• 해양환경안전학회지
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• 제24권4호
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• pp.482-488
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• 2018

선박 축계는 프로펠러 하중의 영향으로 선미관 후부베어링의 국부하중 증가가 현저히 나타나 축계 선미관 베어링 손상의 위험이 증대된다. 이를 방지하기 위해 수행된 추진축계 정렬연구는 주로 준정적 상태(quasi-static condition)에서 축과 지지베어링간의 상대적 경사각을 감소시키는데 중점을 두어 진행되어 왔다. 그러나 보다 상세한 평가를 위해서는 동적상태를 추가로 고려하는 것이 필요하다. 4,700 DWT 선박을 대상으로 NCR로 운전중 급속으로 우현 전타할 때 추진축계가 받는 영향에 대해 연구하였다. 연구결과 선미 유동장 변화에 의해 프로펠러 편심추력이 과도 상태가 되어 프로펠러에 불평형 진동이 유발되는 것을 확인하였다. 우현 전타시의 프로펠러 편심추력은 NCR 조건대비 축을 선미관 베어링으로부터 들어 올리는 힘으로 작용하여 선미관 베어링 하중완화에 기여하고 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제31권5호
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• pp.659-666
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• 2021

After Candida albicans, Candida glabrata is one of the most common fungal species associated with candidemia in nosocomial infections. Rapid acquisition of nutrients from the host is important for the survival of pathogens which possess the metabolic flexibility to assimilate different carbon and nitrogen compounds. In Saccharomyces cerevisiae, nitrogen assimilation is controlled through a mechanism known as Nitrogen Catabolite Repression (NCR). NCR is coordinated by the action of four GATA factors; two positive regulators, Gat1 and Gln3, and two negative regulators, Gzf3 and Dal80. A mechanism in C. glabrata similar to NCR in S. cerevisiae has not been broadly studied. We previously showed that in C. glabrata, Gln3, and not Gat1, has a major role in nitrogen assimilation as opposed to what has been observed in S. cerevisiae in which both factors regulate NCR-sensitive genes. Here, we expand the knowledge about the role of Gln3 from C. glabrata through the transcriptional analysis of BG14 and gln3Δ strains. Approximately, 53.5% of the detected genes were differentially expressed (DEG). From these DEG, amino acid metabolism and ABC transporters were two of the most enriched KEGG categories in our analysis (Up-DEG and Down-DEG, respectively). Furthermore, a positive role of Gln3 in AAA assimilation was described, as was its role in the transcriptional regulation of ARO8. Finally, an unexpected negative role of Gln3 in the gene regulation of ABC transporters CDR1 and CDR2 and its associated transcriptional regulator PDR1 was found. This observation was confirmed by a decreased susceptibility of the gln3Δ strain to fluconazole.

• The Plant Pathology Journal
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• 제17권1호
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• pp.22-28
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• 2001

Barley yellow dwarf virus PAV (BYDV-PAV) has a 5.7-kb positive-sense single-stranded RNA genome that contains six open reading frames (ORFs). BYDV-PAV produces three subgenomic RNAs (sgRNAs). The largest of which encodes the coat, 17-kDa, and readthrough proteins from two initiation codons. To investigate the role of intergenic and 3'-proximal noncoding regions (NCRs) in coat protein (CP) expression and BYDV-PAV replication, a full-length infectious cDNA of the RNA genome of an Illinois isolate of BYDV-PAV was constructed downstream of the Cauliflower mosaic virus-35S promoter. Linear DNA molecules of these cDNAs were infectious, expressed the 22-kDa CP, and produced both genomic RNA sgRNAs in ratios similar to those observed in protoplasts inoculated with viral RNA. The portion of 5'NCR of sgRNA1 between ORFs 2 and 3 was not required for, but enhanced translation of CP from ORF3. Mutants containing deletions in the NCR downstream of ORF5 failed to replicate in oat protoplasts. These results indicate that an intact 3$^1$NCR is required for BYDV-PAV replication.

• IMMUNE NETWORK
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• 제21권4호
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• pp.25.1-25.16
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• 2021

Asthma is a heterogeneous disease whose development is shaped by a variety of environmental and genetic factors. While several recent studies suggest that microbial dysbiosis in the gut may promote asthma, little is known about the relationship between the recently discovered lung microbiome and asthma. Innate lymphoid cells (ILCs) have also been shown recently to participate in asthma. To investigate the relationship between the lung microbiome, ILCs, and asthma, we recruited 23 healthy controls (HC), 42 patients with non-severe asthma, and 32 patients with severe asthma. Flow cytometry analysis showed severe asthma associated with fewer natural cytotoxicity receptor (NCR)⁺ILC3s in the lung. Similar changes in other ILC subsets, macrophages, and monocytes were not observed. The asthma patients did not differ from the HC in terms of the alpha and beta-diversity of the lung and gut microbiomes. However, lung function correlated positively with both NCR⁺ILC3 frequencies and microbial diversity in the lung. Sputum NCR⁺ILC3 frequencies correlated positively with lung microbiome diversity in the HC, but this relationship was inversed in severe asthma. Together, these data suggest that airway NCR⁺ILC3s may contribute to a healthy commensal diversity and normal lung function.

