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THE EFFECTIVENESS OF TWO SEDATIVE REGIMEN USED FOR CHILD PATIENT WHO FAILED TO ORAL CHLORAL HYDRATE (Chloral hydrate 경구 진정에 실패한 소아환자를 대상으로 사용한 두 가지 진정요법의 효과)

  • Lee, Sang-Min;Kim, Jong-Soo;Kim, Yong-Kee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.4
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    • pp.505-516
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    • 2000
  • Chloral hydrate is one of the most widely used sedative agents to control the difficult-to-treat young age group in the dental clinic. We are often frustrated to see the patient still awake and cry with agitation even after far more than the normal onset time of Chloral hydrate. In such a case, the patient has to be rescheduled for another sedation visit with different agents and/or routes which greatly disappoints the guardians. This study was designed to test the efficacy of one sedative regimen that can possibly help the clinician complete scheduled treatment without postponement. We have tried sleep induction with mixed gas of Enflurane(2vol%) and $N_2O(50%)-O_2(50%)$ for $60\sim120$ seconds to 35 patients of those who failed to respond properly to the dose(70mg/kg)of oral Chloral hydrate. The Result of this regimen was compare to those of two oral regimen of Chloral hydrate/Hydroxyzine and Chloral hydrate only Analyses of result on vital signs and behavior pattern were performed. The outcome of the study suggest that sleep induction by a short inhalation of low dose of $Enflurane/N_2O-O_2$ provide dentist with suitable condition for the completion of scheduled treatment in the patient who failed to oral Chloral hydrate. Evidence of adverse effect was not detected or reported during and/or after the procedures.

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Rumen fermentation and performance of Hanwoo steers fed total mixed ration with Korean rice wine residue

  • Jeong, Chang-Dae;Mamuad, Lovelia L.;Ko, Jong Youl;Sung, Ha Guyn;Park, Keun Kyu;Lee, Yoo Kyung;Lee, Sang-Suk
    • Journal of Animal Science and Technology
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    • v.58 no.1
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    • pp.4.1-4.7
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    • 2016
  • Background: This study was conducted to evaluate the effects of adding Korean rice wine residue (RWR) in total mixed ration (TMR) on in vitro ruminal fermentation and growth performance of growing Hanwoo steers. Methods: For in vitro fermentation, the experimental treatments were Control (Con: 0 % RWR + TMR), Treatment 1 (T1: 10 % RWR + TMR), and Treatment 2 (T2: 15 % RWR + TMR). The rumen fluid was collected from three Hanwoo steers and mixed with buffer solution, after which buffered rumen fluid was transferred into serum bottles containing 2 g dry matter (DM) of TMR added with or without RWR. The samples were then incubated for 0 h, 12 h, 24 h, or 48 h at $39^{\circ}C$ and 100 rpm. For the in vivo experiment, 27 Hanwoo steers (6 months old) with an average weight of $196{\pm}8.66kg$ were subjected to a 24-week feeding trial. The animals were randomly selected and equally distributed into three groups. After which the body weight, feed intake and blood characteristics of each group were investigated. Results: The pH of the treatments decreased significantly relative to the control during the 12 h of incubation. Total gas production and ammonia nitrogen ($NH_3-N$) was not affected by RWR addition. The total volatile fatty acid (VFA) was lower after 24 h of incubation but at other incubation times, the concentration was not affected by treatments. Feed cost was 8 % and 15 % lower in T1 and T2 compared to control. Blood alcohol was not detected and a significant increase in total weight gain and average daily gain were observed in Hanwoo steers fed with RWR. Conclusion: Overall, the results of this study suggest that TMR amended with 15 % RWR can be used as an alternative feed resource for ruminants to reduce feed cost.

Fragrance, Chemical Composition and Toxicity of the Essential Oil in Erect Bur-marigold (Bidens tripartita L.) (가막사리 (Bidens tripartita L.) 정유의 향취, 화학성분 및 세포독성)

  • Yun, Mi-Sun;Yeon, Bo-Ram;Cho, Hae-Me;Lee, Sa-Eun;Jhoo, Jin-Woo;Jung, Ji-Wook;Park, Yu-Hwa;Kim, Song-Mun
    • Korean Journal of Weed Science
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    • v.32 no.3
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    • pp.195-203
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    • 2012
  • The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil:17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were ${\alpha}$-phellandrene (22.50%), ${\alpha}$-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), ${\beta}$-pinene (35.43%), and ${\beta}$-cubebene (5.27%). The $IC_{50}$ value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.

