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High-frequency Plant Regeneration from Mature Seed-derived Callus Cultures of Orchardgrass (오차드그래스 성숙종자로부터 캘러스 유도 및 고효율 식물체 재분화)

  • Lee, Sang-Hoon;Lee, Dong-Gi;Kim, Jin-Soo;Lee, Byung-Hyun
    • Journal of Plant Biotechnology
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    • v.30 no.4
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    • pp.341-346
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    • 2003
  • In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92% when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61%. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69%. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.

Performance Analysis of Reed Solomon/Convolutional Concatenated Codes and Turbo code using Semi Random Interleaver over the Radio Communication Channel (무선통신 채널에서 RS/길쌈 연쇄부호와 세미 랜덤 인터리버를 이용한 터보코드의 성능 분석)

  • 홍성원
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.5 no.5
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    • pp.861-868
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    • 2001
  • In this paper, the performance of Reed Solomon(RS)/convolution리 concatenated codes and turbo code using semi random interleaver over the radio communication channel was analyzed. In the result, we proved that the performance of decoder was excellent as increase the interleaver size, constraint length, and iteration number. When turbo code using semi random interleaver and Hsiconvolutional concatenated codes was constant constraint length L=5, BER=10-4 , each value of $E_b/N_o$ was 4.5〔dB〕 and 2.95〔dB〕. Therefore, when the constraint length was constant, we proved that the performance of turbo code is superior to RS/Convolutional concatenated codes about 1.55〔dB〕 in the case of BER=10-4.

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Experimental and numerical investigation on exposed RCFST column-base Joint

  • Ben, Mou;Xingchen, Yan;Qiyun, Qiao;Wanqiu, Zhou
    • Steel and Composite Structures
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    • v.45 no.5
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    • pp.749-766
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    • 2022
  • This paper investigates the seismic performance of exposed RCFST column-base joints, in which the high-strength steel bars (USD 685) are set through the column and reinforced concrete foundation without any base plate and anchor bolts. Three specimens with different axial force ratios (n = 0, 0.25, and 0.5) were tested under cyclic loadings. Finite element analysis (FEA) models were validated in the basic indexes and failure mode. The hysteresis behavior of the exposed RCFST column-base joints was studied by the parametrical analysis including six parameters: width of column (D), width-thickness ratio (D/t), axial force ratio (n), shear-span ratio (L/D), steel tube strength (fy) and concrete strength (fc). The bending moment of the exposed RCFST column-base joint increased with D, fy and fc. But the D/t and L/D play a little effect on the bending capacity of the new column-base joint. Finally, the calculation formula is proposed to assess the bending moment capacities, and the accuracy and stability of the formula are verified.

Investigation of Optimal ionic Concentration of Nutrient Solution for the Water Culture of Young Welsh Onion (실파의 수경재배에 적합한 양액농도 구명)

  • Won Jae Hee;Kim Sang Soo;Jeong Byung Chan;Park Kuen Woo
    • Journal of Bio-Environment Control
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    • v.14 no.4
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    • pp.269-274
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    • 2005
  • The purpose of this experiment was to investigate optimal ionic concentration of nutrient solution for water culture of young welsh onion (Allium fistulosum). For the purpose of clarification of optimal nutrient concentration to maximize growth of young welsh onion, different nutrient concentrations of Yamazaki's solution for welsh onion seedling $(NO_3^--N\;9.0,\;NH_4^+-N\;3.0,\;PO_4^{3-}-P\;6.0,\;K^+7.0,\;Ca^{2+}\;2.0,\;Mg^{2+}\;2.0,\;and\;SO_4^{2-}-S\;4.4me{\cdot}L^{-1})$ which selected by prior experiment were treated as 0.6, 1.2, 1.8, and $2.4dS{\cdot}m^{-1}$. Increments of fresh weight, dry weight and top length were the highest in 1.2 and, in the next, were placed by the order of 1.8, 2.4, and $0.6dS{\cdot}m^{-1}$ The regression coefficients for the maximal growth of fresh weight of cv. 'Geurnjanguedaepa' and 'Tokyokuro' were $y=-42.091x^2+171.79x+11.047 (R^2=0.8946,\; R=0.9458^*)\;and\;y=-50.069x2+157.58x+15.414(R^2=0.9343,\;R=0.9692^{**})$, respectively, and optimal EC levels according to regression coefficients were 1.68 and $1.57dS{\cdot}m^{-1}$. As the conclusions, optimal nutrient levels far young welsh onion were $1.2dS{\cdot}m^{-1}$ EC in the early growth stage and $1.6\~l .7dS{\cdot}m^{-1}$ in the later growth stage.

