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Development of Analysis Method of Gardenia Yellow as Natural Colorants and Content Survey in Commercial Foods by HPLC (HPLC를 이용한 천연착색료인 치자황색소의 분석법 개발 및 시판 식품중 함유량조사)

  • Kim, Hee-Yun;Kim, So-Hee;Hong, Ki-Hyoung;Lee, Chul-Won;Kim, Kil-Saeng;Ha, Sang-Chul;Jo, Jae-Sun
    • Korean Journal of Food Science and Technology
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    • v.31 no.4
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    • pp.945-951
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    • 1999
  • A simple and practical method for the determination of gardenia yellow in foods was developed. In this method, analysis of gardenia yellow in food products has been carried out by the detection of crocetin and/or geniposide as indicator compounds. As a new analytical method for gardenia yellow, we adopted crocetin, which is produced from colored components of gardenia yellow by alkaline hydrolysis, as an indicator compound. The analysis of gardenia yellow was performed by reverse phase high performance liquid chromatography using a Capcell pak $C_{18}$ column at wave length 240 nm (geniposide) and 435 nm (crocetin). The recovery rates of geniposide and crocetin were found to be 93.4% and 87.8% for Dan Mu Ji, 90.2% and 85.9% for milk, 92.8% and 86.5% for snack, respectively. With this method, the range of crocetin and geniposide contents $({\mu}g/g)$ were as follows: $ND{\sim}1.7$ and $ND{\sim}14.1$ for Dan Mu Ji, $ND{\sim}0.2$ and $ND{\sim}13.6$ for milk, $ND{\sim}1.6$ and $ND{\sim}0.9$ for snack, respectively. The detection limits of crocetin and geniposide were 0.07 ${\mu}g/g$ and 0.05 ${\mu}g/g$, respectively.

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Effects of Xingjian(LR2),Shaofu(HT8) by Reinforcing and Reducing on the Regional Cerebral Blood Flow and Mean Arterial Blood Pressure in Normal Rats (행간(行間)·소부(少府) 보사침법(補瀉鍼法)이 국소뇌혈류량(局所腦血流量) 및 평균혈압(平均血壓)에 미치는 영향(影響))

  • Shin, Jeong-cheol;Ryu, Chung-ryul;Cho, Myeng-rae
    • Journal of Acupuncture Research
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    • v.20 no.6
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    • pp.190-200
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    • 2003
  • Objective: The purpose of this study is to examine influences by the order, Reduction and Reinforcement in Acupuncture on cerebral hemodynamics [regional cerebral blood flow(rCBF) and mean arterial blood pressure(MABP)] in normal rats. Methods: This experiments was to to investigate eath other changes of rCBF and MABP at Xingjian(LR2)(1st) Shaofu(HT8)(2nd) Reduction, Xingjian(LR2)(1st) Shaofu(HT8)(2nd) Reinforcement, Shaofu(HT8)(1st) Xingjian(LR2)(2st) Reduction and Shaofu(HT8)(1st) Xingjian(LR2)(2st) Reduction in Acupuncture. Results: 1. LR2(1st) HT8(2nd) Reduction in Acupuncture was decreased rCBF and MABP in compared with normal condition. 2. LR2(1st) HT8(2nd) Reinforcement in Acupuncture was significantly decreased rCBF, and was decreased MABP in compared with normal condition. 3. HT8(1st) LR2(2st) Reduction in Acupuncture was decreased rCBF during acupuncture but was recovered rCBF after with-drawing of the neddle. 4. HT8(1st) LR2(2st) Reduction in Acupuncture was decreased MABP during acupuncture and after withdrawing of the neddle. 5. HT8(1st) LR2(2st) Reinforcement in Acupuncture was significantly increased rCBF during acupuncture and 30min after withdrawing of the neddle. 6. HT8(1st) LR2(2st) Reinforcement in Acupuncture was decreased MABP during acupuncture, but was recovered MABP after withdrawing of the neddle. Conclusions: I suggested that LR2 HT8 Reduction in Acupuncture and LR2 HT8 Reinforcement in Acupuncture cause a diverse response of cerebral hemodynas.

