• Title/Summary/Keyword: NJ

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Biocontrol of Tomato Fusarium Wilt by a Novel Genotype of 2,4-Diacetylphloroglucinol-producing Pseudomonas sp. NJ134

  • Kang, Beom-Ryong
    • The Plant Pathology Journal
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    • v.28 no.1
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    • pp.93-100
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    • 2012
  • The rhizobacterium NJ134, showing strong $in$ $vitro$ antifungal activity against $Fusarium$ $oxysporum$, was isolated from field grown tomato plants and identified as $Pseudomonas$ sp. based on 16S ribosomal DNA sequence and biochemical analyses. The antifungal compound purified by gas chromatography-mass spectrometry, infrared, and nuclear magnetic resonance analyses from NJ134 cultures was polyketide 2,4-diacetylphloroglucinol (DAPG). Analysis of the sequence of part of one of the genes associated with DAPG synthesis, $phlD$, indicated that the DAPG producer NJ134 was a novel genotype or variant of existing genotype termed O that have been categorized based on isolates from Europe and North America. A greenhouse study indicated that about $10^8$ CFU/g of soil NJ134 culture application was required for effective biocontrol of Fusarium wilt in tomato. These results suggest that a new variant genotype of a DAPG-producing strain of $Pseudomonas$ has the potential to control Fusarium wilt under the low disease pressure conditions.

Transient Analysis by Using Detailed Modeling of NJ and IJ in Combined Transmission Systems (혼합송전계통에서 NJ 및 IJ 상세모델링이 사용된 과도해석)

  • Chea, Jik-Byoung;Son, Yong-Dae;Lee, Jae-Myeong;Ko, Kwan-Man;Lee, Jong-Beom
    • Proceedings of the KIEE Conference
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    • 2015.07a
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    • pp.381-382
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    • 2015
  • 본 논문은 OF 케이블 지중송전선로가 포함된 혼합송전선로의 정확한 과도해석을 위하여 EMTP상에서 NJ, IJ의 상세모델링을 고려하지 않은 기존 혼합송전선로와 R, L, C 선로정수를 이용하여 NJ, IJ의 상세모델링을 제안하고 이를 고려한 혼합송전선로에서 정상 및 과도해석을 하였다. 이 해석을 통해 접속함 상세모델링을 고려한 해석과 기존 방법의 해석에 차이점을 비교분석하여 OF 케이블 NJ, IJ의 모델링 및 해석의 효용성을 검토하였다. 모델링 및 해석은 EMTP를 이용하였다.

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Mannitol Amendment as a Carbon Source in a Bean-based Formulation Enhances Biocontrol Efficacy of a 2,4-diacetylphloroglucinol-producing Pseudomonas sp. NJ134 Against Tomato Fusarium Wilt

  • Kang, Beom-Ryong
    • The Plant Pathology Journal
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    • v.27 no.4
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    • pp.390-395
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    • 2011
  • Fusarium wilt caused by Fusarium oxysporum has become a serious problem world-wide and relies heavily on chemical fungicides. We selected Pseudomonas sp. NJ134 to develop an effective biocontrol strategy. This strain shows strong antagonistic activity against F. oxysporum. Biochemical analyses of ethyl-acetate extracts of NJ134 culture filtrates showed that 2,4-diacetylphloroglucinol (DAPG) was the major compound inhibiting in vitro growth of F. oxysporum. DAPG production was greatly enhanced in the NJ134 strain by adding mannitol to the growth media, and in vitro antagonistic activity against F. oxysporum increased. Bioformulations developed from growth of NJ134 in sterile bean media with mannitol as the carbon source under plastic bags resulted in effective biocontrol efficacy against Fusarium wilt. The efficacy of the bioformulated product depended on the carbon source and dose. Mannitol amendment in the bean-based formulation showed strong effective biocontrol against tomato Fusarium wilt through increased DAPG levels and a higher cell density compared to that in a glucose-amended formulation. These results suggest that this bioformulated product could be a new effective biocontrol system to control Fusarium wilt in the field.

JACOBSON RADICAL AND NILPOTENT ELEMENTS

  • Huh, Chan;Cheon, Jeoung Soo;Nam, Sun Hye
    • East Asian mathematical journal
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    • v.34 no.1
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    • pp.39-46
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    • 2018
  • In this article we consider rings whose Jacobson radical contains all the nilpotent elements, and call such a ring an NJ-ring. The class of NJ-rings contains NI-rings and one-sided quasi-duo rings. We also prove that the Koethe conjecture holds if and only if the polynomial ring R[x] is NJ for every NI-ring R.

