• Nuclear Medicine and Molecular Imaging
• /
• 제42권5호
• /
• pp.383-393
• /
• 2008

목적: 조직 특이 프로모터를 이용하면 특정 암조직내에서만 원하는 치료유전자를 발현시킬 수 있다. 나트륨 옥소 공동 수송체(sodium iodide symporter: NIS) 유전자는 옥소를 섭취하는 특성을 가져 방사성옥소를 이용한 치료용 유전자로 사용될 수 있다. 광학 영상용 유전자인 luciferase (Luc) 유전자를 세포에 이입하면 비침습적으로 유전자가 이입된 세포의 상태를 평가할 수 있다. 본 연구는 간암 특이성을 나타내는 AFP 프로모터에 의해 발현이 조절되는 NIS유전자와 CMV프로모터에 의해 발현되는 Luc유전자를 간암세포에 이입하여 NIS유전자 이입에 의한 방사성옥소 유전자치료의 효과를 알아보고, 종양사멸 정도를 광학 리포터 유전자 발현으로 알아보고자 하였다. 대상 및 방법: AFP enhancer와 GSTP 프로모터를 연결하여 AFP프로모터를 제작하였으며 이를 NIS유전자와 연결하였다. 또한 CMV 프로모터에 조절 받는 Luc 유전자를 동시에 삽입하여 AFP-NIS-CMV-Luc 유전자 발현 벡터를 생산하였다. 실험 대상 세포주로는 간암세포주인 HepG2와 Huh-7 세포와 사람 대장암세포주인 HCT-15 세포를 이용하였다. AFP-NIS-CMV-Luc 발현벡터를 Liposome을 이용해 실험대상 세포주 내로 이입하였으며, 방사성옥소 섭취율과 방사성옥소의 유출량을 측정하였다. 또한 Luciferase 발현 정도를 luminometer로 측정하였으며, clonogenic assay를 통하여 I-131에 대한 세포주에 따른 사멸효과 차이를 알아보았다. AFP-NIS-CMV-Luc 유전자 이입 세포주를 누드마우스에 대퇴부 피하에 주입하여 I-131 축적여부를 감마카메라 영상을 획득하였다. 결과: AFP-NIS-CMV-Luc 유전자 발현 벡터를 제작하였다. AFP-NIS-CMV-Luc 유전자가 이입된 HepG2와 Huh-7 세포의 방사성옥소 섭취율은 유전자 이입이 되지 않은 대조군 HepG2와 Huh-7 세포에 비하여 높았으며, $KClO_4$를 처리시 옥소 섭취가 저해되었다. 대장암 세포주인 HCT-15세포에 AFP-NIS-CMV-Luc유전자를 이입 시 방사성옥소의 섭취률은 증가되지 않았다. 30분간 방사성옥소를 섭취시킨 AFP-NIS-Luc 유전자가 이입된 HepG2와 Huh-7 세포에서의 방사성옥소의 유출반감기는 약 4분과 6분으로 각각 나타났다. AFP-NIS-CMV-Luc 유전자가 이입된 HepG2, Huh-7세포의 Luc 유전자의 발현은 241, 441 $RLU/2\;{\times}\;10^5$ cells로 나타났으며, 대조군 HepG2와 Huh구세포에서의 Luc 유전자의 발현은 74, $RLU/2\;{\times}\;10^5$ cells로 나타났다. HCT-15 세포는 AFP-NIS-CMV-Luc 유전자 이입에 따라 I-131에 의한 세포 사멸능이 증가되지 않았으나, HepG2 및 Huh-7 세포는 FP-NIS-CMV-Luc 유전자 이 입에 따라 I-131에 의한 세포 사멸능이 증가되었으며, Huh-7세포의 경우 0.5mCi의 I-131을 투여한 경우 모든 세포가 사멸하였다. AFP-NIS-CMV-Luc 유전자가 이입된 Huh-7 세포수가 많을수록 방사성옥소 섭취율이 증가하며 luciferase활성도도 높게 나타났다. AFP-NIS-CMV-Luc 유전자가 이입된 Huh-7 세포를 이식한 누드마우스에 I-131 감마카메라 영상에서 종양이식부위에 방사능 축적을 관찰 할 수 있었다. 결론: AFP프로모터의 의하여 NIS유전자가 발현되며, CMV프로모터에 의한 Luc 유전자가 발현되는 벡터를 제작하였으며, 이 벡터를 이입한 경우 간암세포에서만 I-131의 세포 독성이 증가하는 효과를 나타내었다. 또한 Luc유전자를 이용하여 비침습적인 광학 영상으로 세포사멸 효과를 확인할 수 있었다. 간암특이 프로모터에 조절되는 치료 유전자와 광학리포터 유전자를 한 벡터에 동시에 이입하면 간암 특이 유전자 치료와 그 치료효과를 비침습적으로 평가할 수 있을 것으로 생각된다.

