• Title/Summary/Keyword: NS5A

Search Result 712, Processing Time 0.028 seconds

The Study on the Quality Characteristics of Cake Prepared with Fat Substitute (대체지방으로 제조한 케이크의 품질 특성에 관한 연구)

  • Woo, Na-Ri-Yah;Ahn, Myung-Soo
    • Journal of the Korean Society of Food Culture
    • /
    • v.19 no.5
    • /
    • pp.506-515
    • /
    • 2004
  • This study was carried on the quality characteristics of cake prepared with Co(com oil) containing MCT(medium chain triglycerides), which is used as a fat substitute. In tile cake prepared with MCT-CO containing $10{\sim}30%$ MCT, the appearance and the degree of gelatinization were similar to those of cake prepared with NS-CO(non-substituted corn ell) Cake batter prepared with MCT-CO showed higher specific gravity and consistency than those of cake prepared with NS-CO and increased by increasing MCT contents. The baking loss of cake prepared with MCT-CO was more than that of cake prepared with NS-CO and increased by increasing MCT contents as well. The rheological properties of cake represented significant different by MCT substitution ratio(p<0.05). Gumminess, hardness and chewiness of cake prepared with MCT-CO containing $20{\sim}30%$ MCT were about 2 times higher than those of cake prepared with NS-CO, while springiness and cohesiveness showed similar pattern between the cake prepared with MCT-CO or NS-CO. In sensory evaluation, appearance. color and flavor of cake prepared with CO substituted by MCT up to 100% were better assessed and the taste was better in range of 30% MCT-CO but the texture was less accepted than that of cake prepared with NS-CO.

Sequence Analysis of NS4 Region of HCV Isolated from Korean Patient

  • Paik, Sang-Hoon;Lee, Young-Ik;Kim, Won-Bae;Yang, Jai-Myung
    • Journal of Microbiology
    • /
    • v.33 no.3
    • /
    • pp.260-266
    • /
    • 1995
  • Hepatitis C virus (HCV) has been considered as a mojor causative agent of post-transfusion related non-A, non-B hepatitis. In this study, the cDNA sequence of NS4 region of HCV (HCV-S) obtained from a Korean patient's plasms was determined. Comparative nucleotide sequence analysis between to type II. 67.2% homology to type III, and 66.4% homology to type IV. The putative amino acid sequence homologies to types I, II, III, and IV were 82.8-84.7%, 92.5-95.1%. 72.5, and 71.1%, respectively. This data strongly suggests that HCV-S should be classified as type II. Significant similarities of hydrophobicity profiles and putative transmembranous domains were found in HCV-S and four major prototypes, indicating that the protein structure is similar in spite of the heterogeneities of intertype homologies at the level of the psrimary nucleotide and amino acid sequences.

  • PDF

Quantification of Vibrio parahaemolyticus Using a Most Probable Number-Polymerase Chain Reaction Assay Targeting the H-NS gene (MPN 및 H-NS 유전자를 표적으로 하는 PCR assay를 병용한 장염비브리오(Vibrio parahaemolyticus)의 정량)

  • Kim, Tae-Ok;Park, Kwon-Sam
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.47 no.5
    • /
    • pp.556-561
    • /
    • 2014
  • We applied a combination of most probable number-polymerase chain reaction (MPN-PCR) methods using a PCR procedure targeting the H-NS (VP1133) gene to detect Vibrio parahaemolyticus presence and density in seawater as well as within short-necked clam Ruditapes philippinarum tissues collected from Gomso Bay, Korea. In 30 seawater samples, V. parahaemolyticus levels ranged from less than 1.8 to $1.1{\times}10^3MPN/100mL$, and samples from August showed higher than those from other months. Furthermore, the levels of V. parahaemolyticus in six short-necked clam samples ranged from $7.8{\times}10^2$ to $2.1{\times}10^3MPN/100g$, approximately 2.5 times higher than in seawater samples from the corresponding month. Our results provide data on V. parahaemolyticus contamination in seawater and short-necked clam tissues, and help to improve quantitative methods of assessing V. parahaemolytcius levels.

