• 한국임상수의학회지
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• 제33권5호
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• pp.266-269
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• 2016

Cell-free fetal RNA is useful to determine fetal sex and detect other inherent genetic disorders. However, non-invasive fetal sex determination methods using fetal RNA from maternal plasma is not yet well established in studies pertaining to bovine animals. Thus, the aim of this study was to systematically evaluate the presence of the male-specific ZRSR2Y gene transcript in maternal plasma using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assays, and to verify its accuracy, sensitivity, and specificity in determining fetal sex between 30 and 100 days of gestation. Overall accuracy, sensitivity, and specificity of the ZRSR2Y gene transcripts in determining fetal sex were 89.1%, 86.3%, and 100%, respectively. The 30 to 100 days of gestation were further classified into five stages of gestation, and each stage had relatively high accurate, sensitive, and specific results. Overall, these results indicate that the expression of the ZRSR2Y gene can be used for fetal sex determination in bovine animals using circulating cell-free RNA in maternal plasma during early pregnancy.

• 농업과학연구
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• 제46권1호
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• pp.195-204
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• 2019

In this study, we examined the effect of a liposome-based extender (Optixcell) and a tris-citric egg-yolk extender (Triladyl) on the frozen-thawed spermatozoa characteristics and the calving rate. The percentages for the total motility of the frozen-thawed spermatozoa were similar in the Optixcell and Triladyl groups. However, among the motile spermatozoa with a straight line velocity (VSL) ${\geq}25{\mu}m/sec$, the curvilinear velocity (VCL, ${\mu}m/sec$), VSL (${\mu}m/sec$), average path velocity (VAP, ${\mu}m/sec$), amplitude of lateral head displacement (ALH, ${\mu}m$), beat cross frequency (BCF, Hz), and plasma membrane integrity of the frozen-thawed spermatozoa for the Optixcell group were significantly higher than those for the Triladyl group. Furthermore, the calving rate in the Optixcell group (79.0%) was higher than that of the Triladyl group (62.8%). However, the acrosomal membrane integrity of the frozen-thawed spermatozoa in the Optixcell and Triladyl groups was not significantly different. These results indicate that semen freezing with Optixcell improved the motility and plasma membrane integrity of frozen-thawed spermatozoa and the calving rate of Hanwoo cows (native Korean cattle). In conclusion, our results suggest that semen freezing with the liposome-based extender Optixcell is more efficient than with the tris-citric egg-yolk extender Triladyl for improved offspring production.

• Journal of Animal Science and Technology
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• 제45권1호
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• pp.57-68
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• 2003

본 시험은 한우 암소에 대한 죽초액의 급여 효과를 구명하기 위하여 비육중인 암소 15두를 대상으로 대조구와 죽초액 3% 및 6% 급여구의 3개 처리구에 5두씩 공시하여 4개월간 사양시험을 실시한 결과는 요약하면 다음과 같다. 일당증체량은 죽초액 3% 급여구에서 약간 증가한 반면에 죽초액 6% 급여구에서는 감소하였다. 사료섭취량은 죽초액 6% 급여구에서 유의적(P<0.05)으로 감소하였다. 혈액성상에서 glucose 농도는 죽초액 3% 및 6% 급여구에서 유의적(P<0.05)으로 감소하였다. Total protein과 cholesterol 농도는 죽초액 3% 및 6% 급여구가 유의적(P<0.05)으로 증가하였다. BUN의 농도는 죽초액 3% 급여구에서 유의적(P<0.05)으로 증가하였다. 도체성적에서 등심단면적은 유의적인 차이가 없었으나 등지방 두께가 죽초액 6%급여구에서 유의적(P<0.05)으로 감소하였다. 근내지방도는 죽초액 3% 급여구에서 유의적(P<0.05)으로 높게 나타나 육질등급이 향상되었다. 죽초액 급여에 의한 부분육 성적에서 별다른 변화가 없었다. 등심의 이화학적 특성에서 조지방 함량이 죽초액 3% 급여구에서 유의적(P<0.05)으로 높게 나타났다. 전단력가와 콜레스테롤 함량은 죽초액 3% 및 6% 급여구에서 유의적(P<0.05)으로 감소하였다. 등심의 지방산 조성에서 16:0은 죽초액 3% 급여구에서 유의적(P<0.05)으로 감소한 반면에 18:1은 죽초액 3% 및 6% 급여구에서 유의적(P<0.05)으로 증가하였다. 따라서 죽초액 3% 급여구에서 포화지방산이 유의적(P<0.05)으로 감소한 반면에 불포화지방산이 유의적(P<0.05)으로 증가하였다. 관능평가에서 죽초액 급여에 의한 냄새와 외관의 개선효과는 없었다. 맛은 죽초액 3%와 6% 급여구가 대조구에 비해 유의적(P<0.05)으로 개선되는 효과를 나타냈다. 이상의 결과를 종합해 보면 한우 암소에 대한 죽초액 3% 급여는 근내지방도 및 조지방 함량의 증가, 전단력가 및 콜레스테롤 함량의 감소, 불포화지방산 비율의 증가, 그리고 관능평가에서 맛에 개선효과를 나타냈다.

