• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1585-1587
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• 2008

Fibroblast is constantly subjected to mechanical loads in connective tissues where mechanical signals are converted to intercellular biochemical events. The aim of this study is to understand the effects of tensile stress on the neurotrophin (NT) and transforming growth factor (TGF) expression of fibroblast in vitro. Nerve growth factor (NGF) stimulates fibroblast migration, and TGF is related to tissue repair. In this study, at the uniaxial stretch of 10% strain and frequency of 0.5 Hz, different resting times of 0, 20, and 60 min are placed in between 10 min stimulations periods. Results show increase in NGF mRNA levels and a substantial decrease in NT3 mRNA after 1 hr of stimulation, indicating that the tensile stress may regulate NGF and NT3, key factors for the neurocosmetic applications. The mRNA level for TGF-${\alpha}$ and TGF-${\beta}2$ had increased up to two-folds after 1 hr of stimulation, showing that the tensile stress may control TGF, an important part of wound healing.

• Journal of Animal Science and Technology
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• 제63권3호
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• pp.662-665
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• 2021

Streptococcus suis is a major pig pathogen causing severe economic losses to the swine industry. This study aimed to analyze the genome of S. suis strain INT-01 isolated from a domestic pig in Korea. We found that the genome of strain INT-01 contains 2,092,054 bp, with a guanine (G) + cytosine (C) content of 41.3%, and the capsular polysaccharide synthesis locus of this strain is almost identical to that of serotype 3 S. suis strain 4961 isolated from China, suggesting that these isolates can be classified as serotype 3. Genomic analyses revealed that strain INT-01 is an extracellular protein factor (epf)^-/ muraminidase-released protein (mrp)⁺/ suilysin (sly)^- S. suis, which is the most prevalent genotype in Korea, and several virulence-related genes associated with the pathogenicity of S. suis were also detected. The genomic information of strain INT-01 may provide important insights into the development of control strategies against S. suis infections in Korea.

• Reproductive and Developmental Biology
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• 제31권2호
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• pp.103-108
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• 2007

This study was carried out to investigate the effects of cryoprotectants, warming solution and removal of lipid on open pulled straw vitrification (OPS) method of porcine embryos produced by nuclear transfer (NT) of fetal fibroblasts. All solutions used during vitrification were prepared with holding medium consisting of 25 mM Hepes buffered TCM199 medium containing 20% fetal bovine serum (FBS) at $38.5^{\circ}C$. The blastocysts derived from NT with or without lipid were vitrified in each medium of different concentrations of dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Also, blastocysts after cryopreservation were warmed into different concentrations of sucrose in warming solution. The optimal concentrations of cryoprotectants in vitrification solution were 10% DMSO + 10% EG in vitrification solution 1 (VS1) and 20% DMSO + 20% EG in vitrification solution 2 (VS2). The optimal concentrations of sucrose were 0.3 M sucrose in warming solution 1 (WS1) and 0.15 M sucrose in warming solution 2 (WS2). lipid removal from oocytes before NT enhanced the viability of NT embryos after vitrification. Our results show that use of the OPS method in conjunction with lipid removal provides effective cryopreservation of porcine nuclear transfer embryos.

• Molecules and Cells
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• 제27권6호
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• pp.657-665
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• 2009

The ovomucoid pre-mRNA has been folded into mini-hairpins adaptable for the RNA recognition motif (RRM) protein binding. The number of mini-hairpins were 372 for pre-mRNA and 83-86 for mature mRNA. The spatial arrangements are, in average, 16 nucleotides per mini-hairpin which includes 7 nt in the stem, 5.6 nt in the loop and 3.7 nt in the inter-hairpin spacer. The constitutive splicing system of ovomucoid-pre-mRNA is characterized by preferred order of intron removal of 5/6 > 7/4 > 2/1 > 3. The 5' splice sites (5'SS), branch point sequences (BPS) and 3' splice sites (3'SS) were identified and free energies involved have been estimated in 7 splice sites. Thermodynamic barriers for splice sites from the least (|lowest| -Kcal) were 5, 4, 7, 6, 2, 1, and 3; i.e., -18.7 Kcal, -20.2 Kcal, -21.0 Kcal, -24.0 Kcal, - 25.4 Kcal, -26.4 Kcal and -28.2 Kcal respectively. These are parallel to the kinetic data of splicing order reported in the literature. As a result, the preferred order of intron removals can be described by a consideration of free energy changes involved in the spliceosomal assembly pathway. This finding is consistent with the validity of hnRNP formation mechanisms in previous reports.

