• Title/Summary/Keyword: NFA

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Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri

  • Jeong, Seok-Ryoul;Kang, Su-Yeon;Lee, Sang-Chul;Song, Kyoung-Ju;Im, Kyung-Il;Shin, Ho-Joon
    • Parasites, Hosts and Diseases
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    • v.42 no.1
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    • pp.35-40
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    • 2004
  • The nfa 1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia-specific localization by immunocytochemistry in the previous study. Based on the idea that the pseudopodia-specific Nfa1 protein mentioned above seems to be involved in the pathogenicity of N. fowleri, we observed the effect of an anti-Nfa1 antibody on the proliferation of N. fowleri trophozoites and the cytotoxicity of N. fowleri trophozoites on the target cells. The proliferation of N. fowleri trophozoites was inhibited after being treated with an anti-Nfa1 polycional antibody in a dose-dependent manner for 48 hrs. By a light microscope, CHO cells co-cultured with N. fowleri trophozoites (group I) for 48 hrs showed severe morphological destruction. On the contrary, CHO cells co-cultured with N. fowleri trophozoites and anti-Nfa1 polyclonal antibody (1:100 dilution) (group II) showed less destruction. In the LDH release assay results, group I showed 50.6% cytotoxicity, and group II showed 39.3%. Consequently, addition of an anti-Nfa1 polyclonal antibody produced a decreasing effect of in vitro cytotoxicity of N. fowleri in a dose-dependent manner.

An Efficient Complex Event Detection Algorithm based on NFA_HTS for Massive RFID Event Stream

  • Wang, Jianhua;Liu, Jun;Lan, Yubin;Cheng, Lianglun
    • Journal of Electrical Engineering and Technology
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    • v.13 no.2
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    • pp.989-997
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    • 2018
  • Massive event stream brings us great challenges in its volume, velocity, variety, value and veracity. Picking up some valuable information from it often faces with long detection time, high memory consumption and low detection efficiency. Aiming to solve the problems above, an efficient complex event detection method based on NFA_HTS (Nondeterministic Finite Automaton_Hash Table Structure) is proposed in this paper. The achievement of this paper lies that we successfully use NFA_HTS to realize the detection of complex event from massive RFID event stream. Specially, in our scheme, after using NFA to capture the related RFID primitive events, we use HTS to store and process the large matched results, as a result, our scheme can effectively solve the problems above existed in current methods by reducing lots of search, storage and computation operations on the basis of taking advantage of the quick classification and storage technologies of hash table structure. The simulation results show that our proposed NFA_HTS scheme in this paper outperforms some general processing methods in reducing detection time, lowering memory consumption and improving event throughput.

Comparison of Surgical Outcomes of Laparoscopic Adrenalectomy Between Patients With Nonfunctioning Adrenal Adenomas and Subclinical Cushing Syndrome: A Single-Center Experience

  • Kang, Minyong;Baek, Minki;Lee, Jonghoon;Jeong, Byong Chang
    • The Korean Journal of Urological Oncology
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    • 제15권3호
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    • pp.137-142
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    • 2017
  • Purpose: To examine and compare the perioperative outcomes of laparoscopic adrenalectomy (LA) and to determine its efficacy in patients with nonfunctioning adrenal adenomas (NFA) and subclinical Cushing syndrome (SCS). Materials and Methods: We retrospectively analyzed the clinical data obtained from 79 consecutive patients who underwent LA for the treatment of either NFA (n=35) or SCS (n=44) between 2011 and 2016. All patients had undergone computed tomography, as well as endocrinological tests to confirm the diagnosis prior to the adrenalectomy. The primary endpoint was improved metabolic parameters relating to diabetes, hypertension, dyslipidemia, and obesity. Results: Patients with SCS compared to those with NFA showed a higher occurrence of diabetes (29.5% vs. 11.4%), hypertension (59.1% vs. 34.3%), and dyslipidemia (43.2% vs. 14.3%). Patients with SCS showed a smaller median tumor size compared to those with NFA (2.5 cm vs. 5 cm). No significant perioperative complications ${\geq}$ Clavien-Dindo classification grade III were observed in any patient (SCS or NFA group). In terms of their metabolic profile, patients with SCS showed a significant postoperative improvement in hypertension (50.0%), diabetes (53.9%), dyslipidemia (31.6%), and obesity (29.2%). However, patients with NFA showed a postoperative improvement only in dyslipidemia (40.0%) and obesity (4.8%). Conclusions: Owing to absence of significant perioperative complications and the marked postoperative improvement in metabolic impairment, LA is a useful treatment strategy in patients diagnosed with SCS. In contrast, LA was not observed to show beneficial effects in correcting/improving the metabolic profile in patients presenting with NFA.