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A study of the determination of off-set position for Nd:YAC laser welding between SCP steel sheet and STS304 sheet (Nd:YAG 레이저빔을 이용한 SCP 강판과 STS304강판 용접시 오프셋(off-set) 위치 결정에 관한 연구)

  • Yoon B. S.;Kim T. H.;Park G. Y.;Lee G. D.
    • Laser Solutions
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    • v.7 no.2
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    • pp.1-10
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    • 2004
  • This work was attempted to join SCP sheet and STS304 sheet by using Nd:YAC laser beam. SCP sheet has good formability and low cost, while STS304 has excellent corrosion resistance and mechanical properties in high temp. In this experiment, butt joint type was used to develop the tailored blank welding for dissimilar steel. Sheets which have different thermal properties. Computer simulation was conducted to obtain the off-set position for efficient welding by considering laser power, scanning speed, focal length and basic properties. The result showed that the optimum thermal distribution was obtained when the laser beam was irradiated at $0.05{\sim}0.1$ mm off-set toward the SCP sheet side. The experiment was conducted based on the result of computer simulation to show the same optimum conditions. Optimum conditions were 3KW in laser beam power, 6m/min in scanning speed, -0.5mm in focal position, 0.1mm off-set toward SCP. Microhardness test, tensile test, bulge test, optical microscopy, EDS, and XRD were performed to observe the microstructure around fusion zone and to evaluate the mechanical properties of optimum conditions, The weld zone had high microhardness values by the formation of the martensitic structure. Tensile test measured the strength of welded region by vertical to strain direction and the elongation of welded region by parallel to strain direction. Bulge test showed $52\%$ formability of the original materials. Bead shape, grain size, and martensitic structure were observed by the optical microscopy in the weld zone. Detailed results of EDS, XRD confirmed that the welded region was connected of martensitic structure.

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A Study on the improvement of treatment efficiency in a conventional sewage treatment plant (기존 하수처리장에서의 처리 효율개선에 관한 연구)

  • 안철우;박진식;문추연
    • Journal of environmental and Sanitary engineering
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    • v.15 no.3
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    • pp.50-56
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    • 2000
  • In this study, sewage were treated with operating Two-step Aeration System and conventional activated sludge process together in a condition. At the same HRT 8hr of Two-step Aeration System and Activated Sludge Process, BOD treatment efficiency of 1st sedimentation basin effluent 36.9% by Two-step Aeration system was 12.3% higher than 24.65 by Activated Sludge Process and the COD treatment efficiency 39.8% by two-step Aeration System was 11.6.3% higher than 28.2% by Activated Sludge Process. BOD and COD treatment efficiencies of 2nd sedimentation basin effluent were 88.1% and 85.6% Two-step Aeration System and were 83.8% and 82.3% Activated Sludge Process. In the first treatment, as BOD was relatively removed a lot, F/M ratio 0.17, $0.21{\cdot}BOD/kg{\cdot}MLSS.d$ was maintained by Activated Sludge Process. Therefore it was proved that organic matter treatment efficiency by Two-step Aeration System os Higher than by Activated Sludge Process in a aeration time 8hr. $NH_4^{+}-N$ treatment efficiencies were 55.5% by Two-step Aeration System and 39.75 by Activated sludge Process. $NO_3^{-}-N$ concentration in 2nd. sedimentation basin effluent were 3.33% by Two-step Aeration System and 2.36% by Activated Sludge Process. From this result, Two-step Aeration System was proved more advantageous treatment process for nitrification than Activated Sludge Process. The fluctuation range of BOD, COD and SS concentration in 2nd sedimentation basin effluent $16~33mg/{\ell}$, $15~23mg/{\ell}$ and $14~22mg/{\ell}$ by Two-step Aeration System was smaller than $16~57mg/{\ell}$, $15~25mg/{\ell}$ by Activated sludge Process. Overall the fluctuation range in 2nd sediment basin effluent by was smaller than by Activated Sludge Process. As a result, it is possible for this Two-step Aeration with no facility investment and a little of operation condition change in a conventional sewage treatment plant to get stability and nitrification of treatment water quality.

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Treatment of Partial Thickness Burn Wounds with Cultured Epidermal Homografts (동종 배양표피를 이용한 부분층 2도 화상의 치료)

