• Korean journal of applied entomology
• /
• v.38 no.2
• /
• pp.145-149
• /
• 1999

To develop the baculovirus expression vector system (BEVS) adopting p10 gene promoter of Spodoptera exigua nucleopolyhedrovirus (SeNPV), we characterized the p10 gene of SeNPV. The nucleotide sequence of 545 bases including the coding region of p10 gene was determined. Compared with the previously reported SeNPV p10 gene (Zuidema et al., 1993), 4 bases were different in the 5' and 3' flanking region but no difference was found in the coding region. The p10 gene was located within Xho I 1.5 Kb, Sph 1 2.4 Kb and Cla I 4.0 Kb fragments by Southern hybridization analysis. Also, the Sph I 2.4 Kb and the Cla I 4.0 Kb fragments were cloned and their restriction enzyme maps were determined.

• BMB Reports
• /
• v.39 no.6
• /
• pp.737-742
• /
• 2006

Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight of 31.0 kDa. Bm60 and its homologues have been identified in 11 completely sequenced lepidopteran NPVs. The transcript of Bm60 was detected by RT-PCR at 18-72 h post-infection (p.i.), while the corresponding protein could be detected at 24-72 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm60. The expression of Bm60 was inhibited in the presence of Ara-c, an inhibitor of viral DNA synthesis. These results together indicated that Bm60 was a late gene. The size of Bm60 product was found to be a 31 kDa in BmNPV-infected BmN cells, consistent with predicted molecular weight. Immuno-fluoresence analysis showed that the Bm60 product was first detected in the cytoplasm at 24 h p.i and also located in nucleus during later infection. In conclusion, the available data suggest that Bm60 is a functional ORF of BmNPV and encodes a 31kDa protein expressed in the later stage of infection cycle.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.1 no.1
• /
• pp.29-33
• /
• 2000

We cloned and characterized a very late expression factor 1 gene, vlf-1, which regulates the level of very late gene transcripts, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 1,140 bp vlf-1 has an open reading frame of 379 amino acid and a MW of 44 kDa. The vlf-1 nucleotide sequence of BmNPV-Kl showed high homology with Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain so far known, and its deduced amino acid residues were identical to those of BmNPV T3. The location of vlf-1 in the BmNPV-Kl genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level were confirmed by Northern hybridization analysis.

• Journal of Agricultural Extension & Community Development
• /
• v.19 no.4
• /
• pp.799-831
• /
• 2012

This study was accomplished to support farmers who want to introduce Automatic Milking System. The methods of analysis is considered on it as investment analysis that NPV, ROV and FROV. As a classical investment analysis technique, NPV showed 142 thousand won on the every senarioes. On the other hands, The Real Option Analysis showed 153,826, 154,937 and 152,858 on the normal, optimistic and pessimistic senarioes respectively. it is considered as a investment analysis technique for strategic decision-making. But, it may have problem to evaluate present value of expected cash flows and expected costs by a single number. To solve those problems, this paper tried to evaluate Fuzzy Real Option Model which were jointed with a real option model and Fuzzy set model. The result of analysis showed, on respective senarioes, 153,515 to 161,489, 154,612 to 162,970, and 152,573 to 159,835 on the interval estimation. Thereby It is a more realistic in many cases.

• Journal of Sericultural and Entomological Science
• /
• v.33 no.1
• /
• pp.21-26
• /
• 1991

Bombyx mori nuclear polyhedrosis virus (BmNPV) was successfully multiplied in the nuclear of BmN4 cells cultured with insect Grace's medium. By electron microscopic observation, the virons had a single nucleocapsid in an envelope. Polyhedral protein synthesis of BmNPV in BmN4 cells was detected at 18 hr p.i. and polyhedral protein was a singlepolypeptide with a M.W of 30 kd. At 48 hr p.i. polyhedra formation was observed by inverted mociroscope and electron microscope. Genome analysis of BmNPV by restriction endonucleases was not revealed the difference between virus produced in vivo and that in vitro.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.20 no.5
• /
• pp.321-326
• /
• 2008

This paper presents and analyzes the effects of on-grid electricity cost, fuel price and initial capital cost of a CHP system, on the optimum DG and AC capacity and NPV, by using the ORNL CHP Capacity Optimizer, which was applied to a library in a university. By considering the current domestic energy cost and initial capital cost, it is shown that the installation and operation of the CHP system is not economical. However, with the current domestic CHP installation cost and fuel price, the NPV achieved by the installation of CHP system is greater when the on-grid electricity price is a factor of ${\times}1.5$ the present value. Regarding the initial capital cost of the CHP system, the reduction of the DG cost is much more economical than that of the AC cost, with respect to NPV. Electricity cost and fuel price have opposite effects on NPV, and NPV is more sensitive to an increase of the electricity cost than an increase of the fuel price.

• Microbiology and Biotechnology Letters
• /
• v.25 no.4
• /
• pp.359-366
• /
• 1997

The usefulness of host range expanded recombinant viruses for economical viral insecticide and expression vector system has been studied. Host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Bombyx mori nuclear polyhedrosis virus (BmNPV) and Autographa californica NPV (AcNPV), and a host range expanded AcNPV recombinant, Ac-BH, constructed by substitution of the 0.6Kb fragment of the BmNPV helicase gene were compared. The restriction enzyme digestion patterns showed that RecS-B6 and RecB-8 had expanded host ranges by genomic recombination and were more similar to genome of AcNPV than that of BmNPV. SDS-PAGE and PCR analysis showed that the polyhedrin gene of RecS-B6 and RecB-8 was derived from BmNPV genomic DNA. The morphology of polyhedra of recombinant viruses showed a slight difference between the two host cells, Sf and BmN cells, indicating that the morphology of polyhedra was influenced by host cells. The bioassay data for insect larvae showed that Ac-BH, compared to wild type viruses, had superior pathogenicity against Bombyx mori larvae but inferior pathogenicity against Spodoptera exigua larvae. Although the pathogenicity was lower than that of wild type viruses in both larvae, RecS-B6 showed the pathogenicity in both larvae. These results suggested that Ac-BH was a less useful economical insecticide than random genomic recombinant virus RecS-B6.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2022.11a
• /
• pp.300-301
• /
• 2022

In order to improve the accessibility to the island through marine leisure ships, it is necessary to evaluate objective business feasibility of the ship's operation. In this study, costs and income were estimated based on the operating expenses and freight standards of similar marine leisure ships in operation, and based on this, the feasibility of the project was empirically analyzed through B/C analysis, NPV, and IRR. In addition, sensitivity analysis was performed for various variables to understand the requirements for securing minimum economic feasibility. As a result of the study, in case of new building ship, the B/C ratio was estimated to be 1.042, NPV was estimated to be KRW 1.93 billion, and IRR was estimated to be 2.1% which means that profitability was secured.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.3
• /
• pp.361-364
• /
• 1999

The binding characteristics of Anagrapha falcifera nuclear polyhedrosis virus (AtNPV) to Spodoptera frugiperda 21 (Sf21) cells were investigated. The cells displayed an affinity of 4.7×10/sup 10/M/sup -1/ with about 3,300 binding sites per cell. The biochemical nature of the AfNPV-binding sites on the cell surface was also partially identified. Our findings suggest that the binding-site moiety has a glycoprotein component, but that the direct involvement of oligosacccharides containing N-acetylglucosamine or sialic acid residues in binding is unlikely, and that AfNPV entry into Sf21 cells may be via receptor-mediated endocytosis.

• Journal of Sericultural and Entomological Science
• /
• v.40 no.1
• /
• pp.52-59
• /
• 1998

For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.