Effect on Nutrients Removal of Algae in Aerobic High Rate Pond by Irradiance Period and pH (호기성 고율 안정조에서 빛의 조사 기간과 pH가 조류의 영양물질 제거에 미치는 영향)

  • 공석기;안승구
    • Journal of Environmental Science International
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    • v.6 no.2
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    • pp.141-152
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    • 1997
  • The pilot plant had been made so as to be an association system from the various items managed to have degrees of efficiency and It have been done to consider the experimental result with irradiance period and pH influence of all major things to treatment function of Waste Stabilization Pond. The results are as following. The attained results for continuous & cyclic irradiance 1. 24L.-reactor was prior to 12L.-12D.-reactor on oxygen generation & algal production ability. 2. 24L.-reactor was prior to 12L.-12D.-reactor on nutrients removal efficiency. 3. In 24L.-reactor it maintained 5mg/L∼6mg/L, DO concent enough to a fish's survival. The attained results for pH condition 1. Oxygen generation ^ algal production in pH 4-reactor were higher than those in pH 10-reactor. 2. The acidic condition at pH 4 and alkalic condition at pH 10 did not so much affect an algal growth and nutrients removal. The attained results for whole 1. In view of the results appeared as [(NH3-N)+(NO3-N)] removal efficiency, 89.1%∼93.9% and PO4-P removal efficiency, 34.3%∼83.7% & COD removal efficiency, 88.5%∼93.9%. It is possible to treat the wastewater with starch and pH which have been known as thedifficult problem. 2. At the point of non using methanol to nitrificate NO3-N, the nutrients removal method by using an algal growth is the most economical method in the whole nutrients removal methods. 3. The nutrients removal method by using an algal growth contributes to natural ecosystem. 4. The nutrients removal method by using an algal growth is excellant in the prevention against the eutrophication.

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NONEXISTENCE OF SOME EXTREMAL SELF-DUAL CODES

  • Han, Sun-Ghyu;Lee, June-Bok
    • Journal of the Korean Mathematical Society
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    • v.43 no.6
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    • pp.1357-1369
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    • 2006
  • It is known that if C is an [24m + 2l, 12m + l, d] selfdual binary linear code with $0{\leq}l<11,\;then\;d{\leq}4m+4$. We present a sufficient condition for the nonexistence of extremal selfdual binary linear codes with d=4m+4,l=1,2,3,5. From the sufficient condition, we calculate m's which correspond to the nonexistence of some extremal self-dual binary linear codes. In particular, we prove that there are infinitely many such m's. We also give similar results for additive self-dual codes over GF(4) of length n=6m+1.

Host Preference of Gray Field Slug, Deroceras reticulatum Müller, and Its Development and Longevity on Host Plants (작은뾰족민달팽이의 기주 선호성 및 기주에 따른 발육과 수명)

  • Kim, Hyun-Ju;Bae, Soon-Do;Yoon, Young-Nam;Choi, Byeong-Ryeol;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.51 no.1
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    • pp.33-37
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    • 2012
  • This study was conducted to investigate food preference and developmental characteristics of the gray field slug, $Deroceras$ $reticulatum$ M$\ddot{u}$ller (Stylommatophora: Limacidae) using various foods. The food preference of $D.$ $reticulatum$ was most highest on Chinese cabbage ($Brassica$ $campestris$ L., followed on cucumber ($Cucumis$ $sativus$ L.), and significantly low on beet ($Beta$ $vulgaris$ var. Ruba), kale($Brassica$ $oleracea$ L. var. $acephala$ D.C), carrot (Daucus carota L.) and Chinese cabbage ($Brassica$ $campestris$ L.) which were not different significantly. The total number of eggs laid by $D.$ $reticulatum$ was the highest (n = 109.6) on cucumber and the lowest (n = 10.1) on leaf perilla ($Perilla$ $frutescens$ var. $japonica$ Hara). Hatchability was the highest (92.4%) on cucumber and the lowest (62.5%) on leaf perilla. The egg developmental period was not significantly different from 12 days to 13 days among host plants. Juvenile period and adult longevity ranged from 75 days to 111 days and 66 days to 187 days, respectively. Thereby, life span from egg to adult longevity of $D.$ $reticulatum$ was the longest on cucumber with 273 days and the shortest on 190 days on leaf perilla.