  • Choi, Joong Ho;Ko, Jang Hyu;Seo, Dong Kook;Lee, Jong Wook;Jeon, Saewha;Oh, Suk Joon;Jang, Young Chul
    • Archives of Plastic Surgery
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    • v.33 no.5
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    • pp.587-591
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    • 2006
  • Purpose: Since Rheinwald and Green laid the foundation of epidermal cell culture technology in 1975, many clinicians and scientists have attempted to prove the effectiveness of cultured epidermal autologous(CEA) or homogenetic(CEH) grafts in the wound healing process. In contrast to CEA which cultured from a patient's skin on demand, Cultured Epidermal Homograft(CEH) can be readily available to use on cleaned wounds. In this study, we conducted a controlled clinical trial in order to confirm the effectiveness of CEH in treating partial-thickness 2nd degree burn wounds. Methods: From July 2003 to January 2004 at Hangang Sacred Heart Hospital, we performed a clinical trial in which 35 patients who suffered from 2nd degree burns were enrolled. Wounds were randomly divided into two parts, control and test sites. Test sites were treated with allogeneic keratinocyte sheets ($Kaloderm^{(R)}$, Tegoscience Inc.), a CEH commercialized in Korea. Results: All wounds healed completely without any major complication. The complete healing took $8.3{\pm}2.8$($mean{\pm}S.D.$) days in the test sites as opposed to $11.7{\pm}3.3days$ in the control sites. Conclusion: Based on these results, we concluded that CEH accelerates re-epithelialization of partial thickness burn wounds and CEH can be an safe alternative to skin grafts for 2nd degree burns.

Effect of Welding Speed on Mechanical Properties and Formability in Nd:YAG Laser Welds of 1000MPa Grade DP Steel (1000MPa급 DP강의 Nd:YAG 레이저 용접부의 기계적 성질과 성형성에 미치는 용접 속도의 영향)

  • Jang, Jin-Young;Choi, Woo-Nam;Jung, Byung-Hun;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.27 no.2
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    • pp.69-75
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    • 2009
  • The effects of welding speed were investigated on penetration characteristics, defects and mechanical properties including formability test in Nd:YAG laser welded 1000MPa grade DP steels. A shielding gas was not used and bead-on-plate welding was performed with various welding speeds at 3.5kW laser power. Defects of surface and inner beads were not observed in all welding speeds. As the welding speed increased, the weld cross-section varied from the trapezoid having wider bottom bead, through X type, finally to V type in partial penetration range of welding speeds. The characteristic of hardness distribution was also investigated. The center of HAZ had maximum hardness, followed by a slight decrease of hardness as approaching to FZ. Significant softening occurred at the HAZ near BM. Regardless of the welding speed, the weld showed approximately the same hardness distribution. In the perpendicular tensile test with respect to the weld direction, all specimens were fractured at the softening zone. In the parallel tensile test to the weld direction, the first crack occurred at weld center and then propagated into the weld. Good formability over 80% was taken for all welding conditions.

Tropospheric Data of KASI GNSS Network (2001-2014) Based on the CODE's 2nd Reprocessing Product

  • Roh, Kyoung-Min;Park, Han-Earl;Choi, Byung-Kyu
    • Journal of Positioning, Navigation, and Timing
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    • v.9 no.3
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    • pp.229-236
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    • 2020
  • The trend of water vapor contents in atmosphere is one of key elements for studying climate change. The tropospheric products, i.e., ZTD values achieved through GPS data processing can retrieve the amount of water vapor with higher temporal and spatial resolution than any other instruments. In this study, the tropospheric products of KASINET for a time period from 2001 to 2014 are reprocessed using PPP strategy and the products from the CODE's 2nd reprocessing campaign. For consistency with reprocessing activities of other networks like EPN, the VMF1 mapping function and non-tidal loading effect due to atmospheric pressure are applied in the process. The reprocessing results are investigated through comparing with the CODE's 2nd reprocessing products by including some IGS stations in the process and also calculating weekly coordinate repeatability to see the quality of the processing. After removing outliers based on the variation of averaged formal error, all processed stations have similar variations of formal error about 2 mm which is lower than that of the IGS final product. Comparison results with the CODE's 2nd reprocessing products show that the overall mean difference is found to be -0.28±5.54 mm which is similar level of the previous studies. Finally, the ZTD trends of all KASINET stations are calculated and the averaged trend is achieved as 0.19 mm/yr. However, the trend of each month shows different amounts and directions from -1.26 mm/yr in May to 1.18 mm/yr in August. In conclusion, the reprocessed tropospheric product and applied strategy of this study has enough quality as one of reliable solution for a reference product for Korean Peninsula which is needed to use GPSbased tropospheric product for climate change research.

