• Journal of Ecology and Environment
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• 제38권4호
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• pp.461-476
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• 2015

Global warming has accelerated glacial retreat in the high Arctic. The exposed glacier foreland is an ideal place to study chronosequential changes in ecosystems. Although vegetation succession in the glacier forelands has been studied intensively, little is known about the microbial community structure in these environments. Therefore, this study focused on how glacial retreat influences the bacterial community structure and its relationship with soil properties. This study was conducted in the foreland of the Midtre Lovénbreen glacier in Svalbard (78.9°N). Seven soil samples of different ages were collected and analyzed for moisture content, pH, soil organic carbon and total nitrogen contents, and soil organic matter fractionation. In addition, the structure of the bacterial community was determined via pyrosequencing analysis of 16S rRNA genes. The physical and chemical properties of soil varied significantly along the distance from the glacier; with increasing distance, more amounts of clay and soil organic carbon contents were observed. In addition, Cyanobacteria, Firmicutes, and Actinobacteria were dominant in soil samples taken close to the glacier, whereas Acidobacteria were abundant further away from the glacier. Diversity indices indicated that the bacterial community changed from homogeneous to heterogeneous structure along the glacier chronosequence/distance from the glacier. Although the bacterial community structure differed on basis of the presence or absence of plants, the soil properties varied depending on soil age. These findings suggest that bacterial succession occurs over time in glacier forelands but on a timescale that is different from that of soil development.

• 대한약침학회지
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• 제22권4호
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• pp.269-278
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• 2019

Objectives: Naesohwangryeon-tang (NHT) is a type of traditional herbal formula, however, little is known about its antitumor activity. In this study, the antitumor properties of NHT was evaluated in human lung adenocarcinoma cells. Methods: To check the inhibitory effect of NHT, MTT assay was performed. Cell cycle analysis and detection of ROS production were conducted by flow cytometry. To evaluate the signaling pathway, Western blotting was conducted. Results: Our results showed that the decrease of cell proliferation by NHT stimulation occurred more significantly in A549 cells than in NCI-H460 cells. In addition, NHT-induced apoptosis was associated with the activation of caspases and production of reactive oxygen species (ROS). NHT-induced apoptosis was attenuated after pretreatments with z-VAD-fmk or N-acetylcysteine, suggesting that NHT-induced apoptosis was caspaseand ROS-dependent. Interestingly, NHT treatment led to the development of autophagic vesicular organelles and upregulation of several autophagy-related genes. The pretreatment of bafilomycin A1 decreased apoptosis slightly but increased cell viability in the presence of NHT. Conclusion: These findings indicated that NHT induces both apoptosis and cell-protective autophagy in human lung cancer cells. This data suggests that NHT might be a novel herbal drug for lung cancer.

• Journal of Ecology and Environment
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• 제36권1호
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• pp.39-47
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• 2013

The expression of transgenic traits in genetically modified crops is sometimes associated with decreases in crop performance or fitness. These decreases in performance or fitness of transgenic plants in unfavourable conditions may provide valuable information about the ecological consequences of transgene escape. In a glasshouse trial, we tested the cost associated with resistance to herbicides by comparing the growth, yield, and competitive ability of transgenic rice with its parental non-transgenic line. This new line was developed for constitutive overexpression of protoporphyrinogen oxidase (PPO) to increase resistance to herbicides. We evaluated nine agronomic traits of transgenic and non-transgenic rice grown in a replacement series design over four densities. Competitive ability was also assessed between transgenic and non-transgenic plants by analyzing their relative yields based on biomass and seed weight data. Our results indicated that non-transgenic plants showed greater performance than did the transgenic plants when those genotypes were grown in mixtures. The non-transgenic rice plants exhibited superior competitive ability at certain combinations of planting densities and genotype proportions. These results suggest that PPO-herbicide resistance incurs some costs in plant performance and competitive ability.

