• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.775-779
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• 2003

충남 태안소재 순천향대학교 해양연구소의 해양수산연구소에서 probiotics 개발을 궁극적인 목표로 하여 대하양식장에서 대하의 효율적 생산을 위한 생육환경을 조사하였다. 대하의 생장에 영향을 미치는 용존산소, 수체온도, pH, 염도, $NH_4-N$, $NO_2-N$, $NO_3-N$, 클로로필 a를 포함하는 몇가지 환경요인들을 조사하였다. 수 표본에 용존되어 있는 $NH_4-N$, $NO_2-N$, 그리고 $NO_3-N$의 농도는 각각 0.024-0.034 mg/L, 0.03-0.02, 0.004-0.009 mg/L였다. 클로로필 a는 0.002-0.018 $ug/m^3$ 범위에 있었다. 양식장에서 채취한 수 표본에서 다른 세균들의 분포를 알아보기 위하여 marine agar plates에서 분리 계수하였다. 총세균수는 대략 $6.5\;{\times}\;10^4$까지 증가하였다. BIOLOG 시험들 통하여 13가지의 분리 세균들은 Corynebacterium nirilophilus, Clavibacter agropyri, Sphingomonas adhaesiva, Brevundimonas vesicularis, Vibrio parahaemolyticus, Pseudomonas bathycetes, Vibrio tubiashii, Sphingomonas macrogoltabidus, Rhodococcus, rhodochrous, Burkholderia glumae, Corynebacterium urealyticus, Rhodococcus fascians, Psychrobacter immobilis로 각각 동정되었다. 향후 연구는 대하의 효율적인 생산을 위한 정보를 제공하기 위한 병독성이나 환경과 probiotics의 연관성을 규명에 집중 될 것이다.

• 한국환경보건학회지
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• 제23권1호
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• pp.50-54
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• 1997

The removal efficiency of nutrient was investicated by using Glycine max Meer seedling. After budding, Glycine max Merr was raised at darkness for 4 days. During cultivation, the removal efficiency of $NO_2-N+NO_3-N$ was up to 90% with initial concentration of 20-100 ppm. The removal efficiency of PO$_4$-P was up to 80% with initial concentration at 30 ppm, but it was down to 22% and 27% at 40 ppm and 50 ppm. When the removal efficiency of nutrient was compared with alternating 12 hours' light and darkness, the removal efficiency of NO$_2$-N + NO$_3$-N was up to 90% at below 60 ppm. It was not different from each other. But it was particularly low about 62% and 34% at 80 ppm and 100 ppm in alternating 12 hours' light. The removal efficiency of PO$_4$-P was low at alternating 12 hours' light between 10-50 ppm on the whole range. The neutralizing capacity of pH was shown in acidity and alkalinity except strong acidity(below pH 3). The initial pH was neutralized at 6.0-7.7 of pH after 4 days. Particularly, Glycine max Meer seedling that was difference from other water plants, was shown the neutralizing capacity in strong alkalinity.

• 한국환경복원기술학회지
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• 제14권1호
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• pp.121-132
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• 2011

Removal rates of $NO_3$-N and TN in the free-water surface wetland system during winter; December, January, February and March, spring and fall; April, May, October and November, and summer; Jun, July, August and September were investigated. The system was established on floodplain in the downstream reach of the Gwangju Stream in 2008. It measures 50 meters in length and 5.5 meters in width. Iris pseudacorus L. grown in pots for about two years were planted in the system. The water stream was funneled in by gravity and its effluent was discharged back in. Volumes and water quality of inflow and outflow were analyzed from December 2008 to November 2010. The inflow was averaged approximately 350 $m^3/day$ and hydraulic residence time was about 3 hours. Average influent and effluent $NO_3$-N concentration was 3.75 and 3.35 mg/L, respectively and $NO_3$-N retention was amounted to 10.6%. Influent and effluent TN concentration were averaged 4.93 and 4.30 mg/L, respectively and TN abatement reached to 12.9%. One-way ANOVA statistics claimed that the average removal rates of $NO_3$-N and TN during winter, spring and fall, and summer were not always the same (p<0.001). The t-Tests of three pairs among $NO_3$-N removal rates of winter, spring and fall, and summer illustrated that the removal rates of winter ($5.04{\pm}1.94$), spring and fall ($10.53{\pm}2.24$), and summer ($18.61{\pm}2.26$) were significantly different each others (p<0.001). Among TN removal rates, the three pairs of t-Tests of three seasons showed that the removal rates of winter ($5.21{\pm}2.51$), spring and fall ($11.71{\pm}3.12$), and summer ($21.53{\pm}4.86$) were significantly different from each others (p<0.001).

