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Absorption Characteristics of Puffed Red Ginseng Lateral Root, Red Ginseng Main Root and White Ginseng Lateral Root Powder (팽화 홍미삼, 홍삼절편 및 백미삼 분말의 흡습특성)

  • Kim, Sang-Tae;Youn, Kwang-Sup;Kwon, Joong-Ho;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.30-36
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    • 2008
  • In this study, raw ginseng produced by different method; was puffed and the absorption characteristics of the puffed ginseng powders were investigated Raw ginseng preparations, including white ginseng lateral root (WGL), red ginseng lateral root (RGL) and red ginseng main root (RGM) with 15 % moisture were puffed at a pressure of $7kg_f/cm^2$. The equilibrium moisture contents of puffed powders were affected by temperature and water activity. The monolayer moisture content determined by 1he BET equation was 0.034-0.045g $H_2O/g$ solid. The $R^2$ parameter of 1he BET equation was higher than that of the GAB equation. The absorption enthalpies, calculated using various water activities, showed a decreasing trend with increasing water activity. Amongst models applied for predicting equilibrium moisture content, 1he Kuhn model was 1he best fit for puffed ginseng powders, giving 1he lowest prediction deviation of 2.83-8.65% The prediction model equation for water activity included 1he variable of time, water activity (RH/l00) and temperature, whereas an equation featuring the parameters of time and water activity was the best model equation identified.

In vitro studies of anti-inflammatory and anticancer activities of organic solvent extracts from cultured marine microalgae

  • Samarakoon, Kalpa W.;Ko, Ju-Young;Shah, Md. Mahfuzur Rahman;Lee, Ji-Hyeok;Kang, Min-Cheol;Kwon, O-Nam;Lee, Joon-Baek;Jeon, You-Jin
    • ALGAE
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    • v.28 no.1
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    • pp.111-119
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    • 2013
  • Marine microalgae are a promising source of organisms that can be cultured and targeted to isolate the broad spectrum of functional metabolites. In this study, two species of cyanobacteria, Chlorella ovalis Butcher and Nannchloropsis oculata Droop, one species of bacillariophyta, Phaeoductylum tricornutum Bohlin, and one species of Dinophyceae, Amphidinium carterae (Hulburt) were cultured and biomasses used to evaluate the proximate comical compositions. Among the determined proximate chemical compositions of the cultured marine microalgae, the highest content of crude proteins and lipids were exhibited in P. tricornutum and A. carterae, respectively. Solvent-solvent partition chromatography was subjected to fractionate each of the cultured species and separated n-hexane, chloroform, ethyl acetate, and aqueous fractions. Nitric oxide production inhibitory level (%) and cytotoxicity effect on lipo-polysaccharide-induced RAW 264.7 macrophages were performed to determine the anti-inflammatory activity. N. oculata hexane and chloroform fractions showed significantly the strongest anti-inflammatory activity at $6.25{\mu}g\;mL^{-1}$ concentration. The cancer cell growth inhibition (%) was determined on three different cell lines including HL-60 (a human promyelocytic leukemia cell line), A549 (a human lung carcinoma cell line), and B16F10 (a mouse melanoma cell line), respectively. Among the extracts, C. ovalis ethyl acetate and A. carterae chloroform fractions suppressed the growth of HL-60 cells significantly at 25 and $50{\mu}g\;mL^{-1}$ concentrations. Thus, the cultured marine microalgae solvent extracts may have potentiality to isolate pharmacologically active metabolites further using advance chromatographic steps. Hence, the cultured marine microalgae can be described as a good candidate for the future therapeutic uses.

