• 한국작물학회지
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• 제47권2호
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• pp.108-115
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• 2002

The effects of long-term fertilization on soil properties and nutrient availability are not well documented for cassava cultivation in Vietnam. In 1990, a field research plots were established with 12 treatments to test the effect of different rates of nitrogen (N), phosphorus (P) and potassium (K) on soil properties in Acrisols at Thai Nguyen University in Northern Vietnam. In 1999, composite soil samples (0 to 20cm depth) were collected from eight selected plots for measurements of nutrient supply rates by ion exchange membrane probes and for growing corn and canola in a growth chamber with and without added lime. Generally, long-term nitrogen (N) fertilization increased available N supply rates but decreased available potassium (K) and magnesium (Mg). Long-term phosphorus(P) applications increased canola N, calcium (Ca) and Mg uptake. Canola P uptake increased with increased P rates only when lime was added. Long-term K applications increased canola N, K, Ca, Mg uptake but only significantly increased corn N uptake. Liming significantly increased uptake of N, P, K, Ca, Mg and S for both corn and canola. However, N $H_{4-}$N, K and Mg soil supply rates were reduced when lime was added, due to competition between Ca from the added lime and other nutrients.

• 한국발생생물학회지:발생과생식
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• 제24권4호
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• pp.297-306
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• 2020

Repetitive changes in the intracellular calcium concentration ([Ca²⁺]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca²⁺]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca²⁺ ion elevation during [Ca²⁺]i oscillations are Ca²⁺ release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca²⁺ ion influx through Ca²⁺ channel on the plasma membrane. Ca²⁺ channels have been characterized into voltage-dependent Ca²⁺ channels (VDCCs), ligand-gated Ca²⁺ channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca²⁺]i oscillation was observed in a Ca²⁺ contained medium with sperm factor or adenophostin A injection but disappeared in Ca²⁺ free medium. Ca²⁺ influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca²⁺]i oscillation profiles in SrCl₂ treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca²⁺ influx is essential for [Ca²⁺]i oscillation during mammalian fertilization. This Ca²⁺ influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.450-452
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• 2004

Using factor analysis and bivariate comparisons of major components in ground water, three geochemical processes were identified as controlling factors of ground water chemistry; 1) natural mineralization by water rock interactions, 2) effect of seawater which includes salinization by seawater near seashores and deposition of sea salt, and 3) nitrate contamination by N fertilization. Contribution of rainfall was also estimated from the measured composition of wet deposition. The geochemical processes were separated using total alkalinity as an indicator for natural mineralization, Cl for effect of seawater, and nitrate for N fertilization. Relatively high correlation of major components with nitrate suggests that nitrification of nitrogenous fertilizers significantly affects ground water chemistry. Total cations derived from nitrate sources have good linearity for nitrate in equivalent basis with a slope of 1.8, which is a mean of proton production coefficients in nitrification of two major compounds in nitrogenous fertilizers, ammonium and urea. Contribution of nitrate sources to base cations, Cl, and SO$_4$ in ground water was determined considering maximum contribution of natural mineralization to estimate a threshold of the effect of N fertilization for ground water chemistry, which shows W fertilization has a greatest effect than any other processes in ground water with nitrate concentration greater than 50 mg/L for Ca, Mg, Na and with concentration greater than 30 mg/L for Cl and SO$_4$.

• 한국초지조사료학회지
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• 제13권2호
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• pp.93-98
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• 1993

To determine the effects of potassium($K_2O$) fertilization level(0, 100, 200 and 300 kg/ha/year) on the growth, dry matter(DM) yield, nutritive value and nitrate nitrogen($NO_3$-N) concentration of grasses grown under shading condition, this experiment was carried out in Grassland Div., LES, 1989. The plants were sampled on different growing seasons (spring, summer and autumn) and growth stages (grazing and soiling), respectively. Shade was controlled artificially ca. 45-50%, and the level of nitrogen fertilization was 200 kg/ha/year in all treatments. Higher DM was produced in spring 1, 453 kg at grazing and 2, 364 kg/ha at soiling stage, and DM production was increased with increasing level of $K_2O$. In this experiment, the optimum amount of $K_2O$ fertilizer was 200 kg/ha. The neutral detergent fiber, acid detergent fiber, DM digestibility, and relative feed value of grasses were not affected by $K_2O$ fertilization, regardless of growing season and growth stage. The $NO_3$-N concentration of grasses grown in spring was very low (ca. 1.0%), regardless of growth stage and $K_2O$ level. However, $NO_3$-N concentration was very high in summer and autumn season, also $NO_3$-N was decreased significantly with increasing level of $K_2O$ fertilization (P<0.05). Application of $K_2O$ fertilizer, therefore, is thoughs to be desirable for reducing $NO_3$-N concentration of grasses, especially in summer and autumn season. So annual split fertilization of $K_2O$ could be recommended on woodland pasture.

