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Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism

  • Manalaysay, Jessica G.;Antonio, Nathaniel D.;Apilado, Ralph Lorenz R.;Bambico, Joseph F.;Mingala, Claro N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.2
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    • pp.262-266
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    • 2017
  • Objective: This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods: Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length polymorphism, amplifying the 416 bp region of the BAX gene that was subjected to digestion using the Ear I enzyme. Sequencing was also conducted to validate the results. Results: Results revealed that out of 538 samples tested, 411 (76.4%) of the samples were found to be normal whereas the remaining were carriers of the mutation in which 80 (14.9%) were heterozygous mutants and 47 (8.7%) were homozygous mutants. Pietrain breed was found to have the highest incidence. Conclusion: Having a scrotal hernia eliminates the chances of using the boar as a breeder stock because the following generations arising from it would most likely exhibit herniation. It is therefore advised to establish a genetic screening method for Scrotal Hernia in the Philippines to eliminate the negative gene from the herd.

Assessment of Malignancy in Brain Tumors by 3T MR Spectroscopy

  • 최보영;전신수;이재문;정성택;안창범;오창현;김선일;이형구;서태석
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2003.09a
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    • pp.76-76
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    • 2003
  • Purpose: To assess clinical proton MR spectroscopy (MRS) as a noninvasive method for evaluating tumor malignancy at 3T high field system. Methods: Using 3T MRI/MRS system, localized water-suppressed single-voxel technique in patients with brain tumors was employed to evaluate spectra with peaks of N-acetyl aspartate (NAA), choline-containing compounds (Cho), creatine/phosphocreatine (Cr) and lactate. On the basis of Cr, these peak areas were quantificated as a relative ratio. Results: The variation of metabolites measurements of the designated region in 10 normal volunteers was less than 10%. Normal ranges of NAA/Cr and Cho/Cr ratios were 1.67$\pm$018 and 1.16:1:0.15, respectively. NAA/Cr ratio of all tumor tissues was significantly lower than that of the normal tissues (P=0.005). Cho/Cr ratio of high-grade gliomas was significantly higher than that of low-grade gliomas (P= 0.001), Except 4 menigiomas, lactate signal was observed in all tumor cases. Conclusions: The present study demonstrated that the neuronal degradation or loss was observed in all tumor tissues. Higher grade of brain tumors was correlated with higher Cho/Cr ratio, indicating a significant dependence of Cho levels on malignancy of gliomas. This results suggest that clinical proton MR spectroscopy could be useful to predict tumor malignancy. Acknowledgement: This study was supported by a grant of the Mid and Long Term Nuclear R/D Plan Program, Ministry of Science and Technology, Republic of Korea.

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Real-time Robotic Vision Control Scheme Using Optimal Weighting Matrix for Slender Bar Placement Task (얇은 막대 배치작업을 위한 최적의 가중치 행렬을 사용한 실시간 로봇 비젼 제어기법)

  • Jang, Min Woo;Kim, Jae Myung;Jang, Wan Shik
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.26 no.1
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    • pp.50-58
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    • 2017
  • This paper proposes a real-time robotic vision control scheme using the weighting matrix to efficiently process the vision data obtained during robotic movement to a target. This scheme is based on the vision system model that can actively control the camera parameter and robotic position change over previous studies. The vision control algorithm involves parameter estimation, joint angle estimation, and weighting matrix models. To demonstrate the effectiveness of the proposed control scheme, this study is divided into two parts: not applying the weighting matrix and applying the weighting matrix to the vision data obtained while the camera is moving towards the target. Finally, the position accuracy of the two cases is compared by performing the slender bar placement task experimentally.