Assessment of Soil and Water Quality in some Catchments of Different Agricultural Practices in Nakdong River Basin (낙동강 유역 농업지대에서 영농형태별 토양과 수질 평가)

  • Kim, Min-Kyeong;Seo, Myung-Chul;Lee, Nam-Jong;Chung, Jong-Bae;Kim, Bok-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.1
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    • pp.16-23
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    • 2003
  • Soil and water quality was monitored in the Nakdong River basin to assess the impact of different agricultural practices. From five catchments, soil samples were collected at three times during 1996 to 1998, and water samples were collected at twelve times on July during 1995 to 1999. The major agricultural practices were paddy and upland farming in three areas surveyed: Youngju, Goryung, and Milyang. Apple orchards were located along in the Imgo-Cheon catchment. Intensive vegetable farming in plastic fIlm house was practiced in the Habin-Cheon catchment. Total N contents, 0.04-0.32%, of paddy soils were low in comparison with those of upland, orchard, and plastic film house soils. Available phosphate($P_2O_5$) contents, $2-421mg\;kg^{-1}$, in plastic film house soils were higher than those in paddy soils. In plastic film house and upland soils, CEC of soils were high. The N concentrations in most of the streams were higher than $1.0mg^{-1}$, the standard of agricultural irrigation water. The P concentrations were above $1.0mg^{-1}$, the standard of agricultural irrigation water and were higher than the minimum level for eutrophication, $0.01-0.05mg\;L^{-1}$ in most of the streams. In conclusion, nutrients by agricultural activity could affect water quality of streams near the agricultural fields. Good water quality in streams can be maintained by proper management of agricultural fields and by decreasing application amount of fertilizers in agricultural fields.

Kinetic Analysis of Cathepsin B Inhibitor Using a Spectrophotometric Assay (분광광도법에 의한 Cathepsin B 저해물질의 효소동력학적 저해특성 조사)

  • 한길환;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.90-95
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    • 2001
  • Kinetic Analysis of Cathepsin B Inhibitor Using a Spectrophotometric Assay. Han, Kil-Hwan and SangDal Kim*. Department of Applied MicrobioJ0f5Yt Yeungnam UniversitYt Kyongsan 77 2-749, Korea - The KHS 10, C4Hl10~6 formula produced from Streptomyces luteogriseus KT-] 0 effectively inhibited a lysosomal cysteine proteinase, cathepsin B. It inhibited the enzyme activity of cathepsin B competitively when the N a-CBZ-Llysine p-nitrophenyl ester HC] (CLN) was used as a substrate. The inhibition const:mt (Ki) of KHS 1 0 for cathepsin B detennined by spectrophotometeric assay was 430 nM. The effective inhibition of cathepsin B was observed at $25^{\circ}C$ :md pH 6.0. The cathepsin B inhibitor, KHSlO needed a preincubation of cathepsin B with the inhibitor for over 5 min. The KHS 10 preserved over 80% inhibition activity even after heat-treatment at $100^{\circ}C$ for ] hr.

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A study on the railroad library under the Japanese rule (철도도서관에 관한 고찰 -특히 일제하의 도서관봉사활동을 중심으로-)

  • 김포옥
    • Journal of Korean Library and Information Science Society
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    • v.9
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    • pp.63-83
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    • 1982
  • Among many open libraries under the rule of the Japanese imperialism, only the railroad library as the character of special library under the control of the Japanese Government General is remarkable for having opened its facilities to outsiders as well as the interior users. This study tries a) To look into what were the colonial background, aim and motive of the establishment of the railroad library under the Japanese rule, b) To analyze its activities and the change of its organization, c) And to find out its vestiges an the effects which it has on our country's library circles. The following are the result: A. The railroad library under the rule of the Japanese imperialism functioned as a public library by the outside book's circulation rather than the interior using. B. The outsiders were qualified to use it only when they got the joint guarantee for the city resident, which means that the o n.0, pportunity of the circulation was not free for the Koreans rather than the Japanese. C. From the aspect of collections, technical books such as natural science, and reference ones tended to be collected, and their whole composition was focused on the materials for the Japan-Korea identification scheme and development of the national spirit as the Japanese empire's subjects. D. In the respect of its organization, all the clerical employees, as well as the chief librarian were composed of the Japanese. Although the Koreans were in principle prohibited from working there, a few of them were employed as guard or servant as the China-Japan War was close to the end. E. The area of the outside book's, circulation service was expanded throughout the whole region along the railroad connecting Pusan with Manchuria. Such a service activity which contained several meanings, was tried by means of culture's books, circuit's books, train's books, hotel's books, mind's development books and reader's reading books etc. F. Such matters as the limitation on book circulating and the quantity of circulation book, the paid circulation and the partial restriction on free circulation mean the wartime system under the rule of the Japanese imperialism and also a n.0, ppeared as a factor of hampering the Koreans from getting accustomed to using library, which has affected them until now. G. The noticeable thing in the service activity is shown in attempting the diversified and positive way of service like free circulation in order to solidify the attitude of the Koreans toward war by organizing mind's development books as well as culture's books with the China-Japan War. H. On the other hand, it can not be passed over that the diversified service activity of the railroad library played a role of bridge in turning of today's library circles of ours to the modernization.

