• Animal Systematics, Evolution and Diversity
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• 제13권2호
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• pp.93-102
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• 1997

조간대와 수심 20m 미만의 조하대 모래틈에서 채집한 해양 완보류 2신종 - Batillipes longispinosus n. sp., B. orientalis n. sp. - 을 기재한다. 이들 2신종은 미성숙 개체와 성체 모두에서 몸통 뒷부분에 돌기가 없으며 머리에 난 여러 돌기들이 길다는 점, 제 4다리위에 긴돌기를 가진다는 점에서 특징적이다. B. longispinosus n. sp.는 Batillipes 속에 기록된 21종 중에서 B. tubernatis Pollock과 가장 유사하나 4번째 다리에 긴 돌기를 가지는 점에서 다르다. 또한 B. orientalis n. sp. 는 몸 측면 돌기를 가진다는 점에서 B. roscoffensis Kristensen와 닮았으나 머리의 모양과 4번째 다리에 긴 돌기를 가지는 점에서 뚜렷하게 구별된다.

• Animal Systematics, Evolution and Diversity
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• 제26권1호
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• pp.49-54
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• 2010

Paramolgus orientalis n. sp. is described as an associate of the antipatharian coral Antipathes japonicus from Jeju Island, Korea. The new species is distinguished from its three relatives P. constrictus, P. delicatulus, and P. insectus, all known from antipatharians in tropical waters, by its long caudal ramus which is more than four times as long as wide, by the possession of 4 setae on maxillule, and by other morphological differences in the antenna and maxilla.

• 식물분류학회지
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• 제39권2호
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• pp.107-113
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• 2009

국내에서 채집된 나자스말속(Najas L.) 식물에 대한 형태학적 식별형질을 검토한 결과, 동아나자스말 (N. orientalis)과 큰톱니나자스말(N. oguraensis)이 확인되어 국내 미기록종으로 보고한다. 동아나자스말은 원형의 엽초와 뚜렷하게 튀어나온 외종피세포벽으로서 식별할 수 있으며, 남부지역(김해, 강진)에 분포한다. 큰톱니나자스말은 톱니나자스말(N. minor)과 유사하지만 수꽃이 크고 약실이 4개인 점에서 구별되며, 남부지역(창원, 장흥)에서 확인되었다. 한편, 전국적으로 분포가 확인된 N. gracillima의 국명이 설정되어 있지 않아 실나자스말로 명명하였다. 기존에 보고된 종을 포함한 한국산 나자스말속의 여섯 종에 대한 검색표를 제시하였다.

• 환경생물
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• 제22권2호
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• pp.300-307
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• 2004

The chloroacetanilide herbicide alachlor (2-chloro-2', 6'-diethyl-N-(methoxymethyl)-acetanilide) and organochlorine insecticide endosulfan (6, 7, 8, 9, 10, 10-hexachloro-l, 5, 5a, 6, 9, 9a-hexahydro-6, 9-methano-2, 3, 4-benzodioxathiepin-3-oxide)are the highly toxic agricultural chemicals. Bombina orientalis is one of the most common amphibians in the world and comprises a large proportion of their total number. B. orientalis spawns in the farming regions at Spring when the massive application of agricultural chemicals occurs. These chemicals in farmland may threaten the reproduction of this frog. Therefore, we examined the embryotoxic and survival effects of alachlor and endosulfan at various concentrations in B. orientalis embryos. The survival rates of embryos at 312h post fertilization treated with alachlor and endosulfan were decreased with concentration dependent manner. Also, developmental malformations appeared by alachlor and endosulfan in B. orientalis embryos. The malformations showed in order of frequency with bent trunk, tail dysplasia, bent tail, thick-set body and ventral blister in alachlor treated embryos. The exposure of endosulfan produced 7 types of severe external malformations with tail dysplasia, pectoral blister, bent trunk, bent tail, cephalic dysplasia, ventral blister, and thick-set body. Following exposure to alachlor and endosulfan the types of malformations were diverse, suggesting these chemicals target multiple events in embryonic and larval development in this species. These results suggested that alachlor and endosulfan were detrimental for survival and development of B. orientalis embryos.

