• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.77.1-77
• /
• 2003

We have isolated a full-length cDNA clone, CaCDPK4 encoding a typical calcium-dependent protein kinase (CDPK) from hot pepper cDNA library. Genomic southern blot analysis showed that it belongs to a multigene family, but represents a single copy gone in hot pepper genome. RNA expression pattern of this gene revealed that it is induced by infiltration of Xanthomonas axonopodis pv. glycines Bra into hot pepper leaves but not by water deficit stress. However, high salt treatment of NaCl (0.4 M) solution to hot pepper plants strongly induced CaCDPK4 gene. In addition, this gene is weakly responsive to the exogenous application of salicylic acid or ethephon. Biochemical study of the GST-CaCDPK4 recominant protein showed that it autophosphorylates in vitro and the presence of EGTA, a calcium chelater, eliminates the kinase activity of the recombinant protein. As a way to identify the in vivo function of CaCDPK4 in plants, VIGS (Virus-Induced Gene Silencing) was employed. Agrobacterium-mediated TRV silencing construct containing the kinase and calmodulin domain of CaCDPK4 resulted in cell death of Nicotiana benthamiana plants. A highly homologous H benthamiana CDPK gene, NbCDPK5, to CaCDPK4 was cloned from N. benthamiana cDNA library. VIGS of NbCDPK5 also resulted in cell death. The molecular characterization of this cell death phenotype is being under investigation.

• Journal of Environmental Science International
• /
• v.17 no.4
• /
• pp.439-449
• /
• 2008

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.11
• /
• pp.1622-1629
• /
• 2013

The objective of this study was to develop a new method to separate phosvitin from egg yolk without using organic solvents. Phosvitin was extracted from yolk granules using 10% NaCl or 10% $(NH_4)_2SO_4$ (final concentration) and then treated with heat to precipitate the lipoproteins from the extracted solution. The optimal pH for the phosvitin extraction from yolk granules was determined, and the iron-binding ability of the extracted phosvitin (final product) was tested. Adding 10% $(NH_4)_2SO_4$ disrupted the granules, and the subsequent thermal treatment at $90^{\circ}C$ for 1 h precipitated low density and high density lipoproteins, which enabled separation of phosvitin by centrifugation. The phosvitin concentration in the extract was significantly higher when the pH of the solution was adjusted to pH ${\geq}9$. The purity and recovery rate of phosvitin at the end of the separation process were approximately 78% and 56%, respectively. The separated phosvitin was confirmed to have ferrous and ferric iron binding ability. The advantages of this new method compared with the traditional methods include no organic solvents and high-priced equipment are needed for the separation. Also, this method is more environment and consumer friendly than that of the traditional methods.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.25 no.5
• /
• pp.934-944
• /
• 2011

This study was designed to investigate the effects of Kyungohkgo to improve aerobic capacity and eliminate exercise-induced fatigue in high school soccer players. Twenty four subjects were participated and randomly assigned into two groups [KG, Kyungohkgo group (n=12); PG, Placebo control group (n=12)]. Two groups were completed treadmill exercise protocol using graded exercise test at before and after experimental treatment of 4 weeks. The $VO_2$max and endurance time were measured by gas analysis and heart rate (HR) was measured by polar system at pre, post 0, post 5, post 15, post 30 and post 60 minutes. Blood samples were collected to analyze blood components. 1. The $VO_2$max was significant increased in the group of after intake Kyungohkgo compared to the group of after intake placebo (p<.05). 2. The HR was significant decreased in the group of after intake Kyungohkgo compared to the group of after intake placebo during recovery time at post 5 mins(p<.05), 30 mins(p<.01), 60 mins(p<.01). 3. Weight, body mass index, percent body fat, anaerobic threshold, endurance time, blood lactate concentrate, lactate dyhydrogenase, creatine kinase, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, other energy sources(total-cholesterol, triglyceride, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, creatinine) and electrolyte (Na, K, Cl) were shown no significant differences between groups. These results suggested that Kyungohkgo can be used as ergogenic aids to improve aerobic capacity and eliminate exercise-induced fatigue.

• Plant Biotechnology Reports
• /
• v.4 no.2
• /
• pp.165-172
• /
• 2010

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1.5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of ${\beta}$-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.

• Journal of the Korean Institute of Gas
• /
• v.5 no.1
• /
• pp.52-59
• /
• 2001

Factors to cause accidents were investigated through a systematic analysis of leakage accidents in welded LP-gas cylinder between 199 and 1999. As a result It was showed that most of leakage accident occurred at the pin-hole due to the localized corrosion on welded zone in cylinder. Accordingly, in this paper, we suggested that the problems of heat treatment condition in two-piece welded cylinder and substitute proposal for preventing the tearing of T zone in three-piece welded cylinder. Furthermore, corrosion rate on the weldment and base metal of welded LP-gas cylinder was evaluated using the electrochemical tests in acid rain and $3.5\%NaCl$ solution, Also, the poor adhesion and defects of film which was produced by electrostatic powder spray painting, while the LP-gas cylinder was manufactured, were analyzed using SEM(Scanning Electron Microscopy). And we proposed a optimum coating thickness of the cylinder.

