• 제목/요약/키워드: Myristica fragrans Houtt

검색결과 9건 처리시간 0.024초

육두구(Myristica fragrans Houtt)로부터 Phenylpropanoid의 분리 (Phenylpropanoids from Myristica fragrans Houtt)

  • 송명종;안은미;방면호;김세영;노영덕;권병목;이현선;백남인
    • Applied Biological Chemistry
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    • 제47권3호
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    • pp.366-369
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    • 2004
  • Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated sing $CHCl_3$, EtOAc, n-BuOH and water, successively. The n-BuOH fraction gave three phenylpropanoids through application of silica gel column chromatographies. The chemical structures of the phenylpropanoids were determined by the interpretation of several spectral data, including NMR and MS as meso-dihydroguaiaretic acid (1), nectandrin B (2) and syringin methyl ether (3). Compound 1, which was first isolated from this plant by authors, showed inhibitory activities with $60.0{\pm}2.1%\;(100\;{\mu}g/ml),\;42.6{\pm}0.9%\;(140\;{\mu}g/ml)\;and\;12.2{\pm}0.2%\;(200\;{\mu}g/ml)$ on ACAT(acyl-CoA:Cholesterol Acyltransferase), chitin synthase III and HMG-CoA reductase (3-hydroxy-3-methylglutaryl coenzyme A reductase), respectively. Compound 3 showed inhibitory activities with $27.2{\pm}0.9%\;(100\;{\mu}g/ml),\;45.5{\pm}0.8%\;(200\;{\mu}g/ml)$ on ACAT and chitin synthase III.

Traditional Unani uses with multiple pharmacological activities of aril of Myristica fragrans (Mace)

  • Sultana, Arshiya;Najeeya, Abdul G.F.;Anjum, Amera
    • 셀메드
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    • 제8권2호
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    • pp.6.1-6.7
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    • 2018
  • Myristica fragrans Houtt is commonly known as "nutmeg", it produces two spices: mace and nutmeg. Mace and nutmeg are strongly aromatic in nature and known as jowzabuwa and javetri/bisbasah respectively in the Unani system of medicine. M. fragrans was used as early as 700 BCE by Indian, however, ancient Greeks and Romans were not aware of it. Later Arab traders introduce M. fragrans into Europe followed by Portuguese and Dutch merchants. Mace is very useful medicine in the Unani system of medicine because of its therapeutic uses in salasal al-bawl (urinary incontinence), amrad-i-qalb (cardiac diseases), amrad-i-dimagh (central nervous system), zo'fe bah (sexual debility), amrad al-rahim (uterine diseases), and su-i-hazim (indigestion). The most important constituents of mace essential oil are ${\alpha}-pinene$, sabinene, myrcene, limonene, 1,8-cineole, terpinen-4-ol, myristicin, ${\gamma}-terpinene$, and safrole. The seed and mace extract of nutmeg contain quite high tannins, flavonoids, and terpenoids. Mace has pharmacological functions such as antibacterial and antifungal, anti-inflammatory, analgesic, antidiarrhea, antioxidant, chemoprotective, neuropharmacologic, and antidiabetic properties. To explore the correlation between the traditional uses and the same proven by recent researches, a comprehensive review is highlighted in this paper. Further, pharmacological activities which are not reported in classical texts are also discussed.

Tyrosinase Inhibitory Activities of Safrole from Myristica fragrans Houtt.

  • Cho, Soo Jeong;Kwon, Hyun Sook
    • Journal of Applied Biological Chemistry
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    • 제58권4호
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    • pp.295-301
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    • 2015
  • Five phenylpropanoids (1-5), a benzofuran neolignan (6), two 8-O-4'-neolignans (7-8), and five tetrahydrofuran lignans (9-13) were isolated from a methanol extract of Myristica fragrans seeds. The structures of 1-13 were determined by $^1H$- and $^{13}C$-NMR spectroscopic data analyses and a comparison with the literature data. Compound 3 was isolated for the first time from this plant. All the isolated compounds were evaluated for their inhibitory activity against tyrosinase. Among them, safrole (1) showed significant inhibitions against both the monophenolase ($IC_{50}=32.11{\mu}M$) and diphenolase ($IC_{50}=27.32{\mu}M$) activities of tyrosinase. The kinetic analysis shows that safrole (1) is competitive inhibitors for both monophenolase and diphenolase. The apparent inhibition constant ($K_i$) for safrole (1) binding with free enzyme was determined to be 16.05 and $13.66{\mu}M$ for monophenolase and diphenolase, respectively.

