• Journal of the Korean Chemical Society
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• v.53 no.2
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• pp.111-117
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• 2009

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.05a
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• pp.290-293
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• 2009

This paper presents the development of a disposable label-free biosensor for bio molecular interaction analysis. Label-free biosensors have advantages of high performance in sensitivity and short detection time. Among various label-free systems, we introduced biosensor with nano grating structures based on white light source and spectrometer. And to develop high efficiency label-free biosensor, we suggest replicating processes satisfying required specification. We also report a system set-up to evaluate the characteristics of phenomenon shown in this biosensor system.

• Proceedings of the Materials Research Society of Korea Conference
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• 2007.11a
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• pp.3.1-3.1
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• 2007

• Journal of the Korean Society for Precision Engineering
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• v.25 no.11
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• pp.7-14
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• 2008

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.3
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• pp.267-272
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• 2006

Optical waveguide based on symmetric and asymmetric Mach-Zehnder interferometer(MZI) type was designed, fabricated and measured the optical characteristics for the application of biosensor. The wavelength of the input optical signal for the device was 1550 nm. And the difference of refractive index was $0.45\;{\Delta}\%$ between core and cladding of the device. The TM(Transverse Magnetic) mode optical properties of the biosensor were analyzed with the refractive index variation of gold thin film deposited for overclad. Nowadays, nano-photonic crystal structures have been paied much attention for its high optical sensitivity. There is a technique to realize the structure, which is called Dip-Pen Nanolithography(DPN) process. The process requires a nano-scale process patterning resolution and high reliability. In this paper, two dimensional nano-photonic crystal array on the surface was proposed for improving the sensitivity of optical biosensor. And the Dip-Pen Nanolithogrphy process was investigated to realize it.

• Journal of the Korean Chemical Society
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• v.53 no.3
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• pp.279-285
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• 2009

This paper has described about preparation of $Zr^{4+}$ affinity column based on the poly(styrene-co- gly-cidyl methacrylate) prepared by emulsion polymerization of styrene and glycidyl methacrylate in order to isolate phosphopeptide. The $Zr^{4+}$ ions were introduced after the phophonation of an epoxy group on polymeric microspheres. The successful preparation of $Zr^{4+}$-immobilized polymeric microsphere stationary phase was confirmed through Fourier transform infrared spectra, optical microscopy, scanning electron microscopy, X-ray photoelectron spectra and inductively coupled plasma-atomic emission spectrometer. The separation efficiency for $Zr^{4+}$ affinity column prepared by slurry packing was tested to phosphonated casein and dephosphonated casein. The resolution time (min) of the phosphonated casein was higher than that of dephosphated casein for $Zr^{4+}$ affinity polymeric microsphere by liquid chromatography. This $Zr^{4+}$ affinity column can be used for isolation of phosphonated casein from casein using liquid chromatography.

• Journal of Sensor Science and Technology
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• v.22 no.2
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• pp.144-149
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• 2013

In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated $2{\mu}m$ of pore-depth by two-step anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.

• Journal of Biosystems Engineering
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• v.39 no.2
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• pp.115-121
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• 2014

Background: Frequent outbreaks of foodborne illness have been increased awareness of food safety. CDC estimates that each year roughly 48 million people gets sick, 128,000 are hospitalized and 3,000 die of foodborne diseases in US. In Korea, 6,058 were hospitalized and 266 incidents were reported in 2012. It is required to develop rapid methods to identify hazard substances in food products for protecting and maintaining safety of the public health. However, conventional methods for pathogens detection and identification involve prolonged multiple enrichment steps. Purpose: This review aims to provide information on biosensors to detect pathogens in food products to enhance food safety. Results: Foodborne outbreaks continue to occur and outbreaks from various food sources have increased the need for simple, rapid, and sensitive methods to detect foodborne pathogens. Conventional methods for foodborne pathogens detection require tremendous amount of labor and time. Biosensors have drawn attentions in recent years because of their ability to detect analytes sensitively and rapidly. Principles along with their advantages and disadvantages of a variety of food safety biosensors including fiber optic biosensor, impedimetric biosensor, surface Plasmon resonance biosensor, and nano biosensor were explained. Also, future trends for the food safety biosensors were discussed.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.497-498
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• 2008

An integrated Mach-Zehnder interferometer for biosensor applications was designed and fabricated. To implement the optimum biosensor a rib waveguide must have single mode operation and high sensitivity. The proposed Mach-Zehnder interferometer was fabricated based on these design rules, and its feasibility is confirmed by ethanol detection experiment in the real-time measurement system operating at 632.8 nm.

• Applied Chemistry for Engineering
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• v.24 no.2
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• pp.113-120
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• 2013

Due to human life expectancy of the recent development of medical technology recently, it leads to increase the desire for improving the quality of human life, and grow health concerns and needs. Therefore, in order to prevent the occurrence of disease and to check up a disease quickly, research on the development of a biosensor has been actively processed. One of them, the nano-carbon materials, are very suitable for manufacturing biosensor due to their excellent electrical/mechanical properties. In this review, we introduced the recent studies about preparation methods of carbon electrodes using the carbon nano-materials for biosensors as well as its technological applications.