• 대한바이러스학회지
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• 제28권4호
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• pp.295-302
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• 1998

The RNA genome of poliovirus encodes a long polyprotein precursor and this polyprotein is cleaved proteolytically by viral protease to yield mature proteins. The mature proteins derived from the P1 polyprotein precursor are the component of capsids. To further delineate the process of capsid assembly and encapsidation, in a first attempt, a cell line which expresses the authentic P1 polyprotein was established. CV-1 cells were transfected with the pRCRSVS1P1 plasmid DNA which contains 5'ncr sequences, whole authentic capsid gene of poliovirus and neomycin resistance gene. These cells were treated with G418 for 3 months, and eventually G418 resistant cells were selected and formed colonies. Each colony was picked and grown in the media containing G418. DNA analysis indicated that 1 of 13 neomycin resistant cell lines (R2-18) contains whole poliovirus P1 capsid gene segment which was incorporated into the genome. Immuneprecipitation of cell lysates with sera from rabbit immunized with inactivateded Sabin type 1 particles demonstrated the constitutive expression of the poliovirus P1 capsid protein from R2-18.

• 한국도서관정보학회지
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• 제32권2호
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• pp.213-237
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• 2001

전자자료의 조직에 기초가 되는 목록을 위해서는 서지기술의 준거가 되는 목록규칙과 자동화목록을 위한 MARC형식의 개발이 필수적이다. 이 연구는 전자자료의 특성 및 종류를 조사하고, AACR2R, NCR1994, ISBD(ER), KORMARC기술규칙 등의 목록규칙과 MARC21 ,OCLC-MARC, KORMARC 등의 MARC형식의 분석을 바탕으로 전자자료의 목록을 위한 KCR과 KORMARC의 개선 방안을 제시하였다.

• Asian Journal of Business Environment
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• 제6권1호
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• pp.25-30
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• 2016

Purpose - The study aims to examine the students' perspective (stream wise) of parameters affecting the undergraduate engineering education system present in a private technical institution in NCR, Haryana, India. Research design, data, and methodology - It is a descriptive type of research in nature. Questionnaire Based Survey has been used to collect the data. The sample size for the study is 500 comprising of the students respondents. The sample has been taken randomly and the questionnaire was filled by the students (pursuing B. Tech) chosen on the random basis from a private technical educational institution in NCR, Haryana, India. For data analysis and conclusion of the results of the survey, statistical tool like F test was performed with the help of high quality software; SPSS. Conclusion - Analysis of variance revealed statistically no difference between the mean number of the groups (stream wise) for the parameters "Selection", "Academic Excellence", "Infrastructure", "Personality Development and Industry Exposure" and "Management and Administration". While Analysis of variance revealed statistically difference between the mean numbers of the groups for the parameter "Placements".

• 한국전자거래학회:학술대회논문집
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• 한국전자거래학회 2001년도 International Conference CALS/EC KOREA
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• pp.543-557
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• 2001

- 시장의 경쟁환경 변화와 고객니즈의 다양화 --> 현업사용자의 다양한 정보 가공 및 분석 요구 - 분석적이고 과학적인 고객 맞춤형 마케팅 필요(중략)

• 한국정보기술응용학회:학술대회논문집
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• 한국정보기술응용학회 2001년도 추계공동학술대회 논문자료집 정보화 젼략 패러다임의 변화에 대한 보기술의 대응
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• pp.79-80
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• 2001

• The Plant Pathology Journal
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• 제20권3호
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• pp.212-219
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• 2004

Three isolates of Cucumber mosaic virus (CMV) from lily plants showing mosaic and distortion symptoms were detected by reverse-transcriptase polymerase chain reaction (RT-PCR) using primers specific to Cucumovirus genus namely, LK-CMV, LK4-CMV, and LKS-CMV. Restriction enzymes patterns of the RT-PCR products revealed that the lily isolates belonged to subgroup IA of CMV. In terms of biological properties, the lily isolates have highly similar but distinct pathogenicity as reported in other lily strains and ordinary strains of CMV. To characterize the molecular properties, cDNAs containing coat protein (CP) gene and 3' non-coding region (NCR) of RNA3 for the isolates were cloned and their nucleotide sequences were determined. The CP similarity (218 amino acids) was highly homologous (>97％) with that of subgroup I CMV strains. However, an additional 20-nulcleotide long segment was only present in 3' NCR of lily isolates, which form an additional stem-loop RNA structure. By using chimeric construct exchange cDNA containing 3'NCR of LK-CMV into the full-length cDNA clone of RNA3 of Fny-CMV, this additional segment may prove to be significant in the identification and fitness of the virus in lily plants. The pathology of zucchini squash infected by F1F2L3-CMV, a pseudorecombinant virus was showed to change drastically the severe mosaic and stunting symptom into a mild chlorotic spot on systemic leave, compared with Fny-CMV. To delimit the sequence of RNA3 affected the pathology, various RNA3 chimeras were constructed between two strains of CMV. The symptom determinants of F1F2L3-CMV were mapped to the positions amino acid 234, 239, and 250 in 3a movement protein (MP). RNA3 chimeras changed the sequences encoding three amino acids were resulted in alteration of systemic symptom.