The Effect of Individual Differences in Need for Affective and Cognitive on Health Advice in Virtual Reality (감성 욕구와 인지 욕구의 개인차가 가상현실의 건강 조언에 미치는 영향)

  • Yu, Sanghyeong;Jung, Yujin;Han, Kwanghee
    • Science of Emotion and Sensibility
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    • v.22 no.3
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    • pp.77-90
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    • 2019
  • In this study, we investigated which message provider is effective in a virtual reality (VR) environment for individuals with different needs with regard to affect (need for affect [NFA]) and cognition (need for cognition [NFC]). According to Haddock et al (2008), individuals with high NFA were more influenced to change their behavior by the emotional aspects of a message, whereas individuals with high NFC were more influenced by the cognitive aspects of the same message. We hypothesized that individual differences in needs could affect not only receipt of the message but also the acceptability of the message provider. For example, someone with high NFA might accept messages more easily from an acquaintance than from experts. In the VR environment, the appearance of the message provider could be manipulated in a way that makes him or her more familiar to the person receiving the message. Accordingly, in order to promote the effectiveness of message providers in a VR environment according to the individual difference in needs, we measured the level of the preference and self-efficacy according to needs (NFA or NFC), type of message provider (expert, significant other, or other), and VR device (text or VR). Contrary to what we expected, the results showed that there was no matching effect between the needs and the message provider. However, we found that level of preference and self-efficacy were significantly high when a VR device was worn only by participants with high NFA. This result suggests that a VR environment is more suitable for providing health advice to people with high NFA. In addition, the novelty of this study is that we tried to find the tailored message provider on health advice in VR environment and it is in the early stage of the research.

Generating various NPCs Behavior using Inference of Stochastic Finite Automata (확률 유한오토마타의 추론을 이용한 다양한 NPC의 행동양식 생성에 관한 기법 연구)

  • Cho, Kyung-Eun;Cho, Hyung-Je
    • Journal of Korea Game Society
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    • v.2 no.2
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    • pp.52-59
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    • 2002
  • This paper introduces FSM, statistical FSM and NFA that are used for assigning behaviors of NPCs in computer games. We propose a new method for remedy of the weakness of previous studies. We use the method of inferencing stochastic grammars to generate NPCs behaviors. Using this method we can generate a lot of MPCs or Computer Players behaviors automatically and the games will be more enjoyable.

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Antioxidant and Anti-Obesity Activity of Ethanol Extracts from Fermented Arctium lappa L. (발효 우엉 에탄올 추출물의 항산화 및 항비만 활성)

  • Kim, Mi Hyun;Kim, Jong Gyu;Choi, Jae Hong
    • The Korean Journal of Food And Nutrition
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    • v.28 no.5
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    • pp.752-758
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    • 2015
  • This study was conducted to investigate the antioxidant and anti-obesity activity of ethanol extracts from fermented Arctium lappa L. Arctium lappa fermented twice with Phellinus linteus ($2^{nd}$ FA) showed more DPPH radical scavenging activity than non-fermented Arctium lappa (NFA), and Arctium lappa fermented once ($1^{st}$ FA) at a concentration of 62.5 ppm. The $2^{nd}$ FA showed the highest level of ABTS radical scavenging activity at concentration range of 31.5~125 ppm. The ABTS free radical scavenging activities of $1^{st}$ FA and $2^{nd}$ FA were similar to that of BHA, a synthetic antioxidant, at a concentration of 250 ppm. The total polyphenol content of $2^{nd}$ FA was higher than those of NFA and $1^{st}$ FA. The flavonoid content was significantly increased in $1^{st}$ FA and $2^{nd}$ FA than NFA. During adipocyte differentiation, lipid accumulation in 3T3-L1 cells was significantly decreased in the order of NFA, $1^{st}$ FA, and $2^{nd}$ FA at all concentrations. In conclusion, the antioxidant and anti-obesity activities of A. lappa were increased depending on the degree of fermentation. It is suggested that fermented Arctium lappa, especially $2^{nd}$ FA, could be used as a natural ingredient for functional foods and medicine.

A Novel System with EMG-controlled FES Enhanced Gait Function and Energy Expenditure for Older Adults

  • Jang-hoon Shin;Hye-Kang Park;Joonyoung Jung;Dong-Woo Lee;Hyung cheol Shin;Hwang-Jae Lee;Wan-hee Lee
    • Physical Therapy Rehabilitation Science
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    • v.13 no.2
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    • pp.152-162
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    • 2024
  • Objective: This study was conducted to analyze the effect of wearable Electromyography-controlled functional electrical stimulation (EMG-controlled FES) System on Gait Function and cardiopulmonary metabolic efficiency during walking in older adults. Design: Cross-section study Methods: Total 22 older adult participants suitable to selection criteria of this study participated in this study. The EMG-controlled FES System, which functions as a wearable physical activity assist FES system was used. All participations performed randomly assigned two conditions (Non-FES assist [NFA], FES assist [FA]) of walking. In all conditions, spatio-temporal parameters and kinematics and kinetics parameters during walking was collected via 3D motion capture system and 6 minutes walking test (6MWT) and metabolic cost during walking and stairs climbing was collected via a portable metabolic device (COSMED K5, COSMED Srl, Roma, Italy). Results: In Spatio-temporal parameters aspects, The EMG-controlled FES system significantly improved gait functions measurements of older adults with sarcopenia at walking in comparison to the NFA condition (P<0.05). Hip, knee and ankle joint range of motion increased at walking in FA condition compared to the NFA condition (P<0.05). In the FA condition, moment and ground reaction force was changed like normal gait during walking of older adults in comparison to the NFA condition (P<0.05). The EMG-controlled FES system significantly reduced net cardiopulmonary metabolic energy cost, net energy expenditure measurement at stairs climbing (P<0.05). Conclusions: This study demonstrated that EMG-controlled FES is a potentially useful gait-assist system for improving gait function by making joint range of motion and moment properly.

