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Role of Intracellular Taurine in Monensin-induced $Na^+,\;Ca^{++}$ Accumulation and Mechanical Dysfunction in Isolated Rat Hearts

  • Kim, Young-Hoon;Park, Jong-Wan;Kim, Myung-Suk
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.5
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    • pp.537-546
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    • 1997
  • It has been postulated that the intracellular taurine is co-transported with $Na^+$down a concentration gradient and prevents the intracellular accumulation of sodium. It is therefore, expected that an elevated level of intracellular taurine prevents the sodium-promoted calcium influx to protect the cellular damages associated with sodium and calcium overload. In the present study, we evaluated the effects of intra- and extracellular taurine on the myocardial $Na^+$and$Ca^{++}$ contents and the cardiac functions in isolated rat hearts which were loaded with sodium by monensin, a $Na^+-ionophore$. Monensin caused a dose-dependent increase in intracellular $Na^+$ accompanied with a subsequent increase in intracellular $Ca^{++}$ and a mechanical dysfunction. In this monensin-treated heart, myocardial taurine content was decreased with a concomittent increase in the release of taurine. The monensin-induced increases in intracellular $Na^+$, $Ca^{++}$ and depression of cardiac function were prevented in the hearts of which taurine content had been increased by high-taurine diet. Conversely, in the hearts of which taurine concentration gradient had been decreased by addition of taurine in the perfusate, the monensin-induced increases in $Na^+$, $Ca^{++}$ and functional depression were accelerated. These results suggest that taurine, depending on the intra-extracellular concentration gradient, can affect intracellular sodium and calcium concentrations, and that an increased intracellular taurine may play a role in protection of myocardial dysfunction associated with the sodium and calcium overload.

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Effect of Different Conditions of Sodium Chloride Treatment on the Characteristics of Kenaf Fiber-Epoxy Composite Board

  • SETYAYUNITA, Tamaryska;WIDYORINI, Ragil;MARSOEM, Sri Nugroho;IRAWATI, Denny
    • Journal of the Korean Wood Science and Technology
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    • v.50 no.2
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    • pp.93-103
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    • 2022
  • Currently, biofibers are used as a reinforcement in polymer composites for structural elements and construction materials instead of the synthetic fibers which cause environmental problems and are expensive. One of the chemicals with a pH close to neutral that can be potentially used as a modified fiber material is sodium chloride (NaCl). Therefore, this study aims to investigate the characteristics of a composite board made from NaCl-treated kenaf fiber. A completely randomized design method was used with consideration of two factors: the content of NaCl in the treatment solution (1 wt%, 3 wt%, and 5 wt%) and the duration of immersion of fibers in the solution (1 h, 2 h, and 3 h). The NaCl treatment was conducted by soaking the fibers in the solution for different durations. The fibers were then rinsed with water until the pH of the water reached 7 and subsequently dried inside an oven at 80℃ for 6 h. Kenaf fiber and epoxy were mixed manually with the total loading of 20 wt% based on the dry weight of the fiber. Physical and mechanical properties of the fibers were then evaluated based on JIS A 5908 particleboard standards. The results showed that increasing NaCl content in the fiber treatment solution can increase the physical and mechanical properties of the composite board. The properties of fibers treated with 5 wt% NaCl for 3 h were superior with a modulus of elasticity of 2.085 GPa, modulus of rupture of 19.77 MPa, internal bonding of 1.8 MPa, thickness swelling of 3%, and water absorption of 10.9%. The contact angle of untreated kenaf fibers was 104°, which increased to 80° and 73° on treatment with 1 wt% and 5 wt% NaCl for 3 h, respectively.

Fabrication and Crystallization Behavior of BNN Thin Films by H-MOD Process

  • Lou, Jun-Hui;Lee, Dong-Gun;Lee, Hee-Young;Lee, Joon-Hyung;Cho, Sang-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07b
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    • pp.739-743
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    • 2003
  • [ $Ba_2NaNb_5O_{15}$ ], hereafter BNN, thin films are attractive candidates for nonvolatile memory and electro-optic devices. In the present work, thin films that have different contents of Ba, Nb and Na have been prepared by H-MOD technique on silicon and Pt substrates. XRD and SEM were used to investigate the phase evolution behavior and the microstructure of the films. It was found that the films of about 500nm thick were crack-free and uniform in microstructure. Nb content strongly influenced the phase formation of the films, where unwanted phases were always formed at the stoichiometric BNN composition. However, the unwanted phases decreased with the increase of excess Nb content, and the single phase (tetragonal tungsten bronze structure) BNN thin film was obtained when the niobium content reached some point. From this study, the sub-solidus phase diagram below $850^{\circ}C$ for $BaO-Na_2O-Nb_2O_5$ ternary system is proposed.