The Comparative Study based on Gompertz Software Reliability Model of Shape Parameter (곰페르츠형 형상모수에 근거한 소프트웨어 신뢰성모형에 대한 비교연구)

  • Shin, Hyun Cheul;Kim, Hee Cheul
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.10 no.2
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    • pp.29-36
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    • 2014
  • Finite failure NHPP software reliability models presented in the literature exhibit either constant, monotonic increasing or monotonic decreasing failure occurrence rates per fault. In this paper, proposes the Gompertz distribution reliability model, which made out efficiency application for software reliability. Algorithm to estimate the parameters used to maximum likelihood estimator and bisection method, model selection based on mean square error (MSE) and coefficient of determination$(R^2)$, for the sake of efficient model, was employed. Analysis of failure using real data set for the sake of proposing fixed shape parameter of the Gompertz distribution was employed. This analysis of failure data compared with the Gompertz distribution model of shape parameter. In order to insurance for the reliability of data, Laplace trend test was employed. In this study, the proposed Gompertz model is more efficient in terms of reliability in this area. Thus, Gompertz model can also be used as an alternative model. From this paper, software developers have to consider the growth model by prior knowledge of the software to identify failure modes which can was helped.

A Study of the Relationship Analysis of Power Conversion and Changed Capacitance in the Depletion Region of Silicon Solar Cell

  • Kim, Do-Kyeong;Oh, Yeong-Jun;Kim, Sang-Hyun;Hong, Kyeong-Jin;Jung, Haeng-Yeon;Kim, Hoy-Jin;Jeon, Myeong-Seok
    • Transactions on Electrical and Electronic Materials
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    • v.14 no.4
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    • pp.177-181
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    • 2013
  • In this paper, silicon solar cells are analyzed regarding power conversion efficiency by changed capacitance in the depletion region. For the capacitance control in the depletion region of silicon solar cell was applied for 10, 20, 40, 80, 160 and 320 Hz frequency band character and alternating current(AC) voltage with square wave of 0.2~1.4 V. Academically, symmetry formation of positive and negative change of the p-n junction is similar to the physical effect of capacitance. According to the experiment result, because input of square wave with alternating current(AC) voltage could be observed to changed capacitance effect by indirectly method through non-linear power conversion (Voltage-Current) output. In addition, when input alternating current(AC) voltage in the silicon solar cell, changed capacitance of depletion region with the forward bias condition and reverse bias condition gave a direct effect to the charge mobility.

Piezoelectric and Dielectric Properties of PMN-PNN-PZT Ceramics for Ultrasonic Generator with Calcination Temperature (하소온도변화에 따른 초음파절삭기용 PMN-PNN-PZT 세라믹스의 압전 및 유전 특성)

  • Yoo, Ju-Hyun;Kim, Seung-Won;Seo, Dong-Hi;Lee, Eun-Sup;Choi, Nak-Gu;Jeong, Hoy-Seung
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.30 no.3
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    • pp.152-156
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    • 2017
  • In this paper, $Pb(Mn_{1/3}Nb_{2/3})_{0.07}(Ni_{1/3}Nb_{2/3})_{0.10}(Zr_{0.5}Ti_{0.5})_{0.83}O_3$ ceramics were fabricated by the conventional solid state method to obtain excellent dielectric properties for ultrasonic generators. The effects of 2nd calcination temperature on their microstructure and piezoelectric properties were systematically investigated. The tetragonality increased in the ceramics when 2nd calcination temperature increased to the optimized temperature at $750^{\circ}C$. At that temperature, excellent physical properties ($d_{33}=352\;pC/N$, ${\varepsilon}_r=1,687$, $k_p=0.570$, $Q_m=1,640$) were obtained for ultrasonic generator application.

Genetic Improvement of Some Traits in Four Strains of Silkworm, Bombyx mori L.

  • Moghaddam S. H. Hosseini;Jomeh K. N. Emam;Mirhosseini S. Z.;Gholamy M. R.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.2
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    • pp.95-99
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    • 2005
  • A breeding plan was carried out on four commercial strains of silkworm (Bombyx mori L.) 101, 102, 103 and 104 to improve some important traits. Genetic gain or response to selection $({\Delta}G)$, heritability of cocoon shell weight (CSW) and specific combining ability effects were estimated to determine the strains that can be improved. Strain 101 had lowest heritabitity, ${\Delta}G$ and viability. Strain 102 was acceptable in selection response but its viability was low. Therefore these two strains were not suitable for more selection. As a result, only lines 103 and 104 were chosen for further improvement. Intra population selection based on independent culling level method practiced from third to sixth generation for both productive and viability traits simultaneously. While CSW and CW had increasingly enhanced during primary generations, they went slightly up after third generation. According to negative genetic correlation, viability decreased during primary generations, but after third generation that paid attention to balanced development of both productive and viability traits, viability increased so that the pupation rate reached to $91\%$ in 103 and $97\%$ in 104 for last generation $(G_8)$.

