• Title/Summary/Keyword: NGL

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Rapid Identification of Candida albicans Using Colorimetric Method

  • Kim, Shin Young;Park, Hun-Hee
    • Korean Journal of Clinical Laboratory Science
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    • v.45 no.4
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    • pp.149-153
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    • 2013
  • Candidiasis is a fungal infection of the most common causes; generally, opportunistic infections occur often in patients with weakened immune systems. Because of high rates in fungal infection patients and increasing frequency of being isolated from clinical materials, quickly identifying of Candida albicans is critical. By identifying 404 yeast cell strains of referred samples via API 20C kits, NGL and PRO tests and Germ tube (GT) test were conducted and compared. In the 3.0 McFarland yeast cells, 0.1% ${\rho}-nitrophenyl-N-acetyl-{\beta}-D-galactosaminide$ (NGL) and 0.04% ${\small{L}}$-proline ${\beta}$-naphtylamide (PRO) were each put in test tubes and incubated at $35^{\circ}C$ for 15, 30, 60 and 90 minutes. Afterwards, 1 drop of 2% NaOH was applied, and if the color turned yellow; it was positive for NGL test. Afterwards, 1% ${\rho}$-dimethylaminocinnamaldehyde was applied, and if the upper layer turned pink or red, it was positive for PRO test. NGL and PRO tests were conducted for all C. albicans and identified accurately within 30 minutes. In NGL, PRO test, false-positive, negative were not seen, whereas, GT test showed false-positive in 1 strain and false-negative in 3 strains. Therefore, sensitivity and specificity of NGL, PRO tests were 100% and 99.5%, respectively, and positive and negative predictive rate were 99.5% and 100%, respectively. However, GT test sensitivity and specificity were 98.5% and 99.5%, respectively, and positive and negative predictive rates were 99.5% and 98.5%, respectively. In conclusion, NGL, PRO tests are better than GT tests for sensitivity and specificity, therefore, these reliable tests will be useful in clinical laboratories.

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Process Design and Analysis of BOG Re-liquefaction System with Pre-liquefaction of NGL (NGL 분리식 BOG 재액화 공정 고안 및 해석)

  • Yun, Sang-Kook
    • Journal of the Korean Institute of Gas
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    • v.19 no.3
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    • pp.32-37
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    • 2015
  • The boil-off-gases(BOG) in cryogenic LNG storage tanks are generating continuously due to the heat leakage and need to be re-liquefied by the effective way. As the present method to reliquefy BOG is using LNG cold energy to be supplied after low pressure primary pump, the demand of LNG flow rate should be over 10 times of BOG produced rate to reliquefy it. This research invented new effective re-liquefaction system having only 3~4 times of LNG flow rate against unit BOG, that the pre-liquefaction process of NGL and the use of high pressure LNG cold energy after secondary pump. By the analysis, it could be high efficient reliquefying system for all amount of BOG treatment even during the summer time, and improvement of operation safety and efficiency of LNG terminal.

Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I

  • Xiao, Yongkun;Liu, Chunying;Im, Wan-Teak;Chen, Shuang;Zuo, Kangze;Yu, Hongshan;Song, Jianguo;Xu, Longquan;Yi, Tea-Hoo;Jin, Fengxie
    • Journal of Ginseng Research
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    • v.43 no.2
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    • pp.186-195
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    • 2019
  • Background: Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from Aspergillus niger g.848. Methods: NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR. Results: All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred through Rb3${\rightarrow}$C-Mx1${\rightarrow}$C-Mx; when reacted for 1 h, Rb3 decreased from 27.1% to 9.82 %, C-Mx1 increased from 15.5% to 32.3%, C-Mx was produced to 6.46%, finally into C-Mx and a small amount of C-K. When reacted for 1.5 h, all the Rb1, Rd, and Gyp17 were completely reacted, and the reaction intermediate F2 was produced to 8.25%, finally into C-K. The main reaction of Rc (13.8%) occurred through Rc${\rightarrow}$C-Mc1${\rightarrow}$C-Mc${\rightarrow}$C-K. The enzyme barely hydrolyzed the terminal xyloside on 3-O- or 20-O-sugar-moiety of the substrate; therefore, 9.43 g C-Mx, 6.85 g C-K, 4.50 g R7, and 4.71 g Fc (hardly separating from the substrate) were obtained from 50 g NGL ginsenosides by the crude enzyme reaction. Conclusion: Four monomer ginsenosides were successfully produced and separated from NGL ginsenosides by the enzyme reaction.

effect of Nif A Overproduction on the Improvement of Nitrogenase Activity of Klebsiella oxytoca Strains (Nif A의 Overproduction에 의한 Klebsiella oxytoca 균주의 질소 고정력 증진 효과)