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Desalinization Characteristics after Reclamation of Tidal Flat on the Western Coast of Korea (서해안 간척지 토양의 탈염특성)

  • 민병미;김준호
    • The Korean Journal of Ecology
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    • v.20 no.4
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    • pp.275-283
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    • 1997
  • Vertical and temporal characteristics of desalinized reclaimed soil were analyzed from reclaimed coastal land on the western coast of Korea. Of the vertical changes during desalting, pH valuse were the lowest at the topsoil without regard to reclaimation time. The content of C1 were designated as the early period (the first 2-4 years) which decreased exponentially and the later period(the last 5-7 years) which was almost constant, from top to down. In temporal changes of the soil attributes, pH values increased for 5 years and decreased at 6 year after reclamation. Chlorine leaches more rapidly than Na does, K and Ca are constant but Mg increases as time elapsed after reclamation. Sometimes the content of Ca and K in the reclaimed soil are of higher concentration than that of the seawater after reclamation. During desallinization as exemplified by decreasing EC of the soil, Cl and Na are rapidly leached, but K, Ca and Mg are somewhat enhanced. The ration of Na/Cl in the soil equals 1 when the EC registers 5 mmho and then increases dramatically as the EC decreases. Rapid leaching of $Cl^{-}$ elicits an increasing pH valus. The electrostatic balance of the soil is achived by replacement of $Cl^{-}$ with $OH^{-}$ until stationary or until a decreasing pH value is reached again.

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The Electrochemical Properties and Mechanism of Formation of Anodic Oxide Films on Mg-Al Alloys

  • Kim, Seong-Jong;Okido, Masazumi
    • Bulletin of the Korean Chemical Society
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    • v.24 no.7
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    • pp.975-980
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    • 2003
  • The electrochemical properties and the mechanism of formation of anodic oxide films on Mg alloys containing 0-15 mass% Al, when anodized in NaOH solution, were investigated by focusing on the effects of anodizing potential, Al content, and anodizing time. The intensity ratio of Mg(OH)₂ in the XRD analysis decreased with increasing applied potential, while that of MgO increased. Mg(OH)₂ was barely detected at 80 V, while MgO was readily detected. The anti-corrosion properties of anodized specimens at each constant potential were better than those of non-anodized specimens. The specimen anodized at an applied potential of 3 V had the best anti-corrosion property. The intensity ratio of the β phase increased with aluminum content in Mg-Al alloys. During anodizing, the active dissolution reaction occurred preferentially in β phase until about 4 min, and then the current density increased gradually until 7 min. The dissolution reaction progressed in α phase, which had a lower Al content. In the anodic polarization test in 0.017 mol·$dm^{-3}$ NaCl and 0.1 mol·$dm^{-3}$ Na₂SO₄ at 298 K, the current density of Mg-15 mass% Al alloy anodized for 10 min increased, since the anodic film that forms on the α phase is a non-compacted film. The anodic film on the α phase at 30 min was a compact film as compared with that at 10 min.

Effects of Processing Conditions on Some Characteristics of Dongchimi Juice

  • Kim, Dong-Hee;Chum, Yun-Kee;Kim, Woo-Jung
    • Preventive Nutrition and Food Science
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    • v.1 no.1
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    • pp.46-52
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    • 1996
  • Effective method for the preparation of Dongchimi juice was developed by addition of NaCi, sucrose and bydrolytic enzymes before fermentation and addition on Dongchimi juice during fermentation. The radish was ground and suspended in water(1:1, w/v) with addition of spices(garlic, green onion and ginger) followed by fermentation at $25^{\circ}C$. The addition of 2% NaCi and 0.5~2.0% sucrose resulted in significant increase of solid content and it was also improved by the addition of polysaccharide hydrolyzing enzyme during fermentation. When the fermented juices of pH 5.4 of 4.4 were added by 15% of total weight before(pH 5.4 juice)and during (pH 4.4 juice) fermentation, a significant increase in solid content after 24hrs of fermentation was resulted. The combined method of addition of 2% NaCI, 1.0% sucrode, 0.1% Viscozyme and 10% of fermented juice of pH 5.4 and 4.$ before and during fermentation improved solid content, reducing sugar, color and showed little effect on viscosity. The organoleptic characteristics were also improved by the combined method.

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The Control Efficacy of Sodium Hypochlorite against Violet Root Rot Caused by Helicobasidium mompa in Apple

  • Lee, Sung-Hee;Shin, Hyunman;Lee, Hyok-In;Lee, Seonghee
    • The Plant Pathology Journal
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    • v.38 no.5
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    • pp.513-521
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    • 2022
  • Our study was carried out to determine the control efficacy of sodium hypochlorite (NaOCl) for violet root rot caused by Helicobasidium mompa in apple. The experiment was conducted in the farm located at Chungbuk province in South Korea from 2014 to 2016. When infected apple trees were treated at least two or three times with 31.25 and 62.5 ml/l available chlorine content in NaOCl, it greatly increased the rooting of rootstock, and restored the tree crown density by 44.4-60.5%. In addition, the number of commercial fruit setting was increased by 54.3-64.5%, and the total starch content in shoots was significantly higher than other non-treated apple trees. However, the untreated disease control and thiophanate-methyl WP treated trees showed the symptom of dieback. Therefore, our results indicate that the drenching treatment of NaOCl with 31.25-62.5 ml/l available chlorine content more than two times from late fall to early spring could effectively control the violet root rot and recover tree vigor up to 60%.

