• Journal of Life Science
• /
• v.34 no.3
• /
• pp.170-178
• /
• 2024

Adipose-derived stem cells (ADSCs) are capable of differentiation into multiple lineages of cells, which has attracted attention for clinical therapy. However, ADSCs have poor proliferation capacity and a short life span in culture, which is an impediment in the application to clinical use. Previously, to overcome growth disadvantages, we had established an immortalized ADSC line (ADSC-T) by introducing the SV40 T antigen coding gene into primary human ADSC. In the present study, we evaluated the differentiation potential of this cell line and assessed the anti-inflammatory effect of its conditioned medium (CM). ADSC-T appeared to maintain the differentiation potential into adipocyte and chondrocyte. The CM of ADSC-T suppressed the NF-κB activity and its target gene expression of COX-2 and iNOS. Furthermore, the phosphorylations of MAPKs, including ERK, JNK and p38, were suppressed by the ADSC-T CM. The expressions of pro-inflammatory cytokines such as TGF-β, TNF-α, IL-6, and IL-13 were also suppressed by the CM of ADSC-T. In the Nc/Nga atopic model mice, the CM showed therapeutic effect on DNCB-induced atopic dermatitis. These results indicate that the immortalized ADSC-T maintains the beneficial properties of primary ADSC and could be a versatile cell source for not only research into ADSC but also for production of CM suitable for clinical application.

• Korean Journal of Poultry Science
• /
• v.42 no.1
• /
• pp.15-26
• /
• 2015

In this study, genotyping was executed by using 27 microsatellite markers for genetic diversity of 469 Korean Native Chickens [20 population, each population is 24 samples but Hanhyup A line is 13 samples). in total 469 samples were collected from National Institute of Animal Science (Korean Native Chicken (NR, NY, NG, NL and NW), Ogye (NO), Leghorn F,K (NF and NK), Black and Brown cormish (NH and NS), Rhode Island Red C, D (NC and ND), Total is 12 populations] and Hanhyup [H line (HH), F line (HF), G line (HG), V line (HV), S line (HS), W line (HW), Y line (HY), A line (HA), total is 8 populations]. [The allele number were observed 5 (ADL0268) to 20 (MCW0127) each markers. Observed heterozygostiy ($H_{obs}$), expected heterozygosity ($H_{exp}$), polymorphism Information Content (PIC) were observed 0.359 to 0.677, 0.668 to 0.881 and 0.646 to 0.869, respectively. Using these markers, the calculated the heterozygote deficit within chicken line ($F_{is}$) value each population from mean 0.117. Phylogenetic tree showing the genetic relationship among 20 population using standard genetic distance calculated from 27 microsatellite markers. genetic distances revealed the closest (0.175) between NC and ND. on the other hand, Farthest genetic distances (0.710) revealed between NF and HV. STRUCTURE analysis and Principal Components Analysis (PCA) showed that results of similar phylogenetic tree. The expected probability of identity values on random individuals (Total population and only Hanhyup line) was estimated at $8.80{\times}10^{-83}$ and $3.87{\times}10^{-117}$, respectively. In conclusion, This study shows the useful data that be utilized as a basic data of Korean Native Chicken breeding and development for commercial chicken industry to meet the consumer's demand.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.43 no.1
• /
• pp.40-53
• /
• 2015