• 한국환경과학회지
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• 제11권7호
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• pp.713-720
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• 2002

In order to provide the basic information on the environmental pollution of Kyungsan province, the contents of Pb, Cd, Cr, Cu, Mn and Zn in soil, stream water, aquatic sediment and groundwater were investigated, and also the values of pH, COD, $KMnO_4-C$,\;NH_3-N,\;NO_2-N,\;NO_3-N$ and $Cl^-$ of stream water and groundwater were determined. The results were as follows. The values of COD, $NH_3-N,\;NO_2-N$ and $NO_3-N$ of the stream waters were very low. The contents of Pb, Cd, Cr, Cu and Zn in the stream waters were respectively at range of $0.014~0.063 mg/{\ell},\;0.004~0.007 mg/{\ell$\mid$, 0~0.045 mg/{\ell},\;0~0.008 mg/{\ell}$\;and\;$0.001~0.175 mg/{\ell}$, and these values were much lower than those of contaminated stream water in Korea. The contents of Cd, Cr, Cu and Zn in the soils were respectively at range of 0.12~O.71 ppm, 0.88~2.65 ppm, 2.86~22.33 ppm and 3.89~26.39 ppm, and these values were much lower than those of ordinary polluted areas in Korea. The contents of Cd, Cr, Cu, As, Zn and Mn in the aquatic sediments were respectively at range of 3.05~3.81 ppm, 14.6~70.6 ppm, 13.74~61.59 ppm, 76.8~465.5 ppm, 12.56~190.83 ppm and 333.3~l188.3 ppm. The values of pH, $KMnO_4-C,\;NH_3-N$, and $NO_3-N$ of the groundwaters were respectively at range of 7.6~8.4, $0~3.95{\ell}$, 0.05~0.15 mg/{\ell}$ and 0.05~0.42 $mg/{\ell}$. The contents of Pb, Cd and Cr in the groundwaters were respectively at range of 0.015~0.061 $mg/{\ell}$, 0.O06~0.009 $mg/{\ell}$ and 0.005~0.045 $mg/{\ell}$.

• 한국수산과학회지
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• 제29권4호
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• pp.456-463
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• 1996

1994년 8월부터 1995년 5월까지 년4회 광도만 내부와 만 중앙부에서 퇴적물과 수층간의 용존무기질소 플럭스의 지역적, 계절적 변화와 그 측정방법 및 1차 생산과의 관계를 조사한 결과는 다음과 같다. 1) $NO_4^+-N$ 플럭스의 계산치 및 실험치는 용출플럭스를 보였고, 만 중앙부에 비해 만 내부에서 높았다. 계절적으로는 8월에 가장 높고 1월과 5월에 가장 낮았다. 2) $NO_{2}^{-}+NO_{3]^{-}-N$ 플럭스의 계산치는 두 지점에서 용출플럭스를 보였으나 실험치는 때때로 침강플럭스가 나타났다. 지역적인 차는 $NO_{4}^{+}-N$와 마찬가지로 만 중앙부에 비해 만 내부에서 높았으나 계절적으로는 10월에 가장 낮고 1월에 가장 높았다. 3) $NO_{4}^{+}-N$ 및 $NO_{2}^{-}+NO_{3}^{-}-N$플럭스의 계산치와 실험치의 지역적, 계절적 변동은 대체로 잘 일치하였으나 실험치가 계산치보다 $2\~3$배 높았다. 또한 $NO_{4}^{+}-N$ 플럭스는 관련환경 인자와 밀접한 관계를 보였으나 $NO_{2}^{-}+NO_{3}^{-}-N$플럭스는 간극수중의 그 농도와 아주 밀접한 관계를 보였다. 4) 퇴적물로부터 수층으로 용출되는 용존무기질소와 해역의 1차생산량과의 관계를 검토한 결과, 광도만 전체 퇴적물로부터 용출되는 용존무기질소가 광도만 전 해역의 1차생산의 약 $25\%\~67\%$정도를 기여할 것으로 추정되었다.