• Journal of Oriental Neuropsychiatry
• /
• v.4 no.1
• /
• pp.99-119
• /
• 1993

In order to compare and examine the effect the effect of Kuibitang, Kamiondarmtang and Kuibiondarmtang against stress, I induced immobilization stress by binding rats for 200mins under a little anesthetized with ether. I classified as normal group, control group which administrated 0.9% NaCl solution, sample A group which administrated Kuibitang, sample B group which administrated Kamiondarmtang and sample C group which administrated Kuibiondarmtang. I got some conclusions by measuring amounts of norepinephrine, epinephrine, triiodothyronine($T_3$) and thyroxine($T_4$) in 1, 3 and 5 days, respectively. 1. Norepinephrine showed high meaningful decrease after 1. 3 and 5 days in sample A group. It showed little decrease in sample B group. It showed a little meaningful decrease after 1 day and high meaningful decrease after 3 and 5 days in sample C group. 2. Epinephrine showed high meaningful decrease after 3 and 5 days in sample A group. It showed little decrease in sample B group. It showed a little meaningful decrease after 3 days and high meaningful decrese after 5 days in sample C group. 3. Triiodothyronine($T_3$) inclined to approach normal value in sample A, B, C group in comparision wit control group but didn't have high meaning. 4. Thyroxine($T_4$) inclined to approach normal va1ue in sample A, B, C group in comparision with contrl group but didn't have high meaning. As I consider the effect of experiments, I think that Kuibitang and Kuibiondarmtang have some effects against stress and it is right to apply to the treatment of stress.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.12
• /
• pp.1916-1924
• /
• 2019

Outbreaks of staphylococcal food poisoning (SFP) causing serious human diseases and economic losses have been reported globally. Furthermore, the spread of Staphylococcus aureus with increased resistance to multiple antimicrobial agents has become a major concern in the food industries and medicine. Here, we isolated an endolysin LysSAP8, as one of the peptidoglycan hydrolases, derived from the bacteriophage SAP8 infecting S. aureus. This endolysin was tagged with a 6×His at the C-terminal of the target protein and purified using affinity chromatography. LysSAP8 demonstrated lytic activity against a broad spectrum of bacteria, which included a majority of the staphylococcal strains tested in this study as well as the methicillin-resistant S. aureus (MRSA); however, no such activity was observed against other gram-positive or gram-negative bacteria. Additionally, LysSAP8 could maintain bactericidal activity until 0.1 nM working concentration and after heat treatment at 37℃ for 30 min. The ability of LysSAP8 to lyse cells under varying conditions of temperature (4-43℃), pH (3-9), and NaCl concentrations (0-1,000 mM), and divalent metal ions (Ca²⁺, Co²⁺, Cu²⁺, Mg²⁺, Mn²⁺, Hg²⁺, and Zn²⁺) was examined. At the optimized condition, LysSAP8 could disrupt approximately 3.46 log CFU/ml of the planktonic cells in their exponential phase of growth within 30 min. In this study, we have suggested that LysSAP8 could be a potent alternative as a biocontrol agent that can be used to combat MRSA.

• Food Science and Biotechnology
• /
• v.16 no.5
• /
• pp.715-721
• /
• 2007

In this study, the sphericity of calcium alginate gel beads was optimized using response surface methodology. The optimum conditions for bead sphericity were a concentration of 2.24% sodium alginate, a flow rate of 0.059 mL/sec for the sodium alginate solution, and a 459 rpm rotation for the calcium chloride solution. The predicted and experimental bead sphericities under the optimum conditions were 94.5 and 96.7%, respectively, showing close agreement. We also investigated the processing condition effects for the physical properties of the optimized calcium alginate gel beads. Immersion in hot water slightly decreased bead size and rupture strength. NaCl treatment increased bead size and decreased rupture strength. While the pH of the calcium chloride solution had little effect on bead sphericity, the bead sizes and gel strengths decreased with longer times in each pH solution. The beads coated with pectin and glucomannan showed no significant changes in sphericity, but their sizes decreased with time. The coated beads showed higher rupture strengths than the uncoated beads.

• Membrane and Water Treatment
• /
• v.8 no.3
• /
• pp.225-244
• /
• 2017

A novel thin-film nanocomposite (TFN) reverse osmosis (RO)/non-woven fabric (NWF) membrane was prepared by adding zinc oxide (ZnO) nanospheres ($30{\pm}10nm$) during the interfacial polymerization process of m-phenylenediamine (MPD) and trimesoyl chloride (TMC) on self-made polysulfone (PSF) membrane/polyester (PET) non-woven fabric support. The improved performance of TFN RO membrane was verified in terms of water contact angle (WCA), water flux, salt rejection, antifouling properties and chlorine resistance. The results showed that the WCA value of TFN RO surface had a continuous decrease with the increasing of ZnO content in MPD aqueous solution. The water flux of composite TFN RO membranes acquired a remarkable increase with a stable high solute rejection (94.5 %) in $1g{\cdot}L^{-1}$ NaCl aqueous solution under the optimized addition amount of ZnO (1 wt%). The continuous testing of membrane separation performance after the immersion in sodium hypochlorite solution indicated that the introduction of ZnO nanospheres also dramatically enhanced the antifouling properties and the chlorine resistance of composite RO membranes.