A Design of 10bit current output Type Digital-to-Analog converter with self-Calibration Techique for high Resolution (고해상도를 위한 DAC 오차 보정법을 가진 10-비트 전류 출력형 디지털-아날로그 변환기 설계)

  • Song, Jung-Gue;Shin, Gun-Soon
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.12 no.4
    • /
    • pp.691-698
    • /
    • 2008
  • This paper describes a 3.3V 10 bit CMOS digital-to-analog converter with a divided architecture of a 7 MSB and a 3 LSB, which uses an optimal Thermal-to-Binary Decoding method with monotonicity, glitch energy. The output stage utilizes here implements a return-to-zero circuit to obtain the dynamic performance. Most of D/A converters in decoding circuit is complicated, occupies a large chip area. For these problems, this paper describes a D/A converter using an optimal Thermal-to-Binary Decoding method. the designed D/A converter using the CMOS n-well $0.35{\mu}m$ process0. The experimental data shows that the rise/fall time, settling time, and INL/DNL are 1.90ns/2.0ns, 12.79ns, and a less than ${\pm}2.5/{\pm}0.7\;LSB$, respectively. The power dissipation of the D/A converter with a single power supply of 3.3V is about 250mW.

Monolithic and Resolution with design of 10bit Current output Type Digital-to-Analog Converter (개선된 선형성과 해상도를 가진 10비트 전류 출력형 디지털-아날로그 변환기의 설계)

  • Song, Jun-Gue;Shin, Gun-Soon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2007.10a
    • /
    • pp.187-191
    • /
    • 2007
  • This paper describes a 3.3V 10 bit CMOS digital-to-analog converter with a divided architecture of a 7 MSB and a 3 LSB, which uses an optimal Thermal-to-Binary Decoding method with monotonicity, glitch energy. The output stage utilizes here implements a return-to-zero circuit to obtain the dynamic performance. Most of D/A converters in decoding circuit is complicated, occupies a large chip area. For these problems, this paper describes a D/A converter using an optimal Thermal-to-Binary Decoding method. the designed D/A converter using the CMOS n-well $0.35{\mu}m$ process0. The experimental data shows that the rise/fall time, settling time, and INL/DNL are 1.90ns/2.0ns, 12.79ns, and a less than ${\pm}2.5/{\pm}0.7$ LSB, respectively. The power dissipation of the D/A converter with a single power supply of 3.3V is about 250mW.

  • PDF

Evaluation of Inhibitory Effects of Thiobarbituric Acid Derivatives Targeting HCV NS5B Polymerase

  • Lee, Jong-Ho;Lee, Sang-Yoon;Park, Mi-Young;Ha, Hyun-Joon;Myung, Hee-Joon
    • Journal of Microbiology and Biotechnology
    • /
    • v.20 no.3
    • /
    • pp.510-512
    • /
    • 2010
  • A series of thiobarbituric acid derivatives were constructed and evaluated for inhibitory activity on hepatitis C virus NS5B polymerase. In biochemical assays using purified viral polymerase and RNA template, the $IC_{50}$ value was improved to 0.41 ${\mu}M$ from the original compound's 1.7 ${\mu}M$ value. In HCV sub genomic replicon assay, the $EC_{50}$ value was improved to 3.7 ${\mu}M$ from the original compound's 12.3 ${\mu}M$ value. $CC_{50}$ was higher than 77 ${\mu}M$ for all compounds tested, suggesting that they are useful candidates for anti-HCV therapy.

Effects of Nitrogen Fertilization on Physiological Characteristics and Growth of Populus sibirica Seedlings in a Semi-arid Area, Mongolia (몽골 반건조지에서의 질소 시비가 Populus sibirica 묘목의 생리 및 생장 특성에 미치는 영향)

  • Chang, Hanna;Han, Seung Hyun;Kang, Hoduck;Akhmadi, Khaulenbek;Son, Yowhan
    • Journal of Korean Society of Forest Science
    • /
    • v.106 no.1
    • /
    • pp.19-25
    • /
    • 2017
  • This study was conducted to investigate the effects of nitrogen fertilization on physiological characteristics and growth of Populus sibirica seedlings in a semi-arid area, Mongolia. 2-year-old P. sibirica seedlings were planted in May, 2015 with applications of urea 5 g (N1), 15 g (N2), 30 g (N3) and ammonium sulfate 33 g (NS; same nitrogen amount with N2) to each seedling. Chlorophyll contents were significantly different among treatments in August, but not in June and July. The lower chlorophyll contents in August than those in June and July might be related to leaf senescence. In June and July, net photosynthetic rate was higher in NS and N2 than in the control. Unlike the tendency of photosynthesis, transpiration rate was highest in N2, but lower in NS than in any other nitrogen treatments. Relative growth rate of root collar diameter was significantly higher only in NS than in the control and it of height did not differ among treatments. Leaf area in nitrogen treatments was not significantly different from that in the control. Ammonium sulfate seemed to be more suitable fertilizer than urea for the early growth of P. sibirica seedlings in the study site. However, as the effects of urea and ammonium sulfate on soils and seedlings were different, further studies would be necessary to determine the optimal amount of ammonium sulfate.