Tissue Specific Gene Regulation of The Anthocyanin Synthesis Regulator Gene R in Maize (옥수수의 색소 발현에 관련된 조직 특이성 조절유전자 R locus에 관하여)

  • 임용표
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.323-347
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    • 1987
  • The R locus of maize in one of several genes that regulate the anthocyanin pigments throughout the body of the plant and seed. The R gene product may regulate pigment deposition by controlling the expression of the flavonoid biosynthetic gene pathway in a tissue-specific manner. To understand the basis for tissue specific regulation and allelic variation at R, the molecular study has been done by cloning a portion of the R complex by transposon tagging with Ac. R specific probe were cloned from the R-nj mutant induced by Ac insertion mutagenesis. From southern analysis of R-r complex using the R-nj probe, the structure of R-r was proposed that R-r containes the three elements, (P)(Q)(S). These elements may organize as the inversion triplication model which (S) sequence was inverted in relation to (P) and (Q). The R-sc derivated from R-mb or R-nj was cloned with R-nj probe, and molecular genetical data showed that R-sc containes tissue specific and tissue nonspecific area, and the sequencing of R-sc are progressed now.

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TCP NJ+: Packet Loss Differentiated Transmission Mechanism Robust to High BER Environments (TCP NJ+ : 높은 BER에 강인한 패킷 손실 원인별 처리기반 전송방식)

  • Kim, Jung-Rae;Lee, You-Ho;Choo, Hyun-Seung
    • Journal of Internet Computing and Services
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    • v.8 no.5
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    • pp.125-132
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    • 2007
  • Transmission mechanisms that include an available bandwidth estimation algorithm and a packet loss differentiation scheme, in general, exhibit higher TCP performance in wireless networks. TCP New Jersey, known as the best existing scheme in terms of goodput, improves wireless TCP performance using the available bandwidth estimation at the sender and the congestion warning at intermediate routers. Although TCP New Jersey achieves 17% and 85% improvements in goodput over TCP Westwood and TCP Reno, respectively, we further improve TCP New Jersey by exploring improved available bandwidth estimation, retransmission timeout, and recovery mechanisms. Hence, we propose TCP New Jersey PLUS (shortly TCP NJ+), showing that under 1% packet loss rate, it outperforms 3% by TCP New Jersey and 5% by TCP Wes1wood. In 5% packet loss rate, a characteristic of high bit-error-rate wireless network, it outperforms other TCP variants by 19% to 104% in terms of goodput even when the network is in bi-directional congestion.

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Antimicrobial and Antioxidant Activities in Different Parts and Cultivars of Broccoli (브로콜리 품종 및 부위에 따른 항균활성과 항산화 효과)

  • Park, Mi Young;Yoon, Moo Kyung;Kwak, Jung-Ho
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.408-414
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    • 2014
  • The antimicrobial and antioxidant activities of different cultivars and parts of broccoli were investigated. The screening of antimicrobial activities for the floret and leaf extracts of 11 cultivars against selected gram positive (Staphylococcus aureus and Listeria monocytogenes) and gram negative bacteria (Escherichia coli O157:H7 and Salmonella enteritidis) were conducted. The AMG cultivar showed the highest antimicrobial activity against S. aureus with the floret and leaf extracts (12.83 and 13.00 mm). Antibacterial activity against L. monocytogenes was strongest with floret extract of NJ cultivar (13.58 mm) and leaf extract of YDR cultivar (13.92 mm). Moreover, the size of inhibition zone against L. monocytogenes was bigger than those of 4 kinds of pathogenic bacteria. Both floret and leaf extracts of Grd cultivar showed the highest antimicrobial activity against E. coli O157: H7, but there was no difference between floret and leaf extracts. Floret extract o f NJ cultivar and leaf extract of NY and 0c cultivars were effective against S. enteritidis whereas leaf extract exhibited better antimicrobial effect than floret extract. These results showed that floret extract of NJ and leaf extract of 0c had the highest antioxidant activity which was 39.90 and 43.64%, respectively. The antioxidant activity of leaf extract was 1.5 times higher than that of floret extract. All cultivars except NJ showed that electron donating ability of leaf extract was higher than that of floret extract.

