• 제목/요약/키워드: N. orientalis

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백자인(Thuja orientallis L.)으로부터 tyrosinase 저해제의 분리 및 정제 (Isolation and Purification of Tyrosinase Inhibitors from the Seeds of Thuja orientalis L.)

  • Lee, Jun-Young;Park, Sang-Won
    • 한국식품저장유통학회지
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    • 제7권4호
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    • pp.409-413
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    • 2000
  • 식물추출물로부터 보다 안전하고 효과있는 새로운 tyrosinase 저해제를 개발하기 위한 연구의 일환으로 먼저 기존의 spectrophotometric assay를 개선한 새로운 TLC 검색방법을 사용하여 여러 식물추출물을 검색한 결과 백자인의 메탄올추출물이 강한 tyrosinase 저해활성을 갖고 있음을 알 수 있었다. 따라서 백자인의 메탄올추출물로부터 tyrosinase 저해제를 분리하기 위하여 먼저 탈지 메탄올추출물을 에테르, 에틸아세테이트 및 부탄올로 순차적으로 용매분획한 후 이 중 활성이 강한 에테르 추출물을 얻었다, 다음 에테르 추출물을 5% NaHCO$_3$로 씻은 후 산성화하여 얻은 강산성에테르추출물을 silica gel, Sephadex LH-20 및 분취 TLC를 각각 이용하여 4가지의 tyrosinase 저해물질을 분리하였으며, 이 중 화합물 2 ($IC_{50}$/=26 $\mu\textrm{g}$/ml)와 3 ($IC_{50}$/=30 $\mu\textrm{g}$/ml)은 tyrosinase 저해제로 잘 알려진 ascorbic acid ($IC_{50}$/=28 $\mu\textrm{g}$/ml)와 유사하게 강한 tyrosinase 저해활성을 나타내었으며, 현재 그들의 화학구조를 동정하고 있다. 이러한 결과를 미루어볼 때 백자인으로부터 분리된 tyrosinase 저해제는 식품의 효소적갈변저해제로써 뿐만 아니라 기미, 주근깨 및 검반점을 예방할 수 있는 미백화장품 및 의약품의 신소재로써 활용될 수 있을 것으로 기대된다.

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반폐쇄적 여자만 동물플랑크톤 군집의 계절변화에 따른 환경요인의 영향 (Effects of Environmental Factors on the Seasonal Variations of Zooplankton Communities in the Semi-enclosed Yeoja Bay, Korea)

  • 문성용;김희용;이미희;정진호;유세라
    • 한국수산과학회지
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    • 제56권1호
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    • pp.54-65
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    • 2023
  • Effect of environmental factors on the seasonal variations of zooplankton communities was investigated in the semi-closed Yeoja Bay, Korea from February, April to August, and November 2021. Out of a total 49 species of zooplankton were collected with a predominant of neritic copepods (mainly Paracalanus orientalis, Acartia omorii, Acartia ohtsukai, Centropages abdominalis, Ditrichocorycaeus affinis, and Oithona sp.), accounting for 58.9% of the total abundance of zooplankton. The diversity indices indicated a relatively highest in July, suggesting that diversity is influenced by seasonal temperature, N. scintillans, and neritic copepods species. A cluster analysis with non-metric multidimensional scaling revealed four groups of zooplankton communities. The February sample clustered into Group A, having the lowest mean total abundance and species diversity of zooplankton, consisting mainly of N. scintillans located the whole region. Cluster Group B from the spring season (April to May) and contained the species diversity with some neritic copepods. Cluster Group C from the summer season (June to August) mainly comprised P. orientalis, A. ohtsukai, Oithona sp., and hydromedusae. Cluster Group D from the autumn season (November) mainly comprised P. orientalis, Temora discaudata. Redundancy analysis indicated that abundance is positively correlated with temperature, salinity, and pico chlorophyll-a concentrations. This study showed that planktonic larvae (such as branchyura larvae) and some copepods (including A. omorii, A. ohtsukai, C. sinicus, and C. abdominalis) were significantly vulnerable to zooplankton community of temperature, salinity, and pico chlorophyll-a concentrations.

EVALUATIN OF NALITA WOOD(Trema orientalis) AS SOURCE OF FIBER FOR PARERMAKING

  • ;문성필
    • 한국펄프종이공학회:학술대회논문집
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    • 한국펄프종이공학회 2003년도 추계학술발표논문집
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    • pp.17-26
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    • 2003
  • Nalita wood(Trema orientalis) is one of the fastest growing woods in the world. It may be a viable species for pulpwood. The physical, chemical and morphological properties of Nalita were studied. The total lignin, pentosan and holocellulose content in Nalita wood were 24.7%, 22% and 81.2%, respectively. Its fiber length was about 0.92 mm, which are comparable to Acacia mangium. Nitrobenzene oxidation of Nalita wood meal indicated that the guaciayl and syringyl unit were the major constituent of Nalita lignin. Nalita produced 50% pulp yield at Kappa number 21 in soda-anthraquinone process. The strength properties of Nalita pulp were comparable to other tropical hardwood pulp. At $40_{\circ}$ SR, the breaking length, burst index, tear index and total energy absorption were 6000 m, $3.5kPa{\cdot}m^2/g\;and\;7.0mN{\cdot}m^2/g\;and\;75J/m^2$, respectively.