• 한국수정란이식학회지
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• 제29권3호
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• pp.265-271
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• 2014

본 연구에서 한우를 공란우로 사용하여 OPU 방법으로 가장 더운 계절의 hot season과 선선한 cool season의 두 계절의 차이에 따른 생성된 난포의 수, 난자 회수율, 난자 등급율, 수정율 및 배반포 발달 능력을 분석하여, 두 계절이 공란우의 번식 능력에 미치는 영향에 관하여 조사하였다. 1. 계절의 영향이 OPU 공란우의 난포 생성 수에 미치는 결과는 난포 생성 개수는 1154개($18.32{\pm}2.26$), 971개($15.41{\pm}3.34$)로 hot season 그룹이 유의적으로 높은 것을 알 수 있었다(p<0.05). 2. 계절에 따른 난자 수 및 난자 회수율은 hot season 그룹의 475개($7.54{\pm}3.14$), 41.16%로 cool season 그룹 448개($7.11{\pm}3.42$), 46.14%와 비교하여 유의적인 차이가 없었다(p<0.05). 3. OPU를 통하여 회수된 두 계절별 난자 등급은 Grade A는 Hot season 그룹 110개($1.75{\pm}1.86$), Cool season 그룹 63개($1.00{\pm}1.46$)로 hot season 그룹이 cool season 그룹과 비교하여 유의적으로 높았다(p<0.05). 하지만 다른 등급인 Grade B는 87개($1.38{\pm}1.60$) vs. 97개($1.54{\pm}1.39$), Grade C는 166개($2.63{\pm}2.43$) vs. 170개($2.70{\pm}2.04$), Grade D는 112개($1.78{\pm}2.65$) vs. 118개($1.87{\pm}1.86$)로 hot season과 cool season 간의 유의적인 차이를 보이지 않았다(p<0.05). 4. 계절에 따른 체외 수정 후의 수정률은 hot season과 cool season 각각 242(66.67%)와 209(63.3%), 배반포 발달율 214(58.95%) vs. 188(56.97%)로 수정률과 배반포 발달율은 유의적인 차이가 없었다(p<0.05). 본 연구의 결과로 계절에 따른 영향에 의해 공란우의 난포생성수와 A등급의 난자 출현율에서 유의적인 차이를 보였다. 하지만 나머지 등급의 난자 출현율, 수정률 및 배반포 발달율은 차이가 없는 것으로 보아, 계절의 차이로 인한 한우 공란우의 번식 능력에 미치는 영향은 미비하다고 판단된다.