A Study on the Switching and Retention Characteristics of PLT(5) Thin Films (PLT(5) 박막의 Switching 및 Retention 특성에 관한 연구)

  • Choi Joon Young;Chang Dong Hoon;Kang Seong Jun;Yoon Yung Sup
    • Proceedings of the IEEK Conference
    • /
    • 2004.06b
    • /
    • pp.367-370
    • /
    • 2004
  • We fabricated PLT(5) thin film on $Pt/TiO_x/SiO_2/Si$ substrate by using sol-gel method and investigated leakage current, switching and retention properties. The leakage current density of PLT(5) thin film was $3.56{\times}10^{-7}A/cm^2$ at 4V. In the examination of switching properties, pulse voltage and load resistance were $2V{\~}5V$ and $50{\Omega}{\~}3.3k{\Omega}$, respectively. Switching time had a tendency to decrease from 520ns to 140ns with the increase of pulse voltage, and also the time was increased from 140ns to $13.7{\mu}s$ with the increase of load resistance. The activation energy obtained from the relation of applied pulse voltage and switching time was about 143kV/cm. The error of switched charge density between hysteresis loop and experiment of polarization switching was about $10\%$. Also, polarization in retention was decreased as much as about $8\%$ after $10^5$s.

  • PDF

Hepatoprotective Effects of Fermented Smilax china Leaf Extract on Carbon Tetrachloride-induced Liver Injury in Mice (발효 청미래덩굴잎 추출물이 사염화탄소에 의한 마우스의 간 손상 보호 효과)

  • Kim, Mee-Jung
    • Journal of the East Asian Society of Dietary Life
    • /
    • v.24 no.2
    • /
    • pp.166-175
    • /
    • 2014
  • This study was conducted to investigate the hepatoprotective effects of fermented Smilax china leaf ethylacetate extracts by Aspergillus oryzae on carbon tetrachloride-induced liver injury in mice. Experimental mice were divided into four groups (five mice/group) (NC; normal control group, CB; basic diet supplemented before $CCl_4$ treatment group, NS ; basic diet mixed with 0.5% Smilax china leaf ethyl acetate extract supplemented before $CCl_4$ treatment group, FS; basic diet mixed with 0.5% fermented Smilax china leaf ethyl acetate extract supplemented before $CCl_4$ treatment group) fed for 4 weeks each. In the $CCl_4$-treated groups (CB, NS and FS) compared with the NC group, liver weights, activities of alanine aminotransferase, aspartate aminotransferase, xanthine oxidase and aldehyde oxidase, contents of triglycerides, total cholesterol and LDL-cholesterol in serum, and hepatic lipid peroxide levels increased, whereas body weight gain and contents of glutathione and HDL-cholesterol decreased. Furthermore, in the FS groups compared with the NS and CB groups, increased or decreased indicators by $CCl_4$ treatment significantly decreased or increased, respectively. This study suggests that fermented Smilax china L. leaf extracts may regulate xanthine oxidase and aldehyde oxidase inhibitory activities and hepatoprotective effects due to flavonoid aglycone derived from its glycoside in leaves of Smilax china by fermentation of A. oryzae.

Design of High Speed Switching Circuit for Pulsed Power Amplifier (Pulsed Power Amplifier를 위한 고속 스위칭 회로 설계)

  • Yi, Hui-Min;Hong, Sung-Yong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.19 no.2
    • /
    • pp.174-180
    • /
    • 2008
  • The pulsed amplifier which switches the main supply voltage of RF amplifier according to input pulse signal has good efficiency and low noise level between pulses. And it has simple structure because it doesn't need a pulse modulator at input port. The pulsed amplifier using the conventional switching circuit has slow fall time compared to rise time. We proposed the novel switching circuit for improving the fall time of pulsed amplifier The proposed switching circuit is implemented by replacing FET of conventional circuit with BJT. As a result of appling this circuit to RF pulsed amplifier, the rise and fall time are 5.7 ns and 21.9 ns at 27 dBm output power, respectively.