• 한국조경학회지
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• 제31권5호
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• pp.11-19
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• 2003

The main purpose of this study was to examine the application of the root system as a shore protection material through the measurement of the 개ot growth curve of Salix gracilisyla Miq. depending on the cutting size. As materials and methodology, nine group of cuttings were classified by the length(l0cm, 20cm, 40cm) and the diameter(0.72cmm$\pm$0,02, 0.58cm$\pm$0.02, 0.35cm$\pm$0.02), Each group was stuck to a flooding bed of sandy loam(Sand 60,36%, Silt 28%, Clay 11.64%) on 27th March 2001, After 65 growing days, the weight and length of the newly developed roots, shoots, and leaves were measured and analysed, This was repeated at 99, 129, 159, and 190 growing days. The major findings were as follows. The primary determinant of the root growth rate was on the weight of cutting, The secondary determinant was on the number of growing days. In addition, the dominant dimension of the cutting was the diameter rather than the length, The thicker cutting caused more rapid and stable growth however the longer cutting made the growth of the root slower and more unstable.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.60-60
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• 2020

To establish in vitro axillary bud culture conditions of Echinosophora koreensis Nakai, one of Korean endemic endangered species famous for beautiful flowers, we tested the influence of plant growth regulators (PGRs) in shooting and rooting stage from in vitro plants. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the media induced 2.5 to 3 shoots per bud during 4 weeks of culture. And media including 0.5 mg L^-1 thidiazuron (TDZ) produced 3 to 4 shoots per bud. However, zeatin and isopentenyl adenine (2-ip) were not successful to increase shoot number, and the combination treatments of BA with other PGRs were also not effective. Shoots were smaller than 2 cm in length, in most of the treatments. In rooting, naphthalene acetic acid (NAA) treatments in the range of 0.5 to 4.0 mg L^-1 appeared to increase rooting rate by 10% to 60% approximately when compared with the control but roots developed with callus clusters. Indole butyric acid (IBA) addition had little effect on rooting (below 10%), while some roots were longer than in NAA treatments and some shoots were longer on high IBA concentrations (4.0 to 8.0 mg L^-1). It is suggested that micropropagation is a highly applicable and promising to multiplication and conservation of rare and endangered endemic species.

• 한국유기농업학회지
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• 제24권3호
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• pp.511-524
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• 2016

이 연구는 도라지의 유기재배를 위하여 유기질 비료 시용이 도라지 뿌리의 생육특성 및 사포닌, 항산화 활성에 미치는 영향을 구명하고자 실시되었다. 3년근 도라지를 이식하기 전 기비로 화학비료, 유기질비료, 균배양체, 유박 및 퇴비를 시용한 결과, 뿌리의 길이는 화학비료와 유기질 비료 시용구에서 25.3 cm와 24.0 cm로 가장 길었으며, 직경에서도 화학비료 시용구에서 가장 굵은 26.6 mm 나타났다. 지근의 수는 유기질퇴비와 화학비료 시용구에서 가장 많은 20.0개와 17.0개로 조사되었으며, 수량과 관련성이 있는 생체중은 유기질비료 시용구에서 55.7 g으로 가장 높게 나타났다. Platycodin D의 함량은 327.4~373.8 mg/100 g의 범위로 유기질퇴비 시용구에서 높은 값이 나타났으며, 도라지의 총 polyphenol 및 flavonoid 함량은 유기질비료 시용구에서 각각 15.5, 15.3 mg/g 가장 높게 나타났다. 따라서 도라지의 유기재배시 유기질비료 시용구에서 수량성을 물론, 약리성 증대를 위한 사포닌 함량이 많은 지근의 수와 항산화 성분이 높은 것으로 나타났다.

• 한국초지조사료학회지
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• 제23권2호
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• pp.91-94
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• 2003

비교되는 포장조건에 따라 부리 분포 양상을 조사하기 위하여 Rhizotron법을 사용하였다. Lincoln 지역에서 토양 깊이에 따른 뿌리의 분포는 15∼30 cm 깊이에 대부분의 뿌리가 분포되어 있고, 0∼15 cm와 30 cm 이하에서는 분포가 적었다. 반면, Winchmore에서는 토양 깊이가 깊어질 수록 뿌리 수가 많아졌다. Mt John에서는 표토에 가까울 수록 뿌리 분포도가 더 높았으며, 시비량이 높고 관개를 한 곳이 부리 분포가 더 높았다. 모든 지역에서 토양 깊이에 따른 뿌리 수의 분포는 뿌리 길이와 같은 경향을 보여주었다. Tube법이 같은 장소에서 뿌리의 비교 연구와 장기간 무리 생장 연구를 하는데 좋은 방법임이 입증되었다.