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Mineral Metabolism in Singleton and Twin-pregnant Dairy Goats

  • Harter, C.J.;Castagnino, D.S.;Rivera, A.R.;Lima, L.D.;Silva, H.G.O.;Mendonca, A.N.;Bonfim, G.F.;Liesegang, A.;St-Pierre, N.;Teixeira, I.A.M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.1
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    • pp.37-49
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    • 2015
  • During pregnancy, the maternal body undergoes significant physiological changes. The present study assessed the changes on calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na) and potassium (K) metabolism in singleton and twin-pregnant dairy goats. The 42 goats used ($49.5kg{\pm}7.6$ body weight [BW]) were assigned at random to treatments that were factorially arranged to account for 2 breeds (Oberhasli and Saanen), 2 pregnancy types (singleton and twin) and 3 gestation periods (80, 110, and 140 days). Digestibility trials were performed at 80, 110, and 140 days of gestation. Mineral retention during pregnancy was determined in the maternal body, femur, uterus, mammary gland, fetus and fetal fluid. Blood samples were taken during pregnancy before and after a meal, and Ca, P, Mg, Na, K ions and alkaline phosphatase activity determined in serum. Bone mineral density was determined in the right femur. Statistical analyses were performed using the SAS MIXED procedure. Dry matter intake decreased linearly up to 140 days of gestation. Maternal BW gain, and Ca, P, and Mg retention (g/kg) decreased linearly with the advance of gestation days. Macromineral retention in maternal body (g/kg) was greater in Oberhasli than Saanen goats, and their fetuses had higher Ca, P, and Mg deposition (mg/g). Mineral retention (mg/g) increased in fetuses according to pregnancy development, with no differences between singleton and twin pregnancy. In the mammary gland, the retention of all minerals (g) increased with the days of pregnancy. In conclusion, related to Ca, P, and Mg metabolism can be divided into two stages. Up to 80 days of gestation, was characterized by the preparation of the maternal body reserves for future mineral demands. From 80 days of gestation onward, was characterized by the transfer of maternal body reserves for fetal development and colostrum production. Na and K supply was provided by adjustments in endogenous excretion and an increase in intestinal absorption. Finally, mineral metabolism was specific to each genotype and, except for Na, was not affected by the number of fetuses.

