• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권3호
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• pp.210-218
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• 2021

Recently, pest-resistant living modified (LM) crops developed using RNA interference (RNAi) technology have been imported into South Korea. However, the potential adverse effects of unintentionally released RNAi-based LM crops on non-target species have not yet been reported. Coccinella septempunctata, which feeds on aphids, is an important natural enemy insect which can be exposed to the double-stranded RNA (dsRNA) produced by RNAi-based LM plants. To assess the risk of ingestion of Snf7 dsRNA by C. septempunctata, we first identified the species through morphological analysis of collected insects. A method for species identification at the gene level was developed using a specific C. septempunctata 12S rRNA. Furthermore, an experimental model was devised to assess the risk of Snf7 dsRNA ingestion in C. septempunctata. Snf7 dsRNA was mass-purified using an effective dsRNA synthesis method and its presence in C. septempunctata was confirmed after treatment with purified Snf7 dsRNA. Finally, the survival rate, development time, and dry weight of Snf7 dsRNA-treated C. septempunctata were compared with those of GFP and vATPase A dsRNA control treatments, and no risk was found. This study illustrates an effective Snf7 dsRNA synthesis method, as well as a high-concentration domestic insect risk assessment method which uses dsRNA to assess the risk of unintentional released of LM organisms against non-target species.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제55권4호
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• pp.431-436
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• 2022

For the safety assessment of microbiological and chemical hazards in katsuobushi, fifteen samples of katsuobushi were purchased from supermarkets. The contamination levels of total viable bacteria, coliforms, Escherichia coli, and nine pathogenic bacteria [Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens, Enterohemorrhagic E. coli (EHEC), Yersinia enterocolitica and Campylobacter jejuni/coli] were quantitatively or qualitatively assessed. Additionally, the heavy metals (total and methyl mercury) content, radioactivity (¹³¹ I, ¹³⁴ Cs⁺ and ¹³⁷ Cs) were quantitatively assessed. Microbial and chemical analyses were performed using standard methods in Korean food code. The contamination level of total viable bacteria was 2.70 (1.18-4.42) log CFU/g. Coliforms, E. coli and S. aureus were not detected in any samples. Other eight pathogenic bacteria were negative in all samples. The contamination levels of total and methyl mercury were 0.366 (0.227-0.481) and 0.120 (0.002-0.241) mg/kg, respectively. In addition, radioactivity was not detected in any samples. The results will be helpful in revitalizing domestic use and boosting exports of katsuobushi because the microbiological and chemical safety of katsuobushi has been assured. Furthermore, the results may be used as a basis for performing chemical and microbial risk assessments of katsuobushi.

• Journal of Environmental Health Sciences
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• 제44권6호
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• pp.515-523
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• 2018

Objectives: Based on the results of a chemical substance emissions survey, we investigated characteristics of chemical emissions in industrial complexes and used them as basic data for chemical management. Methods: The emissions and characteristics of chemicals by major industrial complexes from 2011 to 2015 were analyzed using the Pollutant Release and Transfer Register homepage. To understand the status of chemical accidents for major chemicals emitted from the industrial complexes, the Chemistry Safety Clearing-house system of the National Institute of Chemical Safety was used. Results: Emissions from the top five industrial complexes accounted for about 30% of total chemical emissions. The chemical emission was the highest in the order of Ulsan Mipo Industrial Complex and Okpo Industrial Complex. The main chemicals emitted were xylene, ethylbenzene, ethyl acetate, toluene, dichloromethane, and others. Carcinogen emissions differed by industrial complex, but ethylbenzene and dichloromethane were the major chemicals for this type of emissions. Conclusion: Recently, the use and emission of chemicals have been continuously increasing. A chemical management plan should be prepared considering the characteristics of industrial complexes and chemical substance emissions.

• Journal of Korean Society of Disaster and Security
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• 제10권2호
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• pp.35-41
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• 2017

Recently chemical intoxication related with the use of chemical extinguishing agents occurs frequently. With the industrialization, high-rising of building and increase of fire risk, we use the various extinguishing agents and the safe use become important. In this study I carried out the risk assessment of representative chemical extinguishing agents (HCFC-123, HFC-125) using the CHARM and got the meaningful qualitative outcome. This study is significant in that the risk assessment of chemicals was conducted using CHARM, chemical risk assessment tool. It is expected that the results will be utilized as the basic data for the national chemical safety management.

• Safe Food
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• 제5권2호
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• pp.14-19
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• 2010

• Proceedings of the Korean Nuclear Society Conference
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• 한국원자력학회 2005년도 추계학술발표회
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• pp.600-601
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• 2005

• Journal of the Korean Society of Safety
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• 제21권3호
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• pp.59-66
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• 2006

Injury Ratio Survey Regulations(IRS) was introduced to the construction industry in the Republic of Korea since 1992 and brought positive effect on occupational accidents reduction. There were tremendous decrease of injury ratios and enforcing of contractors' safety organizations from the beginning of IRS. In spite of these positive results, there were some negative effects such as contractors' shrinking injury reports to keep good injury ratios since these figures had a great impact on pre-qualification stage of bidding when general contractors were competing for new construction projects. Thus, this study aims to devote on lessening construction injury and elimination of above negative impacts through the quantitative statistic analysis of the effectiveness on the occupational accidents prevention of IRS. According to this assessment, there were decrease of from 6.37% to 44.34% in the accident ratios compared to those of non-IRS groups and decrease of from 3.32% to 83.51 % in the accident ratios compared to those of general industry including the unreported accidents.

• Nuclear Engineering and Technology
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• 제42권1호
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• pp.37-46
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• 2010

The development of advanced nuclear fuel cycle(ANFC) technology is essential to meet the national mission for energy independence via a nuclear option in Korea. The action target is to develop environmentally friendly, cost-effective measures to reduce the burden of long term disposal. The proper scenarios regarding potential radionuclide release from a repository have been developed in this study based on the advanced korean Reference Disposal System(A-KRS). To predict safety for the various scenarios, a new assessment code based on the GoldSim software has also been developed. Deterministic analysis indicates an environmental benefit from the ANFC as long as the solid waster from the ANFC act as a proper barrier.

• Journal of the Korea Safety Management & Science
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• 제19권4호
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• pp.53-62
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• 2017

It has come to attention that a risk-assessing organization, that will benchmark a company's safety department, is imperative, following an increase in large-scale SOC-business project, construction of higher-raised buildings, development of underground space; all that have increase accident rates. Having faced problems that arise in firms that demand diversity, complexity and instantaneity, the purpose of the thesis is to arrive at efficient and practical problem-solving means. In order to solve the problems that would surface theoretically during an actual risk assessment, the state of the operation systems of the top five national construction firms having a hazard rate of 0.25 times less than the average rate have been analyzed, while a hierarchal recognition research of the employees who not only function at the operating level but are the practice subjects of a firm, has also been conducted, bringing the main text.

• Journal of the Earthquake Engineering Society of Korea
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• 제25권6호
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• pp.275-281
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• 2021

In this study, algorithms for analyzing the torsion of buildings under earthquake excitation are developed. The algorithm and formulations to account for the torsional angle are verified by analyzing the seismic acceleration time history data. The method was applied to the reference buildings to examine their operation and usability. The reference application demonstrated that the noise-canceling scheme successfully overcame various obstacles in the field measurements. The developed method is expected to be used as a tool to support a loss assessment system for determining the direction and priority of disaster response in the event of an earthquake.