A Study on the Verification of Network Flow Analysis Methodology of CHECWORKS Program used in Pipe Wall Thinning Management (배관감육관리에 활용되는 CHECWORKS 프로그램의 열수력해석 방법론 검증에 관한 연구)

  • Seo, Hyuk Ki;Hwang, Kyeong Mo
    • Corrosion Science and Technology
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    • v.12 no.2
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    • pp.79-84
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    • 2013
  • In general, pipelines at nuclear power plants are affected by various types of degradation mechanisms and may be ruptured after gradually thinning. FAC (Flow-Accelerated Corrosion) is typical aging mechanism affecting the secondary side piping system. In Korea nuclear power plants, CHECWORKS program have been used for management of wall thinning damages. However, sometimes, CHECWORKS program shows wrong results at the stage of NFA (Network Flow Analysis) in case of complex pipelines. This paper describes the calculation results of pressure drop in a complex pipeline and single line by using the CHECWORKS program and the analysis results are compared with those of engineering calculation results including errors between them.

A Keyword-based Filtering Technique of Document-centric XML using NFA Representation (NFA 표현을 사용한 문서-중심적 XML의 키워드 기반 필터링 기법)

  • Lee Kyoung-Han;Park Seog
    • Proceedings of the Korean Information Science Society Conference
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    • 2006.06c
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    • pp.25-27
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    • 2006
  • XPath 명세는 XML 원소 내용을 필터링하기 위한 질의어 작성이 어렵다. 본 논문은 이러한 문제점을 해결하기 위해 SQL의 LIKE 연산자에서 사용되던 특별한 매칭 문자 '%' 를 허용한 확장된 XPath 명세와 그것을 표준 질의어로 사용하는 문서-중심적 XML 필터링 기법인 Pfilter를 제안한다. Pfilter는 값-기반 술어(value-based predicate)에서 피연산자의 공통 앞부분 문자를 공유하여 값-기반 술어의 처리 성능을 향상시킨다. 또한 본 논문은 Pfilter와 대표적인 데이터-중심적 XML 필터링 기법인 Yfilter를 값-기반 술어 처리의 확장성과 효율성에 대해 비교하고 Pfilter의 값-기반 술어 삽입, 삭제, 처리 결과를 제공한다.

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Stealth, electromagnetic interception, and electrical properties of aluminum sputtered clothing materials - Focusing on the density change - (알루미늄 스퍼터링 처리 의류소재의 스텔스 특성과 전자파 차단 및 전기적 특성에 관한 연구 - 밀도 변화를 중심으로 -)

  • Han, Hye Ree
    • The Research Journal of the Costume Culture
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    • v.30 no.4
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    • pp.579-593
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    • 2022
  • This study examines the surface characteristics, electrical conductivity, electromagnetic wave blocking characteristics, infrared (IR) transmittance, stealth function, thermal characteristics, and moisture characteristics of IR thermal imaging cameras. Nylon film (NFi), nylon fabric (NFa), and 5 types of nylon mesh were selected as the base materials for aluminum sputtering, and aluminum sputtering was performed to study IR thermal imaging, color difference, temperature change, and so on, and the relationship with infrared transmittance was assessed. The electrical conductivity was measured and the aluminum-sputtered nylon film demonstrated 25.6kΩ of surface resistance and high electrical conductivity. In addition, the electromagnetic wave shielding characteristics of the sputtering-treated nylon film samples were noticeably increased as a result of aluminum sputtering treatment as measured by the electromagnetic wave blocking characteristics. When NFi and NFa samples with single-sided sputtering were placed on the human body (sputtering layer faced the outside air) and imaged using IR thermographic cameras, the sputtering layer displayed a color similar to the surroundings, showing a stealth effect. Moreover, the tighter the sample density, the better the stealth function. According to the L, a, b measurements, when the sputtering layer of NFi and NFa samples faced the outside air, the value of a was generally high, thereby demonstrating a concealing effect, and the △E value was also high at 124.2 and 93.9, revealing a significant difference between the treated and untreated samples. This research may be applicable to various fields, such as the military wear, conductive sensors, electromagnetic wave shielding film, and others.