Quantitative Analysis of Cystic Fluid Components in Cysticercus cellulosae (유구낭미충(有鉤囊尾蟲) 낭액(囊液)의 아미노산(酸) 및 유리지방산(遊離脂肪酸)의 정량분석(定量分析))

  • Moon, Joon;Chung, Myung-Sook;Joo, Kyoung-Hwan;Rim, Han-Jong
    • Journal of agricultural medicine and community health
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    • v.16 no.2
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    • pp.141-153
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    • 1991
  • Free amino acid(FAA), free fatty acid(FFA), and amino acid obtained by hydrolysis of protein components of cystic fluid(CF) of Cysticercus cellulosae in pig and man were analyzed. FFA was analyzed by gas chromatography using Varian model 2700, and flame ionization detector with 6 feet${\times}$1/4inch glass column. Flow rate of $N_2$ was 30 ml/min, $H_2$ was 30 ml/min, air was 350 ml/min respectively and chart speed was 1 cm/min. Amino acid was analyzed by high performance liquid chromatography using Waters model 441, and fluorescence detector at 338nm/425nm with column of amino acid analyzer. Buffer A of mobile phase was pH 3.05 and pH of buffer B was 9.6 respectively. The results obtained were as follows : Seven FFAs containing 12~18 carbons were detected : Saturated fatty acids were lauric acid ($C_{12}$), myristic acid($C_{14}$), palmitic acid($C_{16}$), Stearic acid($C_{18}$). Unsaturated fatty acids were oleic acid($C_{12}^{=1}$), linoleic acid($C_{12}^{=2}$), and one unidentified fatty acid was detected. Generally much more quantity of FFA was determined in CF obtained from pig than that from man. FFA of the largest quantity was palmitic acid; 0.078 mg/ml. Eighteen FAAs were detected and the largest quantity was alanine. Ouantity of alanine was 386 ug/ml in CF from pig 108 ug/ml in CF from man respectively. while histidine in CF from pig was 273 ug/ml, that from man was only 4.3 ug/ml. Eighteen amino acids were identified by hydrolysis of protein in CF from man. But, histidine was not identified in CF from pig. Amino from pig and ug/ml from man.

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Field Applicability of Scale Prevention Technologies for Drainage Holes (배수공 내 스케일 생성 방지 기술의 현장 적용성 평가)

  • Chu, Ickchan;Lee, Jonghwi;Kim, Hyungi;Kim, Kyungmin;Chun, Byungsik
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.9
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    • pp.45-51
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    • 2012
  • The calcium hydroxide$(Ca(OH)_2)$ which is the cement hydrate flowed into the tunnel by groundwater is reacted with microorganism in the soil, carbon dioxide$(CO_2)$ and the vehicle's exhaust gas$(SO_3)$. So its by-products are precipitated at the drainage pipe and these cause the drainage clogging. By this phenomenon, Degradation of water flow at the drainage system of the tunnel occurred and also pore water pressure is increased. Hence the acceleration of seepage and degradation of lining is occurred. The purpose of this study is to evaluate the field applicability of the Quantum Stick and Magnetic treatment in prevention of scale deposits at the Namsan ${\bigcirc}{\bigcirc}$ tunnel and the Zone ${\bigcirc}{\bigcirc}{\bigcirc}$ of subway. These technologies were installed into drainpipes with their performance monitored through occasional site visits. SEM and XRD were also performed on scale collected from these drainpipes. As a result, in case which factor technology is applied, scale creation is remarkably decreased and especially Quantum Stick treatment performing better than Magnetic treatment. Therefore, additional application of Quantum Stick or Magnetic treatment to the existing drainage is expected to decrease the drainage clogging of the drainage.

