• Research in Plant Disease
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• v.29 no.3
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• pp.286-294
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• 2023

Biological and molecular characterization of a Korean isolate of Orthotospovirus chrysanthinecrocaulis (formerly known as chrysanthemum stem necrosis virus, CSNV) isolated from Chrysanthemum morifolium was determined using host range and sequence analysis in this study. Twenty-three species of indicator plants inoculated mechanically CSNV-Kr was investigated for determination of host range. CSNV-Kr induced various local and systemic symptoms in the inoculated plant species. CSNV-Kr could not infect three plant species and induced symptomless in systemic leaves in Nicotiana tabacum cultivars, though the plant samples reacted positively with the antiserum to CSNV by double-antibody sandwich-enzyme-linked immunosorbent assay. The complete genome sequence of CSNV-Kr was determined. The L RNA of CSNV-Kr consists of 8,959 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase. The M RNA of CSNV-Kr consists of 4,835 nt and encodes the movement protein (NSm) and the glycoprotein precursor (Gn/Gc protein). The S RNA of CNSV-Kr consists of 2,836 nt and encodes NSs protein and N protein. The Gn/Gc and N sequence of CSNV-Kr were compared with those of previously published CSNV isolates originating from different countries at nucleotide and amino acid levels. The Gn/GC sequence of CSNV-Kr shared 98.8-99.5% identity with CSNV isolated from other countries and the N sequence of CSNV-Kr shared 98.8-99.6% identity. No particular region of variability could be found in either grouping of viruses. All of the CSNV isolates did not show any relationship according to geographical origins and isolation hosts, suggesting no distinct segregation of the CSNV isolates.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.3A
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• pp.349-356
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• 2008

Recently FRP (Fiber Reinforcement Polymer) is widely used for the strengthening of damaged RC beams. Although many tests were carried out to verify flexural capacity of RC beams strengthened with FRP sheet or plate, the behavior of strengthened RC beams has not yet clearly verified. To investigate the strengthening efficiency of the Near Surface Mounted Reinforcement (NSMR) technique experimentally and analytically, a total of 7 specimens have been tested. The experimental results revealed that specimens strengthened with NSMR improved the flexural capacity of RC beams. Also, while the NSMR specimens utilized CFRP reinforcement efficiently compared to the EBR (Externally Bonded Reinforcement) specimen, the NSMR specimens still have debonding failure between epoxy and concrete interface. This study has proposed the model to predict failure modes and failure loads. Good agreement was obtained between the predicted and the experimental results.

• Ocean and Polar Research
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• v.35 no.1
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• pp.1-14
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• 2013

Shoreline data of the barrier islands in Nakdong River Estuary for the last three decades were assembled using six sets of aerial photographs and seven sets of satellite images. Canny Algorithm was applied to untreated data in order to obtain a wet-dry boundary as a proxy shoreline. Digital Shoreline Analysis System (DSAS 4.0) was used to estimate the rate of shoreline changes in terms of five statistical variables; SCE (Shoreline Change Envelope), NSM (Net Shoreline Movement), EPR(End Point Rate), LRR (Linear Regression Rate), and LMS (Least Median of Squares). The shoreline in Jinwoodo varied differently from one place to another during the last three decades; the west tail has advanced (i.e., seaward or southward), the west part has regressed, the south part has advanced, and the east part has regressed. After the 2000s, the rate of shoreline changes (-2.5~6.7 m/yr) increased and the east advanced. The shoreline in Shinjado shows a counterclockwise movement; the west part has advanced, but the east part has retreated. Since Shinjado was built in its present form, the west part became stable, but the east part has regressed faster. The rate of shoreline changes (-16.0~12.0 m/yr) in Shinjado is greater than that of Jinwoodo. The shoreline in Doyodeung has advanced at a rate of 31.5 m/yr. Since Doyodeung was built in its present form, the south part has regressed at the rate of -18.2 m/yr, but the east and west parts have advanced at the rate of 13.5~14.3 m/yr. Based on Digital Shoreline Analysis, shoreline changes in the barrier islands in the Nakdong River Estuary have varied both temporally and spatially, although the exact reason for the shoreline changes requires more investigation.