To train the manpower to meet the requirements of the industrial field, the introduction of the National Qualification Frameworks(hereinafter referred to as NQF) was determined in 2001 by National Competency Standards(hereinafter referred to as NCS) centrally of the Office for Government Policy Coordination. Also, for landscape architecture in the construction field, the "NCS -Landscape Architecture" pilot was developed in 2008 to be test operated for 3 years starting in 2009. Especially, as the 'realization of a competence-based society, not by educational background' was adopted as one of the major government projects in the Park Geun-Hye government(inaugurated in 2013) the NCS system was constructed on a nationwide scale as a detailed method for practicing this. However, in the case of the NCS developed by the nation, the ideal job performing abilities are specified, therefore there are weaknesses of not being able to reflect the actual operational problem differences in the student level between universities, problems of securing equipment and professors, and problems in the number of current curricula. For soft landing to practical curriculum, the process of clearly analyzing the gap between the current curriculum and the NCS must be preceded. Gap analysis is the initial stage methodology to reorganize the existing curriculum into NCS based curriculum, and based on the ability unit elements and performance standards for each NCS ability unit, the discrepancy between the existing curriculum within the department or the level of coincidence used a Likert scale of 1 to 5 to fill in and analyze. Thus, the universities wishing to operate NCS in the future measuring the level of coincidence and the gap between the current university curriculum and NCS can secure the basic tool to verify the applicability of NCS and the effectiveness of further development and operation. The advantages of reorganizing the curriculum through gap analysis are, first, that the government financial support project can be connected to provide quantitative index of the NCS adoption rate for each qualitative department, and, second, an objective standard is provided on the insufficiency or sufficiency when reorganizing to NCS based curriculum. In other words, when introducing in the subdivisions of the relevant NCS, the insufficient ability units and the ability unit elements can be extracted, and the supplementary matters for each ability unit element per existing subject can be extracted at the same time. There is an advantage providing directions for detailed class program and basic subject opening. The Ministry of Education and the Ministry of Employment and Labor must gather people from the industry to actively develop and supply the NCS standard a practical level to systematically reflect the requirements of the industrial field the educational training and qualification, and the universities wishing to apply NCS must reorganize the curriculum connecting work and qualification based on NCS. To enable this, the universities must consider the relevant industrial prospect and the relation between the faculty resources within the university and the local industry to clearly select the NCS subdivision to be applied. Afterwards, gap analysis must be used for the NCS based curriculum reorganization to establish the direction of the reorganization more objectively and rationally in order to participate in the process evaluation type qualification system efficiently.

• Journal of Korean Academy of Nursing
• /
• v.50 no.3
• /
• pp.487-500
• /
• 2020

Purpose: This study aimed to evaluate the validity and reliability of the Korean version of Menopause-Specific Quality of Life (MENQOL). Methods: The MENQOL was translated into Korean according to algorithm of linguistic validation process. A total of 308 menopausal women were recruited and assessed using the Korean version of MENQOL (MENQOL-K), the World Health Organization Quality of Life Brief Version (WHOQOL-BREF), and Center for Epidemiological Studies Depression Scale (CES-D-K). In estimating reliability, internal consistency reliability coefficients were calculated. Validity was evaluated through criterion validity and construct validity with confirmatory factor analyses using SPSS 23.0 and AMOS 25.0 software. Results: In item analyses, the "increased facial hair" symptom was excluded because of the low contribution of MENQOL-K. The confirmatory factor analysis supported good fit and reliable scores for MENQOL-K model, and the four-factor structure was validated (χ²=553.28, p<.001, NC=1.84, RMSEA=.05, AGIF=.85, AIC=765.28). The MENQOL-K consists of 28 items in 4 domains, including vasomotor (3 items), psychosocial (7 items), physical (15 items), and sexual subscales (3 items). There was an acceptable criterion validity with moderately significant correlation between MENQOL-K and WHOQOL-BREF. The Cronbach's α for the 4 subsacles ranged from .80 to .93. Conclusion: The MENQOL-K is a valid and reliable scale to measure condition-specific quality of life for perimenopausal and postmenopausal women. It can be used to assess the impact of menopausal symptoms on the quality of life of Korean women in clinical trials.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.9 no.6
• /
• pp.602-611
• /
• 2004

Servo system has commonly adapted PI controller with fixed gains, because of its simplicity and determinative relationship among the parameters. The fixed gains PI system may be applied well to some operation conditions, but not non-linearities, complex and time variant operation conditions. For solving these problems, another conventional method, 'variable gun schedule according to speed', is published. The value of gain is determined according to the absolute value of the mover real speed. In this paper, FSTPIC(Fuzzy Self-Tuning PI Controller) is proposed based on various experiences to rapidly reduce speed error and to secure a good speed response characteristics. The effectiveness of proposed algorithms is demonstrated by comparing to two conventional gain systems via 4-quadrant operation.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.7 no.2
• /
• pp.141-150
• /
• 2012

