• 수산경영론집
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• 제29권2호
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• pp.199-216
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• 1998

An economic appraisal of a proposed marine ranching project is analysed using capital budgeting model such as net present value(NPV) and internal rate of return( IRR) as well as sensitivity analysis and goal seeking model. Of the factors for economic appraisal, direct benefits are to be determined by estimated harvest, prices and costs incurred by catching fishes, and indirect benefits include the additional economic effect of recreational fishing. And judging the worth of these project options depends upon the choice of discount rate of which 8.5% is recommended here. On the basis of estimated production, prices and costs the project is expected to yield NPV=615 million won and IRR=8.8%, which is quite accepted for an economic feasibility, under the first scenario, and NPV= -127 million won and IRR=7.93%, which is rejected, under the second scenario. Sensitivity analysis has been performed by calculating the switching value and sensitivity indicator in respect of the main project parameters. The results suggest that the project NPV and IRR are especially sensitive to fishes(rock fish and other rock fish) prices and fixed costs. Finally goal seeking analysis is carried out in order to reach a desired level of performance like NPV=0 in respect of the amount of hatchery-reared juverniles, the prices and the discount rate.

• 한국잠사곤충학회지
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• 제33권1호
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• pp.21-26
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• 1991

Bm 배양세포주(BmN4)에서 BmNPV의 다각체 단백질 합성과 DNA복제에 대한 실험결과는 다음과 같다. 1. BmNPV는 insect Grace's medium에서 배양되고 있는 BmN4 세포에서 NOV나 DNA로 감염시킨 경우 모두 잘 증식되었으며, 도립현미경 관찰 결과 접종 후 48시간이 경과하면서 다각체가 형성되기 시작하였다. 2. 또한 전자현미경으로 핵내 형성된 다각체와 협성중인 nucleocapsid 다발을 관찰하였으며, 바이러스 입자는 SNPV로 존재하였다. 3. Western blot분속에 의한 다각체 단백질의 합성은 접종 후 18시간부터 관찰되었으며, 다각체 단백질의 분자량은 30kd이었다. 4. Wild type BmNPV DNA와 Bm 세포(BmN4)에서 NOV 및 DNA 감염에 의해 형성된 바이러스 DNA간의 제한효소 패턴은 차이가 없었다.

• 한국환경과학회지
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• 제20권1호
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• pp.119-126
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• 2011

In this study, reaction model and reactions rate accelerated by o-iodosobenzoate ion(IB$^{\ominus}$) on hydrolysis reaction of p-nitrophenyl valate(NPV) using ethyl tri-octyl ammonium mesylate(ETAMs) for quaternary ammonium salts, the phase transfer catalysis(PTC) reagent, were investigated. The effect of IB$^{\ominus}$ on hydrolysis reaction rate constant of NPV was weak without ETAMs solutions. Otherwise, in ETAMs solutions, the hydrolysis reactions exhibit higher first order kinetics with respect to the nucleophile, IB$^{\ominus}$, and ETAMs, suggesting that reactions are occurring in small aggregates of the three species including the substrate(NPV), whereas the reaction of NPV with OH$^{\ominus}$ is not catalyzed by ETAMs. Different concentrations of NPV were tested to measure the change of rate constants to investigate the effect of NPV as substrate and the results showed that the effect was weak. This means the reaction would be the first order kinetics with respect to the nucleophile. This behavior for the drastic rate-enhancement of the hydrolysis is referred as 'Aggregation complex model' for reaction of hydrophobic organic ester with o-iodosobenzoate ion(IB$^{\ominus}$) in hydrophobic quarternary ammonium salt(ETAMs) solutions.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권2호
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• pp.157-162
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• 2010

Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the $(His)_6$-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified $(His)_6$-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.

• 설비공학논문집
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• 제20권5호
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• pp.321-326
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• 2008

This paper presents and analyzes the effects of on-grid electricity cost, fuel price and initial capital cost of a CHP system, on the optimum DG and AC capacity and NPV, by using the ORNL CHP Capacity Optimizer, which was applied to a library in a university. By considering the current domestic energy cost and initial capital cost, it is shown that the installation and operation of the CHP system is not economical. However, with the current domestic CHP installation cost and fuel price, the NPV achieved by the installation of CHP system is greater when the on-grid electricity price is a factor of ${\times}1.5$ the present value. Regarding the initial capital cost of the CHP system, the reduction of the DG cost is much more economical than that of the AC cost, with respect to NPV. Electricity cost and fuel price have opposite effects on NPV, and NPV is more sensitive to an increase of the electricity cost than an increase of the fuel price.

