• 한국축산식품학회지
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• 제27권3호
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• pp.357-362
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• 2007

Nine brand populations of Hanwoo cattle were characterized using 11 microsatellite DNA markers. The studied populations were: Ansung, Yangpyang, DaeGwanryeng, Palkongsangkangwoo, Hoengseong, Jangsu, Sumjinkang, Hadong, Nam-hae. The observed heterozygosity, expected heterozygosity, and polymorphism information content were calculated. Allele frequencies were calculated and used for the characterization of each brand population and to study their genetic relationships. Genetic distances were estimated using Nei's DA genetic distance and the resultant DA matrix was used in the construction of phylogenetic trees. The NJ tree showed that Ansung and Yangpyang, Sumjinkang and Jangsu, Namhae and Ha-Dong are closely related and are considered to have undergone genetic exchange within the same locale. This study will contribute to the local Hanwoo brand industry.

• Asian-Australasian Journal of Animal Sciences
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• 제28권1호
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• pp.14-19
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• 2015

The genetic diversity of native chicken populations from Myanmar, Thailand, and Laos was examined by using 102 insertion and/or deletion (indels) markers. Most of the indels loci were polymorphic (71% to 96%), and the genetic variability was similar in all populations. The average observed heterozygosities ($H_O$) and expected heterozygosities ($H_E$) ranged from 0.205 to 0.263 and 0.239 to 0.381, respectively. The coefficients of genetic differentiation (Gst) for all cumulated populations was 0.125, and the Thai native chickens showed higher Gst (0.088) than Myanmar (0.041) and Laotian (0.024) populations. The pairwise Fst distances ranged from 0.144 to 0.308 among populations. A neighbor-joining (NJ) tree, using Nei's genetic distance, revealed that Thai and Laotian native chicken populations were genetically close, while Myanmar native chickens were distant from the others. The native chickens from these three countries were thought to be descended from three different origins (K = 3) from STRUCTURE analysis. Genetic admixture was observed in Thai and Laotian native chickens, while admixture was absent in Myanmar native chickens.

• Fisheries and Aquatic Sciences
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• 제17권4호
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• pp.491-496
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• 2014

A total of six specimens (57.03-100.72 mm in standard length) belonging to the family Diodontidae were collected around Jeju Island, Korea between July and August 2013. On the Basis of morphological and molecular analyses, we identified the specimens as Cyclichthys orbicularis (Bloch, 1785), a new record to the Korean fish fauna. Morphologically, the species is characterized by nine caudal fin rays, immovable spines on the head and body except the caudal peduncle, and small black spots dorsally. Some morphometric characters changed disproportionately with growth, which was a new finding. Caudal peduncle length, body width and preanus length to standard length tend to grow at a rate different from that of overall growth. When the 601 base pairs of mitochondrial DNA cytochrome c oxidase subunit I sequences of our specimens were compared with those of other diodontid fishes, our specimens clustered tightly around C. orbicularis (d = 0.000-0.002) in an NJ tree, with a high bootstrap value (99%). We herein describe the morphological and molecular traits of the specimens, and propose a new Korean name, "gud-ga-si-bok-sok" for the genus Cyclichthys, and "dung-geun-gud-ga-si-bok" for C. orbicularis.

• 생태와환경
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• 제39권2호통권116호
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• pp.265-270
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• 2006

The nucleotide sequence variations of mitochondrial DNA were investigated to understand genetic differentiation for five different Pungitius kaibarae populations at five study sites from selected four streams flowing into the East Sea. The complete sequences of mtDNA control regions of them were determined the constant sites 342 bp and variable sites 183 bp including parsimony infromative sites 122 bp. Based on the phylogenetic tree, five populations were monophyletic unit (97% MPand 100% NJ) and separated two groups (Myongpa-Songhuen stream group and others group). The population of Baebong stream (82nd bp, G-A) consistently formed them of the Jasan stream into a monophyletic unit even though it is closer then Myongpa stream (99th bp, T-C) from the Baebong stream. Further studies on the molecular phylogeny for the primary freshwater fish are needed to establish for the fish biodiversity conservation in mountainous and upland streams.