• Journal of Animal Science and Technology
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• 제48권2호
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• pp.219-226
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• 2006

본 시험은 산림 간벌 부산물인 잣나무 생지엽을 반추가축 사료로 이용하기 위하여 제조된 발효 생지엽(pine silage)의 한우 반추위내 in situ 분해율 및 in vivo 소화율, 사양성적 및 도체분석에 있어서 볏짚 대체효과를 규명하기 위하여 수행되었다. In situ 시험은 nylon bag 시료를 처리 당 4반복으로 하여, 반추위내에서 48시간 동안 발효시켰으며, in vivo 소화시험은 볏짚 급여구와 생지엽 silage구로 나누어 incomplete double turn over design에 의해 처리 당 5반복으로 수행되었다. 사양시험에서는 생지엽 silage에 대한 볏짚 대체비율을 4수준(0%, 25%, 50%, 75%)으로 나누어 한우수소 48두를 처리당 12두씩 완전임의로 배치하였으며, 시험종료 후, 도체분석을 하였다. 반추위내 in situ 조단백질 분해율은 생지엽 silage가 볏짚보다 높았으나(P<0.01), NDF 분해율은 오히려 낮았다(P<0.01). In vivo 건물, 조단백질 및 NDF 소화율은 처리간 차이가 없었다. 일당 증체량은 볏짚 급여구와 비해 생지엽 silage 25% 대체구를 제외한 모든 생지엽 silage 대체구에서 유의적(P<0.01)으로 높았다. 생지엽 silage 급여 및 급여수준은 사료 효율에 영향을 주지 않았다.도체성적에 있어서 통계적 유의차는 없었으나 근내지방도는 50% 생지엽 silage 대체구가 타 처리구보다 수치적으로 높고, 등지방 두께는 생지엽 silage 대체구가 볏짚 급여구보다 다소 두꺼운 경향을 보였다. 이상의 결과로부터 생지엽 silage를 한우의 조사료로서 사용할 경우, 볏짚과 50%씩 혼합해서 급여하는 것이 건물섭취량과 일당증체량에서 좋은 효과를 기대할 수 있는 것으로 조사되었다.

• 대한수의학회지
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• 제20권2호
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• pp.179-197
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• 1980

A survey was made from March 1978 through Feb. 1979 to know infection rate of parasites and incidence of diseases in 1968 cattle of 240 rearing and fattening cattle herds (1746 Korean native cattle, 186 Holstein, 34 Charolais and 2 Hereford) raising in 28 cities and counties of Gyeongbug district. The results obtained are summarized as follows: 1. The rate of nematoda infection was about 49.0% in the examined cattle. These nematodes were identified as Mecistocirrus sp., Oesophagostomum sp., Bunostomum sp., Trichostrongylus spp., Strongyloides sp. and other 3 species. Infection rate of nematoda in fattening and breeding cattle was higher than that in rearing calves, 44.3% of the positive cattle were infected with 2 to 5 species, and incidence of mixed infection was high in fattening cattle. 2. The rate of coccidal infection was 10.9%. Eimeria $z{\ddot{u}}rni$, Eimeria bovis and Eimeria bukidnonensis were mainly found in the examined cattle. The infection rates of cocidia in Holstein, breeding Korean cows, rearing Korean calves and fattening Korean cattle were 27.3%, 15.8%, 11.2% and 9.1%, respectively. 3. The rate of trematoda was 14.6% with Fasciola spp. (11.2%), Eurytrema spp. (2.0%), and Paramphistomum spp. infections (1.4%). Breeding Korean cows, fattening Korean cattle, Holstein and rearing Korean calves indicated 47.4%, 26.4%, 18.2% and 6.3% incidence of trematoda infections, respectively. of trematoda positive cattle, 76.6% were infected with Fasciola spp., 4. The rate of Theileria and Babesia infection was 62.7% in the examined cattle. Of these positive cattle 5.4% were infected with both parasites, 57.3% with Theileria, and 1.3% of rearing Korean calves and 22.6% of Holstein were found to have parasitized erythrocytes over 1.0%. 5. The incidence of diseases in 1968 cattle of 240 herds was 564 cattle (28.7%) of 154 herds (64.2%). of the diseases observed of skin occurred in 354 cattle (18.0%) of 61 herds (25.4%), diseases of respiratory system in 121 cattle (6.1%) of 34 herds (14.2%), diseases of digestive system in 38 cattle (1.9%) of 22 herds (9.2%), diseases of eye in 21 cattle (1.1%) of 13 herds (5.4%), diseases of urogenital system in 8 cattle (0.4%) of 8 herds (3.3%) and the other diseases in 24 cattle (1.2%) of 22 herds (9.2%). 6. Diseases of skin in 1968 cattle of 240 herds werds were observed in the highest incidence. Ringworm was observed in 13.3% of the examined herds, rearing Korean calves and Holstein showed high incidence. Scabies caused by Chorioptes bovis and alopecia localis along with infestation of lice (Damalinia bovis, Linognathus vituli) were observed in fattening Korean cattlein winter. 7. Diseases of respiratory system occurred frequently in rearing Korean cattle and Holstein. In the diseases of digestive system acute indigestion occurred in fattening Korean cittle, enteritis in rearing Korean calves and Holstein, and coccidiosis in rearing Korean calves maninly. 8. Infectious keratoconjunctivitis occurred in a fattening Korean cattle herd, sterility (2.0%) in breeding Korean cows, and theileriosis in Holstein calves were also occurred. In addition, poisoning and heat stroke were observed in several cattle.

