• 생체재료학회지
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• 제22권4호
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• pp.337-345
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• 2018

Background: Human mesenchymal stem cells (hMSCs) are, due to their pluripotency, useful sources of cells for stem cell therapy and tissue regeneration. The phenotypes of hMSCs are strongly influenced by their microenvironment, in particular the extracellular matrix (ECM), the composition and structure of which are important in regulating stem cell fate. In reciprocal manner, the properties of ECM are remodeled by the hMSCs, but the mechanism involved in ECM remodeling by hMSCs under topographical stimulus is unclear. In this study, we therefore examined the effect of nanotopography on the expression of ECM proteins by hMSCs by analyzing the quantity and structure of the ECM on a nanogrooved surface. Methods: To develop the nanoengineered, hMSC-derived ECM, we fabricated the nanogrooves on a coverglass using a UV-curable polyurethane acrylate (PUA). Then, hMSCs were cultivated on the nanogrooves, and the cells at the full confluency were decellularized. To analyze the effect of nanotopography on the hMSCs, the hMSCs were re-seeded on the nanoengineered, hMSC-derived ECM. Results: hMSCs cultured within the nano-engineered hMSC-derived ECM sheet showed a different pattern of expression of ECM proteins from those cultured on ECM-free, nanogrooved surface. Moreover, hMSCs on the nano-engineered ECM sheet had a shorter vinculin length and were less well-aligned than those on the other surface. In addition, the expression pattern of ECM-related genes by hMSCs on the nanoengineered ECM sheet was altered. Interestingly, the expression of genes for osteogenesis-related ECM proteins was downregulated, while that of genes for chondrogenesis-related ECM proteins was upregulated, on the nanoengineered ECM sheet. Conclusions: The nanoengineered ECM influenced the phenotypic features of hMSCs, and that hMSCs can remodel their ECM microenvironment in the presence of a nanostructured ECM to guide differentiation into a specific lineage.

• Journal of Industrial and Engineering Chemistry
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• 제67권
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• pp.80-91
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• 2018

Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.

• 폴리머
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• 제35권6호
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• pp.499-504
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• 2011

본 연구팀은 탈미네랄화 골분(DBP)이라는 천연재료를 졸(sol)화시켜 poly(lactide-co-glycolide) (PLGA)에 함침시킨 지지체를 개발하였다. DBP를 함침시킨 PLGA 지지체 상의 세포증식과 모폴로지를 평가하기 위해 MTT 분석과 SEM을 측정하였다. 또한 sGAG와 콜라겐 함량 측정과 파종된 연골 세포의 표현형 유지에 미치는 영향을 확인하였다. 그 결과 PLGA에 DBP를 함침시킨 지지체가 PLGA 지지체보다 높은 세포 증식률을 보였다. 또한 파종된 연골세포의 표현형 유지에도 긍정적인 영향을 미치는 것을 확인하였다. 이번 연구 결과를 토대로 PLGA에 DBP를 이용한 용액을 함침시킴으로써 DBP내의 성장인자와의 상호작용을 통해 연골세포의 성장에 긍정적 영향을 미쳐 안정되게 연골을 조직화할 수 있는 연골조직공학 지지체로 적합할 것으로 예상된다.