• 동의생리병리학회지
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• 제21권1호
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• pp.136-144
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• 2007

Biotae Orientalis has been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone and MeOH extracts of dried or fresh Biotae Orientalis, on hair growing activity of the C57BL/6N mice after topical application to skin. First, We examined on hair growth activity of extracts of Biotae Orientalis compare to control and 1% minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Biotae Orientalis to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. I fourth investigated changes of subpopulation of splenocytes and thymocytes in C57BL/6N mice for 16day using laser flow cytometry. The results were as follows : Hair growing effect of acetone and MeOH extracts of dried and fresh Biotae Orientalis was observed in 70%, 90% and 60% in hair removed skin area in 16 day respectively. Immunoreactive density of VEGF and PKC-${\alpha}$ in skin of experimental groups was weakly stained compare to control group in 10 day. Immunoreactive density of stem cell factor in skin of experimental group was heavily stained compare to control group in 10 day. Splenic TH/TC Iymphocytes of lived MeOH extracts group significantly increased compare to control group. TH cells in thymic T lymphocytes were increased compare to control group. These experiment suggest that acetone and MeOH extracts of Biotae Orientalis may be used for topical treatment of alopecia areata.

• 한국약용작물학회지
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• 제18권5호
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• pp.315-322
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• 2010

This study was carried out to investigate the antimicrobial activities of organic extracts obtained from the fruit of Thuja orientalis L. The native fruits in Korea were collected and extracted by 80% ethanol, and the extract was sequentially fractionated with n-hexane, chloroform, ethylacetate, and butanol. The fraction yields of n-hexane, chloroform, ethylacetate, butanol and water of ethanol extract were 10.15%, 10.05%, 1.45%, 45.35% and 27.55%, respectively. n-Hexane-soluble fraction showed the highest antibacterial activity against gram positive bacteria, while the chloroform, ethylacetate, butanol and aqueous fractions did not show any antibacterial activity. Minimum inhibitory concentration (MICs) on Staphyloycoccus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction were $100\;{\mu}g$, $500\;{\mu}g$ and $50\;{\mu}g$/disc, respectively. The antibacterial activity was not destroyed by heating at 80, 100, $120^{\circ}C$ for 30 min and was not affected by pH. In the inhibitory test against the Staphylococcus aureus, Bacillus subtilis and Sateptococcus pneumoniae, n-hexane-soluble fraction showed potent growth inhibition at the concentration of 0.1 and $0.5\;{\mu}g/mL$ for 12~24 hours and n-hexane-soluble fraction did not show any mutagenic activity.

• 동의생리병리학회지
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• 제19권3호
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• pp.662-670
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• 2005

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin bio-synthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) stimulates melanogenesis and enhances the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Biota Orientalis Folium on the basal melanogenic activities of B16 mouse melanoma cells, and on the ${\alpha}$-MSH or tyrosinase-induced melanogenesis. Biota Orientalis Folium alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. The decrease of cell propagation was observed in B16 cells treated with 200${\mu}$g/ml dose of Biota Orientalis Folium, indicating that Biota Orientalis Folium-induced depigmenting effect was caused by inhibition of melanin synthesis, not due to destruction of B16 cells. Pretreatment of the cells with Biota Orientalis Folium also suppressed the increase of ${\alpha}$-MSH (10 nM) induced melanin content and tyrosinase activity. Biota Orientalis Folium inhibited the revelation of ${\alpha}$-MSH induced tyrosinase protein and tyrosinase related protein and mRNA of tyrosinase in B16 melanoma cell. These results suggest that Biota Orientalis Folium inhibits melanogenesis and abrogates ${\alpha}$-MSH and tyrosinase-induced melanogenesis in B16 melanoma cells.