• Journal of Biosystems Engineering
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• 제35권5호
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• pp.343-349
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• 2010

Rapid on-site sensing of nitrate-nitrogen and potassium ions in hydroponic solution would increase the efficiency of nutrient use for greenhouse crops cultivated in closed hydroponic systems while reducing the potential for environmental pollution in water and soil. Ion-selective electrodes (ISEs) are a promising approach because of their small size, rapid response, and the ability to directly measure the analyte. The capabilities of the ISEs for sensing nitrate and potassium in hydroponic solution can be affected by the presence of other ions such as calcium, magnesium, sulfate, sodium, and chloride in the solution itself. This study was conducted to investigate the applicability of two ISEs consisting of TDDA-NPOE and valinomycin-DOS PVC membranes for quantitative determinations of $NO_3$-N and K in hydroponic solution. Nine hydroponic solutions were prepared by diluting highly concentrated paprika hydroponic solution to provide a concentration range of 3 to 400 mg/L for $NO_3$-N and K. Two of the calibration curves relating membrane response and nutrient concentration provided coefficients of determination ($R^2$) > 0.98 and standard errors of calibration (SEC) of < 3.79 mV. The use of the direct potentiometry method, in conjunction with an one-point EMF compensation technique, was feasible for measuring $NO_3$-N and K in paprika hydroponic solution due to almost 1:1 relationships and high coefficients of determination ($R^2$ > 0.97) between the levels of $NO_3$-N and K obtained with the ion-selective electrodes and standard instruments. However, even though there were strong linear relationships ($R^2$ > 0.94) between the $NO_3$-N and K concentrations determined by the Gran's plot-based multiple standard addition method and by standard instruments, hydroponic $NO_3$-N concentrations measured with the ISEs, on average, were about 10% higher than those obtained with the automated analyzer whereas the K ISE predicted about 59% lower K than did the ICP spectrometer, probably due to no compensation for a difference between actual and expected concentrations of standard solutions directly prepared.

• 생물환경조절학회지
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• 제10권1호
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• pp.50-54
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• 2001

잎상추 수경재배시 배양액 내 NO$_3$-N와 NH$_4$-N 비율 및 pH 조절이 엽중 nitrate 함량에 미치는 효과를 구명하였다. NO$_3$-/NH$_4$$^{+}$의 비율 처리는 12:1, 10:3, 8:5이었으며, 8:5 처리구에 pH를 자동적으로 조절해준 처리구조 설정하였다. pH의 변화는 12:1 처리구에서는 실험기간 동안 계속적으로 상승하는 경향을 보였고, NH$_4$$^{+}$의 비율이 높을수록 pH가 상당한 저하하다가 낮은 수준에서 유지되었다. NH$_4$$^{+}$의 비율이 가장 높았던 8:5에 pH를 자동적으로 조절해준 처리구에서는 5.5-6.5 사이의 안정적인 수준으로 pH를 유지하였다. 엽중 nitrate 함량은 전반적으로 12:1 처리구에서는 점점 감소하는 경향을 보였으나, 12:1과 8:5에 pH를 조절해준 처리구에서는 비슷한 양상으로 수확시마다 증가하는 경향을 보였다. 즉, NH$_4$$^{+}$의 비율을 높이고 배양액의 pH를 조절하는 것이 생육 및 엽중 nitrate 함량에 가장 좋은 것으로 나타났다

• 한국토양비료학회지
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• 제22권4호
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• pp.290-295
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• 1989