• 한국조경학회지
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• 제33권4호
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• pp.97-107
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• 2005

This study was carried out to construct accurate and scientific system of assessment indicators in selection of National Trust conservation areas, which was new concept of domestic environment movement and offer the raw data of new analytic method by introducing the fuzzy theory and weight for overcoming the uncertainty of ranking decision. To transform the Likert's scale granted to assessment indicators into the type of triangular fuzzy number(a, b, c), there was conversion to each minimum(a), median(b), and maximum(c) in applying membership function, and in using the center of gravity and eigenvalue, there was to decide the ranking. The rankings of converted values applied a mean importance and weight were confirmed that they were generally changed. Therefore, the ranking decision was better to accomplish objective and rational ranking decision by applying weight that was calculated in grouping of indicator than to judge the singular concept and to be useful in assessment of diverse National Trust site. In the future, because AHP, which was general method of calculating weight, was lacked, there was to understand the critical point to fix a pertinent weight, and to carry out the study applying engineering concept like fuzzy integral using $\lambda-measure$.

• Journal of Dairy Science and Biotechnology
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• 제36권1호
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• pp.1-13
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• 2018

Kefir exhibits antimicrobial activity in vitro against gram-positive and gram negative bacteria, as well as some fungi. The ability of LAB to inhibit the growth of closely related bacteria is well known. This inhibition of pathogenic and spoilage microbes may be due to the production of organic acids, hydrogen peroxide, acetaldehyde, diacetyl, carbon dioxide, or bacteriocins. Lactobacilli are the major contributors to acid production and, hence, a determining factor in the flavor development in kefir. Lactic acid, proteolytic activity, and acetaldehyde are the essential flavor compounds in kefir. Both acid and bacteriocins contribute to the antimicrobial activity of kefir and kefir grains. Kefir is rich in proteins, calcium, vitamin $B_{12}$, niacin, and folic acid. Many studies have investigated the benefits of consuming kefir and have shown that it is a natural probiotic, which when consumed regularly, can help relieve intestinal disorders, promote bowel movement, reduce flatulence, and improve the overall health of the digestive system. Tibetan kefir, which is different from traditional kefir, is produced in China. It has been reported to exhibit antimicrobial activity that is nearly identical to that of traditional kefir. Kefir production is considered a rapidly growing food industry in China.

• Journal of Dairy Science and Biotechnology
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• 제35권1호
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• pp.9-15
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• 2017

Cichorium intybus L. (chicory) roots and leaves are widely used in herbal preparations, which have beneficial effects on the stimulation of digestion and metabolism of food ingredients, gastric juice excretion, diuretic action, and bile excretion. Notably, chicory root is well known as a source of polyphenols, compounds with recognized value in health improvement. In this study, we examined the physicochemical characteristics (TA, pH, and sensory evaluation) of bioactive yoghurt containing different concentrations of chicory. With increasing incubation time (5 h), the TA of the yoghurt increased whereas the pH decreased, regardless of the amount of chicory. As the amount of chicory increased, the scores for color, flavor, taste, and overall acceptability generally decreased. Among the tested groups, yoghurt with the addition of 1% chicory attained the highest scores. Further studies on the production of bioactive yogurt with optimum chicory concentration are needed.

• Journal of Dairy Science and Biotechnology
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• 제34권2호
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• pp.69-74
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• 2016

The main constituent of tea catechins, EGCG [(-)-Epigallocatechin-3-gallate], could inhibit the growth of various microorganisms and differently affect gram-positive and gram-negative bacteria. Antimicrobial activity of EGCG, a compound from green tea (Camellia sinensis) extract, against Cronobacter spp. and Salmonella spp. was studied to evaluate the possibility of using EGCG as a natural food additive in various dairy products. In pure TSB culture, the growth of Cronobacter spp. was suppressed below the detection limit (1 log CFU/mL) depending on EGCG concentration ($600{\sim}800{\mu}g/mL$), after 5~16 days at $4^{\circ}C$. Similarly, the growth of Salmonella spp. was suppressed below the detection limit (1 log CFU/mL) depending on EGCG concentration ($400{\sim}800{\mu}g/mL$), after 5~16 days at $4^{\circ}C$. Therefore, these results suggest that EGCG could be used as an effective additive to inhibit the growth of Cronobacter spp. and Salmonella spp. in various dairy products, such as yoghurt, cheese, dried infant powder, and so on.