• Restorative Dentistry and Endodontics
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• 제4권1호
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• pp.11-16
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• 1978

This experimental study was made to investigate the effect of the "Hi-Pol" composite resin on the human dental pulp. 38 cavities of healthy permanent teeth were divided into 5 groups which were used as experimental materials. Group 1: Zinc Oxide-Euginol paste was applied to the cavities as controls $\cdots\;\cdots8$ cases Group 2: "Hi-Pol"*filling with Dycal** base $\cdots\;\cdots9$ cases Group 3: "Hi-Pol" filling without Dycal base $\cdots\;\cdots9$ cases Groud 4: Adaptic*** filling with Dycal base $\cdots\;\cdots6$ cases Group 5: Adaptic filling without Dycal base $\cdots\;\cdots6$ cases The treated teeth were extracted after 1 week, 2 weeks, 3 weeks and 4 weeks and processed for histological study. The results obtained from this experimental study were as follows; 1. The controls applied zinc oxide-eugenol showed the minimal pulp response and group 3 and group 5 showed the most severe pulp response. 2. In group 3 and group 5, the severity of pulp response increased in intensity according to the time elapsed. 3. In group 2 and group 4, the mild pulp response was found in earlier stage and the repairing process could be observed in later stage. * Boo-Pyung Co., Korea ** L. D. Caulk Co., Milford, Del. 19963 *** Johnson and Johnson Co., New Brunswick, NJ 08903.

• 한국균학회지
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• 제47권3호
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• pp.181-186
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• 2019

A designated fungal isolate, KNU-US-1802E was isolated from the soil in Uiseong, Korea. To identify characteristics of the isolate, it was cultured on PDA media for 6 days at $35^{\circ}C$. Colonies on PDA are flat, light gray, dense, with entire margins; reverse dark gray to black, with white margins. Aerial mycelia were smooth-walled, hyaline and 40~42 mm diameter after 6 days at $35^{\circ}C$. Conidia were hyaline, one-celled, ellipsoidal to fusiform, forming long chains with average length ${\times}$ width of $5.0{\pm}0.3{\times}2.9{\pm}0.2{\mu}m$. Molecular analysis indicates that the internal transcribed spacer (ITS) region and partial beta-tubulin (tub2) gene sequence showed 100% and 99% similarities, respectively with Acrophialophora ellipsoidea CGMCC 3.15255 collected from China. Phylogenetic analysis by the neighbor-joining (NJ) method shows that the KNU-US-1802E was clustered with A. ellipsoidea CGMCC 3.15255 in a phylogenetic tree constructed using the concatenated sequences of ITS region and tub2 gene sequences with a high bootstrap value. Based on these findings, the isolate KNU-US-1802E was identified as Acrophialophora ellipsoidea, and this is the first report of this isolate in Korea.

• 한국의학물리학회지:의학물리
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• 제31권4호
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• pp.153-162
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• 2020

Purpose: We aim to evaluate the accuracy and effectiveness of an automatically converted radiation therapy plan between Radixact machines by comparing the original plan with the transferred plan. Methods: The study involved a total of 20 patients for each randomly selected treatment site who received radiation treatment with Radixact. We set up the cheese phantom (Gammex RMI, Middleton, WI, USA) with an Exradin A1SL ion chamber (Standard Imaging, Madison, WI, USA) and GAFCHROMIC EBT3 film (International Specialty Products, Wayne, NJ, USA) inserted. We used three methods to evaluate an automatically converted radiation therapy plan using the features of the Plan transfer. First, we evaluated and compared Planning target volume (PTV) coverage (homogeneity index, HI; conformity index, CI) and organs at risk (OAR) dose statistics. Second, we compared the absolute dose using an ion chamber. Lastly, we analyzed gamma passing rates using film. Results: Our results showed that the difference in PTV coverage was 1.72% in HI and 0.17% in CI, and majority of the difference in OAR was within 1% across all sites. The difference (%) in absolute dose values was averaging 0.74%. In addition, the gamma passing rate was 99.64% for 3%/3 mm and 97.08% for 2%/2 mm. Conclusions: The Plan transfer function can be reliably used in appropriate situations.

