• Journal of Applied Biological Chemistry
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• 제51권4호
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• pp.172-175
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• 2008

• 한국미생물·생명공학회지
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• 제51권3호
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• pp.328-331
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• 2023

Genomic information of biotechnologically and industrially important microorganisms provides the basis for understanding their metabolic potential. Here, we report the draft genome sequence of the Neodothiora populina-like yeast strain JAF-11 capable of producing biosurfactant myo-inositol lipids. The draft genome contained genes associated with secondary metabolite biosynthesis, including transport and metabolism of lipids, which are a major component of fungal surfactants. Identification of myo-inositol and acyl chain synthesis genes in the draft genome corresponded to the specific biosurfactant produced by strain JAF-11. Further experimental studies could help to elucidate the genes responsible for the production of biosurfactant by strain JAF-11.

• The Plant Pathology Journal
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• 제33권5호
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• pp.499-507
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• 2017

In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani. The butanol extract of the culture filtrate of B. amyloliquefaciens DA12 highly inhibited the germination of F. graminearum macroconidia with inhibition rate 83% at a concentration of $31.3{\mu}g/ml$ and 100% at a concentration of $250{\mu}g/ml$. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of Bacillus amyloliquefaciens DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of Fusarium diseases and mycotoxin contamination of crops.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.49.1-49.1
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.121-121
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• 2022

• 한국어병학회지
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• 제21권3호
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• pp.259-270
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• 2008

Disease surveillance was performed to monitor the prevalence of fish pathogens in wild marine fish caught in coastal offshore water in Korea. A total of 333 of fish samples were collected at set net or fish market at landing port in Pohang (East Sea), Taean (Western Sea), Goseong and Tongyeong (Southern Sea) and 21 species of pathogens causing clinical infections to farmed fish were investigated. The detection rates of fish pathogens from Mugili formes, Tetraodontiformes, Pleuroneciformes, Sorpaeniformes, erciformes and Clupeiformes were 90.9, 61.1, 47.6, 43.6, 37.2 and 11.8%, respectively. Comparing with prevalence of diseases seasonally, both the detection rates of bacteria and parasite were higher than those of virus in April but the detection rates of parasites were distinctively higher than those of bacteria in August with high water temperature. Virus were detected in fish samples caught in the Western and Southern Sea in April. The detected parasites were Trichodina, Ichthyophthirius, Dactylogyrus, Microcotyle, Bivagina, Caligus, Alella and Myxobolus. Among the bacterial pathogens, Vibrio, Streptococcus, Photobacterium, Psuedomonas were predominant. Viral nervous necrosis virus (VNNV) and flounder lymphocystis disease virus (FLDV) were detected from the 6 species of fish virus examined in this study.

• Reproductive and Developmental Biology
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• 제37권4호
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• pp.219-224
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• 2013

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson's, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.

• 한국식품영양학회지
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• 제32권3호
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• pp.226-235
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• 2019

In this study, the product characteristics and physicochemical properties were investigated through collection of commercial porridge. The addition rate of grain raw materials was about 6.5~11.75%, glutinous rice was added at a rate of about 23~60% to improve the viscosity and various other food additives were used. The moisture content characteristics varied among the products. The rotational viscosity of CP (Commercial Porridge)4 was the highest at 39,054 cP, while the flow viscosity of CP3 was least at 4.80 cm/30 seconds. The starch content differed among the products in the range of total starch 6.96~8.08%, amylose 1.41~2.61%, total sugar 6.55~12.81% and reducing sugar 0.50~0.99%. Particularly, total sugar showed a very high correlation (-0.920) while rotational viscosity and color value (b) showed significant correlation with most of the properties i.e. moisture, solids content etc. There was a rapid increase in the reactivity of starch degrading enzyme at the early stage of the reaction which gradually decreased with time. The physicochemical characteristics of commercial porridge presented in this study could be expected to increase the industrial use value of the related research because it considers the quality of the currently commercialized porridge for the future selection of suitable porridge raw materials.

• 한국동물위생학회지
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• 제44권2호
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• pp.85-91
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• 2021

This study was performed to identify the prevalence of cryptosporidiosis and giardiasis in diarrhea feces of Korean native calves raised in Gimje, Jeollabuk-do, with a rapid diagnostic kit. The prevalence of both diseases were classified according to the age of calf, season of sampling period, and sampled farm where diarrheic calf is commonly observed. In diarrhea feces of 431 calves, cryptosporidiosis had a high incidence rate between 1 and 3 weeks of age, whereas giardiasis showed a high incidence between 4 and 6 weeks of age. It is thought that the incidence of protozoan diseases is markedly influenced by the calf's postnatal age. Regarding with the sampling season, the prevalence of cryptosporidiosis and giardiasis in calves was significantly higher in spring and summer than in autumn and winter (P<0.05). Among the sampled farms where both diseases are commonly observed, cryptosporidiosis was more frequently detected than giardiasis in calves. Particularly, in farms where cryptosporidiosis is common, it tends to be a disease that is difficult to eradicate as it occurs continuously throughout the year. Hygiene management such as regular cleaning and disinfection of the livestock and the surrounding environments is considered necessary to reduce and prevent damage from these protozoal diseases.

• International Journal of Computer Science & Network Security
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• 제22권7호
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• pp.283-293
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• 2022

One of the important areas of state policy in the socio-economic and cultural development of the country is cultural diplomacy. It contributes to the information dissemination about the country, strengthens interstate relations, and forms a positive image. Through cultural diplomacy, we achieve a positive perception of the world community of the country, determined by its place in the modern system of international relations. The aim of the study is a comparative analysis of cultural diplomacy opportunities for sustainable development at different levels of public relations, as well as the impact of cultural diplomacy opportunities on the indicators of the Global Sustainable Competitiveness Index and the Global Sustainable Development Index. Regarding the results of the research on the impact of cultural diplomacy opportunities on the indicators of the Global Index of Sustainable Competitiveness and the Global Index of Sustainable Development, four groups are identified among the countries of the European Union: countries with a very high level of sustainable competitiveness and sustainable development; countries with a high level of sustainable competitiveness and sustainable development; countries with low levels of sustainable competitiveness and sustainable development.