Myristicae Semen Extract Protects Excitotoxicity in Cultured Neuronal Cells

  • Kim, Ji-Ye;Ban, Ju-Yeon;Bang, Kyong-Hwan;Seong, Nak-Sul;Song, Kyung-Sik;Bae, Ki-Whan;Seong, Yeon-Hee
    • 한국약용작물학회지
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    • 제12권5호
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    • pp.415-423
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    • 2004
  • Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on kainic acid (KA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to $5\;{\mu}g/ml$ inhibited KA $(500\;{\mu}M)-induced$ neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF $(0.5\;{mu}g/ml)$ inhibited glutamate release into medium induced by KA $(500\;{\mu}M)$, which was measured by HPLC. Pretreatment of MF $(0.5\;{mu}g/ml)$ inhibited KA $(500\;{\mu}M)-induced$ elevation of cytosolic calcium concentration $([Ca^{2+}]_c)$, which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents KA-induced neuronal cell damage in vitro.

$PPAR_{\gamma}$ Ligand-binding Activity of Fragrin A Isolated from Mace (the Aril of Myristica fragrans Houtt.)

  • Lee, Jae-Young;Kim, Ba-Reum;Oh, Hyun-In;Shen, Lingai;Kim, Naeung-Bae;Hwang, Jae-Kwan
    • Food Science and Biotechnology
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    • 제17권6호
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    • pp.1146-1150
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    • 2008
  • Peroxisome proliferator-activated receptor-gamma ($PPAR_{\gamma}$), a member of the nuclear receptor of ligand-activated transcription factors, plays a key role in lipid and glucose metabolism or adipocytes differentiation. A lignan compound was isolated from mace (the aril of Myristica fragrans Houtt.) as a $PPAR_{\gamma}$ ligand, which was identified as fragrin A or 2-(4-allyl-2,6-dimethoxyphenoxy)-1-(4-hydroxy-3-methoxyphenyl)-propane. To ascertain whether fragrin A has $PPAR_{\gamma}$ ligand-binding activity, it was performed that GAL-4/$PPAR_{\gamma}$ transactivation assay. $PPAR_{\gamma}$ ligand-binding activity of fragrin A increased 4.7, 6.6, and 7.3-fold at 3, 5, and $10{\mu}M$, respectively, when compared with a vehicle control. Fragrin A also enhanced adipocytes differentiation and increased the expression of $PPAR_{\gamma}$ target genes such as adipocytes fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and phosphoenol pyruvate carboxykinase (PEPCK). Furthermore, it significantly increased the expression level of glucose transporter 4 (GLUT4). These results indicate that fragrin A can be developed as a $PPAR_{\gamma}$ agonist for the improvement of insulin resistance associated with type 2 diabetes.

Protection of NMDA-Induced Neuronal Cell Damage by Methanol Extract of Myristica Fragrans Seeds in Cultured Rat Cerebellar Granule Cells

  • Ban, Ju-Yeon;Cho, Soon-Ock;Kim, Ji-Ye;Bang, Kyong-Hwan;Seong, Nak-Sul;Song, Kyung-Sik;Bae, Ki-Whan;Seong, Yeon-Hee
    • Natural Product Sciences
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    • 제10권6호
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    • pp.289-295
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    • 2004
  • Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on N-methyl-D-aspartate (NMDA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to $5\;{\mu}g/ml$, inhibited NMDA (1 mM)- induced neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF $(0.5\;{\mu}g/ml)$ inhibited glutamate release into medium induced by NMDA (1 mM), which was measured by HPLC. Pretreatrnent of MF $(0.5\;{\mu}g/ml)$ inhibited NMDA (1 mM)-induced elevation of cytosolic calcium concentration $([Ca^{2+}]_c)$, which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents NMDA-induced neuronal cell damage in vitro.

