• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.158-158
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.209-209
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• 2004

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2004.05a
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• pp.296-298
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• 2004

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.103-116
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• 2020

Freshwater ecosystems provide a complex environment for microorganisms. In this study, we isolated diverse fungal strains from plant litter in freshwaters. These strains were identified using molecular phylogenetic analyses of rDNA and/or other gene sequences (TUB, GAPDH, and EF1). In addition, we examined their morphological characteristics by microscopy and cultural characteristics on several media. We identified six previously unrecorded Sordariomycetes species in Korea, i.e., Colletotrichum godetiae, Discosia rubi, Robillarda sessilis, Monochaetia dimorphospora, Idriella lunata, and Phialemoniopsis endophytica. Of these, D. rubi and M. dimorphospora exhibited high extracellular amylase, lipase, and protease activities, suggesting that these fungal isolates might play an important role as decomposers in freshwater ecosystems. Plant litter could thus be a good source for isolating and investigating previously undocumented fungal species in freshwater environments.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.523-531
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• 2020

This study focused on the isolation and characterization of wild yeasts in Korea. The yeasts were identified by phylogenetically analyzing the D1/D2 domains of the 26S rDNA regions. Consequently, we identified seven strains, NNIBRFG856, NNIBRFG3732, NNIBRFG3734, NNIBRFG3738, NNIBRFG3739, NNIBRFG5497, and NNIBRFG6049, which were confirmed to be Kabatiella microsticta, Pichia membranifaciens, Candida vartiovaarae, Candida sake, Debaryomyces hansenii, Candida railenensis, and Schwanniomyces polymorphus, respectively, all of them being new in Korea. Morphological and cultural characteristics of these yeast species were investigated. None of the strains formed ascospores or pseudomycelia. Moreover, these yeasts grew in a pH range of 4-8. NNIBRFG3732, NNIBRFG3738, NNIBRFG3739, NNIBRFG5497, and NNIBRFG6049 were halotolerant or halophilic, and NNIBRFG3732, NNIBRFG3734, and NNIBRFG6049 grew in vitamin-free medium. NNIBRFG3732, NIBRFG3739, and NNIBRFG6049 grew at 35 ℃, but not at 40 ℃.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.423-443
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• 2020

Freshwater fungi are a poly-phylogenetic group of taxonomically diverse organisms. In this study, we isolated diverse fungal strains from various environmental samples obtained from freshwaters in Korea. These strains were identified by performing molecular phylogenetic analyses of rDNA and/or other sequences (beta-tubulin, RNA polymerase II, and translation elongation factor 1). In addition, we examined their morphological characteristics microscopically and cultural characteristics using different media. We identified eleven unrecorded Pezizomycotina species: Cladosporium angulosum, Pseudorobillarda phragmitis, Paraconiothyrium estuarinum, Pseudopithomyces palmicola, Pyrenochaetopsis paucisetosa, Thelebolus globosus, Plagiostoma mejianum, Trichoderma cremeum, Fusarium tanahbumbuense, Coniochaeta endophytica, and Chaetomium tenue. Environmental samples obtained from different freshwater ecosystems in Korea could thus be a good source for isolating and investigating novel fungal species.

• Molecules and Cells
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• v.26 no.6
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• pp.595-605
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• 2008

Genome wide transcription analysis in response to stresses is essential to provide the basis of effective engineering strategies to improve stress tolerance in crop plants. In order to perform transcriptome analysis in Brassica rapa, we constructed a B. rapa oligo microarray, KBGP-24K, using sequence information from approximately 24,000 unigenes and analyzed cold ($4^{\circ}C$), salt (250 mM NaCl), and drought (air-dry) treated B. rapa plants. Among the B. rapa unigenes represented on the microarray, 417 (1.7%), 202 (0.8%), and 738 (3.1%) were identified as responsive genes that were differently expressed 5-fold or more at least once during a 48-h treatment with cold, salt, and drought, respectively. These results were confirmed by RT-PCR analysis. In the abiotic stress responsive genes identified, we found 56 transcription factor genes and 60 commonly responsive genes. It suggests that various transcriptional regulatory mechanisms and common signaling pathway are working together under the abiotic stresses in B. rapa. In conclusion, our new developed 24K oligo microarray will be a useful tool for transcriptome profiling and this work will provide valuable insight in the response to abiotic stress in B. rapa.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.447-454
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• 2021