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Inhibitory Effects of the Essential Oils on Acetaminophen-Induced Lipid Peroxidation in the Rat

  • Choi, Jong-Won;Lee, Kyung-Tae;Jung, Won-Tae;Jung, Hyun-Ju;Lee, Seung-Hyung;Park, Hee-Juhn
    • Natural Product Sciences
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    • 제8권1호
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    • pp.18-22
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    • 2002
  • Inhibitory effects of the essential oils obtained from ten herbs were tested on acetaminophen-induced lipid peroxidation in the rat. The oil of Artemisia princeps var. orientalis buds (AP-oil) showed the most significant hepatic malondialdehyde value which was comparable to those of ascorbic acid and methionine. This was warranted by the protective effect on hepatic glutathione depletion. Overview of the data on the activities of hepatic microsomal enzymes, aminopyrine N-demethylase and aniline hydroxylase led to the notice that the suppressed activities of those enzymes are mainly responsible for the anti-lipid peroxidation. The interpretation of GC-MS data on the AP-oil revealed the ingredient of cineol, thujone, carvone, borneol, camphor and terpineol.

한국산 우묵날도래 과와 가시날도래 과의 분류학적 검토 (Taxonomic Notes on Limnephilidae and Goeridae (Trichoptera: Limnephiloidea) of Korea)

  • 최현정;;우건석
    • Animal Systematics, Evolution and Diversity
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    • 제15권1호
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    • pp.27-49
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    • 1999
  • 남한에 분포하는 우묵날도래 과의 기시날도래 과에 속하는 15종을 보고하며, 성충 동정에 필요한 기재문과 외부생식기의 도해, 건조표본의 사진 그리고, 지리적 분포에 대한 정보를 제공한다. 이 중, 슈미드우묵날도래 Brachpsyche schmidi sp. n.는 신종이며, 동야모시우묵날도래(신칭) Limnephilus orientalis Martynov와 방동가시날도래(신칭) Goera curvispina Martynov등 2종은 한국에서 처음으로 보고된다. 그리고, 어리우묵날도래(신칭) Nemotalius mutatus MacLachlan, 아무르검은날개우묵날도래(신칭) Asynarchus amurensis Ulmer, 우리큰우묵날도래(신칭) Hydatophylax formosus Schmid등 3종은 남한미기록종이다.

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장봉도 갯벌의 쥐노래미(Hexagrammos otakii)와 조피볼락(Sebastes schiegeli)의 섭식생태 (Comparative Feeding Ecology of Sympatric Greenling Hexagrammos otakii and Schlegel's Black Rockfish Sebastes schlegeli in the Jnngbong Tidal Flat, Incheon, Korea)

  • 서인수;홍재상
    • 한국수산과학회지
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    • 제40권2호
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    • pp.84-94
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    • 2007
  • The comparative feeding ecology of greenling Hexagrammos otakii and Schlegel's black rockfish Sebastes schlereli populations was investigated in the Jangbong tidal flat near Incheon, Korea. Monthly samples were taken using a modified otter trawl from November 1999 to January 2001. The stomach contents of 124 greenling and 115 Schlegel's black rockfish were analyzed. The diet of H. otakii was dominated by the amphipods Gammaropsis iaponicus, Caprella scaura, Isaeopsis sp. and Monocorophium acherusicum, the shrimps Latreutes mucronatus and Alpheus japonicus, the mysid Neomysis orientalis and the bivalve Mytilus edulis. In contrast, S. schlegeli mainly fed on the mysids N. orientalis, N japonica, and N. awatschensis, the shrimps Exopalaemon carinicauda, A. japonicus and L. mucronatus, and the fish Pholis fangi and unidentified gobiids. A comparison of the frequency and abundance of food items showed that crustaceans (e.g. amphipods, mysids and shrimps) were important foods for both species. Their main dietary components, however differed which is probably due to differences in the spatial distribution of the two species and their prey items. In conclusion, greenling and Schlegel's black rockfish, despite being sympatric on a tidal flat have distinct food habits and low dietary overlap (Schooner's index: 0.1). These results indicate, to some extent, resource partitioning for the maximum utilization of available foods in the tidal flat.