• 한국환경농학회지
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• 제18권4호
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• pp.304-309
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• 1999

소나무, 일본잎갈나무, 자작나무 묘목을 대상으로 온실 내에서 질소와 인 비료를 사용하고, 묘목 생체량, 잎, 줄기, 뿌리 등의 부위별 양분 농도, 그리고 양분이용효율에 미치는 영향을 연구하였다. 대부분의 경우 시비가 묘목의 생체량 변화에 미치는 효과는 없는 것으로 나타났다. 그러나 시비 후 묘목 내 부위별 질소와 인 농도는 증가하였다. 이러한 결과는 시비가 묘목을 양분 과다 이용상태에 이르게 한 것으로 사료되었다. 양분이용효율은 지상부와 지하부 생체량에 대한 묘목 내 양분 함량의 비로 계산하였는데, 시비 후 질소와 인의 이용효율은 소나무와 자작나무에서 감소하였으나, 일본 잎갈나무에서는 변화를 보이지 않았다. 수종별 양분이용효율은 자작나무에서 가장 높고, 소나무와 일본잎갈나무의 순으로 점차 감소하는 것으로 나타났다.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2000년도 학술발표회 발표논문집
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• pp.623-628
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• 2000

This study were conducted to evaluate the mass balance of phosphorus and nitrogen with cropping in experimental pot where the mass of input concentration, and fertilization were variable. Four treatments include CSWNF, TWCF, SWNF. And these cases were compared to the control case of tap water irrigation with conventional fertilization (CONTROL). Fertilization were following conventional fertilization , N : P : K = 11kg : 7kg : 8kg. Input water loading was CSWNF (N:48.7g ,P:3.6g), TWCF(7.8g, 0.6g), SWHF(38.8g ,2.9g), TWNF(38.8g, 2.9g ) and CONTROL(0g ,0g) The result is nitrogen decrease rate; TWCF(19.2%), SWHF(14.9%), CSWNF(9.2%) and SWCF(5.6%). phosphorous decrease rate ; TWCF (10%), SWHF(3.7%), SWCF(0.9%) and CSWNF(0.3%).

• Clinical and Experimental Reproductive Medicine
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• 제16권2호
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• pp.153-160
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• 1989

The effect of antisperm antibodies (ASA) on the human in vitro fertilization (lVF) process was evaluated by analyzing the IVF data between October and December 1988 at Seoul National University Hospital prospectively. The immunobead test (IBT) was used to identify Ig G, Ig A, and Ig M in the serum, semen, and follicular fluid from 93 couples undergoing in vitro fertilization-embryo transfer (lVF-ET ) . The fertilization rate in couples with ASA to sperm head of at least one isotype in female serum (n= 10) was significantly less than that in couples without ASA to sperm head (n=83; 28.5% versus 45.3% , p=0.028). The presence of ASA to sperm head in follicular fluid (n=8) also reduced fertilization rate from 45.3% to 24.4% (p=O.0l3). However, ASA binding to sperm head in male serum and semen did not predict fertilization. Similarly, ASA binding to sperm tail and tail-tip did not reduced the oocyte fertilization rate significantly in any of the fluids tested. The zygote cleavage rate was not reduced in the presence of ASA. These results suggest that the presence of ASA to sperm head in female serum and follicular fluid is associated with reduced fertilization in IVF-ET. Another observation is that the oocyte that do fertilize in the presence of antisperm antibodies can subsequently proceed with normal cleavage. The results of this investigation therefore suggest that the IBT is a useful test forscreening of women participat.ing IVF-ET program.