• Advances in nano research
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• 제10권3호
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• pp.211-219
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• 2021

The evaluation of the corrosion resistance of titanium with a TiO2 nanotubes top layer was carried out (TiO2 NT). These nanostructures were evolved into anatase nanoparticles without heat treatment in an aqueous medium, which is a novel phenomenon. This work analyzes the layer between the nanotube bottom and the substrate, which is thin and still susceptible to corrosion. The bottom of TiO2 nanotubes having Fluor resulting from the synthesis process changed between amorphous to crystalline anatase with a crystallite size of about 4 nm, which influenced the corrosion rates. Four kinds of samples were evaluated. A) NT by Ti anodizing; B) NTSB for Ti plates, either modifying its surface or anodizing the modified surface; C) NT-480 for anodized Ti and heat-treated (480℃) for reaching the anatase phase; D) NTSB-480 for Ti plates, first, modifying its surface using sandblast, after that, anodizing the modified surface, and finally, heat-treated to 480℃ to compare with samples having induced crystallization and passivation. Four electrochemical techniques were used to evaluate the corrosion rates. The surfaces having TiO2 nanotubes with a sandblast pre-treatment had the highest resistance to corrosion.

• 한국초지조사료학회지
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• 제14권3호
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• pp.195-200
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• 1994

This experiment was undertaken to study the relationship between dry weight of plant(DW) and yield components. varietal differences in seasonal production at each of three cutting. and to classiQ them into some patterns of seasonal production in tall fescue varieties. The varieties examined were Barvetia. Fuego, Demeter. Safe, Barcel. Forager, Johnstone. Enforcer and Stef. The results obtained were as follows : 1. The Fuego and Barcel with high number of tiller per plant(NT) which had more dry wieght of plant(DW) than those of varieties with low number of tillers per plant(NT) at each cutting. 2. The dry weight of plant(DW) in 1st cutting indicated positive significant correlation with the number of tillers per plant(NT) and dry weight of a tiller(WT). But there was only positive correlation between the number of tillers per plant(NT) and dry weight of plant(DW) in 2nd and 3rd cutting. 3. Mean relative yield of each cutting were 3 1 %. 29.7% and 39.3% in 1st. 2nd and 3rd cutting, respectively. 4. Tall fescue varieties could be classified into 3 different seasonal growth patterns according to their mode of relative yield at each cutting. Barvetia, Fuego, Johnstone. Enforcer and Stef belong to the group of ABA with high relative yield in 1st and 3rd cutting. Demeter, Safe and Forager belong to the group of CBA with relative yield increased linearly up to 3rd cutting. And, Barcel belong to the group of BAB with high relative yield in 2nd cutting.

• 전자통신동향분석
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• 제24권6호
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• pp.11-21
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• 2009

에너지를 절약하고 제품을 축소하기 위하여 전력공급 장치나 전력변환 장치에 사용되는 전력반도체는 전력용 파워스위칭 소자와 제어 IC로 구성되어 전력을 시스템에 맞게 배분하는 제어와 변환기능을 가진 반도체로 단순히 전력을 조절하고 전달하는 역할에서 에너지효율 제고 및 시스템 안정성과 신뢰성을 좌우하는 역할로 확장되어 가고 있고, 교토의정서 등의 지구 온난화 방지노력과 글로벌 환경규제의 확대로 친환경 절전형 부품/시스템 개발이 절실히 요구되는 실정이다. 이에 따라, 본 고에서는 스마트환경, 그린에너지, 예방진단 등 미래 인간생활 대응을 통해 신기술 및 신시장을 창출하는 신성장 동력 분야인 저전력, 고효율, 저발열, 저소음 등 환경 친화적으로 동작하여 에너지 효율 및 $CO_2$ 배출에 직접적인 영향을 미치는 친환경 절전형 전력반도체 기술 동향에 대해 논의하고자 한다.

