• The Korean Journal of Mycology
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• v.46 no.3
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• pp.333-344
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• 2018

Selection of the optimal culture medium and evaluation of the antimicrobial activity against various phytopathogens were performed for four entomopathogenic fungal isolates with excellent insecticidal and antimicrobial activity against the two-spotted spider mite (Tetranychus urticae), green peach aphid (Myzus persicae), and gray mold (Botrytis cinerea). The optimal medium was selected by measuring the amount of blastospore production and the antifungal activity of the culture medium. On the basis of these experiments, GY medium was selected for Beauveria bassiana 2R-3-3-1 and Metarhizium anisopliae 4-2, SD3, and PDB medium for B. bassiana SD15. The antimicrobial activity test against other phytopathogens indicated that all four isolates showed high antifungal activities against Colletotrichum acutatum and Sclerotinia sclerotiorum. However, for Phytophthora capsici and C. fructicola, only M. anisopliae SD3 showed a high antifungal activity against P. capsici, and the other three isolates had little activity. Antibacterial activity against Clavibacter michiganensis subsp. michiganensis was high in two isolates of M. anisopliae but not in two isolates of B. bassiana. Thus, it was confirmed that entomopathogenic fungi effective for pest control could be effectively used as a control agent for various plant diseases.

• The Korean Journal of Pesticide Science
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• v.5 no.2
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• pp.18-25
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• 2001

Ethanol extracts from 46 vegetables were tested their insecticidal activities toward five agricultural insect pests and four stored-product insect pests. The efficacy varied with both agricultural insects/stored-product insects and vegetable species used. Potent insecticidal activities, at the concentration of 5,000 ppm, were produced from extracts of Nelumbo nucifera and Ulva lactuca against Myzus persicae, Zea ways and Z. mays (leaf) against Nilaparvata lugens, Citrullus vulgaris (seed) and U. lactuca against Plutella xylostella, N. nucifera, Z. mays, and Z. mays (leaf) against Spodoptera litura, and C. vulgaris (seed), Daucus carota, Helianthus annuus (leaf), H. annuus (flower), Lactuca sativa, and Zingiber officinale against Tetranychus urticae. Potent insecticidal activities at the concentration of 2,500 ppm were exhibited from the extracts of N. nucifera and U. lactuca against M. persicae, Z. mays against N. lugens, C. vulgaris (seed) and U. lactuca against xylostella, N. nucifera and Z. mays against S. litura, and C. vulgaris (seed), H. annuus (flower), and L. santiva against T. urticae. Against four stored-product insect pests at 50 ppm, extracts of C. vulgaris (seed) and Cucurbita moschatla (seed) against Sitophilus oryzae and C. vulgaris (seed), H. annuus (seed), and Z. officinale against Plodia interpunctellfa revealed potent insecticidal activities over 80% mortality. In tests with Callosobruchus chininsis and Lasioderma serricorne, extracts of all vegetables tested exhibited meager and no activity.