무미양서류의 도약속도와 다리근육의 동력 및 수축속도와의 관계 (Relationship of Maximal Take-off Speed to Power and Shortening llelocitv of Hindlimb Muscle in Anuran Amphibians)

  • 최인호
    • 한국동물학회지
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    • 제39권2호
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    • pp.132-138
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    • 1996
  • To learn how maximal locomotory speed of animals is defined in terms of hindlimb structure and muscle contractile function, take-off speed, hindlimb length, thigh muscle mass, shortening velocity and power of the sastrocnemius muscle were measured with one fast species, Rono nigromaculota and one relatively slowresponding species, Bombina orientalis. Take-off speed (m.sec-1) was greater in R. nigromoculata $(2.4\pm0.2SD, $ n: 14) than in the Bombino $(1.6\pm0.1SD, $ n=8). Stvle of the take-off response was a long-iump type in the Rano and a short-ranged hopping in the Bombing. Faster take-off capacity of the ranid frogs was supported by the longer hindlimb length (relative to body length) and the more massive thigh muscles (relative to body mass), compared to the Bombina. Further, the ranids exhibited faster maximal shortening velocity and Breater maximal power generateion than the Bombina [Vmax $(ML.sec-1)=11.79\pm1.69SD$ for the Runa and $9.74\pm1.27SD$ for the Bombina; Pmax $nW.kg-1)=222.42\pm42.42SD$ for the Rono and $169.03\pm34.52SD$ for the Bombinal. With more massive thigh muscles and greater mechanical power, the ranids would generate greater total power and thus higher energy release per unit time to muscle tissues for the burst take-off. As a consequence, biomechanical properties seen in the ranids seem to be more effective for frost take-off than in the Bombina.

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한국에 있어서 벼기생선충에 관한 연구 III. 벼기생선충 종류 및 분포조사 (Nematodes Associated with Rice in Korea III. Survey on Nematode Species and Distribution Associated with Rice)

  • 최영연;박상봉;송철;최영식;박효숙;정희창
    • 한국응용곤충학회지
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    • 제28권3호
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    • pp.120-145
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    • 1989
  • 1985년부터 3개년에 걸쳐서 전국 387개 시.읍.면의 1, 160개 논에서 벼기생선충을 조사한 결과 우리나라 논에서 벼와 관련된 선충은 35종이 밝혀졌으며 본 조사로 26종이 새로이 추가되었고 세계적으로는 Criconemoides informis, Criconemella paragoodeyi, Criconema (Criconema) jaejuense, Ditylenchus longicauda, Eutylenchus africanus, Merlinius n. sp., Meiodorus n. sp., Ogma (Homogma) serrata, Ogma (Homogma) insulicum, Orientylus orientalis, Paratylenehus lepidus, Pararotylenchus Pini, Triνersus n. sp., Tylenchorhynchus claνicaudatus, Tylench-orhynchus nudus등 15종이 벼기생선충으로 새로이 기록되었다. 우리 나라에서 처음으로 발견된 Criconemella paragoodeyi, Ditylenchus longicauda 및 Merlinius n. sp. 등은 전국적으로 분포 하고 있을 뿐 아니라 밀도 또한 높으므로 벼의 중요한 기생선충으로 사료된다. 벼뿌리선충은 전국적으로 분포하며 밀도 또한 높았고, 애벼뿌리선충은 남부지방에서는 벼뿌리선충과 같이 분포되고 있으나 북부지방으로 갈수록 점차 밀도가 낮아지는 경향을 나타냈다

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DNA 추출없이 전혈을 이용한 PCR-전기영동법에 의한소의 타일레리아병 진단 (Diagnosis of Bovine Theileriosis by Direct PCR and Electrophoresis from Whole Blood Without DNA Extraction)

  • 강성호;장상민;채준석;김용성
    • 대한화학회지
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    • 제47권2호
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    • pp.127-132
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    • 2003
  • 소의 타일레리아병 진단을 위해 DNA 추출과정 없이 전혈에서 바로 중합효소연쇄반응(PCR)을 통하여 T. buffeli(buffeli/orientalis/sergenti)의 16S rRNA 유전자 단편을 증폭시킨 뒤, 증폭된 DNA를 전기영동법으로 분석하는 방법을 개발하였다. 특이유전자 단편의 증폭을 위해 formamide를 사용하여 혈액세포를 용해시켰으며, 단백질의 응고를 줄이기 위해 낮은 반응온도를 사용하는 FoLT(Formamide Low Temp.) PCR법을 이용하였다. 전혈 100-200 nL를 바로 PCR 증폭에 사용하였으며, PCR 산물(816-bp DNA)은 전기영동법으로 분석하였다. 본 결과는 T. buffeli에 감염된 소의 혈액으로부터 정제된 DNA를 사용하여 얻은 실험결과와 잘 일치하였다.