• 한국고분자학회지:고분자과학과기술
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• 제15권2호
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• pp.158-172
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• 2004

인류는 급격한 산업화가 몰고 온 부작용으로 물, 공기, 토양 오염을 통한 생태계 파괴라는 엄청난 환경재앙을 맞고 있다. 물에는 각종 화학물질이 흘러들고, 공기에는 유해한 기체가 과도하게 함유되며, 토양에는 중금속이 축적됨으로 인해 인간을 비롯한 모든 동식물에게 있어 생명의 원천이며 삶의 터전인 지구의 생태계 균형이 파괴되고 있다. 최근 이런 환경문제를 해결하기 위해 환경기술 (environmental technology, ET)과 다양한 기술을 융합해 상승효과를 얻고자 하는 융합기술 (fusion technology, FT)에 대한 시도와 연구가 활발히 진행되고 있다. 그중에서도 나노기술 (nanotechnology, NT)을 접목한 나노(NT)/환경 (ET) 융합형 기술이 실적용에 따른 획기적인 환경 개선효과와 가능성으로 인해 최근 가장 큰 주목을 받고 있다.(중략)

• 한국산업융합학회 논문집
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• 제7권2호
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• pp.173-179
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• 2004

In this paper, we developed a Windows NT version off-line programming system which can simulate a track vehicle model in 3D graphics space. The track vehicle was adopted as an objective model. The interface between users and the off-line program system in the Windows NT's graphic user interface environment was also studied. The developing language is Microsoft Visual C++. Graphic libraries, OpenGL, by Silicon Graphics, Inc. were utilized for 3D Graphics.

• Korean Journal of Radiology
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• 제19권6호
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• pp.1099-1109
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• 2018

Objective: In a proof of concept study, we compared no-touch radiofrequency ablation (NtRFA) in bipolar mode with conventional direct tumor puncture (DTP) in terms of local tumor control (LTC), peritoneal seeding, and tumorigenic factors, in the rabbit VX2 subcapsular hepatic tumor model. Materials and Methods: Sixty-two rabbits with VX2 subcapsular hepatic tumors were divided into three groups according to the procedure: DTP-RFA (n = 25); NtRFA (n = 25); and control (n = 12). Each of the three groups was subdivided into two sets for pathologic analysis (n = 24) or computed tomography (CT) follow-up for 6 weeks after RFA (n = 38). Ultrasonography-guided DTP-RFA and NtRFA were performed nine days after tumor implantation. LTC was defined by either achievement of complete tumor necrosis on histopathology or absence of local tumor progression on follow-up CT and autopsy. Development of peritoneal seeding was also compared among the groups. Serum hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) were measured via ELISA (Elabscience Biotechnology Co.) after RFA for tumorigenic factor evaluation. Results: Regarding LTC, there was a trend in NtRFA (80%, 20/25) toward better ablation than in DTP-RFA (56%, 14/25) (p = 0.069). Complete tumor necrosis was achieved in 54.5% of DTP-RFA (6/11) and 90.9% of NtRFA (10/11). Peritoneal seeding was significantly more common in DTP-RFA (71.4%, 10/14) than in NtRFA (21.4%, 3/14) (p = 0.021) or control (0%). Elevations of HGF, VEGF or IL-6 were not detected in any group. Conclusion: No-touch radiofrequency ablation led to lower rates of peritoneal seeding and showed a tendency toward better LTC than DTP-RFA.