• 한국영어학회지:영어학
• /
• 제2권4호
• /
• pp.519-536
• /
• 2002

This paper is to provide an analysis of the aspectual nature of naked infinitives(NIs) of English perceptual reports. Higginbotham(1983) proposes that only ‘active’ or ‘transient’ verbs can be used as NIs of English perceptual reports, but ‘state’ verbs cannot. This is called “confinement to the active or transient”. But there will be one problem when NIs of English perceptual reports are negatives. This is why the meaning of negative NIs of English perceptual reports may be static, which isn't in line with “confinement to the active or transient”. There are similar problems with ‘state’ NIs of English perceptual reports. In this paper some of these problems can be solved with the “Event Structure” of Pustejovsky(1991). According to Pustejovsky(1991), ‘process’ and ‘transition’ verbs have sub-eventual individuation units, but state verbs have no such units. It is the units that make it possible for ‘process’ and ‘transition’ to be perceived. As a result ‘process’ and ‘transition’ verbs are suitable for NIs of English perceptual reports. Nevertheless, there are still some problems unsolved. Moreover some state verbs are grammatically used as NIs of English perceptual reports. This paper proposes that these state verbs have “potentiality of transition”, and the potentiality comes from only the combination of the two parts of the complement - the subject and the naked infinitive. And the potentiality causes the “Event Structure” of Pustejovsky(1991) to be modified. And this modification makes it possible for us to explain aspect of NIs of English Perceptual Reports. It is concluded with some implication for Korean learners of English as a Foreign Language.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권1호
• /
• pp.104-115
• /
• 2003

A series of experiments was carried out to determine the possibility for the non-ionic surfactant (NIS) as a feed additive for ruminant animals. The effect of the NIS on (1) the enzyme distribution in the rumen fluids of Hereford bulls, (2) the growth of pure culture of rumen bacteria and (3) rumen anaerobic fungi, (4) the ruminal fermentation characteristics of Korean native cattle (Hanwoo), and (5) the performances of Holstein dairy cows were investigated. When NIS was added to rumen fluid at the level of 0.05 and 0.1% (v/v), the total and specific activities of cell-free enzymes were significantly (p<0.01) increased, but those of cell-bound enzymes were slightly decreased, but not statistically significant. The growth rates of ruminal noncellulolytic species (Ruminobacter amylophilus, Megasphaera elsdenii, Prevotella ruminicola and Selenomonas ruminantium) were significantly (p<0.01) increased by the addition of NIS at both concentrations tested. However, the growth rate of ruminal cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens) were slightly increased or not affected by the NIS. In general, NIS appears to effect Gram-negative bacteria more than Gram-positive bacteria; and non-cellulolytic bacteria more than cellulolytic bacteria. The growth rates of ruminal monocentric fungi (Neocallimastix patriciarum and Piromyces communis) and polycentric fungi (Orpinomyces joyonii and Anaeromyces mucronatus) were also significantly (p<0.01) increased by the addition of NIS at all concentrations tested. When NIS was administrated to the rumen of Hanwoo, Total VFA and ammonia-N concentrations, the microbial cell growth rate, CMCase and xylanase activities in the rumen increased with statistical difference (p<0.01), but NIS administration did not affect at the time of 0 and 9 h post-feeding. Addition of NIS to TMR resulted in increased TMR intake and increased milk production by Holstein cows and decreased body condition scores. The NEFA and corticoid concentrations in the blood were lowered by the addition of NIS. These results indicated that the addition of NIS may greatly stimulate the release of some kinds of enzymes from microbial cells, and stimulate the growth rates of a range of anaerobic ruminal microorganisms, and also stimulate the rumen fermentation characteristics and animal performances. Our data indicates potential uses of the NIS as a feed additive for ruminant animals.

