• Animal Systematics, Evolution and Diversity
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• v.13 no.2
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• pp.93-102
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• 1997

Two new marine tardigrade species, Batillipes longispinosus n. sp. and B. orientalis n. sp. are described on the basis of the specimens collected from the intertidal or shallow sublittoral sands of South Korea. Both the present species are characteristic in the absence of any caudal appendage throughout their life span, and bearing the long clava or cephalic appendages with the long spinous process on the femur of legs IV. B. longispinosus n. sp. most resembles B. tuvernatis Pollock, but is easily distinguished from it by the flattened caudal region and prominently developed spine of legs IV. B. orientalis n. sp. is much related to B. roscoffensis Kristensen, in having the wing-formed lateral body prohection between legs III and legs IV, the papillae on the head region, and the smooth caudal region, but discernible from it in the general body shape and the relatively longer spine of legs IV.

• Animal Systematics, Evolution and Diversity
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• v.26 no.1
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• pp.49-54
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• 2010

Paramolgus orientalis n. sp. is described as an associate of the antipatharian coral Antipathes japonicus from Jeju Island, Korea. The new species is distinguished from its three relatives P. constrictus, P. delicatulus, and P. insectus, all known from antipatharians in tropical waters, by its long caudal ramus which is more than four times as long as wide, by the possession of 4 setae on maxillule, and by other morphological differences in the antenna and maxilla.

• Korean Journal of Plant Taxonomy
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• v.39 no.2
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• pp.107-113
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• 2009

We report Najas orientalis Triest & P. Uotila and N. oguraensis Miki as unrecorded species from the Korean flora. N. orientalis is distinct from other Najas species in having rounded leaf sheaths and raised testa in the cell walls, and in that this species occurs in the southern region of Korea (Gimhae, Gangjin). N. oguraensis is close to N. minor, but is distinct from this species by having larger male flowers and 4-celled anthers. This species is distributed in the southern region of Korea (Changwon, Jangheung). We also give a new Korean common name for N. gracillima (A. Braun ex Engelm.) Magnus, which is widely distributed in South Korea. A taxonomic key to the six species of Najas is given.

• Korean Journal of Environmental Biology
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• v.22 no.2
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• pp.300-307
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• 2004

The chloroacetanilide herbicide alachlor (2-chloro-2', 6'-diethyl-N-(methoxymethyl)-acetanilide) and organochlorine insecticide endosulfan (6, 7, 8, 9, 10, 10-hexachloro-l, 5, 5a, 6, 9, 9a-hexahydro-6, 9-methano-2, 3, 4-benzodioxathiepin-3-oxide)are the highly toxic agricultural chemicals. Bombina orientalis is one of the most common amphibians in the world and comprises a large proportion of their total number. B. orientalis spawns in the farming regions at Spring when the massive application of agricultural chemicals occurs. These chemicals in farmland may threaten the reproduction of this frog. Therefore, we examined the embryotoxic and survival effects of alachlor and endosulfan at various concentrations in B. orientalis embryos. The survival rates of embryos at 312h post fertilization treated with alachlor and endosulfan were decreased with concentration dependent manner. Also, developmental malformations appeared by alachlor and endosulfan in B. orientalis embryos. The malformations showed in order of frequency with bent trunk, tail dysplasia, bent tail, thick-set body and ventral blister in alachlor treated embryos. The exposure of endosulfan produced 7 types of severe external malformations with tail dysplasia, pectoral blister, bent trunk, bent tail, cephalic dysplasia, ventral blister, and thick-set body. Following exposure to alachlor and endosulfan the types of malformations were diverse, suggesting these chemicals target multiple events in embryonic and larval development in this species. These results suggested that alachlor and endosulfan were detrimental for survival and development of B. orientalis embryos.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.136-144
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• 2007

Biotae Orientalis has been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone and MeOH extracts of dried or fresh Biotae Orientalis, on hair growing activity of the C57BL/6N mice after topical application to skin. First, We examined on hair growth activity of extracts of Biotae Orientalis compare to control and 1% minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Biotae Orientalis to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. I fourth investigated changes of subpopulation of splenocytes and thymocytes in C57BL/6N mice for 16day using laser flow cytometry. The results were as follows : Hair growing effect of acetone and MeOH extracts of dried and fresh Biotae Orientalis was observed in 70%, 90% and 60% in hair removed skin area in 16 day respectively. Immunoreactive density of VEGF and PKC-${\alpha}$ in skin of experimental groups was weakly stained compare to control group in 10 day. Immunoreactive density of stem cell factor in skin of experimental group was heavily stained compare to control group in 10 day. Splenic TH/TC Iymphocytes of lived MeOH extracts group significantly increased compare to control group. TH cells in thymic T lymphocytes were increased compare to control group. These experiment suggest that acetone and MeOH extracts of Biotae Orientalis may be used for topical treatment of alopecia areata.