• 한국작물학회지
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• 제34권2호
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• pp.147-154
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• 1989

일본형 수도, 통일형 수도 및 통일형 수도의 교배친 등을 공시하여 이의 종근으로부터 근단을 1cm로 절취한 분리근을 천전 등의 $\ulcorner$개량배지$\lrcorner$를 기본으로 하여 pH, 당농도, Casamino acid 농도 등을 달리한 oq지에 무균배양한 분리근의 생장 튿성을 비교하였던 바 얻어진 결과를 요약하면 다음과 같다. 1. 분리근의 근장은 27$^{\circ}C$구에서는 일본형품종과 다수형 품종간에 별차이는 없었으나 3$0^{\circ}C$구에서는 일본형 수도에 비교해서 통일형 품종의 신장이 현저히 양호하였다. 2. 분리근의 건물중은 27$^{\circ}C$구 및 3$0^{\circ}C$구 공히 일본형 품종에 비교해서 통일형 품종이 현저하게 무거운 것으로 인정되었다. 3. 일본형 품종과 통일형 품종간에 나타난 분리근의 건물중 차이는 주로 측근발육의 양부에 따른 것으로 특히 2~3cm길이의 특근수에 차이가 인정되었다. 4. Casamino acid 반응에서 일본형 품종은 주근 및 측근의 생장이 0.2%구에서 양호하였으나, 통일형 품종에서는 대부분의 품종이 주근의 신장은 Casamino acid 무처리구에서 측근의 생장은 0.2%구에서 매우 양호하였다. 5. 분리근의 생장은 배지의 pH, 당농도 및 Casamino acid 농도 등을 달리한 경우에 있어서도 통일형 품종이 일본형 품종보다 양호하였다. 6. 통일형 품종의 교배친중 인도별 품종과 일본형 품종에 있어서 전자는 후자보다 분리근의 생장이 양호하였을 뿐만 아니라 Casamino acid반응이 통일형 품종과 거의 같은 경향인 것으로 나타났다.

• 한국유기농업학회지
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• 제12권4호
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• pp.437-450
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• 2004

This study was conducted with two objectives ; one was to select the suitable soybean cultivars for cultivation in paddy field and the other was to establish the environment-friendly rotational cropping system of soybean instead of rice in paddy field. In order to evaluate growth adaptation and yields, Eve soybean cultivars were cultivated in Yeoncheon, Keonggi province, with two cultivation methods such as level row and high ridge. Growth of the top plants, such as stem length, number of branches, diameter of stem, were higher in high ridge than in level row, however, the differences among the cultivars were bigger than those between the cultivation methods. Dry weight of top plant was significantly different among the cultivars during whole growth stages, however, it was higher in level row than in high ridge at V5 stage while it became higher in high ridge as growth progressed. Roots were more developed in high ridge than in level row during whole growth stages. T/R ratio in level row was higher than that in high ridge. During whole growth stages, significant differences were observed among the cultivars in growth and yields in each cultivation method and yields of Eunhakong was the highest. In results, number of nodules and T/R ratio at V5 stage, number of pods at R2 stage, and number of seeds and T/R ratio at R5 stage had highly correlated with yields, respectively.

• 한국산림과학회지
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• 제108권4호
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• pp.522-530
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• 2019

Two edible plants, Allium victorialis var. platyphyllum and Ligularia stenocephala, and one medicinal plant, Panax ginseng, were cultivated in the understory of an artificial Larix kaempferi plantation for ten years. Growth characteristics (number of leaves and flower stalks per plant, and leaf length and width), survival rate, and yield (fresh weight of plants) per unit area (1 ㎡) were investigated one year after planting, and six and ten years following cultivation. P. ginseng and L. stenocephala survived at a high percentage for two years after planting. Results showed that P. ginseng had longer and thicker roots when aged; however, a large number of plants died and the yield was low. In contrast, almost half of A. victorialis var. platyphyllum died within two years of planting. The surviving plants grew well for ten years and the yield was increased. The leaf length and width of L. stenocephala increased; however, the survival rate and the number of leaves per plant decreased as the period of cultivation was extended. In contrast, A. victorialis var. platyphyllum survived at a lower rate (50%) than the two other crops (98% for L. stenocephala and 83% for P. ginseng) during the early cultivation period, with little change in the survival rate over an extended time; however, the yields increased. This species showed an increase in the number of flower stalks and leaves, and as a result, the larger leaves increased the yield. We evaluated the understory cultivation of three crops in a L. kaempferi plantation under natural conditions, with no irrigation or fertilization, and Allium victorialis var. platyphyllum showed the greatest growth potential among the three tested crops.