Effects of Samjung-hwan($S{\={a}}nj{\={i}}ngw{\'{a}}n$) on Obesity and Lipid Metabolism In Rats with High Fat Diet (삼정환(三精丸)이 고지방(高脂肪) 식이(食餌) 흰 쥐의 비만에 미치는 영향)

  • Kim, Ji-Hyung;Kim, Geun-Woo;Koo, Byung-Soo
    • Journal of Oriental Neuropsychiatry
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    • v.20 no.2
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    • pp.47-60
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    • 2009
  • Objectives : This study was performed to show the effects of Samjung-hwan ($S{\={a}}nj{\={i}}ngw{\'{a}}n$) extract on obesity and lipid metabolism of rats fed high fat diet. Methods : Crushed Samjung-hwan($S{\={a}}nj{\={i}}ngw{\'{a}}n$) 245 g was extracted with methyl alcohol. The extract was evaporated under reduced pressure to give 21.8 g. 20 rats were divided into normal, control, and two test groups. For 6 weeks, The normal group rats were supplied with a normal diet. The control group rats were supplied with a high fat diet. The test group 1 rats were supplied with a high fat diet and Samjung-hwan($S{\={a}}nj{\={i}}ngw{\'{a}}n$) extract 250 rug/kg and the test group 2 rats were supplied witha high fat diet and Samjunghwan($S{\={a}}nj{\={i}}ngw{\'{a}}n$) extract 500 mg/kg. Results : 1) The control group rats showed significantly increased weight of peritoneal fat than the nornal group rats. The test group 1 rats did not show significant difference compare to the control group rats, and the test group 2 rats showed significantly decreased weight of peritoneal fat than the control group rats. 2) The control group rats showed significantly increased triglyceride than the nornal group rats. The test group 1 rats showed significantly decreased triglyceride than the control group rats, and the test group 2 rats showed significantly decreased triglyceride than the control group rats. 3) The control group rats showed significantly increased LDL-cholesterol than the nornal group rats. The test group 1 rats showed significantly decreased LDL-cholesterol than the control group rats, and the test group 2 rats did not show significant difference compare to the control group rats. Conclusions : These results suggest that effectiveness of Samjung-hwan($S{\={a}}nj{\={i}}ngw{\'{a}}n$) extract to control the obesity and abnormal lipid metabolism caused by a high fat diet.

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Molecular Phylogeny and Modular Structure of Hybrid NRPS/PKS Gene Fragment of Pseudoalteromonas sp. NJ6-3-2 Isolated From Marine Sponge Hymeniacidon perleve

  • Zhu, Peng;Zheng, Yanling;You, Yurong;Yan, Xiaojun;Shao, Jianzhong
    • Journal of Microbiology and Biotechnology
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    • v.19 no.3
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    • pp.229-237
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    • 2009
  • Among 12 marine bacterial strains from the China coast that exhibited interesting bioactivity (positive for both antimicrobial and cytotoxic activities), only four strains, namely, NJ6-3-1, NJ6-3-2, NB-6, and YTHM-17, had a KS domain or A domain when screened for PKS and NRPS genes using a PCR. Interestingly, two of these strains belonging to Pseudoalteromonas and associated with the marine sponge Hymeniacidon perleve were positive for both PKS and NRPS, whereas the other two strains of Pseudoalteromonas did not have a PKS or NRPS gene. A molecular phylogeny analysis and DGGE analysis of the Pseudoalteromonas sp. indicated that they had a specific affinity with the host marine sponge Hymeniacidon perleve. Furthermore, an analysis of a partial sequence of Pseudoalteromonas sp. NJ6-3-2 isolated from the marine sponge Hymeniacidon perleve obtained from genomic walking using a computational approach indicated a relatively complete PKS module including auxiliary domains (DH, KR, and Cy).

Antiviral Effect of Water Soluble Substance from Elfvingia applanata Alone and in Combinations with Interferons Against Vesicular Stomatitis Virus (New Jersey Serotype) (잔나비걸상 수용성물질의 Vesicular Stomatitis Virus(New Jersey Serotype)에 대한 항바이러스작용과 Interferon과의 병용효과)

  • Rym, Kyo-Hwan;Eo, Seong-Kug;Kim, Young-So;Lim, Jai-Yun;Han, Seong-Sun
    • The Korean Journal of Mycology
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    • v.27 no.2 s.89
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    • pp.175-179
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    • 1999
  • In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [New Jersey serotype, VSV(NJ)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha and gamma were examined on the multiplication of VSV(NJ). EA caused a concentration-dependent reduction in the plaque formation of VSV(NJ) with 50% effective concentration $(EC_{50})$ of 2.10 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha showed more potent effect with CI values of $0.87{\sim}1.59$ for 50%, 70% and 90% effective levels than that with INF gamma with CI values of $1.05{\sim}2.03$.

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