• 대한의생명과학회지
• /
• 제12권3호
• /
• pp.171-176
• /
• 2006

Sodium iodide symporter (NIS) plays a key role in thyroid hormone production by efficiently accumulating iodide from the circulating blood into the thyocytes, and this is done against an electrochemical gradient. Thyroid transcription factor-l (TTF-l) is a homeodomain-containing protein expressed in embryonic diencephalons, thyroid, and lung and has been found to bind to thyroid specific promoters and to activate their transcriptional activity. TTF-l may be one of the factors capable of activating NIS gene expression in the thyroid gland, thus it accounts for the lower levels of NIS gene expression that are seen in the extrathyroidal tissues. However, a high frequency of TTF-l expression has been observed, especially in primary lung adenocarcinoma. The present study was undertaken in order to elucidate the relationship between the expression of NIS and TTF-l in primary lung adenocarcinoma. Immunohistochemical studies for NIS and TTF-l were performed in 64 primary lung adenocarcinomas. Immunoreactivities for NIS and TTF-l were found in 49 (76.6%) and 45 (70.3%) out of 64 cases, respectively. Forty-one (83.7%) of the 49 cases with positive NIS immunoreactivity showed positive TTF-l expression, whereas 11 (73.3%) of the 15 cases with negative NIS immunoreactivity showed negative TTF-l expression (P<0.05). So the NIS expression was significantly associated with the TTF-l expression. These findings suggest that TTF-l may be one of the factors capable of activating NIS gene expression in primary lung adenocarcinoma. Further studies are needed to define the relation between NIS and TTF-l for examining the mechanisms of tissue-specific NIS expression.

• 해양환경안전학회:학술대회논문집
• /
• 해양환경안전학회 2007년도 춘계학술발표회
• /
• pp.121-126
• /
• 2007

지난해 국제해사기구 (IMO)의 MSC 는 NAV 및 COMSAR 전문위원회에 E-Navigation 에 대한 전략 개발을 지시하였다. 효율적이고 안전한 선박 운항을 위한 E-navigation 서비스 구현을 위해서 해상 정보와 정보체계에 대한 이해가 선행되어야한다. 본 연구에서는 현재 범유럽적 차원에서 진행 중인 MarNIS 의 정보체계와 정보 에이전트에 대해서 살펴보았다. MarNIS 는 에이전트의 정보 창구로서의 Single Window와 각 시스템 말단에서 데이터 변환기로서의 MarNIS 노드에 의해 범유럽의 해상정보체계를 수립하고 있다. 정보 에이전트들이 계층적으로 구조화되고 통합된 해상 정보체계를 기반으로 각종 해상정보를 종합 분석하여 해상교통 지원 서비스와 해상안전 지원 서비스를 구현할 수 있다.

• 환경정책연구
• /
• 제1권1호
• /
• pp.25-45
• /
• 2002

Opening of trade relationships through an increasing number of international free trade agreements and the now defunct General Agreement on Tariffs and Trade has resulted in an increase the number of the species being exchanged in the world. In the last 20 years, international environmental laws have multiplied and a number of treaties address harmful non-indigenous species (NIS) directly with specific provisions, while other treaties deal with related environmental issues and indirectly affect international regulation of NIS; however, such treaties are weak due to lack of enforceability. From the stand point of national law, many countries including the USA, Australia and New Zealand enforce national laws and regulations to protect biological resources. Typical strategies include : 1) strengthening quarantines to prevent unintentional and illegal introduction of harmful NIS, and 2) developing technologies for managing harmful NIS. However, the recent international trend for managing NIS has shifted. In 2002, the Bonn Guidelines on Access to Genetic Resources and Fair and Equitable Sharing of the Benefit Arising out of their Utilization was adopted at the 6th Conference of the Parties to the Convention on Biodiversity. One major issue highlighted in the document is that "there is no more free of charge to get a biological resource from other countries". The Bonn guidelines will affect international and national NIS regulatory systems because the NIS is a potentially disrupts ecosystems as well as native species. A number of impacts are expected including the revamping of national biodiversity policy regimes in many countries in the world. In particular, the ROK, which is not very biologically diverse, has to evolve national laws to protect valuable ecosystems from NIS. In the meanwhile, national rights of using beneficial indigenous and non-indigenous species as biological resources should be considered through the investigation and national registration of NIS around the world for the promotion of the biotech industry.