• Plant Biotechnology Reports
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• 제3권1호
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• pp.67-74
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• 2009

An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA ($0.5-8{\mu}M$) or Kn ($0.5-8{\mu}M$) alone or in combination with NAA ($0.5-1.5{\mu}M$). Maximum multiple shoot induction was observed on MS medium supplemented with $4{\mu}M$ BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of $1{\mu}M$ NAA along with $4{\mu}M$ BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA ($5{\mu}M$) and 2,4-D ($2{\mu}M$). The callus was subcultured on MS medium supplemented with BA ($2-15{\mu}M$) or Kn ($2-15{\mu}M$) alone or in combination with NAA ($0.5-2{\mu}M$) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with $10{\mu}M$ BA and $1{\mu}M$ NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on half-strength MS medium supplemented with NAA ($1-7{\mu}M$) or IBA ($1-7{\mu}M$). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success. This standardized protocol will help to conserve this rare medicinal plant.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.37-37
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• 2021

Calystegia soldanella L. is a perennial herbaceous halophyte belonging to the convolvulaceae family, which mainly grows in coastal sand dunes in Korea. Shoots and rhizomes are edible, and roots called 'Hyoseon Chogeun' are known to have medicinal effects such as antipyretic, sterilization, and diuretic. In addition, physiological activities of antioxidant, anti-inflammatory, antiviral, antifungal and PTP-1B (protein tyrosine phosphate-1B) inhibition have been reported. In this study, in vitro induction cell lines of C. soldanella L. collected from the coastal sand dunes in Jeju island was redifferentiated into adventitious roots that can be used as medicinal resources. Also the biomass of mass-proliferated adventitious roots using a bioreactor were evaluated. Plants of C. soldanella L. were collected from the crevice of the seashore in the coastal area of Taeheung 2-ri, Namwon-eup, Seogwipo-si. Then, it was separated into leaves, stems, rhizomes, and roots, and surface sterilized with 70% ethyl alcohol and 2% NaOCl (sodium hypochlorite). After washing with sterilized water, each organ section was cultured in Hormone-free MS medium (Murashige & Skoog Medium). As a result, the induction response rates were evaluated at 85% and 55%, respectively, in terms of callus formation and shoot generation in the rhizome segment. In the case of the adventitious roots morphological characteristics induced by single-use treatment of auxin-based plant growth regulators IBA and NAA from redifferentiated shoots were compared. Most efficient adventitious root culture method as a rooting rate, number, length, and biomass proliferation in the bioreactor system was confirmed when treated by culturing in MS salts, Sucrose 30 g·L^-1, and IBA 1mg·L^-1 for 4 weeks. In this study, the medium composition and culture period were confirmed using a bioreactor system to mass-proliferate adventitious roots derived from C. soldanella L. in Jeju island. Also this adventitious root line developed a new medicinal material could increase value of the bio-industry ingredient through quantitative and qualitative screening of phyto-bioactive compounds.

• 한국작물학회지
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• 제36권3호
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• pp.254-258
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• 1991

참 당귀를 시비와 무비 재배조건에서 근두경크기에 따른 생육 및 수량을 검토하여 참당귀재배체계확립을 위한 기초자료로 이용하고자 시험한 결과를 요약하면 다음과 같다. 1. 어느 근두경크기구에서나 초장, 두부경 및 경과과 엽의 생중량은 무비구보다 시비구에서 수치가 높았으나 출현율은 차이가 거의 없었다. 2. 초장과 경\ulcorner엽의 생중량은 7.2mm구에서, 두부경은 5.4mm구를 경계로 하여 그보다 작거나 크면 감소하는 경향이었다. 3. 추태율, 개화율 및 절수는 무비구보다 시비구에서 높았고 근두경이 커짐에 따라 증가하였으나 화총수는 7.2mm구를 경계로 하여 그보다 작거나 크면 감소하는 경향을 나타냈다 4. 근장, 지근수 및 생근중도 역시 시비구에서 높았으며 5.4mm구를 경계로 하여 그보다 작거나 커지면 감사하는 경향을 보였다. 5. l0a당 생근중 및 건근중은 시비의 3.1mm구와 무비의 5.4mm구에서 각각 가장 높게 나타났으나 시비의 3.1mm구가 무비의 5.4mm구보다높은 수치를 보였고, 7.2와 9.3mm구에서는 모두 목질화되었다. 6. 시비와 무비 재배조건하에서 근두경의 크기에 따른 유용형질들의 분산분석에서도 높은 유의차를 보였고 이들의 상호작용에도 모든 형질에서 유의성이 인정되었다.