• 한국지리정보학회지
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• 제16권4호
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• pp.91-105
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• 2013

본 연구에서는 강원권 및 동남권에 해당하는 동해와 남해일부 해역을 대상으로 파력발전 시스템을 도입함으로서 기대할 수 있는 잠재성을 평가하였다. 연구지역 해역에 750kW급 파력발전기 28대를 설치하는 것을 가정하고, 미국 국립해양대기청(NOAA)의 NWW3(Noaa Wave Watch III) 모델로부터 구축된 평균 유의파고 및 첨두파주기 자료와 스텐포드 대학과 미네소타 대학이 공동 개발한 InVEST 소프트웨어를 이용하여 연간 전력 생산량과 경제적 효과를 분석하였다. 분석 결과 연구지역에서의 발전 전력량은 최대 1,207MWh/year, 최소 163MWh/year로 산정되었으며, 연안보다는 육지로부터 먼 해역으로 갈수록 발전 전력량이 점차 증가하는 공간적 분포 패턴을 보였다. 파력발전 시스템의 운영기간을 25년으로 가정하고 시스템 도입을 위해 투입되는 비용과 생산되는 전력의 판매 수익을 함께 고려하여 순현재가치(NPV)를 산정하였다. 그 결과 파력발전 시스템으로부터 생산된 전력을 해저 케이블과 강원권과 동남권 지역의 해안가에 위치한 10개의 발전소들의 설비를 이용하여 내륙으로 공급할 경우에는 NPV가 최대 5,882달러(약 6,600천원), 최소 -63,494달러(약 -71,000천원)인 것으로 분석되었다. 반면, 파력발전 시스템으로부터 생산된 전력을 울릉도와 독도의 전력망으로 공급할 경우, 해저 케이블 설치를 위해 투입되는 초기 비용이 크게 줄어들어 울릉도, 독도 인근 해역에서 NPV 값이 최대 28,095달러(약 31,600천원)까지 증가하는 것을 확인할 수 있었다. 또한, 전력 판매단가가 증가할수록 동해상의 NPV의 손익분기선이 육지 쪽으로 가까워지는 것으로 분석되었으며, 울릉도, 독도 인근 해역에서는 전력 판매단가가 현재 수준보다 100원 상승할 경우 NPV 값이 최대 88,158달러(약 99,000 천원)까지 증가하는 것으로 예측되었다.

• 한국잠사곤충학회지
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• 제40권1호
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• pp.52-59
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• 1998

For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.

• 한국응용곤충학회지
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• 제27권4호
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• pp.211-218
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• 1988

담배거세미나방(Spodoptera litura: SI), 누에(Bombyx mori: Bm) 및 흰불나방(Hyphantria cunea : Hc)으로부터 분리된 핵다각체병 바이러스(nuclear polyhedrosis virus : NPV)의 다각체의 단백질의 특성과 그에 대한 alkaline protease의 영향을 SDS-PAGE 및 혈정학적 방법으로 분석했다. Alkaline protease를 부활화시킨 후 다가체 단백질을 SDS-PAGE한 결과, BmNPV의 경우 30kD, SINPV와 HcNPV는 31kD의 단일 major band 및 이것들의 종합체(polymer)인 57kD와 66kD의 minor band들이 관찰되었다. Alkaline protease의 부활화를 성략한 SI NPV 다각체를 알칼리 용액으로 시간 차이를 두고 처리한 후 SDS-PAGE한 결고, 알칼리 처리시간이 경과함에 따라서 alkaline protease활성에 의해 다각체 단백질이 일정한 pattern으로 저분자화됨이 뚜렷하였다. SINPV 와 BmNPV 다각체 단백질의 항체를 제조하여 SINPV, BmNPV및 HcNPV 다각체 단백질간의 혈정학적 상동성을 이중형역광산법과 Western blot으로 비교한 결과는 3종 모두에서 공통 antigenic determinants의 존재가 인정되었으며 major 다각체 단백질의 종합체 형성과 alkaline protease에 의한 분해를 확인했다.

• Journal of Microbiology
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• 제45권2호
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• pp.133-138
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• 2007

This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via ${\beta}-galactosidase$ expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.

• 한국잠사곤충학회지
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• 제39권2호
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• pp.180-185
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• 1997

To develope the novel baculovirus transfer vector, the p10 gene was cloned from the Bombyx mori nuclear polygedrosis virus (BmNPV) vB2 strain isolated from the B. mori larvae of sericultural farms. The novel transfer vector was constructed by using the p10 gene of BmNPV vB2 strain was 210 bp. The TAAG sequence at the -71 bp of upstream from translation initiator ATG and two polyadenylation signal site at the downstream from terminator TAA were also detected in the p10 gene. The 5' and 3' flanking region of the p10 gene amplified by PCR was cloned into pBluescriptII SK(+) and then transfer vector pBm10 was construceted. The 7.9 kb pBm10 was analysed by restriction enzymes and the map was confirmed. In order to determine the expression of foreign gene of pBm10, $\beta$-galactosidase gene was inserted in the SmaI site of foreign gene cloning site of pBm10. The pBm10 containing $\beta$-galactosidase gene was cotranfected wth genomic DNA of BmNPV vB2 into BmN-4 cells. The recombinant baculovirus expressing $\beta$-galactosidase was also produced polygedra in the infected cells. The results indicated that pBm10 is functional, suggesting that in the baculovirus expression vector system, the recombinant virus produced by pBm10 was effective by oral infection for the producing recombinant proteins in in vivo expression.