• Asian-Australasian Journal of Animal Sciences
• /
• 제22권7호
• /
• pp.993-1004
• /
• 2009

Three experiments were conducted to determine the effects of non-ionic surfactant (NIS) supplementation on ruminal fermentation, nutrient digestibility and performance of beef steers fed high-roughage diets. The objective of experiment 1 was to investigate the effects of NIS supplementation on in vitro ruminal fermentation of cultures administered with corn and barley as grain substrate and rice straw and timothy hay as roughage substrate. The in vivo ruminal fermentation, nitrogen balance and digestibility of nutrients were also examined with steers fed a high-roughage diet in experiment 2. The aim of experiment 3 was to determine the responses to NIS of growing steers fed a high-roughage diet. In experiment 1, ammonia nitrogen concentration for NIS supplementation was higher (p<0.05) than for the control with all substrates. However, concentrations of total volatile fatty acid (VFA), acetate, butyrate and valerate of the incubated roughage substrates, rice straw and timothy hay, were higher (p<0.05) for NIS supplementation than for the control whereas VFA concentrations in the cultures of corn and barley were unaffected. These results indicated that effects of NIS on ruminal fermentation are diet dependent, specifically on roughage sources. In experiment 2, ruminal pH of steers supplemented with NIS was lower (p<0.05) than the control. Ruminal concentrations of ammonia nitrogen, acetate, total VFA and urinary concentrations of purine derivatives were increased (p<0.05) by NIS supplementation. In experiment 3, supplementation of NIS increased (p<0.05) intakes of total feed and corn silage, average daily gain, and feed efficiency of growing steers although they varied depending on supplementation level. Due to the roughage-specific feature of NIS effects, NIS appears to enhance ruminal fermentation of fibrous parts of feeds and, consequently, performance of steers fed a high-roughage diet.

• 위성통신과 우주산업
• /
• 제12권1호통권27호
• /
• pp.74-78
• /
• 2004

노무현 대통령은 지난 7월 30일 청와대에서 제51회 국정과제회의를 주재하고 혁신주도형 경제성장구조로의 전환을 위한 "국가기술혁신체계(NIS : National Innovation System)" 구축 방안을 확정하였다. 이날 확정 발표된 국가기술혁신체계(NIS) 구축방안 내용이다.

• ETRI Journal
• /
• 제33권3호
• /
• pp.320-325
• /
• 2011

This paper considers the effects of simultaneous correlated multipath fading and shadowing on the performances of a signal-to-interference ratio (SIR)-based dual-branch selection combining (SC) diversity receiver. This analysis includes the presence of cochannel interference. A generalized fading/shadowing channel model in an interference-limited correlated fading environment is modeled by generalized-K distribution. Closed-form expressions are obtained for probability density function and cumulative distribution function of the SC output SIR, as well as for the outage probability. Based on this, the influence of various fading and shadowing parameter values and the correlation level on the outage probability is examined.

• 성인간호학회지
• /
• 제25권4호
• /
• pp.365-376
• /
• 2013

Purpose: The purpose of this study was to construct, develop, and apply a nursing information system (NIS) using NANDA-NOC-NIC linkage in medical-surgical nursing units. Methods: This study consisted of three phases which were the construction of the database, development of the NIS, and application of the NIS. To construct the database, a questionnaire and nursing record review by an expert group were used. Collected data were analyzed by the SPSS/WIN 13.0 program. Results: In first phase, the database was made up of 50 nursing diagnoses, 127 nursing outcomes and 300 nursing interventions. In the second phase, NIS was developed according to its flow diagram and then tested. In the third phase, the developed NIS was applied to 130 inpatients. Nursing diagnoses frequently used were acute pain, delayed surgical recovery, and deficient knowledge (specify). Nursing outcomes for a nursing diagnosis of 'acute pain' were identified as pain control, pain level and comfort level. Nursing interventions for the nursing outcome 'pain control' were pain management, patient controlled analgesia assistance and medication management. Conclusion: The results of this study will facilitate the use of the newly proposed NIS in nursing practice and provide a guideline for evidence-based nursing.