• Journal of Species Research
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• 제10권3호
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• pp.276-286
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• 2021

We performed a taxonomic study on Korean Pacificincolidae (Bryozoa: Cheilostomata) with the materials collected from 39 localities(ports, intertidal and subtidal zones) in the coastal seas of South Korea from 1981 to 2020. Korean Mucronella perforata reported by Rho and Seo (1985) turned out to be not Pacificincola perforata, but Primavelans glabricollaris n. sp. based on morphological data. Pacificincola perforata, which was transferred from Mucronella perforata (Okada and Mawatari, 1937), is also added to the Korean fauna. This study is the first to use mitochondrial 16S rRNA gene sequences to study the family Paficincolidae. The NJ analysis also reconfirmed the validity of the genus Primavelans, established on the basis of the early astogeny by De Blauwe in 2006, with the mitochondrial 16S rRNA sequences. The inter-genetic distance among two species belong to the Pacificincolidae was in the range of 13.39±1.83 mm. Primavelans glabricollaris n. sp. is thus distinguished from Pacificincola perforata with both morphological and molecular data. A new species, Primavelans glabricollaris, and a new record, Pacificincola perforata from Korea are herein described in detail and illustrated by scanning electron microscopy.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.733-738
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• 2000

The objective of this study was to describe the nature of the inheritance of jumping as a behavioral trait and to analyze quantitatively the jumping height as a measure of vigor in two subspecies of mice. Two subspecies of mice, Yonakuni wild mouse (Y) and $CF_{{\sharp}1}$ laboratory mouse (C), were used as the parental types. Reciprocal mating between these two subspecies was made to produce subsequently the first and second generations. The first generation was $F_1$ (YC) resulting from $Y\;male{\times}C\;female$, and $F_1{^\prime}$ (CY) from $C\;male{\times}Y\;female$. The second generation $F_2$ (YCYC) was from mating $F_1{\times}F_1$ and $F_2{^\prime}$ (CYCY) from $F_1{^\prime}{\times}F_1{^\prime}$. Individuals were treated with a set of direct current shock apparatus at six weeks of age to evoke jumping. The results showed that the ratio between jumping and non jumping mice (J: NJ) for C was 0%:100% (0:1), which means that all C did not jump throughout the experiment, whereas Y was 68%:32% (2:1); and the $F_1$ and $F_2$ showed 65%:35% (2:1) and 51%:49% (1:1), respectively. All $F_1{^\prime}$ and $F_2{^\prime}$ individuals jumped as indicated by the ratio 100%:0% (1:0) for both these two genetic groups. Of the jumped mice, average height of the first three jumping observed for pooled sexes in Y, $F_1$, $F_2$, $F_1{^\prime}$ and $F_2{^\prime}$ were 19.3 cm, 19.3 cm, 18.0 cm, 19.9 cm and 16.4 cm, respectively. The distribution of jumping height showed a tendency to be a normal distribution. The jumping activity and jumping height may be affected by some major genes and polygenes, respectively.

• PNF and Movement
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• 제16권2호
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• pp.249-257
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• 2018

Purpose: This study aimed to explore the effects of additional trunk exercises on an unstable surface on the balance and walking ability of individuals with chronic stroke. Methods: Sixteen patients with chronic stroke participated in this study. The participants were randomly assigned to two groups: experimental group (n=8) and control group (n=8). All the participants underwent a typical physical therapy program for 30 min a day. Moreover, the experimental group participated in a 30 min trunk exercise program on an unstable surface, whereas the control group participated in a 30 min trunk exercise program on a stable surface. Both groups performed the exercises five times a week for three weeks. The Berg Balance Scale (BBS) was used to measure changes in balance. The gait variables were measured using the GAITRite system (CIR System Inc., Clifton, NJ, USA) to examine changes in walking ability. Results: Both groups showed a significant intragroup improvement in balance, gait speed, cadence, stride length, and double support period (p<0.05). In the intergroup comparisons after the intervention, the experimental group showed significant improvements over the control group in balance, gait speed, cadence, stride length, and double support period (p<0.05). Conclusion: This study applied additional trunk exercises on an unstable surface to chronic stroke patients, and the results showed a significant improvement in the patients' balance and walking abilities. Therefore, trunk exercise on an unstable surface may be applicable as an intervention method to improve the balance and walking ability of chronic stroke patients.