and the grade to perspiration was as good as 3 while to light fastness was good at 4 as well. In aspects of functional properties, it showed excellent results of 98.3% UV protection rate and 88% deodorization rate. Antibacterial activity was 99.9% against staphylococcus aureus and 73.3% against klebisella pneumoniae. In conclusion, we validated that the dyestuffs from the disused sweet potato stem leaf extract would be useful as a natural dye material using the optimized conditions and dyeability for silk dyeing.

Dyeing of Wool Fabric by the Pigment Extracted from Opuntia Ficus-indica (선인장 열매의 색소 추출물에 의한 양모섬유의 염색)

  • Lee Se-Hee;Cho Yong-Suk;Choi Soon-Hwa
    • Textile Coloration and Finishing
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    • v.18 no.2 s.87
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    • pp.8-14
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    • 2006
  • The pigment extraction of Opuntia ficus-indica has been conducted to develop useful natural dyes in place of synthetic dyes which are suspected to bring serious environmental pollutions. The dyeing ability on wool fabric by addition of ascorbic acid and several mordants were investigated by means of color measurement. In addition, the fastness of washing, perspiration, rubbing, light, dry cleaning, effect on bacterial reduction and UV-B protection were also investigated. From these investigation, it is suggested that the pigment extracted from Opuntia ficus-indica can be used as a source of natural dyes and the obtained result are as follows. 1. Maximum absorption band (${\lambda}max$) of Opuntia ficus-indica extract is 533nm. 2. The wool fabric dyed with Opuntia ficus-indica extract has stable color by the addition of ascorbic acid and is achieved with addition of 0.1% ascorbic acid, 0.5% several mordant, and three repeated dying at $50^{\circ}C$ for 1.5hr. 3. The wash fastness of the dyed wool fabric when it is washed with neutral detergent is more effective than alkaline detergent. The dry cleaning fastness of the dyed wool fabric is more excellent. In addition, the perspiration fastness of the dyed wool fabric is increased by mordanting method. And than the rubbing fastness of the dyed wool fabric is showed excellent under dryness and wetness. Light fastness of the dyed wool fabric, however is showed inferiority. 4. The wool fabric dyed with Opuntia ficus-indica extract is showed effective bacterial reduction and UV-B protection is increased remarkably.

Effects of Anthriscus sylvestris Hoffmann Extract on the Biosynthesis of Collagen in Human Dermal Fibroblasts (사람 섬유아세포에서 전호(Anthriscus sylvestris Hoffmann)추출물이 콜라겐 생합성에 미치는 영향)

  • Lee, Woo-Jung;Kim, Yong-Kee;Kim, Su-Nam
    • Korean Journal of Plant Resources
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    • v.25 no.2
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    • pp.240-245
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    • 2012
  • In this study, we investigated the boosting effects on collagen biosynthesis of $Anthriscus$ $sylvestris$ ethanol extract (ASE) in human dermal fibroblasts. To obtain more effective fraction and subfraction for collagen biosynthesis, standard solvent partition and open column chromatography were performed. The EtOH extract, solvent fractions, and 8 EtOAc subfractions were tested for their collagen synthesis capacity by [$^3H$]Proline-incorporation and ELISA assay. ASE increased 25% of total collagen synthesis and 27% of procollagen biosynthesis. The total collagen biosynthesis was increased by EtOAc fraction and E6 subfraction to 28% and 50% respectively. Type I procollagens were also upregulated by EtOAc fraction and E6 subfraction to 30% and 47%, each. Taken together, our data suggest that potential anti-aging effect of ASE on skin is via increasing collagen biosynthesis and effective subtraction is E6 subfraction of EtOAc fraction.