• 한국환경농학회지
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• 제7권2호
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• pp.111-116
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• 1988

축산폐수(畜産廢水)의 정화방법(淨化方法)의 일환으로 사각(四角) pot에 수초(水草)인 부레옥잠, 물옥잠, 줄, 부들 창포 왕골, 토란을 이식하고 관수(灌水)하면서 재배(栽培)하고 건물생산량(乾物生産量), 식물체중(植物體中)의 질소(窒素)및 인산함량(燐酸含量), 질소(窒素) 및 인산흡수량(燐酸吸收量), 부레옥잠의 생육상황(生育狀況)등을 조사(調査)하여 축산폐수(畜産廢水)의 정화능력(淨化能力)을 조사(調査)한 결과(結果)는 다음과 같다. 1 식물별(植物別) 질소(窒素), 인산함량(燐酸含量) 및 제거량(除去量)은 부레옥잠이 가장 높았다. 2. $NH_4-N$ 100ppm구(區)에서 건물수량(乾物收量)은 부레옥잠>줄>부들>물옥잠>창포의 순이었다. 3. $NH_4-N$ 100ppm구(區)에서 질소제거량(窒素除去量)은 부레옥잠>줄>물옥잠>부들>창포>토란>왕골> 순이었고, 인산(燐酸)은 부레옥잠>물옥잠>줄>부들>창포>왕골>토란순이었다. 4. 생육저해(生育沮害) $NH_4-N$ 농도(濃度)는 부레옥잠, 줄, 부들 은 200ppm이고, 물옥잠 및 창포는 100ppm이었다. 5. 부레옥잠에서 질소(窒素)는 잎에 인산(燐酸)은 부레에 많이 함유(含有)되어 있었다. 6. 부레옥잠의 년간(年間) 재배기간(栽培期間)을 7개월(個月)로보면 $NH_4-N$ 100ppm구(區)에서 분벽(分蘗)은 1개체(個體)가 752개(個)로 증가(增加)되고 건물수량(乾物收量)은 10a당(當) 5톤정도(程度)로 추정(推定)되었다.

• International Journal of Advanced Culture Technology
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• 제8권2호
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• pp.277-282
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• 2020

The first zoea of Microcassiope orientalis is described and illustrated from laboratory hatched materials. Comparisons with previous studies of the first zoeal stages in the Xanthidae from Korean waters, revealed that some former studies were incorrected. The first zoea of M. orientalis described by Ko was identical to the first zoea of Macromedaeus distinguendus described by Terada. In addition, zoeal stages of Nanocassiope granullipes described by Ko & Clark was revealed the zoeal stages of M. cassiopes. So, the first zoea of M. orientalis is redescribed and illustrated in detailed with color photo herein, and compare its morphological characteristics with previous described zoea of N. granulipes by Ko & Clark.

• Fisheries and Aquatic Sciences
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• 제2권2호
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• pp.218-225
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• 1999

A protease without tryptic and chymotryptic activities was purified from the hepatopancreas of shrimp, Penaeus orientalis, using Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, Mono-Q, and gel chromatography. Molecular weight (M.W.) of the protease was estimated to be 27kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS­PAGE). The amino acid composition of the protease was different from that of protease from P. japonicus or trypsin from P. orientalis. The protease was completely inhibited by benzamidine, $N\alpha-p-tosyl-L-lysine$ chloromethyl ketone (TLCK), and phenylmethylsulfonyl fluoride (PMSF) and was not affected by leupeptin, pepstatin, N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), iodoacetate, and ethylenediamine tetra acetate (EDTA). The enzyme did not have any activity against Na-benzoyl-DL-arginine p-nitroanilide (BAPNA) or N-benzoyl-L-tyrosine ethyl ester (BTEE) which are specific substrates of trypsin and chymotrypsin, respectively. However, the protease showed hydrolytic activity for a carboxyl terminal of Tyr, Trp, Phe, Glu, and Cys.