• 한국가축번식학회지
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• 제15권2호
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• pp.125-132
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• 1991

The studies on the carried out to investigate the effects of capacitation method of spermatozoa on the in vitro fertilization and cleavage rate of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible of diameter 3~5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and FCS for 24~48hrs in an incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 12~18hrs with motile capacitated sperm by preincubation of mKRB, treatment of HIS(high strength ion), Ca-IA(Inophore A), BFF(bovine follicular fluids) and heparin. The results obtained in these experiments were summarized as follows : 1. The in vitro fertilizatin and cleavage rate offollicular oocytes fertilized with capacitated spermatozoas in BO solution by preincubation of mKRB, treatment of HIS, Ca-IA, BFF and heparin method were 53.1%, 33.9%, 50.8%, 48.1%, 58.8% and 28.1%, 17.7%, 26.2%, 22.8%, 32.8%, respectively. And the fertilization and cleavage rate of heparin method was of highest of all. 2. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solutin by both caffeine, BSA and heparin methods were 65.8%, 70.3% and 40.8%, 47.3%, respectively, and those rates were higher treatment of heparin＋BSA, heparin＋caffeine than treatment of heparin. 3. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoa in BO solution with heparin concentrations of 2, 5, 10, 20, 40$\mu\textrm{g}$/ml were 50.0%, 54.7%, 58.1%, 51.7% and 27.9%, 32.8%, 37.1%, 30.0%, respectively. And the fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution with 10$\mu\textrm{g}$/ml of heparin was the highest of all. 4. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution containing heparin with caffeine concentraton of 10, 20, 30, 40$\mu\textrm{g}$/ml were 71.4%, 74.3% and 70.6%, 70.0% and 45.7%, 47.3%, 44.1%, 41.4%, respectively. The fertilization and cleavage rate of spermatozoa fertilized in BO solution with caffeine and heparin together(70.3~74.3%) was higher than that of spermatozoa fertilized in BO solution with heparin(58.8%). 5. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution containing heparin with BSA concentration of 5, 10, 20, 30$\mu\textrm{g}$/ml were 63.6%, 62.9%, 66.7%, 60.3% and 44.1%, 43.5%, 48.5%, 42.7%, respectively. The fertilization and cleavage rate of spermatozoa fertilized in BO solution with BSA and heparin together(60.3~66.73%) was higher than that of spermatozoa fertilized in BO solution with heparin(58.8%).