Si 함유 다이아몬드상 카본 필름의 환경 변화에 따른 마찰거동 연구

  • 박세준;이광렬;공호성;양승호
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.126-126
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    • 2000
  • 다이아몬드상 카본(DLC) 필름은 경도가 높고, 마찰계수가 낮다는 장점을 가지고 있기 때문에 내마모성 코팅이나 윤활성코팅에 응용을 위한 연구가 활발히 진행중이다. 하지만 마찰계수가 주변환경에 매우 큰 영향을 받는다는 단점이 있다. 이러한 단점은 DLC필름의 응용에 대한 저해 요인이 되며, 이 점을 보완하기 위해서 DLC 필름에 Si을 첨가하는 연구들이 진행되고 있다. 본 실험에서는 r.f-PACVD 법을 이용하여 Si이 첨가된 DLC 필름의 주위 환경 변화에 따른 마찰특성의 변화를 연구하였다. 사용한 반응 가스는 벤젠(C6H)과 희석된 Silane(SiH4 : H2 = 10 : 90)이며, 희석된 Silane과 벤젠의 첨가비율을 조절하여 필름내 Si의 함량을 조절하였고, 증착시 바이아스의 전압은 -400V로 하였다. 마찰테스트는 Ball-on-Disk type의 조건에서 대기, 건조공기, 진공의 세가지 분위기에서 마찰테스트를 실행하였다. 실험결과 마찰계수는 건조공기, 대기, 진공의 순으로 증가하였고, 필름내에 포함되어 있는 Si의 양이 증가할수록 마찰계수는 낮고 안정한 값을 나타내었다. Tribochemiacal 분석과, ball과 track의 전자현미경 사진 분석 결과, 진공에 비해서 건조공기와 대기중에서 마찰계수가 낮은 것은 DLC 필름내에 마모 track 중심부에 Si-C-O 계의 화합물이 형성되어, 이 화합물이 마찰계면에 존재하여 마찰계수를 낮추었음을 확인하였다. 그리고 대기중에서 실험한 경우, 습기의 존재로 인해 마모입자가 볼의 표면에서 엉김으로써 건조공기의 상태에서 보다 높은 마찰저항을 갖게 됨으로 인하여 마찰계수가 높아짐을 알 수 있었다.a)는 as-deposit 상태이며, 그림 1(b)는 45$0^{\circ}C$, 60min 열처리한 plan-view TEM 사진이다.dical의 영향을 조사하였으며 oxygen radical의 rf power에 따른 변화는 OES(Optical emission spectroscopy)를 사용하였다. 너무 적은 oxygen ion beam flux나 oxygen radical은 film의 전도도 및 투과도를 저하시켰고 반면 너무 과도한 flux의 증가 시는 전도도는 감소하였고 투과도는 증가하는 경향을 보였다. 기판에 도달하는 oxygen ion flux는 faraday cup을 이용하여 측정하였으며 증착된 ITO film은 XPS, UV-spectrometer, 4-point probe를 이용하여 분석하였다. 때문으로 생각되어진다. 또한, 성장 온도가 낮아짐에 따라 AlGaN의 성장을 저해하기 때문으로 판단된다. 성장 온도 변화에 따라 성장된 V의 구조적 특성 및 표면 거칠기 변화를 관찰하여 AlGaN의 성장 거동을 논의하겠다.034, 0.005 정도로 다시 감소하였다. 박막의 유전율은 약 35 정도의 값을 나타내었으며 X-선 회절 data로부터 분석한 박막의 변형은 증온도에 따라 7.2%에서 0.04%로 감소하였고 이 이경향은 유전손실은 감소경향과 일치하였다.는 현저하게 향상되었다. 그 원인은 SB power의 인가에 의해 활성화된 precursor 분자들이 큰 에너지를 가지고 기판에 유입되어 치밀한 박막이 형성되었기 때문으로 사료된다.을수 있었다.보았다.다.다양한 기능을 가진 신소재 제조에 있다. 또한 경제적인 측면에서도 고부가 가치의 제품

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The variational characteristics of Water Quality and Chlorophyll a Concentration in Kogum-sudo, Southern Part of Korean Peninsula (거금수도 양식어장의 해양환경 특성 2. 수질과 엽록소 양의 변동특성)

  • 윤양호;박종식;고남표
    • Journal of Aquaculture
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    • v.13 no.1
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    • pp.87-99
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    • 2000
  • Field survey on the variational characteristics of water quality and chlorophyll a concentraion were carried out at the 25 stations for four seasons in Kogum-sudo(Straits) southern coast of Korean Peninsula from Feburuary to October in 1993. We made an analysis on biological factor as chlorophyll a concentraion as well as physicio-chemical factors such as water temperature salinity sigma-t transparency dissolved oxygen(DO) chemical oxygen demand(COD) nutrients (ammonia, nitrite, nitrate, phosphate, and silicate) N/P ratio and suspended solid(SS). The waters in the Kogum-sudo were not stratified due to the tidal mixing. And the high productivity in photic layer were supported by high nutrients concentration from bottom waters. The high concen-trations of suspended solid in straits had a bad influence upon marine biology of nature and cultivations. In Kogum-sudo had a sufficient nutrients for primary productivity during a year. Especially phosphate and inorganic nitrogen were high the other side silicate was very low. The source of nutrients supply depend on rather mineralization of organic matters and input of seawater from outside than input of freshwaters from lands. Phytoplankton biomass as measured by chlorphyll a concentratiion was very high all the year round and it was controlled by the combination o f several environmental factors especially of phosphate in summer and dissolved nitrogen in other seasons.