• 한국초지조사료학회지
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• 제17권4호
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• pp.407-414
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• 1997

In this study, the optimum cutting 6equency and level of mineral nitrogen fertilization were investigated for the production of Reed canarygrass in uncultivated rice paddy. The results are summarized as follows; 1. Higher relative dry matter yields were recorded in 2nd cut of plots with 3 and 5 cutting frequencies, and 3rd cut of plot with 4 cutting kquency, respectively. 2. With no nitrogen fertilization, mean dry matter yields per year were 6.2~7.6 tonsha and the highest yield appeared in plot with 4 cutting 6equency. 3. The increased fertilization of mineral nitrogen resulted in the increased dry matter yield. Significantly higher dry matter yields than that of no nitrogen fertilization were recorded in fertilization of 90 kg nitrogen per year in 3 cutting fequency, 240 kg in 4 cutting frequency and 150 kg in 5 cutting 6equency respectively. 4. Efficiency of dry matter production with nitrogen fertilization(kg DMkg N) was higher in 30kg Nhalcut in 3 and 5 cutting eequency, 60kg Nhalcut in 4 cutting 6equency respectively. In each cutting kequency, the higher efficiency of dry matter production appeared in 1st cut in 3 cutting kequency, and 2nd cut in 4 and 5 cutting 6equency respectively. 5. Economic N level(kg/ha) was 179.5~242.3kg/ha in 3 cutting 6equency, and 189.6-241.6kg/ha and 167.0 ~253.2kg/ha in 4 and 5 cutting 6equency respectively, and marginal dry matter yields were 11.4~ 12.3 tons/ ha, 11.2 ~ 11.8 tons/ha and 8.3 ~9.1 tons/ha in 3, 4 and 5 cutting 6equency respectively. 6. The limiting N fertilization level to the highest dry matter were estimated to be 569.9kg/ha, 492.4kg/ha and 654.lkg/ha in 3, 4 and 5 cutting kequency respectively.

• 한국초지조사료학회지
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• 제8권1호
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• pp.33-39
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• 1988

Insufficient herbage during the fall in the temperate areas of the world has been a serious limitation in animal production, but leafy brassicae are potentially very useful for extending the grazing season when the growth and quality of grass is poor. This study was conducted to determine the effects of cutting stage and N fertilization on dry matter (DM) yield, forage quality and chemical composition of forage rape (Brassica napus Subsp. oleifera). The experiment was conducted in the Experimental Livestock Farm of Seoul Nat'l Univ., at Suweon, during 1985- 1986. The results obtained are summarized as follows: 1. DM percentage and yield significantly increased as the growth period was prolonged and decreased as the N fertilization increased, but in-vitro dry matter digestibility (IVDMD) was unaffected by the treatments. 2. Plant height, DM yield and $NO_3$-N content significantly increased as the rate of N fertilization increased and DM percentage decreased, but the IVDMD was unaffected. 3. The forage rape had high percentage of crude protein and ash. Glutamic acid, pro!ine and aspartic acid were the major amino acids. Concentration of P, Ca and Mg were high.

• 한국연초학회지
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• 제4권1호
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• pp.51-59
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• 1982

This experiment was conducted to investigate the effects of fertilization level, additional dressing time and form of nitrogen on the growth and formation of Alkaloid in flue-cured tobacco. The results are abstracted as follows. Length and width of harvested leaves, area of a leaf, leaf area per plant, number of harvested leaves, weight of a leaf, and leaf weight of a plant were larger in the $NO_3$-N plot than $NH_4$-N plot. Length, width and area of the middle leaves increased by additional dressing on 7 days before topping, but the dry weight Per unit leaf area of those was decreased. Leaf shape was broaden in additional dressing plots in comparison with 100% of basal dressing plot, but there is no difference among the additional dressing plots. And the smallest leaf shape index was shown at upper leaves in 100% of basal dressing Plot. Leaf weight of a Plaint was the highest in 12kg/10a Plot among $NO_3$-N form and in 12kg/10a plot among $NH_4$-N form. Total Alkaloid content was higher in $NH_4$-N plot between fertilization forms, and highest in 15kg/10a plot among fertilization levels and at the position of middle and upper leaves in the plot of 7 days before topping among additional dressing times.