토양(土壤)pH가 강산성(强酸性)에시 약(弱)알카리성(性)의 분포(分布)를 갖는 8개연초경작지토양종류(個煙草耕作地土壤種類), 고평통(진천, pH4.51), 예산통(진천, pH4.54), 지곡통(음성, pH4.71), 송산통(괴산, pH5.01), 안계통(성주, pH5.34), 반호통(성주, pH5.73), 원곡통(진천, pH5.93) 및 반호통(성주, pH7.70) 등(等)에 대하여 토양수분함양(土壤水分含量)을 최대용수양(最大容水量)의 60%, 배양온도(培養溫度)를 $15^{\circ}C$와 $25^{\circ}C$로 하고 질소무첨가(窒素無添加)와 질소첨가(窒素添加)(연초용복합비료(煙草用複合肥料), 10-10-20, 200mgN/g 첨가(添加))로 구분(區分)하여 8주간(週間) 배양(培養)하고 질산화작용(窒酸化作用)에 대(對)한 토양(土壤)pH 및 온도효과(溫度效果)를 조사(調査)하였다. 또한 배양후(培養後) 축적(蓄積)된 $NO_3-N$함양(含量)과 무비구(無肥區)잎담배수양(收量)과의 상호관계(相互關係)를 검토(檢討)한 결과(結果)는 다음과 같다. 1. 배양온도(培養溫度)에 따른 질산화작용(窒酸化作用)은 토양종류(土壤種類)에 따라 다양(多樣)한 양상을 나타냈으며 대부분(大部分) $25^{\circ}C$조건(條件)이 $15^{\circ}C$조건(條件)보다 현저하게 양호(良好)하였고 배양온도간(培養溫度間) 차이(差異)는 배양(培養) 2주후(週後)부터 뚜렷하였으며 토양(土壤)pH가 증가(增加)할수록 적어지는 경향이었다. 2. 배양(培養) 2주(週) 및 4주후(週後)에 축적(蓄積)된 순(純)$NO_3-N$함양(含量)(N첨가구(添加區)-N무첨가구(無添加區))은 각각(各各)의 배양온도조건(培養溫度條件)에서 토양(土壤) pH 증가(增加)에 따라 증가(增加)되는 정(正)의 상관(相關)을 나타냈다. 3. 토양(土壤)의 질소공급능력(窒素供給能力)을 평가(評價)하기 위한 질소유효도(窒素有效度) 지표(指標)로 이용(利用)되고 있는 배양(培養) 2주후(週後) 축적(蓄積)된 $NO_3-N$ 및 순(純)$NO_3-N$함량으로 무비구(無肥區)잎담배수양(收量)과의 상호관계(相互關係)를 검토(檢討)한 결과(結果) 본포이식후(本圃移植後) 초기생육단계(初期生育段階)의 근권토양온도(根圈土壤溫度)와 비슷한 $15^{\circ}C$조건(條件)은 최적온도(最適溫度)인 $25^{\circ}C$조건(條件)보다 더 밀접한 상관(相關)을 나타냈다.

• 한국초지조사료학회지
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• 제20권2호
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• pp.77-84
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• 2000

Eight-week old perennial ryegrass (Lolium perenne L. cv. Reveille) plants were exposed to different NO3-concentrations or osmotic stress with NaCI. Previously labeled "N was chased during 14 days of non-labeled'NO3 feeding in order to investigate NO3 metabolism in relation to osmoregulation. The short termmeasurement of osmotic potential showed that the extemal concentration of Nos- had not great effect on theosmotic potential, but that osmotic adjustment was observed in NaCl-treated plants. Total uptake of NO 3 - waslargely increased by increasing supply level of NO3 while it was depressed by exposing to osmotic stress.Nitrate reduction increased to more than 29% by increasing extemal NO,- concentration from 1 mM to 10mM. When osmotically stressed with NaCI, nitrate reduction was depressed to about 37% as compared to thecontrol. The decrease in translocation of reduced N into leaves was also observed in NaCl exposed plants. Inthe medium exposed to 10 mM NO,., osmotic contribution of nitrate to cumulative osmotic potential wasdecreased, and it was osmotically compensated with soluble carbohydrate. When osmotically stressed withNaC1, the contribution of chloride was much higher than that of nitrate. The present data indicate that N03-in plant tissues, factually affected by the assimilation of this ion, plays an active role in osmotic regulation incorrelation with other osmotica such carbohydrate and chloride.(Key words : Nitrate metabolism, Osmotic stress, Nitrate supply level, Osmoregulation)ate supply level, Osmoregulation)

• The Korean Journal of Ecology
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• 제28권5호
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• pp.261-264
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• 2005

In the current study, we examined the quality of surface water in ponds at two golf courses, located in southern (Country Club P) and eastern (Country Club B) areas of Korea respectively. Seasonal measurements were made of following physical parameters;pH, dissolved oxygen, biological oxygen demand, chloride, alkalinity, hardness, and nitrogen compounds [$NH_3$-N, $NO_2$-N, $NO_3$-N] and the concentrations of four heavy metals (Cd, Cu, Pb, and Zn) throughout the courses. The pH values were within the alkaline range (7.3 to 9.0), and the biological oxygen demand was generally between 0.5 and 3.1 mg/L. The alkalinity and hardness ranged from 19.1 to 68.5mg/L and 16.1 to 63.6 mg/L, respectively. Nitrogen as ammonium $(NH_3-N)$ was detected in all samples, and the samples had low concentrations of $NO_2-N$ and $NO_3-N$. Cd concentrations were relatively high (to 22.44 mg/L); the highest Cd concentration was observed in the pond in Country Club P. Other metal concentrations were low compared to the Quebec guidelines.