Screening of Natural Antioxidant from Plant and Their Antioxidative Effect (식물성 천연 항산화물질의 검색과 그 항산화력 비교)

  • Choi, Ung;Shin, Dong-Hwa;Chang, Young-Sang;Shin, Jae-Ik
    • Korean Journal of Food Science and Technology
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    • v.24 no.2
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    • pp.142-148
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    • 1992
  • Certain parts of 95 species of edible and medical plants were extracted with water and 75% of ethyl alcohol. After addition of those extracts to palm oil, lard and soybean oil at different level, their antioxidative activities were compared by Rancimat test. Six species among them seemed to have rather strong antioxidative activity and high extracting yields(i.e. Taraxacum platycarpum, Plantago asiatica, Rhus javanica L., Lycopus lucidus, Astragalus membranaceus, Taraxacum platycarpumH). Among them, the Rhus javanica L. ethanol extract retarded greatly the induction period of palm oil and lard. When 600 ppm of Rhus javanica L. extract were added to palm oil and lard, AI(antioxidant index was expressed as induction period of oil containing various plant extracts/induction period of control oil) of each was 1.35 and 3.03 respectively. This result indicated that the Rhus javanica L. extract was more effective on lard than the other oils.

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Antimicrobial Activity of Water-soluble Extract from Artemisia princeps var. orientalis (Artemisia princeps var. orientalis 수용성 추출물의 항균효과)

  • Cho, Hwa-Young;Yoon, Sung-Yong;Park, Jeong-Jin;Yun, Kung-Won;Park, Jong-Moon
    • KSBB Journal
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    • v.21 no.2
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    • pp.129-132
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    • 2006
  • The importance of natural preservative has increased in recent years. The natural preservatives have been used in the field of foods, cosmetics and pharmacology. In the present work Artemisia sp., well recognized for their effect of antimicrobial activity, were extracted by methanol and water sequentially for selecting only water-soluble compounds that can be used as additives in food and cosmetics. Antimicrobial activities of water extracts from stem and leaf of Artemisia princeps var. orientalis were investigated by the disc diffusion method. Two gram positive bacteria(Staphylococcus aureus and Bacillus subtilis) and three gram negative bacteria(Escherichia coil, Agrobacterium tumefaciens and Pseudomonas putida) were used for antimicrobial activity studies. The water-soluble compounds from methanol extract showed higher antimicrobial activity than only water extract to these bacteria. Comparative evaluation of water-soluble metabolite profiles with caffeic acid that is known as an antimicrobial compound from Artemisia sp. was performed by high performance liquid chromatography with photo-diode array detection.

Combined Treatment of Acorn Pomace Extract, Fumaric Acid, and Mild Heat for Inactivation of Microorganisms on Red Chard (도토리박 추출물과 푸마르산 및 중온 열 병합처리에 의한 적근대의 미생물 제어 효과)

  • Park, Shin-Min;Son, Kyung Bin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1696-1700
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    • 2016
  • In this study, acorn pomace extract (APE) was developed as a natural chemical sanitizer and substitute for chlorine-based sanitizers such as sodium hypochlorite containing harmful substances. Antimicrobial activities of APE and its combined treatments with fumaric acid (FA) and mild heat against Listeria monocytogenes inoculated on red chard were examined. Among the treatments, combined treatment of 0.5% APE at $50^{\circ}C$ and 0.5% FA was the most effective, causing reduction of L. monocytogenes populations by 3.36 log CFU/g compared to the control. After combined treatment, populations of aerobic mesophilic bacteria in the red chard decreased by 2.89 log CFU/g during storage at $4^{\circ}C$ for 8 days compared to the control. Regarding color changes in red chard upon combined treatment, there was no significant change among the red chard samples. These results indicate that combined treatment of APE, FA, and mild heat can improve microbial safety of red chard without affecting quality such as color during storage.