The Effect of Sodium Chloride on the Quality of Cheese and Upcoming Technologies for Manufacturing Reduced-Sodium Cheeses: A Review (Sodium Chloride가 치즈의 품질에 미치는 영향과 저염치즈 개발 기술: 총설)

  • Chon, Jung-Whan;Kim, Hyun-Sook;Kim, Dong-Hyeon;Kim, Hong-Seok;Song, Kwang-Young;Jeong, Dong-Gwan;Kim, Soo-Ki;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.33 no.1
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    • pp.47-57
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    • 2015
  • Sodium is an essential nutrient with very important functions, including regulation of the extracellular fluid volume and active transport of molecules across the cell membranes. Since high levels of dietary sodium are associated with a high prevalence of hypertension, prehypertension, and other adverse effects on health, many national and international health organizations (WHO, FAO, etc.) recommend that sodium intake should be significantly decreased. In developed nations, cheese products, from among many processed foods, can cause high salt intake. Hence, there is an urgent need to reduce the content of salt in cheese processing, using various substitutes of sodium chloride (NaCl). In general, salt (NaCl) has been used as a food preservative to limit and (or) kill the growth of foodborne pathogens and spoilage organisms by decreasing the water activity, and to improve texture and flavor. To maintain public health, the salt content in cheese should be decreased without influencing the physicochemical properties of cheese. Therefore, the objective of this review is to outline the upcoming technologies used to reduce the salt content in different types of cheese using various substitutes.

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Physicochemical Changes of Woody Charcoals Prepared by Different Carbonizing Temperature (탄화온도가 목탄의 물리·화학적 특성에 미치는 영향)

  • Jo, Tae-Su;Choi, Joon-Weon;Lee, Oh-Kyu
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.3
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    • pp.53-60
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    • 2007
  • Carbon content, properties of micro-pore, and chemical properties of the charcoal prepared from wood powder, wood fiber, and bark of Abies sibirica Ledeb at different temperatures were investigated. The yield of charcoal decreased with increasing the carbonization temperature. The yield of bark charcoal was higher than those of wood and wood fiber charcoal. The content ratio of carbon atom in the charcoal increased with increasing the carbonization temperature, whereas those of hydrogen and oxygen atom were decreased. Ash content of bark charcoal was also higher than those of wood and wood fiber charcoal. The specific surface area of wood and wood fiber charcoal was greater than that of bark charcoal. In all charcoal, the specific surface area and the volume of micro-pore were highest when the carbonization temperature was $600^{\circ}C$, however they tended to decrease when the temperature was reached to $800^{\circ}C$. For the functionality test of chemical groups on the charcoal surface, adsorption test have performed against acidic (HCl) and basic chemicals (NaOH, $Na_2CO_3$, and $NaHCO_3$). As carbonization temperature increased, adsorption amount of HCl increased, while adsorption amounts of NaOH, $Na_2CO_3$, and $NaHCO_3$ were decreased. The charcoal prepared at higher temperature showed basic properties, while the charcoals manufactured at lower temperature presented acidic properties. Therefore, it was considered that the carbonization temperature affected the pH of charcoal.

Effect of NaCl on Salt-tolerant Callus in Tobacco (내염성 담배 캘러스에 대한 NaCl의 효과)

  • 차현철
    • Journal of Plant Biology
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    • v.36 no.1
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    • pp.113-120
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    • 1993
  • Effects of various NaCl concentrations on salt-tolerant callus of tobacco were investigated. Selection of NaCl-tolerant (S) callus was conducted by subculturing Nicotiana tabacum cv. BY 4 callus in 200 mM NaCl-containing MS medium for more than 18 months. In spite of the long subculture period, characteristics of salt tolerance were maintained very stably. Significant differences were found in ion contents of each callus which was subcultured with treatment of various NaCl concentrations: Na+ and Cl- became higher but Mg2+, Ca2+ and K+ became lower with the increasing external salt contents. Therefore, the ratios of Na+/Ca2+ and Na+/K+ also increased resulting close to those of halophytic property. The contents of chlorophylls and carotenoids in S callus were estimated to 3.1 and 2.9 times more, respectively. than those of non-selected (NS) callus (control). The higher content of external NaCl tended to increase the amount of water soluble proteins and to decrease the amounts of the total sugars, reducing sugars and free amino acids. The activity of peroxidase was increased with higher contents of external NaCl in S callus, but it was maintained at a higher level than S callus at lower NaCl, followed by a subsequent decrease above 80 mM NaCl in NS callus. These results suggest that S callus may have a biological system converting energy source to efficient growth leading to reduction of the growth inhibition under stress environment.

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