Computational Simulation of Hydrocarbon Adsorption in a Packed Column (탄화수소 흡착 컬럼의 전산모사 특성)

  • Yoo, Kyung-Seun;Lee, Su-Jung;Kim, Ji-Eun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.3
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    • pp.10-16
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    • 2020
  • Computational simulations of adsorption columns were carried out to investigate the removal characteristics of VOCs from a laundry shop. n-Decane was selected as the representative component among the VOCs emitted, and the activity of the adsorbents, such as activated carbon, was evaluated using commercial CFD code. The mathematical framework was composed of continuity and Navier-stokes equations, and the simulation was performed using the Matlab program. The adsorption isotherms of LDF, Freundlich, and Langmuir were evaluated, and the adsorption amount of the adsorption isotherms with the adsorption parameter was compared. The simulation was carried out using a particle porosity, dispersion coefficient, particle density, bed diameter, and bed length of 0.79, 42.4 ㎠/min, 485 g/L, 2.0 cm, and 2.5 cm, respectively. The effect of the gas velocity, dispersion coefficient, and voidage on the adsorption amount was compared in the Langmuir adsorption isotherm. The simulation was carried out in the velocity range of 50 to 200 cm/min, dispersion coefficient range of 100 to 400 ㎠/min, and particle porosity range of 0.66 to 0.79. The simulation results of activated carbon with benzene coincided with the Langmuir isotherm. Three types of adsorption isotherm were compared under similar conditions, and the simulation results showed the efficient adsorption condition for hydrocarbons.

The Study on Analysis Method and Performance Evaluation of Portable GC/MS (휴대용 GC/MS 성능검사 및 분석방법 연구)

  • Park, Yuonshin;Noh, Hyeran;Yang, Heeseon;Seok, Gwanseol
    • Journal of the Society of Disaster Information
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    • v.9 no.3
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    • pp.249-258
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    • 2013
  • Recieved Mar. 06, 2013 Revised Mar. 29, 2013 Accepted Sep. 11, 2013In this study, we evaluated the method performance and the optimum sample injection method of the portable GC/MS(HAPSITE) which were designed to operate on scene of chemical accidents. Chemicals used for performance test were vinyl chloride(VC), methyl chloride(MC), benzene(Bz), toluene(Tol). CRM(Certified Reference Material) Manufactured by KRISS(Korea Research Institute of Standards and Science) was used as the standard gas. The results showed that 1) Among three sample injection methods(Cylinder, Silico canister, and Bag), bag was identified as the most appropriate sample injection method. Bag material and capacity did not significantly affect the results. The most ideal reproducibility occurred at the 2-minute point of the purge time in the loop and tenax methods. 2) Performance evaluation of HAPSITE was conducted. Evaluation items were reproducibility, linearity, method detection limit, etc. Tenax concentration method was appropriate for larger molecules and Carbopack concentration method was suitable for smaller molecules.

Hydrogen Electrode Performance with PTFE Bonded Raney Nickel Catalyst for Alkaline Fuel Cell (라니 니켈 촉매에 대한 알칼리형 연료전지용 수소극의 전극특성)

  • Lee, Hong-Ki;Lee, Ju-Seong
    • Applied Chemistry for Engineering
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    • v.3 no.3
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    • pp.527-534
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    • 1992
  • Raney nickel was used as catalyst in the hydrogen electrode for an alkaline fuel cell. The hydrogen electrode manufactured with the Raney nickel catalyst which was sintered at $700^{\circ}C$ was found to have the highest electrode performance. Using the Raney nickel powder of average particle size $90{\AA}$ for the electrode, the current density which had been measured was $450mA/cm^2$ at $80^{\circ}C$ using 6N KOH solution as an electrolyte. The effects of PTFE addition were investigated with CO-chemisorption, polarization curves and Tafel slope. CO-chemisorption had shown the optimum value when the Raney nickel was mixed with 5wt% of PTFE, but from the current density and Tafel slope at porous Raney nickel electrode, the appropriate value of PTFE addition was 10wt%. Recommendable Ni and Al portion for Raney nickel was 60 : 40 and loading amount was $0.25g/cm^2$. Also the influence of pressing pressure for manufacturing catalytic layer and for junction with gas diffusion layer was examined. The morphology of catalyst surface was investigated with SEM. The influence of reactivation time and heat-treatment temperature were also studied.