• 한국습지학회지
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• 제18권1호
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• pp.32-38
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• 2016

인천광역시 관내에 분포하는 하천 중 시민의 이용 빈도가 높은 장수천을 대상으로 자연형 하천 조성에 따른 영향 및 개선방안을 도출하기 위해 연구를 수행하였으며, 이를 위해 2008년 12월에 준공된 장수천에 대하여 조사 및 분석을 실시하였다. 장수천 수계에 대해 3개 지점을 대상으로 총 3차년도(2009, 2010, 2014)에 걸쳐 조사한 결과 총 3문 5강 11목 26과 56종 980개체의 저서성대형무척추동물이 분포하는 것으로 조사되었다. 이를 각 연차별로 분석해 보면 자연형 하천 공사 직후인 2009년에 18과 30종, 공사 후 2년차인 2010년에는 22과 37종으로 7종이 증가한 것으로 조사되었으며, 이후 2014년 조사 결과 22과 38종으로 2010년 회복 후 안정화 단계에 들어선 것으로 볼 수 있다. 이는 장수천 자연형 하천 조성사업으로 인해 하천 수환경의 개선이 이루어진 것을 확인 할 수 있었으며, 아울러, 장수천의 경우 상류부 지역의 인천대공원 내 양호한 습지지역의 지속적인 유지 관리 노력으로 인해 양호한 생태환경을 보여주고 있으며, 장수천 자연형 하천조성 사업과 함께 긍정적 효과를 내고 있는 것으로 확인되었다.

• 한국환경생태학회지
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• 제36권3호
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• pp.318-326
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• 2022

생물다양성협약(CBD)에서는 전 지구적으로 발생하는 환경문제의 대응방안으로 육상의 17%를 보호지역으로 지정하도록 할 것을 권고하고 있다. 우리나라도 국제적 수준에 맞춰 보호지역을 지정하고 보호지역 지정이 가지는 의미를 설명할 필요성이 생겼으며, 이에 따라 생태계서비스에 관한 연구의 필요성이 요구되고 있다. 우리나라는 보호지역 지정을 자연공원법에 의해 위계별로 국립공원, 도립공원, 군립공원으로 지정하고 있다. 그러나 정치, 행정적 측면이 우선시되면서 생태계서비스 가치평가 연구 및 서식지 관리가 국립공원에 편중되어 도립 및 군립공원은 다소 미비하여 연구의 필요성이 있다. 본 연구에서는 국립공원에 비해 서식지 관리 및 생태계서비스 가치평가 연구가 미비한 가지산도립공원을 대상으로 InVEST 모델 중 InVEST Habitat Quality 모델을 사용하여 서식지 질을 평가하고, 분석 결과를 16개의 산악형 국립공원과 비교하였다. 분석 결과 가지산도립공원의 서식지질 값은 0.83이었으며, 주변 지역에 비해 서식질 질이 높게 나타났다. 3개 지구별 서식지질 차이를 분석한 결과 통도사지구와 내원사지구가 0.84, 석남사지구가 0.83으로 나타났고 용도지구별로는 자연보존지구, 자연환경지구, 문화유산지구, 공원마을지구 순으로 서식지질 값이 높게 나타났다. 기존 국립공원 서식지질 분석 결과와 비교한 결과 가지산도립공원은 무등산국립공원 수준의 자연성을 나타내었다. 이러한 결과는 추후 도립공원의 생물다양성 보전 및 생태계서비스 증진을 위한 정책 수립과 관리방안 수립의 객관적인 자료로 활용될 수 있을 것이다.

• Journal of Ecology and Environment
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• 제37권4호
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• pp.225-229
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• 2014

Feeding behavior of Temora turbinata was investigated through laboratory experiments with special emphasis on its food preference and consequent clearance rate on diatom and microbial components given as common natural food assemblage of coastal area (Uchiumi, Uwa Sea, Japan). Among available prey items, T. turbinata showed the highest clearance rate for Thalassiosira spp. ($0.23{\pm}0.08L\;Temora^{-1}day^{-1}$) followed by Chaetoceros spp. ($0.11{\pm}0.03L\;Temora^{-1}day^{-1}$), but clearance rates for other diatom, Nitzschia spp. was lower (0.03 to $0.07L\;Temora^{-1}day^{-1}$). Bacterial abundances showed no response against 24-h feeding of T. turbinata. Feeding of T. turbinata on heterotrophic nanoflagellates (HNF) was apparent when clearance rates of T. turbinata on diatoms were relatively low, but T. turbinata did not consume HNF as well as ciliates with Thalassiosira spp. of which clearance rate was highest. The results suggest that HNF and ciliates are possible supplementary prey item for T. turbinata, but their contribution as food sources can be limited by the presence of other prey items such as preferable diatom species.