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Effect of Extenders with TCG and DMSO on the Viability of Rabbit Sperm

  • Eo, Yeol;Kim, Sang Hwan;Bang, Seong-Gyu;Oh, Min-Gee;Park, Chan-Hee;Yoon, Jong Taek
    • 한국동물생명공학회지
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    • 제34권2호
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    • pp.100-105
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    • 2019
  • The purpose of this study was to evaluate the effect of addition of ethylene glycol, glycerol and sucrose to TCG (Tris, Citric Acid, Glucose, Egg Yolk) and DMSO Frozen. The extender containing Egg yolk concentration (10%, 20%) affects viability and acrosome morphology of rabbit sperm. Sperm viability was then assessed for the freezing extenders TCGD (Tris + Citricacid + Glucose + DMSO), TCGED (Tris + Citricacid + Glucose + Egg yolk + DMSO), TCGGD (Tris + Citricacid + Glucose + Glycerol + DMSO) and TCGSD Tris + Citricacid + Glucose + Sucrose + DMSO) during thawing at 38℃. for 20 seconds, respectively. TCG + 10% egg yolk (viability: 77.0 ± 0.8, NAI: 73.3 ± 0.9) was significantly (sperm viability and normal acrosome interaction (NAI)) higher than TCG + 20% egg yolk (70.7 ± 1.1, 70.0 ± 0.9) in the sperm normalcy analysis according to the yolk concentration. TCGGD (53.4 ± 0.1, 62.3 ± 0.4), TCGSD (61.3 ± 0.0, 67.1 ± 0.1) sperm viability and normal acrosome interaction (NAI) in frozen spermatozoa are TCGD (46.4 ± 2.8 and 56.3 ± 1. 4) and TCGED (23.0 ± 1.1 and 54.6 ± 1.4) extenders was thawed at 38℃ for 20 seconds. According to the results from each frozen bulking agent, sperm membrane integrity by hypotonic swelling test (HOST) analysis in TCGGD (59.8 ± 0.7), TCGSD (59.3 ± 0.5) was significantly high compared to other experimental groups (p < 0.05). In conclusion, these results suggested that TCGGD and TCGSD extenders enhance survivability of rabbit sperm after frozen-thawing.

TES 보존액이 미니돼지 동결 융해 정자의 생존성 및 성상에 미치는 영향 (Effect of TES Extender on Sperm Characteristics and Viability of Frozen Semen in Miniature Pig)

  • 김상환;강현아;이명섭;서강석;윤종택
    • 한국수정란이식학회지
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    • 제27권1호
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    • pp.45-50
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    • 2012
  • The objective of this study is to estimate the effect of adding TES to LEY and FGE freezing extender for the sperm viability, acrosomal morphology and DNA fragmentation from miniature pig sperm, we evaluated sperm characteristics in TFGE, TLE and LEY with various thawing condition ($37^{\circ}C$ for 20 sec, 45 sec and $75^{\circ}C$ for 5 sec, respectively), and in different concentration of glycerol at 1%, 1.5%, 3%. The sperm viability and normal acrosome intact(NAI) in TFGE (Viability : $60.3{\pm}2.4$, NAI : $58.6{\pm}2.2%$), TLE ($61.3{\pm}2.4$, $62.2{\pm}2.2%$) extender significantly(p<0.05) increased than that in LEY ($50.2{\pm}2.4$, $54.5{\pm}2.2%$) extender thawed at $75^{\circ}C$ for 5 sec. According to the results from glycerol concentration, the viability and NAI of miniature pig sperm in 1.5% glycerol TLE ($66.1{\pm}3.2$, $66.2{\pm}1.0%$) was highest among the experimental groups. In accordance with this, DNA fragmentation rates was the lowest in TLE ($43.3{\pm}0.5%$) while that in LEY ($63.5{\pm}2.3%$) is the highest. Therefore, these results suggest that TLE extender method for freezing- thawing of miniature pig sperm increased the viability after thawing.

인영기구맥(人迎氣口脈)에 대(對)한 내경(內經)과 후대(後代) 의가설(醫家說)과의 비교(比較) 연구(硏究) (study of comparison between Nae-gyung(內經) and later physian's theory on In-yeong-gi-go Maek(人迎氣口脈))