This study was conducted to evaluate the effects of different copper sources (inorganic and organic) on the growth performance, fecal copper excretion, intestinal morphology, and health in growing pigs. A total of 40 growing pigs (30.22 ± 1.92 kg) were randomly assigned to 5 dietary treatments: a basal control diet (CON), 4 experimental diets supplemented with either copper sulfate (CuSO₄), Cu-glycine complex (CuGly), Cu-amino acid complex (CuAA), or Cu-hydroxy-4-methylthio butanoate chelate complex (CuHMB) at 100 ppm, respectively. At the end of the study (28 days), fecal and blood samples were collected, and the pigs were slaughtered to determine the intestinal morphology. During the 28 days of the experimental period, pigs fed the inorganic and organic copper showed a higher average daily gain (p < 0.01) and gain feed ratio (p < 0.01). There were no differences in mineral concentrations of the serum; however, the copper concentration of the feces was lower (p < 0.01) in the CuAA and CuHMB groups. The intestinal morphology and blood profiles did not significantly differ between the groups. In conclusion, the organic copper sources (CuAA and CuHMB) can be used as a growth promoter to replace the CuSO₄ without any negative effects on health in growing pigs and to reduce fecal copper excretion.

• Quality Improvement in Health Care
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• v.6 no.1_2
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• pp.92-106
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• 1999

Background : In many university hospitals, intravenous(IV) therapies and samplings had been one of the most important works of doctors who are in training. However, recently as patient oriented care is becoming more weighted for qualified health service IV therapies should be the works of specialized personnels. This study was conducted to investigate the medical staff's perception on IV team, to survey patient or parent's expectation on IV team, and to assess the frequency of IV therapy related complications and the characteristics of phlebitis among the hospitalized children. Methods : We collected data prospectively before the start of IV team from February 22 to February 29. 1999 and from September 27 to October 3, 1999, 6 months after beginning of IV team at Seoul National University Children's Hospital. IV team started their activities from March 1, 1999. General pediatric wards were not included for the IV and sampling team and oncology ward and surgical units were all included for the IV and sampling team's work. IV specialist was the well trained nurse who had been working in the field of pediatrics especially for the oncology patients. The subjects of this study were medical staffs who were working in children's hospital as doctors in training and patients who were treated with IV therapies in children's hospital during the same period. Results : Doctors responded that IV team need to be organized for IV care and expected IV team could reduce their work load. Parents of patients also responded IV team was very improtant to perform high quality IV care. They had willingness to pay extra charge for IV team care. In the wards where IV team did not work, they used various kinds and sizes of catheters, but in the wards where IV team worked, they needed just one or two types of catheters. As the exact role of IV team is not still established, job description is needed between the IV team and medical doctors. In the aspects of medical costs. it could save the materials for the IV also. Conclusion : This study showed that IV team could increase patient's satisfaction with decrease of medical doctors work load and concomitantly could save the costs of IV materials. And for the expansion of the IV team, job description is needed and for the total care of the children IV specialist and sampling team should expand their roles.

• International journal of advanced smart convergence
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• v.13 no.1
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• pp.152-161
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• 2024

The purpose of this study sought to determine the impact of shared leadership perceived by organizational members on team effectiveness and team learning behavior. For this purpose, the results of the empirical analysis of 206 organizational members are as follows. First, shared leadership was analyzed to improve team effectiveness. Second, shared leadership had a positive effect on team learning behavior. Third, team learning behavior was statistically significantly analyzed for team effectiveness. This study confirmed the importance of shared leadership, which has a positive impact on team effectiveness and team learning behavior. This may require building a new culture that can demonstrate the inherent leadership of organizational members in the influence relationship between shared leadership, team effectiveness, and team learning behavior. In other words, in order to systematically demonstrate and implement shared leadership, the execution ability of executives, managers, and working-level managers is important. To this end, it is necessary to build an organizational culture that matches the characteristics of the organization and develop and continuously implement human resource development systems and programs that can implement this.