• Korean Journal of Medicinal Crop Science
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• v.18 no.5
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• pp.315-322
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• 2010

This study was carried out to investigate the antimicrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The native fruits in Korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, and butanol. The fraction yields of n-hexane, chloroform, ethylacetate, butanol and water of ethanol extract were 10.15%, 10.05%, 1.45%, 45.35% and 27.55%, respectively. n-Hexane-soluble fraction showed the highest antibacterial activity against gram positive bacteria, while the chloroform, ethylacetate, butanol and aqueous fractions did not show any antibacterial activity. Minimum inhibitory concentration (MICs) on Staphyloycoccus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction were $100\;{\mu}g$, $500\;{\mu}g$ and $50\;{\mu}g$/disc, respectively. The antibacterial activity was not destroyed by heating at 80, 100, $120^{\circ}C$ for 30 min and was not affected by pH. In the inhibitory test against the Staphylococcus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction showed potent growth inhibition at the concentration of 0.1 and $0.5\;{\mu}g/mL$ for 12~24 hours and n-hexane-soluble fraction did not show any mutagenic activity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.662-670
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• 2005

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin bio-synthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) stimulates melanogenesis and enhances the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Biota Orientalis Folium on the basal melanogenic activities of B16 mouse melanoma cells, and on the ${\alpha}$-MSH or tyrosinase-induced melanogenesis. Biota Orientalis Folium alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. The decrease of cell propagation was observed in B16 cells treated with 200${\mu}$g/ml dose of Biota Orientalis Folium, indicating that Biota Orientalis Folium-induced depigmenting effect was caused by inhibition of melanin synthesis, not due to destruction of B16 cells. Pretreatment of the cells with Biota Orientalis Folium also suppressed the increase of ${\alpha}$-MSH (10 nM) induced melanin content and tyrosinase activity. Biota Orientalis Folium inhibited the revelation of ${\alpha}$-MSH induced tyrosinase protein and tyrosinase related protein and mRNA of tyrosinase in B16 melanoma cell. These results suggest that Biota Orientalis Folium inhibits melanogenesis and abrogates ${\alpha}$-MSH and tyrosinase-induced melanogenesis in B16 melanoma cells.

• Korean Journal of Environmental Agriculture
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• v.7 no.2
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• pp.111-116
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• 1988

The aquatic plants were cultivated in pots containing pig waste water, adjusted to three levels of $NH_4-N$ concentration 50, 100 and 200ppm. The aquatic plants were Eichhornia crassipes solms-laub, Monochoria korsakowii Regel et maack, Zizania caduciflora, Typha orientalis, Acorus asiaticus, Cyperus exaltaus, Colocasia antiquorum var. Yield, content and amount of nitrogen and phosphorus absorped by plants, and growth status were investigated. The results obtained are as follows. 1. The content and removal amount of nitrogen and phosphrus by plants were the highest in Eichhornia crassipes solms-laub. 2. Yield of dry matter in plants in 100ppm $NH_4-N$ was in the order of Eichhornia crassipes solms-laub>Zizania caduciflora>Typha orientalis>Monochoria korsakowii Regel et maack>Acorus asiaticus. 3. The removal amount of nitrogen by plants in the 100ppm $NH_4-N$ was in the order of Eichhornia crassipes solms-laub>Zizania caduciflora>Monochoria korsakowii Regel et maack>Typha orientalis>Acorus asiaticus>Colocasia antiquorum var>Cyperus exaltatus. Removal amount of phosphorus was in the order of Eichhornia cras sipes solms-laub>Monochoria korsakowii Regel et maack>Zizania caducilora>Typha orientalis>Acorus asiaticus> Cyperus exaltatus>Colocasia antiquorum var. 4. Concentration causing growth damage was 200ppm of $NH_4-N$ in Eichhornia crassipes solms-laub, Zizania caduciflora and Typha orientalis and 100ppm in Monochoria korsakowii Regel et maack and Acorus asiaticus. 5. Nitrogen content was the highest in leaf and phosphorus content was the highest in float of the water hyacinth. 6. The number of panicles of the water hyacinth increased by 752 pieces and dry matters were about 5,000kg/l0a during one year.

• International Journal of Advanced Culture Technology
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• v.8 no.2
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• pp.277-282
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• 2020

The first zoea of Microcassiope orientalis is described and illustrated from laboratory hatched materials. Comparisons with previous studies of the first zoeal stages in the Xanthidae from Korean waters, revealed that some former studies were incorrected. The first zoea of M. orientalis described by Ko was identical to the first zoea of Macromedaeus distinguendus described by Terada. In addition, zoeal stages of Nanocassiope granullipes described by Ko & Clark was revealed the zoeal stages of M. cassiopes. So, the first zoea of M. orientalis is redescribed and illustrated in detailed with color photo herein, and compare its morphological characteristics with previous described zoea of N. granulipes by Ko & Clark.

• Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.218-225
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• 1999

A protease without tryptic and chymotryptic activities was purified from the hepatopancreas of shrimp, Penaeus orientalis, using Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, Mono-Q, and gel chromatography. Molecular weight (M.W.) of the protease was estimated to be 27kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS­PAGE). The amino acid composition of the protease was different from that of protease from P. japonicus or trypsin from P. orientalis. The protease was completely inhibited by benzamidine, $N\alpha-p-tosyl-L-lysine$ chloromethyl ketone (TLCK), and phenylmethylsulfonyl fluoride (PMSF) and was not affected by leupeptin, pepstatin, N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), iodoacetate, and ethylenediamine tetra acetate (EDTA). The enzyme did not have any activity against Na-benzoyl-DL-arginine p-nitroanilide (BAPNA) or N-benzoyl-L-tyrosine ethyl ester (BTEE) which are specific substrates of trypsin and chymotrypsin, respectively. However, the protease showed hydrolytic activity for a carboxyl terminal of Tyr, Trp, Phe, Glu, and Cys.