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Thermal Insulation and Flame Retardant Properties of Cement Based Super Light-weight Inorganic Thermal Insulation using 100㎛ Grade Glass Bubble (100㎛급 글라스 버블 혼입 시멘트계 초경량 무기 단열재의 단열 및 난연특성)

  • Son, Bae-Geun;Song, Hun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.9 no.4
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    • pp.642-649
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    • 2021
  • Energy saving standard for buildings are strengthened, the application of exterior insulation finishing system and thickness of insulation materials are increasing. Most buildings with exterior insulation finishing system is applied organic insulating material. Organic insulating material have workability, economic feasibility, reduction in construction cost, and excellent thermal insulation performance. However, Organic insulating material is very vulnerable to heat, so when a fire occurs, rapid fire spread and toxic gas are generated, causing many casualties. Inorganic insulating material can be non-combustible performance, but it is heavy and has low thermal insulation performance. Mineral wool has higher thermal insulation performance than other types of inorganic insulating material, but mineral wool is disadvantageous to workability and vulnerable to moisture. Glass bubble are highly resistant to water and chemically stable substances. In addition, the density of the glass bubble is very low and the particles are spherical, fluidity is improved by the ball bearing effect. Glass bubbles can be used with cement-based ino rganic insulating material to impro ve the weight and thermal insulatio n perfo rmance o f cement-based inorganic insulation. This study produced a inorganic insulating materials were manufactured using cement-based materials and glass bubble. In order to evaluate the insulation performance and flame retardant performance of cement-based super light-weight inorganic insulating materials using with glass bubble, insulation performance or flame retardant and non-combustible performance were evaluated after manufacturing insulating materials using micro cement and two types of glass bubbles. From the test result, Increasing the mixing ratio of glass bubbles improved the insulation performance of cement-based super light-weight inorganic insulating materials, and when the mixing ratio of glass bubbles was 10%, it sho wed sufficient flame retardant and no n-co mbustible perfo rmance.

Isolation of 20(S)-Ginsenoside Rg3 and Rg5 from the Puffed Red Ginseng (팽화 홍삼으로부터 20(S)-Ginsenoside Rg3와 Rg5의 분리 및 구조동정)

  • An, Young-Eun;Cho, Jin-Gyeong;Baik, Nam-In;Choi, Sung-Won;Hur, Nam-Yoon;Park, Seok-Jun;Kim, Byung-Yong;Baik, Moo-Yeol
    • Food Engineering Progress
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    • v.14 no.2
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    • pp.159-165
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    • 2010
  • Red ginseng tail roots (9.8 g water/100 g sample) were puffed at 7, 8, 9, and 10 $kg_{f}/cm^{2}$ using a rotational puffing gun. Puffed red ginseng was extracted with 70% ethanol, and the concentrated extract was successively partitioned with diethyl ether, n-butanol and $H_{2}O$. Two unknown ginsenosides from puffed red ginseng were found at 63 and 65 min of retention time in HPLC chromatogram suggesting that chemical structure of some ginsenosides might be altered during the puffing process. Identification of two unknown compounds was carried out using TLC, HPLC and NMR. Two major compounds were isolated from TLC. According to TLC result, compound I was expected to be the mixture of ginsenosides Rk1 and Rg5, and compound II was expected to be a 20(S)-ginsenoside $Rg_{3}$. Three compounds were isolated from n-butanol fraction through repeated silica gel and octadecyl silica gel column chromatographies. From the result of $^{1}H$- and $^{13}C$-NMR data, the chemical structures of unknown compounds were determined as ginsenoside $Rg_{5}$ and 20(S)-ginsenoside $Rg_{3}$. Unfortunately, ginsenoside $Rk_{1}$ could not be separated from ginsenoside-$Rg_{5}$ in the compound I. It was carefully reexamined using HPLC and confirmed that the last unknown compound was ginsenoside-$Rk_{1}$.