  • 김태은;김태희
    • 대한한방내과학회지
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    • 제15권1호
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    • pp.152-164
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    • 1994
  • In Nai-Gyung(內經), it is said that In-Yeong(人迎) means In- Yeong Maek(人迎脈), Gi-Gu m- eans Tson-Gu(寸口) or Maek- Gu(脈口), In-yeong, artery in the side of neck, is located before Yeong-Geun(瓔筋), called as Jok-Yang-Myung-Maek(足陽明脈),so the part of In-Yeong-Maek means In-Yeong-Hyul(人迎穴) of Jok-Yang-Myung- Wi-Gyung(足陽明胃經) showing the artery in the side of neck. The part of Tson-Gu-Maek is that of artery in Yo-Gol(橈骨), beating source of Soo-Tae-Eum-Maek (手太陰脈) Of In-Yeong-Gi-Gu-Maek, In-Yeong-Maek mainly consists of Yang(陽), Gi-Gu-Maek of Eum(陰), so In-Yeung means physical disease, short of extra Eum component. They said that if In-Yeong-Maek was more than Gi-Gu-Maek, it meant physical injury, while Gi-Gu-Maek was more than In- Yeo-ng-Maek, it meant internal injury. In-Yeong-Gi-Gu-Maek is the one to distinguish the external and internal, the inside and outside, Eum and Yang, but there is no definite classification method to distinguish it, also it is difficult to grasp the comparison of 1sung(1盛), 2sung(2盛), 3sung(3盛), interrelation with 12-Gyung-Rak(l2經絡), change of maek phases, so necessary to set up the clear definition for In-Yeong-Gi-Gu-Maek. For the two theories as to In-Yeong-Gi-Gu-Maek according to Nai-Gyung, the one is to diagnose the maek by comparing the In-Yeong-Hyul with Tson-Gu of Soo-Tae-Eum-Maek as Gi-Gu in the both sides of neck part, he other is to divide the left and right of Gi-Gu-Maek in to In-Yeong and Gi-Gu afterwards, but today it is difficult to compare and explain the medical theory afterwards owing to insufficient consideration of In-Yeong-Gi-Gu-Maek at Nai-Gyung. The diagnosis of In-Yeong-Gi-Gu-Maek at Nai-Gyung to distinguish the surplus and shortage of Eum and Yang up to now since Nai-Gyung has so important and diagnostic value as to grasp the exact meaning. Herewith, this researcher com-pared Nai-Gyung and medical theory afterwards, reported it to consider the fixed position and changing process of viscera and entrails arrangement of literatures introducing In-Yeong-Gi-Gu-Maek, examining the change of maek phases for normal maek and a-bnormal maek of In-Yeong-Gi-Gu-Maek, considering the 1 sung, 2 sung, 3 sung maek phases in In-Yeong-Gi- Gu-Maek. According to the above results, the conclusion was reached as follows. 1. In-Yeong as the outside indicates external disease(外感), showing the surplus and shortage of Yang symptom by having Boo-Maek(浮脈) as Py-ung-Maek(平脈), Gi-Gu as the inside indicates internal disease(內傷), showing the surplus and shortage of Eum symptom by having Chim-Maek(沈脈) as P-yung-Maek(平脈). 2. In Pyung-Maek of In- Yeong-Maek as Boo-Maek, g-radual sinking of more floated changing maek because of disease means the improvement of di-sease, in Pyung-Maek of Gi- Gu-Maek as Chim- Maek, gradual floating of more sunken changing maek because of disease means the improvement of disease. 3. They said that disease of Jok-Gyung-Rak(足經絡) is cha-nged to that of Soo-Kyung-Rak(手經絡) when Jo-Maek(躁脈) appears whether In-Yeong-Maek or Gi-Gu-Maek 4. With the exemples of 1 sung 2 sung 3 sung it was porned with the relation of Pyo-Ri-Soo-Jok(表裏手足). Therfore I can guess that this fact is a moment explained the Bu-You-Sa-Kyung(部有四經) mentioned in Nan-Gyung-18-Nan(難經 第18難). 5. I think that In-Yeong and Gi-Gu, as a diagnosis method which distinguish between the inside indicates internal disease and the outside indicates external disease, is required to study further researches.

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Development of Startup Ecosystem in Vietnam in the context of the Fourth Industrial Revolution

  • Nguyen, Quoc Cuong;Tran, Thi Huyen;Kwon, HyukDong
    • International journal of advanced smart convergence
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    • 제9권2호
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    • pp.76-83
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    • 2020
  • The fourth industrial revolution (Industry 4.0) will generate great benefits and big challenges in equal measure. Industry 4.0 has an impact on context, technology and business as long as it transforms design, production systems and products themselves. Industry 4.0 has also created many opportunities and challenges for startups to improve their business efficiency. The reality has shown that the industry 4.0 has begun to strong impacts of various levels on many areas of Vietnam's economy. In such context, it is necessary to implement sufficient and thorough research and evaluation of many issues of startups and innovation and to consider suitable solutions and effective ways to take advantages of opportunities that the Industry 4.0 brings to Vietnam. This paper proposes the solution for supporting and promoting to develope of innovative start-up enterprise in accordance with the conditions and circumstances of Vietnam in the context of Industry 4.0.

"내경(內經)" 중심으로 경락(經絡)과 피부(皮膚)에 관한 연구(硏究) (The mutation research which with problem of the Spleen and the Stomach appears in skin care)

  • 김명주;전현정
    • 대한한의정보학회지
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    • 제14권2호
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    • pp.11-20
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    • 2008
  • This thesis' "Hwang Jae Nai Kyung(黃帝內經)", it is one among Uihak5kyung(醫學五經), is originally total of 18 volumes, the first half of 9 volumes are Somun(素問) and the latter half of the others are Youngchu(靈樞). Also, it is best of old book and literature integrating process of Chinese's medicine. Somun(素問) is comprehensive and extensive principles from fundamental problem of medical science related etiology, pathology, physiology to such parts of preventive medicine as regimen, recuperation. Moreover, therapeutic part of diagnosis and remedy, practice of acupuncture and moxibustion is recorded to Youngchu(靈樞). In the "Hwang Jae Nai Kyung(黃帝內經)", basic system takes precautions and medical cure for disease by approaching not method analyzing body but fundamentals of oriental medicine observed as organizing relation. For the purpose of mixing up beauty based on Buddhism of books, we shall pursuit notion of beauty treatment to method improving health because period flowing is changed to real well-being culture chasing outside beauty and healthy life. Then, to come close for part of oriental medicine and cosmetology, we shall present method of meridian pathways and understand device for cosmetic improvement by analyzing relation to 12 of pathways, the five viscera and the six entrails & skin.

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