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.263-270
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• 2010

Luteal cells produce progesterone that supports pregnancy. Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism. In the present study, the corpus luteum (CL) in early pregnancy established from luteal phase and pregnant phase was analyzed. The first study determined progesterone changes in the bovine CL at day 19 (early maternal recognition period) and day 90 in mid-pregnancy and compared them to the CL from day 12 of the estrous cycle. CL alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Comparing CL from luteal phase to those from pregnant phase counterparts, significant changes in expression level were found in 23 proteins. Of these proteins 17 were not expressed in pregnant phase CL but expressed in luteal phase counterpart, whereas, the expression of the other 6 proteins was limited only in pregnant phase CL. Among these proteins, vimentin is considered to be involved in regulation of post-implantation development. In particular, vimentin may be used as marker for CL development during pregnancy because the expression level changed considerably in pregnant phase CL tissue compared with its luteal phase counterpart. Data from 2-DE suggest that protein expression was disorientated in mid pregnancy from luteal phase, but these changes was regulated with progression of pregnancy. These findings demonstrate CL development during mid-pregnancy from luteal phase and suggest that alternations of specific CL protein expression may be involved in maintenance of pregnancy.

• 한국수정란이식학회지
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• 제17권1호
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• pp.55-59
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• 2002

ICSI시 동결 융해한 부고환 정자의 이용 가능성을 알아보고자 난자의 배양시 체외성숙율과 활성화 처리를 한 난자와 동결 융해한 부고환 정자로 ICSI시 체외발생율을 조사하였으며, 결과를 요약하면 다음과 같다. 1. 난포란을 회수 후 24시간 배양하였을 때 배양 시간에 따른 GV, MI, M II로의 체외성숙율은 각각 7/60(11.7%), 5/60(8.3%), 48/60(80.0%)였고 30시간 배양 시간에 따른 GV, MI, M II로의 체외성숙율은 각각 3/60(5.0%), 4/60(6.7%), 53/60(88.3%)였고 퇴화란은 각각 2/60(3.3%)와 1/60(1.7%)였다. 2. 동결 융해한 부고환 정자를 이용하여 활성화 처리를 한 난자에 ICSI를 하였을 때 상실배와 배반포로의 체외발생율은 각각 12/46(26.1%), 22/46 (47.8%)로서 비활성화처리 난자군 5/39 (12.8%), 10/39(25.6%)에 비해 높은 체외발생율을 나타냈다. 3. 활성화 처리를 한 난자에 신선정자, 부고환 정자 및 동결 융해한 부고환 정자로 ICSI시 체외 발생율은 각각 24/45(53.3%), 15/40(37.50%), l1/43 (25.6%)로서 신선정자에 비해 동결 융해한 부고환 정자처리군은 체외발생율은 약간 낮았지만 이용 가능성이 있음을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권7호
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• pp.1202-1208
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• 2020

Objective: The aim of this study was to identify a distribution pattern of meat quality grade (MQG) as a function of carcass yield index (CYI) and the gender of Hanwoo (bull, cow, and steer) to determine the optimum point between both yield and quality. We also attempted to identify how pre- and post-deboning variables affect the gender-specific beef quality of Hanwoo. Methods: A total of 31 deboning variables, consisting of 7 pre-deboning and 24 post-deboning variables from bulls (n = 139), cows (n = 69), and steers (n = 153), were obtained from the National Institute of Animal Science (NIAS) in South Korea. The database was reconstructed to be suitable for a statistical significance test between the CYI and the MQG as well as classification of meat quality. Discriminant function analysis was used for classifying MQG using the deboning parameters of Hanwoo by gender. Results: The means of CYI according to 1+, 1, 2, and 3 of MQG were 68.64±2.02, 68.85±1.94, 68.62±5.88, and 70.99±3.32, respectively. High carcass yield correlated with low-quality grade, while high-quality meat most frequently was obtained from steers. The classification ability of pre-deboning parameters was higher than that of post-deboning parameters. Moisture and the shear force were the common significant parameters in all discriminant functions having a classification accuracy of 80.6%, 71%, and 56.9% for the bull, cow, and steer, respectively. Conclusion: This study provides basic information for predicting the meat quality by gender using pre-deboning variables consistent with the actual grading index.