This paper aims at proposing the establishment of concept for qualified startup business and the model of smart incubating business system for qualified startup business creation, which is being government's recent policy paradigm. The result of this paper shows the theoretical setting for the concept of qualified startup business through researching papers precedent and analyzing the startup business education affairs of American universities and proposing the model of requisition for qualified startup business. The core model of requisition for qualified startup business is to secure the essential factors for qualified startup business and the preparation process through compounding the requisites, the execution ecosystem for leading the mixing requisites and supporting. This model has been practiced enormously at business creation education field in American universities. This paper especially propose TEC program of NC State University in U.S.A. by case study.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.37 no.6
• /
• pp.783-790
• /
• 2013

Circular path generation for ball bar measurement using the simultaneous movement of two axes with at least one rotary axis requires the execution of CAM software. However, a change in the machine type or measurement condition requires a new execution of the CAM software, which is cumbersome. This paper presents a circular path generation technique that does not require CAM software and is applicable to different types of driving axes with an arbitrary structural configuration of machine tools and any ball bar setup condition. Mathematical equations are derived for three cases using the proposed technique. In addition, to inspect the measurement feasibility for avoiding physical interference among the ball bar parts, a tilting angle calculation is proposed. The validity of the proposed technique was verified by performing a ball bar experiment with A and C as the simultaneous axes of a five-axis machine tool.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.17 no.1
• /
• pp.37-45
• /
• 2013

In this study, the resistance of various reinforced concrete (RC) slabs subjected to contact detonation was assessed. In order to enhance the blast resistance, fibers and external FRP sheets were reinforced to RC slabs. In the experiment, the $2,000{\times}1,000{\times}100mm$ sized RC slabs were fabricated using normal concrete (NC), steel fiber reinforced concrete (SFRC), polyvinyl alcohol fiber reinforced cementitious composite (PVA FRCC), and ultra-high performance cementitious composites (UHPCC). The damage levels of RC slabs subjected to contact detonation were evaluated by measuring the diameter and depth of crater, spall and breach. The experimental results were compared to the analyzed data using LS-DYNA program and three different prediction equations. The diameter and depth of crater, spall and breach were able to be predicted using LS-DYNA program approximately. The damage process of RC slabs under blast load was also well expressed. Three prediction equations suggested by other researchers had limitations to apply in terms of empirical approaches, therefore it needs further research to set more analytical considerations.

• Magazine of the Korea Concrete Institute
• /
• v.9 no.2
• /
• pp.133-144
• /
• 1997

Concrete plasticity models fol the analysis of reinforced concrete members in plane stress are studied. The proposed plasticity model for reinforced concrete provides a unified approach combining plasticity theory and damage models. It addresses strength mhancement under rnultiaxial compression. and tensile cracking damage. The model uses multiple failure criteria for compressive crushing and tensile cracking. For tensile cracking behavior. rotating-crack and fixed-crack plasticity models are compared. As crushing failure criterion, the Drucker-Prager and the von Mises models are used for comparison. The model uses now and existing damnge models fbr tension softening, tension stiffening. and compression softening dup to tensilt. cracking. Finite element analyses using the unified method are compatxd with existing rxpcrimcntal r.esults. To vei.ify the proposcd crushing and cracking plasticity models, the experiments have load capacities govc11.nc.d either by compressive crushing of'concrete or by yi~lding of' reinforcing steel.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.31 no.5
• /
• pp.1031-1043
• /
• 2021

Recently, the importance of information security has become greater. For this reason, the number of departments related to information security at universities and professional educational institutions is increasing. It is analyzed that the curriculum of such educational institutions has a lot to do with the job competency mentioned in NCS in relation to major subjects. This has the advantage of providing a standardized level of education for each job competency. However from the perspective of students there may be limitations on duplicate curriculum quality of education according to the level of teachers and timely acquisition of necessary elemental skills. In this paper we analyze the curriculum of universities and professional educational institutions and propose a cell-based three-dimensional education model to address the limitations of students. We verified based on the college curriculum that the proposed model can effectively improve its limitations.