Inhibition of Apoptosis by Elaeocarpus sylvestris in Mice Following Whole-body Exposure to Ionizing Radiation: Implications for Radioprotectors

  • Park, Eun-Jin;Lee, Nam-Ho;Ahn, Gin-Nae;Baik, Jong-Seok;Lee, Je-Hee;Hwang, Kyu-Kye;Park, Jae-Woo;Jee, Young-Heun
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.718-722
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    • 2008
  • Elaeocarpus sylvestris var. ellipticus (E.S.), which contains 1, 2, 3, 4, 6-penta-O-galloyl-beta-D-glucose (PGG), is reported to have the ability to scavenge oxygen radicals, thereby protecting rat neuronal cells from oxidative damage. The potential of an E.S. extract, which contains a rich PGG, to protect radiosensitive lymphocytes and intestinal crypt cells from radiation injury induced by a single whole-body irradiation (WBI) in vivo was investigated. Our results demonstrated that in immune cells, E.S. treatment decreased the percent of tail DNA, a parameter of DNA damage, compared with levels in untreated, irradiated controls. Furthermore, apoptosis was significantly decreased in lymphocytes and intestinal crypt cells of E.S.-treated mice compared with irradiated controls. These results suggest that the E.S. extract can strengthen the radioresistance of radiosensitive lymphocytes and crypt cells by preventing apoptosis. Therefore, it was concluded that E.S. extract has the radioprotective effects in vivo through an inhibition of apoptosis.

Inhibitory Effects of Methanolic Extracts of Medicinal Plants on Nitric Oxide Production in Activated Macrophage RAW 264.7 Cells (약용식물 추출물에 의한 면역세포 산화질소 생성 억제 활성 분석)

  • Seo, Jin-Suk;Lee, Tae-Hoon;Lee, Sang-Min;Lee, Seung-Eun;Seong, Nak-Sul;Kim, Ji-Young
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.3
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    • pp.173-178
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    • 2009
  • A variety of herbs and plants have been traditionally used in oriental folk medicine for the treatment of inflammatory diseases. In our attempt to search for anti-inflammatory agents from natural products, we investigated 64 methanol extracts from 42 medicinal plants belonging to 10 families which were evaluated for inhibitory activities of NO production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Among them, 16 extracts exhibited inhibitory activities of NO production ($IC_{50}$ values ranging from 59.6 to 94.7 ${\mu}g/m{\ell}$). Only the extract from aerial parts of Hosta lancifolia (H. lancifolia) did not exert cytotoxic effects at the concentrations tested. The extract from H. lancifolia decreased the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in activated macrophage RAW 264.7 cells in dose-dependent manner. The results suggest that the extract may contain bioactive compounds that suppress expression of pro-inflammatory cytokines, which may prove beneficial with regard to the development of natural agents for prevention and treatment of inflammatory diseases.

Anti-inflammatory effect of Distylium racemosum leaf biorenovate extract in LPS-stimulated RAW 264.7 macrophages cells (LPS로 유도된 RAW 264.7 세포에 대한 조록나무 잎 Biorenovation 추출물의 항염증 활성)

  • Hong, Hyehyun;Lee, Kyung-Mi;Park, Taejin;Chi, Won-Jae;Kim, Seung-Young
    • Journal of Applied Biological Chemistry
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    • v.64 no.4
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    • pp.375-382
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    • 2021
  • Biorenovation is a microbial enzyme-based structural modification of component compounds in natural products and synthetic compounds including plant extracts with the potential benefits of improved biological activities compared with its reaction substrates. In this study, we investigated the anti-inflammatory activity of Distylium racemosum leaf extract and D. racemosum leaf biorenovation extract (DLB). As a result, DLB inhibited nitric oxide, prostaglandin E2, and inflammatory cytokines including tumor necrosis factor-α, interleukin-6, interleukin-1β at non-toxic concentrations. In addition, DLB significantly inhibited inducible nitric oxide synthase and cyclooxygenase-2 on LPS-treated RAW 264.7 macrophages. Based on these results, we suggest that the DLB could be used as a potent anti-inflammatory agents. It also suggests that the application of biological evolution has potential usefulness to increase the practical value of natural products.