Operation Parameters on Biological Advanced Treatment of Phenolic High-Strength Wastewater (페놀계 고농도 유기성 폐수의 생물학적 고도처리 운전인자)

  • Hong, Sung-Dong;Park, Chul-Hwi
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.4
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    • pp.797-806
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    • 2000
  • The objectives were to compare the biodegradable threshold concentrations of phenol with the different composition of the influent carbon source and examine the SMA (Specific Methanogenic Activity)and the possibility of simultaneous removal of high-strength organics and nitrogen compounds in UASB(Upflow Anaerobic Sludge Blanket) - PBR(Packed Bed Reactor) process. The results showed that UASB reactors were efficient to remove phenol and phenol + glucose from synthetic wastewater. At phenol conc, of 600 mg/L and SCOD conc. of 2100 mg/L in UASB reactor(with only phenol as substrate), the removal efficiencies of phenol and SCOD were over 99% and 93% respectively, under MLVSS of 20 g. The activity of microorganism was $0.112g\;phenol/g\;VSS{\cdot}d$, $0.351g\;SCOD/g\;VSS{\cdot}d$. The gas production rate was $0.115L/g\;VSS{\cdot}d$ and $CH_4$ content in gas was about 70%. At phenol conc. of 760 mg/L and SCOD conc. of 4300 mg/L in UASB reactor( with phenol + glucose as substrates), the removal efficiencies of phenol and of SCOD were over 99% and 90% respectively, under MLVSS of 20 g. The activity of microoganism was $0.135g\;phenol/g\;VSS{\cdot}d$, $0.696g\;SCOD/g\;VSS{\cdot}d$. The gas production rate was $0.257L/g\;VSS{\cdot}d$ and $CH_4$ content in gas was about 70%. Serum bottle test showed that the activity of granule was inhibited over 1600 mg/L phenol conc, and denitrification and methanogenesis simultaneously took place in UASB granules under co-substrates conditions. PBR reactor packed with cilium type media, was efficient in nitrification. In condition of $0.038kg\;NH_4-N/m^3-media{\cdot}d$. 10~12 mg/L phenol conc. and 200~500 mg/L SCOD conc., nitrification efficiency was over 90% and phenol removal efficiency was over 98%.

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Cloning and Expression of the dapD Gene from Brevibacterium lactofermentum in E. coli (Brevibacterium lactofermentum의 dapD 유전자의 Cloning 및 E. coli에서의 발현)

  • 김옥미;박선희;박혜경;이승언;하대중;이갑랑
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.802-805
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    • 2001
  • The dapD gene of Brevibacterium lactofermentum encoding tetrahydrodipicolinate N-succinyl transferase, one of the enzymes involved in lysine biosynthesis, was cloned by complementation of Escherichia coli dapD mutnat. The recombinant plasmid pLS1 was found to contain a 3.6 kb DNA fragment. Southern hybridization analysis confirmed that the cloned DNA fragment originated from B. lactofermentum. The data of L-lysine production showed that the B. lactofermentum dapD gene was expressed in E. coli.

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Buffering effects of shock loads in sequencing batch reactor (SBR에서 충격부하에 따른 완충효과)

  • Ji, Dae-Hyun;Lee, Kwang-Ho;Lee, Jae-Kune
    • Journal of Korean Society of Water and Wastewater
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    • v.22 no.5
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    • pp.581-587
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    • 2008
  • In this study, we investigated the buffering effect with different COD, $NH_4{^+}-N$ and TP shock loads on the performance of lab-scale Sequencing Batch Reactor(SBR) using synthetic wastewater. This study was operated under the following conditions : HRT, 12 hrs : MLSS, 2,000 mg/L : F/M ratio, $0.2kgCOD/kgMLSS{\cdot}d$ : SRT, 20days, and was increased by a factor, COD : ranging from 200-2000 mg/L, $NH_4{^+}-N$ : ranging from 30-300 mg/L, T-P : ranging from 5-50 mg/L in the reactor. As results, COD removal rate at different shock loads was decreased until 42.1%(stable state : 95%) and concentration with effluent was higher than 695 mg/L(at Run 6). In case of $NH_4{^+}-N$ removal rate was decreased until 35.8% from 97.0% and also T-P removal rate was decreased until 5.0%. Effluent concentrations of COD, $NH_4{^+}-N$ and T-P were rapidly increased according to shock loads and needed 3, 4 and 6 additional cycle times for recovering the stable condition.