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Determination of trace arsenic in seawater by flow injection-hydride generation inductively coupled plasma mass spectrometry (연속흐름주입-수소화물생성-유도결합플라스마 질량분석장치를 이용한 바닷물표준시료중의 극미량 비소분석방법의 확립)

  • Suh, Jung-Ki
    • Analytical Science and Technology
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    • v.21 no.4
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    • pp.316-325
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    • 2008
  • An inductively coupled plasma mass spectrometry (ICP-MS) instrument equipped with flow injection-hydride generation system was used for the determination of trace arsenic in seawater sample. The accuracy in this method was verified by the analysis of certified reference materials (CRM) of seawater (CASS-4, NASS-5). The analytical results agreed with certified value within the range of uncertainty. The expanded uncertainties for CASS-4 and NASS-5 in this experiment were ranged from 6.2% to 6.8% obtained from repeated analyses of the CRMs (n=5). The detection limit of $As^+$ (m/z=74.9216) in this method was confirmed about 0.01 ug/kg. Linearity obtained from calibration curve of arsenic was excellent ($R^2=1$). The detection at $As^+$ (m/z=74.9216) and $AsO^+$ (m/z=90.9165) by using oxygen reaction gas in DRC mode was compared. Sensitivity at $AsO^+$ (m/z=90.9165) was decreased about 25-fold, but the analytical results are the same that at $As^+$ (m/z=74.9216).

Effect of Soybean Meal and Soluble Starch on Biogenic Amine Production and Microbial Diversity Using In vitro Rumen Fermentation

  • Jeong, Chang-Dae;Mamuad, Lovelia L.;Kim, Seon-Ho;Choi, Yeon Jae;Soriano, Alvin P.;Cho, Kwang Keun;Jeon, Che-Ok;Lee, Sung Sil;Lee, Sang-Suk
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.50-57
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    • 2015
  • This study was conducted to investigate the effect of soybean meal (SM) and soluble starch (SS) on biogenic amine production and microbial diversity using in vitro ruminal fermentation. Treatments comprised of incubation of 2 g of mixture (expressed as 10 parts) containing different ratios of SM to SS as: 0:0, 10:0, 7:3, 5:5, 3:7, or 0:10. In vitro ruminal fermentation parameters were determined at 0, 12, 24, and 48 h of incubation while the biogenic amine and microbial diversity were determined at 48 h of incubation. Treatment with highest proportion of SM had higher (p<0.05) gas production than those with higher proportions of SS. Samples with higher proportion of SS resulted in lower pH than those with higher proportion of SM after 48 h of incubation. The largest change in $NH_3$-N concentration from 0 to 48 h was observed on all SM while the smallest was observed on exclusive SS. Similarly, exclusive SS had the lowest $NH_3$-N concentration among all groups after 24 h of incubation. Increasing methane ($CH_4$) concentrations were observed with time, and $CH_4$ concentrations were higher (p<0.05) with greater proportions of SM than SS. Balanced proportion of SM and SS had the highest (p<0.05) total volatile fatty acid (TVFA) while propionate was found highest in higher proportion of SS. Moreover, biogenic amine (BA) was higher (p<0.05) in samples containing greater proportions of SM. Histamines, amine index and total amines were highest in exclusive SM followed in sequence mixtures with increasing proportion of SS (and lowered proportion of SM) at 48 h of incubation. Nine dominant bands were identified by denaturing gradient gel electrophoresis (DGGE) and their identity ranged from 87% to 100% which were mostly isolated from rumen and feces. Bands R2 (uncultured bacterium clone RB-5E1) and R4 (uncultured rumen bacterium clone L7A_C10) bands were found in samples with higher proportions of SM while R3 (uncultured Firmicutes bacterium clone NI_52), R7 (Selenomonas sp. MCB2), R8 (Selenomonas ruminantium gene) and R9 (Selenomonas ruminantium strain LongY6) were found in samples with higher proportions of SS. Different feed ratios affect rumen fermentation in terms of pH, $NH_3$-N, $CH_4$, BA, volatile fatty acid and other metabolite concentrations and microbial diversity. Balanced protein and carbohydrate ratios are needed for rumen fermentation.