Antibacterial and Sporicidal Activity of Macelignan Isolated from Nutmeg (Myristica fragrans Houtt.) against Bacillus cereus

  • Rukayadi, Yaya;Lee, Kwan-Hyoung;Han, Sung-Hwa;Kim, Sung-Kyung;Hwang, Jae-Kwan
    • Food Science and Biotechnology
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    • 제18권5호
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    • pp.1301-1304
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    • 2009
  • Macelignan is a bioactive compound isolated from nutmeg (Myristica fragrans Houtt.) which has been traditionally used for the food and pharmaceutical purposes. In this study, the activities of macelignan against vegetative cells and spores of Bacillus cereus were evaluated in vitro. Our results showed that the vegetative cells of B. cereus were significantly inhibited in growth by macelignan with minimum inhibitory concentration (MIC) of $4{\mu}g/mL$. The vegetative cells of B. cereus were completely killed with minimum bactericidal concentration (MBC) of $8{\mu}g/mL$ of macelignan. Killing time of macelignan against vegetative cells of B. cereus was very fast; endpoint of macelignan was reached after 4 hr of incubation at $4{\times}MIC$. Macelignan inactivated more than 3-log (99.9%) of spores/mL of B. cereus at the concentration of $100{\mu}g/mL$. Macelignan was found to be effective against vegetative cells and spores of B. cereus. These results suggest that macelignan might be good to be developed as a food preservative.

국내산 약용식물 추출물에 대한 항균성 검색과 농도별 및 분획별 항균특성 (Antimicrobial Effect of Ethanol Extracts from Some Medicinal Herbs and Their Fractionates against Food-Born Bacteria)

  • 신동화;김문숙;한지숙
    • 한국식품과학회지
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    • 제29권4호
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    • pp.808-816
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    • 1997
  • 국내에서 생산되는 한약재 56종을 75% 에탄올로 추출하여 10종의 균주, Listeria monocytogenes ATCC 15313, 19111, 19112, 19113, 19114, Bacillus cereus YUFE 2004, Staphylococus aureus KFCC 11764, P. fluorescens 1645, 2344 and Leuconostoc mesenteroides KFCC 12031에 대하여 항균성을 비교한 결과 귤피, 연교, 우방자, 진교, 호황련, 희첨등은 몇 균주에 대하여 항균 효과를 보이는 반면 가자육, 금앵자, 소목 그리고 육두구는 각 균주에 대해 뚜렷한 증식억제효과를 보였다. 그중 소목 조추출물을 10 ppm 첨가수준에서 모든 시험 균주에 대해 뚜렷한 증식 저해 효과를 나타냈고 가자육은 L. monocytogenes ATCC 19114, B. cereus YUFE 2004, S. aureus KFCC 111764 그리고 P. fluorescens KCTC 1645에 대해 농도에 비례하여 상당한 항균효과를 보였다. 또한 뚜렷한 항균효과가 있는 추출물을 클로로포름, 에틸아세테이트, 부탄올로 분획하여 검색한 결과 대부분의 균주가 가자육, 금앵자, 소목 추출물은 에틸아세테이트 분획물에서 육두구는 클로로포름 분획물에서 더 높은 항균성을 보였다.

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장내 질환의 치료와 관련된 한약재의 장내 유해세균에 대한 항균 활성 (Antimicrobial Activity of Herbs with Treatments of Intestinal Diseases against intestinal Pathogens)

  • 이갑상;김성효;김선숙;박성수;전주연;신용서
    • 한국식품영양학회지
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    • 제11권1호
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    • pp.31-35
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    • 1998
  • In this study, we investigated the antimicrobial activity of herbs related with treatments of intestinal diseases against intestinal pathogens under anaerobic broth system. The water extract of Saussurea lappa Clarke and Myristica fragrans Houtt. showed no growth inhibition against tested pathogens(Eubacterium limonsum ATCC 10825, Escherichia coli ATCC 25922, Bacteroides fragilis KCTC 5013, Clostridium perfringens STCC 3627, Staphylococcus aureus KFCC 11764 및 Salmonella typhimurium ATCC 14028). All tested pathogens were not inhibited in broth containing 100$\mu\textrm{g}$/$m\ell$ of Areca catachu L. Water extract but its extract strongly inhibited the growth of Eubacterium limonsum STCC 10825, Bacteroides fragilis KCTC 5013, Clostridium perfringens ATCC 3627 and Salmonella typhimurium ATCC 14028 at 1,000 to 2,000$\mu\textrm{g}$/$m\ell$ of concentration. Escherichia coli ATCC 25922 and Staphylococcus aureus KFCC 11764 hardly grew in broth containing 2,000$\mu\textrm{g}$/$m\ell$ of Terminalia chebula Retz. water extract.

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