• BioChip Journal
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• v.12 no.4
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• pp.340-347
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• 2018

$F{\ddot{o}}rster$ resonance energy transfer (FRET) is extremely sensitive to the separation distance between the donor and the acceptor which is ideal for probing such biological phenomena. Also, FRET-based probes have been developing for detecting an unamplified, low-abundance of target DNA. Here we describe the development of FRET based DNA sensor based on an accumulated QD system for detecting KRAS G12D mutation which is the most common mutation in cancer. The accumulated QD system consists of the polystyrene beads which surface is modified with carboxyl modified QDs. The QDs are sandwich-hybridized with DNA of a capture probe, a reporter probe with Texas-red, and a target DNA by EDC-NHS coupling. Because the carboxyl modified QDs are located closely to each other in the accumulated QDs, these neighboring QDs are enough to transfer the energy to the acceptor dyes. Therefore the FRET factor in the bead system is enhancing by the additional increase of 29.2% as compared to that in a single QD system. These results suggest that the accumulated nanobead probe with conjugated QDs can be used as ultrasensitive DNA nanosensors detecting the mutation in the various cancers.

• Advances in nano research
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• v.6 no.4
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• pp.399-422
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• 2018

Unwanted vibration is an issue in many industrial systems, especially in nano-devices. There are many ways to compensate these unwanted vibrations based on the results of the past researches. Elastic medium and smart material etc. are effective methods to restrain unnecessary vibration. In this manuscript, dynamic analysis of viscoelastic nanosensor which is made of functionally graded (FGM) nanobeams is investigated. It is assumed that, the shaft is flexible. The system is modeled based on Timoshenko beam theory and also environmental condition, external linear varying loads and thermal loading effect are considered. The equations of motion are extracted by using energy method and Hamilton principle to describe the translational and shear deformation's behavior of the system. Governing equations of motion are extracted by supplementing Eringen's nonlocal theory. Finally vibration behavior of system especially the frequency of system is developed by implementation Semi-analytical differential transformed method (DTM). The results are validated in the researches that have been done in the past and shows good agreement with them.

• Structural Engineering and Mechanics
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• v.69 no.2
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• pp.131-143
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• 2019

In this study, the nonlinear vibration analysis of the composite nanoplate is studied. The composite nanoplate is fabricated by the functional graded (FG) core and lipid face sheets. The material properties in the FG core vary in three directions. The Kelvin-Voigt model is used to study the viscoelastic effect of the lipid layers. By using the Von-Karman assumptions, the nonlinear differential equation of the vibration analysis of the composite nanoplate is obtained. The foundation of the system is modeled by the nonlinear Pasternak foundation. The Bubnov-Galerkin method and the multiple scale method are used to solve the nonlinear differential equation of the composite nanoplate. The free and force vibration analysis of the composite nanoplate are studied. A comparison between the presented results and the reported results is done and good achievement is obtained. The reported results are verified by the results which are obtained by the Runge-Kutta method. The effects of different parameters on the nonlinear vibration frequencies, the primary, the super harmonic and subharmonic resonance cases are investigated. This work will be useful to design the nanosensors with high biocompatibility.

• Advances in nano research
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• v.10 no.5
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• pp.481-491
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• 2021

Graphene Nanosheets play an important role in nanosensors due to their proper surface to volume ratio. Therefore, the main purpose of this paper is to consider the nonlinear vibration behavior of graphene nanosheets (GSs) under the influence of electromagnetic fields and electrical current create forces. Considering more realistic assumptions, new equations have been proposed to study the nonlinear vibration behavior of the GSs carrying electrical current and placed in magnetic field. For this purpose, considering the influences of the magnetic tractions created by electrical and eddy currents, new relationships for electromagnetic interaction forces with these nanosheets have been proposed. Nonlinear coupled equations are discretized by Galerkin method, and then solved via Runge-Kutta method. The effect of different parameters such as size effect, electrical current magnitude and magnetic field intensity on the vibration characteristics of GSs is investigated. The results show that the magnetic field increases the linear natural frequency, and decreases the nonlinear natural frequency of the GSs. Excessive increase of the magnetic field causes instability in the GSs.

• Clinical and Experimental Reproductive Medicine
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• v.47 no.4
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• pp.245-262
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• 2020

In recent years, nanotechnology has revolutionized global healthcare and has been predicted to exert a remarkable effect on clinical medicine. In this context, the clinical use of nanomaterials for cancer diagnosis, fertility preservation, and the management of infertility and other pathologies linked to pubertal development, menopause, sexually transmitted infections, and HIV (human immunodeficiency virus) has substantial promise to fill the existing lacunae in reproductive healthcare. Of late, a number of clinical trials involving the use of nanoparticles for the early detection of reproductive tract infections and cancers, targeted drug delivery, and cellular therapeutics have been conducted. However, most of these trials of nanoengineering are still at a nascent stage, and better synergy between pharmaceutics, chemistry, and cutting-edge molecular sciences is needed for effective translation of these interventions from bench to bedside. To bridge the gap between translational outcome and product development, strategic partnerships with the insight and ability to anticipate challenges, as well as an indepth understanding of the molecular pathways involved, are highly essential. Such amalgamations would overcome the regulatory gauntlet and technical hurdles, thereby facilitating the effective clinical translation of these nano-based tools and technologies. The present review comprehensively focuses on emerging applications of nanotechnology, which holds enormous promise for improved therapeutics and early diagnosis of various human reproductive tract diseases and conditions.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.444-444
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• 2011

Recently, patterned magnetic films and elements attract a wide interest due to their technological potentials in ultrahigh-density magnetic recording and spintronic devices. Among those patterned magnetic structures, magnetic anti-dot patterning induces a strong shape anisotropy in the film, which can control the magnetic properties such as coercivity, permeability, magnetization reversal process, and magneto-resistance. While majority of the previous works have been concentrated on anti-dot arrays with a single magnetic layer, there has been little work on multilayered anti-dot arrays. In this work, we report on study of the magnetic properties of bilayered anti-dot system consisting of upper perforated Co layer of 40 nm and lower continuous Ni layer of 5 nm thick, fabricated by photolithography and wet-etching processes. The magnetic hysteresis (M-H) loops were measured with a superconducting-quantum-interference-device (SQUID) magnetometer (Quantum Design: MPMS). For comparison, investigations on continuous Co thin film and single-layer Co anti-dot arrays were also performed. The magnetic-domain configuration has been measured by using a magnetic force microscope (PSIA: XE-100) equipped with magnetic tips (Nanosensors). An external electromagnet was employed while obtaining the MFM images. The MFM images revealed well-defined periodic domain networks which arise owing to the anisotropies such as magnetic uniaxial anisotropy, configurational anisotropy, etc. The inclusion of holes in a uniform magnetic film and the insertion of a uniform thin Ni layer, drastically affected the coercivity as compared with single Co anti-dot array, without severely affecting the saturation magnetization ($M_s$). The observed changes in the magnetic properties are closely related to the patterning that hinders the domain-wall motion as well as to the magneto-anisotropic bilayer structure.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.44-45
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• 2010

We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.