  • 서현창;유익동
    • The Korean Journal of Food And Nutrition
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    • v.5 no.2
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    • pp.137-143
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    • 1992
  • As a research for developing biofertilizers, Klebsiella oxytoca, an associative nitrogen fixer in the rhizosphere of rice plant in the soil of paddy field, was subjected to molecular breeding. The results obtained were as followings. 1). By transforming pbIC71A, Nif A overproducing plasmid, into Klebsiella oxytoca NGl3, Klebsiell6f oxytoca SH3l, and Klebsiella oxytoca SH161, nitrogenase activities in the absence of nitrogen source in the medium were increased 6.4, 17.2, and 13.5 times, respectively, in comparison with the parent strains. 2). Nitrogenase activity of Klebgiella oxytoca NGl3, Klebsiella oxytoca SH3l, and Klebsiella oxytoca SH161 was completely repressed In the presence of 15mM NH4+. But, nitrogenase activities of Klebsiella oxytoca NGl3/PMC71A, Klebsiella oxytoca SH3l /PMC71A, and Klebsiella oxytoca SH 161/pMC714 harboring PMC71A, were 13.7%, 7.7%, and 6.2% of the nitrogenase activities in the absence of nitrogen source in the medium, respectively.

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NGL-전환기의 세계가스정제업계

  • Korea Petroleum Association
    • Korea Petroleum Association Journal
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    • no.8 s.42
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    • pp.51-55
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    • 1984
  • 이 글은 미국가스정제업자협회의 전무이사인 Ron Cannon씨가 최근 Oil & Gas Journal지에 발표한 글을 발췌ㆍ번역한 것이다. <편집자 주>

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EUVL Mask Defect Isolation and Repair using Focused Ion Beam (Focused Ion Beam을 이용한 EUVL Mask Defect Isolation 및 Repair)

  • 김석구;백운규;박재근
    • Journal of the Semiconductor & Display Technology
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    • v.3 no.2
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    • pp.5-9
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    • 2004
  • Microcircuit fabrication requires precise control of impurities in tiny regions of the silicon. These regions must be interconnected to create components and VLSI circuits. The patterns to define such regions are created by lithographic processes. In order to image features smaller than 70 nm, it is necessary to employ non-optical technology (or next generation lithography: NGL). One such NGL is extreme ultra-violet lithography (EUVL). EUVL transmits the pattern on the wafer surface after reflecting ultra-violet through mask pattern. If particles exist on the blank mask, it can't transmit the accurate pattern on the wafer and decrease the reflectivity. It is important to care the blank mask. We removed the particles on the wafer using focused ion beam (FIB). During removal, FIB beam caused damage the multi layer mask and it decreased the reflectivity. The relationship between particle removal and reflectivity is examined: i) transmission electron microscope (TEM) observation after particle removal, ii) reflectivity simulation. It is found that the image mode of FIB is more effective for particle removal than spot and bar mode.

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A Study on the Application of Copyright for Digital Maps in Korea (우리나라 수치지도의 저작권 적용방안에 관한 연구)

  • Heo, Min;Seo, Chang-Wan;Yoon, Ha-Su;Park, Hong-Gi;Choi, Yun-Soo
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.6_2
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    • pp.653-663
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    • 2007
  • The purpose of this study is to suggest concrete approaches of copyright application among various map price policies to establish the system of a digital map market, to revitalize its market, and to give the public better map services. To do this, we analyzed copyright law in Korea and the copyright protection of digital map in foreign countries firstly, investigated the applicability of copyright to the map market in Korea, analyzed the effect on this market, and suggested an direction of the revision for the Surveying law and the National Geographic Information (NGlS) law lastly. We drew several conclusions. Firstly, the protection of the rights of database makers is the best approach to protect digital maps in korea. Secondly, we need to adopt copyright to the Surveying law and the National Geographic Information Systems (NGlS) law. Lastly, establishing a copyright trust organization to improve the quality of digital maps and to manage various affairs is needed.

An Optimization Study on the NGL Recovery Process Using Turbo-expander (터보 팽창기를 활용한 NGL 회수공정 최적화에 대한 연구)

  • Kim, Yu-Mi;Cho, Jung-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.3
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    • pp.1473-1478
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    • 2011
  • In this study, simulation and optimization works for a demethanizer column have been performed to obtain ethane and heavier products from a pretreated natural gas stream. Pretreated natural gas feed stream is partially condensed after being precooled by exchanging heat with demethanizer top vapor stream and by using an external refrigeration cycle with a propane refrigerant. Vapor stream is furtherly cooled and partially condensed through a turbo-expander and the power generated from the expansion of turbo-expander was delivered to the compressor for the residue gas compression. Liquid stream is being cooled by Joule-Thomson expansion valve and is fed to the middle section of the demethanizer. Ethane recovery percent for feed natural gas was set to 75% and methane to ethane molar ratio was fixed as 0.015. Propane refrigeration heat duty was reduced by splitting the feed stream and to exchange heat with side reboiler.