The Massive Blooms of Gonyaulax polygramma (Gonyaulacales, Dinophyceae) in the Southern Coastal Areas of Korea in Summer, 2009 (2009년 남해안 전역에 발생된 고니아룩스 적조에 관한 연구)

  • Cho, Eun-Seob
    • Journal of Environmental Science International
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    • v.20 no.12
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    • pp.1521-1531
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    • 2011
  • This study was carried out to determine the characteristics of the marine environment, including nutritional content, in order to clearly understand the outbreaks of Gonyaulax polygramma in the southern coastal areas in August, 2009. Samples were collected at 13 sites and water temperature and salinity were measured using a CTD. Field surveys were twice: the first between August 5-7, the second between August 22-24. The cell density of G. polygramma was 6,500-10,000 cells $ml^{-1}$ during the $1^{st}$ survey, whereas during the $2^{nd}$ survey the range of the cells was recorded from 8,000 to 12,500 cells $ml^{-1}$. Cochlodinium polykrikoides ranged from 0 to 105 cells $l^{-1}$ during the field survey. In water environments, the majority stations during the $1^{st}$ survey showed a nearly homogeneous water column below $1^{\circ}C$ in temperature, as well as similar profiles of salinity. However, the stratification between the surface and bottom was observed in the $2^{nd}$ survey. Regarding nutrients, Dissolved Inorganic Nitrogen (DIN) in the surface ranged from 0.144 to 0.236 mg $l^{-1}$ during the $1^{st}$ survey, and 0.082-0.228 mg $l^{-1}$ during the $2^{nd}$ survey. DIP (Dissolved Inorganic Phosphorus) did not show any difference in concentration between the $1^{st}$ and $2^{nd}$ survey. During August of 2009, the wind speed in the southern waters remained at around ${\leq}2\;m\;s^{-1}$ for about 60% of time, and there was very little precipitation during the month. Irradiance of ${\geq}10$ hr was shown in the late of August. It is thought that a low level of DIN and salinity play an important role as an essential factor for rapid growth, wide distribution and longer duration of red tide in G. polygramma.

Effect of the welding speed on the characteristics of Nd:YAG laser welds for automotive application : 600MPa PH high strength steel (600MPa급 자동차용 석출경화형 고장력강판 Nd:YAG 레이저 용접부의 특성에 미치는 용접속도의 영향)

  • Han, Tae-Kyo;Jung, Byung-Hun;Kang, Chung-Yun
    • Laser Solutions
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    • v.10 no.3
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    • pp.25-32
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    • 2007
  • The effect of welding speed on the weldability, microstructures, hardness, tensile property of Nd:YAG laser welding joint in 600MPa grade precipitation hardening high strength steel was investigated. A shielding gas was not used, and bead-on-plate welding was performed using various welding speeds at a power of 3.5kW. Porosity in the joints occurred at 1.8m/min, but were not observed over the welding speed of 2.1m/min. However, spatter occurred over the welding speed of 6.6m/min. The hardness was the highest at heat affected zone(HAZ) near fusion zone(FZ), and was decreased on approaching to the base metal. The maximum hardness increased with increasing welding speed. The microstructure of FZ was composed of coarse grain boundary ferrite and bainite(upper) but the HAZ near the FZ contained bainite(Lower) and fine ferrite at a low welding speed. With increasing welding speed, ferrite at the FZ and the HAZ became finely and upper binite changed to lower bainite. In a perpendicular tensile test to the weld line, all specimens were fractured at the base metal, and the tensile strength and the yield strength of joints was equal to those of raw material. Elongation was found to be lower than that of the raw material.

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Investigation of Cutting Characteristics in the Sharp Edge for the Case of Cutting of a Low Carbon Steel Sheet using High-power CW Nd:YAG Laser (고출력 CW Nd:YAG 레이저를 이용한 저탄소 냉연강판 절단시 모서리부 절단 특성 분석)

  • Ahn, Dong-Gyu;Yoo, Young-Tae
    • Journal of Welding and Joining
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    • v.24 no.4
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    • pp.32-38
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    • 2006
  • The objective of present research works is to investigate the effects of process parameters, including the power of laser, cutting speed, material thickness, and the edge angle, on the melted area in the sharp edge of the cut material fur the case of cutting of a low carbon steel sheet using high-power CW Nd:YAG laser. In order to investigate the influence of edge angle and size of loop on the melted area in the sharp edge, angular cutting tests and loop cutting tests have been carried out. From the results of angular cutting tests, the relationship between the edge angle and the melted area has been obtained. The results of the experiments have been shown that the melted area is rapidly reduced from $120^{\circ}$ of the edge angle and the melted area is nearly zero at $150^{\circ}$ of the edge angle. Through the results of loop cutting experiments, the relationship between the cutting angle on the melted area in the edge according to the size of loop have been obtained. In addition, it has been shown that a proper size of loop is nearly 3 mm as the corner angle is greater than $90^{\circ}$ and 5 mm as the comer angle is less than $90^{\circ}$. The results of above experiments will be reflected on the knowledge base to generate optimal cutting path of the laser.