Experimental investigations on the structural behaviour of a distressed bridge

  • Dar, M.A.;Subramanian, N.;Dar, A.R.;Raju, J.
    • Structural Engineering and Mechanics
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    • v.56 no.4
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    • pp.695-705
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    • 2015
  • Distressed structures require necessary remedial measures in order to restore their original structural properties like strength and stiffness. Validating the effectiveness of the proposed qualitative remedial measure experimentally is of utmost importance as there is no well-established analytical method to verify the effectiveness of the same quantitatively. Prototype testing which would have been the best option for this purpose would not only prove costly but also be associated with numerous practical difficulties; hence model testing is resorted as the only option for the purpose. This paper presents one such typical experimental study on the structural behavior of a distressed bridge, mainly observed in the form of prominent tilt in the bearing plate in transverse and longitudinal direction on downstream side. The main focus of the proposed experimental investigation is to assess the structural behavior particularly the load carrying capacity. The extent of deformation of some models with specific structural arrangements and some models with specific need based remedial measures were also studied. This study also assessed the contribution of each remedial measure towards restoration individually and collectively.

Some precautions to consider in using wavelet transformation for damage detection analysis of plates

  • Beheshti-Aval, S.B.;Taherinasab, M.;Noori, M.
    • Smart Structures and Systems
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    • v.11 no.1
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    • pp.35-51
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    • 2013
  • Over the last two decades Wavelet Transformation (WT) method has been widely utilized for the damage identification of structures. The main objective of this paper is to discuss and present some of common shortcomings and limitations of mathematical software, as well as other precautionary measures that need to be considered when using them for wavelet analysis applications. Due to popular usage of MATLABMATLAB(R) comparing to other mathematical tools among researchers for data processing of structural responses through WT analysis, this software was chosen for specific study. To the best of the authors' knowledge, these limitations and observations have not been previously identified or discussed in the literature. In this work, a square plate with a severe damage, in form of a crack, parallel to the left edge of the plate is selected for a pilot study. The steady state harmonic response is used for measuring the deflection shape across the line parallel to one edge and perpendicular to the damage. Several criteria and cases such as the smallest size damage that can be detected, correlation between the crack width and the number of sampling points, and the influence of the damage thickness on the accuracy of the result are investigated.

Factors Relating to Social Physique Anxiety in Adolescent Girls (청소년기 여학생의 사회적 체격불안 관련 요인)

  • Lee, Eun-Joo
    • Women's Health Nursing
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    • v.14 no.2
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    • pp.93-103
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    • 2008
  • Purpose: This descriptive study was to explore the factors relating to Social Physique Anxiety(SPA) in the adolescent girls(N=700). These factors included the biological(level of school[age], period of menstruation, and BMI), the sociocultural(Influence of Mass Media, [IMM], Stereotype of Ideal Female Body[SIFB], type of school, perceived figure, and ideal figure) and the BW control-related factors(experience of bodyweight [BW] control, satisfaction at the result of BW control, and motive of BW control). Method: The sample was 700 girls of the middle and high schools in Jeju-do. The data was collected by the self-reported questionnaire and processed with the SPSS Win 12.0 program. Result: 26% of the variance in SPA was explained by the biological factors(level of school) and the sociocultural factors (perceived figure, ideal figure, type of school, and IMM). SPA, IMM, and SIFB were significantly correlated with each others(r=1,65-2.92, p<.05). The mean SPA scores according to BW control-related factors were significantly different. Conclusion: This results suggest that media education is necessary to reduce or eliminate the adolescent girl's SPA. In addition, SPA needs to be considered as the important concept for the future nursing researches and interventions related to BW control.

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