• Microbiology and Biotechnology Letters
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• v.34 no.3
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• pp.257-264
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• 2006

An aerobic submerged membrane bioreactor (MBR) treating ammonium wastewater was studied in respect of nitrification characteristics and distribution of nitrification bacteria over a period of 350 days. MBR was fed with ammonium concentration of 500-1000 mg $NH_4-N/L$ at a nitrogen load of $1-2kg\;N/m^3{\cdot}d$. Overall ammonium oxidation rate increased with dissolved oxygen (DO) concentration, temperature, and sludge retention time (SRT). Under a higher concentration of free ammonia ($NH_3-N$) due to the decrease of ammonium oxidation rate, the nitrite ratio ($NO_2-N/NO_x-N$) in the effluent increased. The sudden collapse of nitrification efficiency accompanied by sludge foaming and the increase of sludge volume index (SVI) was observed unexpectedly during the operation. At the later stage of operation, additional carbon source was fed to the MBR and resulted in twice higher value of SVI and the decrease of ammonium oxidation rate. In fluorescence in situ hybridization (FISH) analysis, genus Nitrosomonas which is specifically hybridized with probe NSM156 was initially the dominant ammonia oxidizing bacteria and the amount of Nitrosospira gradually increased. Nitrospira was the dominant nitrite oxidizing bacteria during whole operational period. Significant amount of Nitrobacter was also detected which might due to the high concentration of nitrite maintained in the reactor.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.4
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• pp.126-132
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• 2018

As means to increase the use of concrete with recycled coarse aggregate (RCA), this study aims to evaluate the applicability for flexural strengthening of reinforced concrete beam with high-strength concretes and RCA on which FRP plates, used for repair and strengthening of old and low-durability reinforced concrete structures, is applied. In order to increase the adhesive force of epoxy and FRP plate, FRP plate was installed according to Near-Surface-Mounted (NSM) method. 12 specimens were manufactured using substitution rate of RCA (30%), concrete strengths (40MPa, 60MPa), diameters of deformed bar (D10, D13), and types of FRP plate (AFRP, CFRP) as variables to analyse flexural performance according to FRP plate and substitution rate of recycled aggregate. As a result, in all specimens, specimens strengthened by FRP plate showed a maximum of 17% increase in performance compared to specimens without FRP plate and strengthening performance of CFRP was found to be higher than AFRP. When modulus of rupture was used, the value of cracking moment was similar to that of the reference equation. As bending moment of some specimens strengthened by FRP plate failed to satisfy the criteria of KCI 2012 and ACI 440-2R, additional experiment is deemed as necessary.

• Journal of Energy Engineering
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• v.16 no.4
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• pp.155-163
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• 2007

To insure nuclear reactor safety, the reactivity of control rods should be calculated by measuring the criticality of reactor core and it is regularly performed during the annual physics test period. Also, the core criticality should be monitored during the start-up operation to avoid reactivity induced accidents. Many research works on control rod reactivity measurement and subcriticality measurement have been accomplished throughout the world for decades and recently a new method named "Modified Neutron Source Multiplication Method (MNSM)" was proposed in Japan which is known to be improved overcoming limitations of traditional Neutron Source Multiplication Method (NSM). In this study, MNSM was tested in calculation of subcriticalities and in evaluation of application validity using the educational reactor in Kyung Hee University, AGN-201. For this study, a revised nuclear data library and a neutron transport code system TRANSX - PARTISN were established. Correction factors for various control rod positions were produced using the k-effective values and the corresponding flux distributions and adjoint flux distributions. Experimental values of the core criticality were obtained using the neutron count rates of the BF3 proportional counters. The results showed that the expected reactivity worth of control rods by MNSM agreed well with the theoretical values and the correction factors contributed much for this purpose.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1751-1760
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• 2015

Mucin2 (MUC2), an important regulatory factor in the immune system, plays an important role in the host defense system against bacterial translocation. Probiotics known to regulate MUC2 gene expression have been widely studied, but the interactions among probiotic, pathogens, and mucin gene are still not fully understood. The aim of this study was to investigate the role of MUC2 in blocking effects of probiotics on meningitic E. coli-induced pathogenicities. In this study, live combined probiotic tablets containing living Bifidobacterium, Lactobacillus bulgaricus, and Streptococcus thermophilus were used. MUC2 expression was knocked down in Caco-2 cells by RNA interference. 5-Aza-2'-deoxycytidine (5-Aza-CdR), which enhances mucin-promoted probiotic effects through inducing production of Sadenosyl-L-methionine (SAMe), was used to up-regulate MUC2 expression in Caco-2 cells. The adhesion to and invasion of meningitic E. coli were detected by competition assays. Our studies showed that probiotic agents could block E. coli-caused intestinal colonization, bacteremia, and meningitis in a neonatal sepsis and meningitis rat model. MUC2 gene expression in the neonatal rats given probiotic agents was obviously higher than that of the infected and uninfected control groups without probiotic treatment. The prohibitive effects of probiotic agents on MUC2-knockdown Caco-2 cells infected with E44 were significantly reduced compared with nontransfected Caco-2 cells. Moreover, the results also showed that 5-Aza-CdR, a drug enhancing the production of SAMe that is a protective agent of probiotics, was able to significantly suppress adhesion and invasion of E44 to Caco-2 cells by upregulation of MUC2 expression. Taken together, our data suggest that probiotic agents can efficiently block meningitic E. coli-induced pathogenicities in a manner dependent on MUC2.