• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.622-629
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• 2018

Expansins are cell wall proteins that mediate cell wall loosening and promote specific tissue and organ morphogenesis in plants and in some microorganisms. Unlike plant expansins, the biological functions of fungal expansin-like proteins have rarely been discussed. In the present study, an expansin-like protein-encoding fvexpl1 gene, was identified from Flammulina velutipes by using local BLAST. It consisted of five exons with a total length of 822 bp. The deduced protein FVEXPL1 contained 274 amino acids with a predicted molecular mass and isoelectric point of 28,589 Da and pH 4.93, respectively. The first 19 amino acids from the N terminal are the signal peptide. Phylogenetic analysis and multiple protein alignment indicated FVEXPL1 was an expansin-like protein. The expression level of fvexpl1 gene in the stipe was significantly higher than that in the mycelia, primordia, and cap. However, the expression level of fvexpl1 gene was significantly higher in the fast elongation region of the stipe as compared with the slow elongation region. Expression analysis indicated that fvexpl1 gene might have an auxiliary role in the stipe morphogenesis of F. velutipes.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1998년도 천연항암자원개발에 관한 국제학술회의 논문집 및 1998년도 한국자원식물학회 정기학술발표회 논문요약
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• pp.48-50
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• 1998

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1212-1220
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• 2019

The study of metabolomics in natural products using the diverse analytical instruments including GC-MS, LC-MS, and NMR is useful for the exploration of physiological and biological effects and the investigation of drug discovery and health functional foods. Cordyceps militaris has been very attractive to natural medicine as a traditional Chinese medicine, due to its various bioactive properties including anti-cancer and anti-oxidant effects. In this study, we analyzed the metabolite profile in 50% ethanol extracts of C. militaris fruit bodies from three development periods (growth period, matured period, and aging period) using $^1H-NMR$, and identified 44 metabolites, which are classified as 16 amino acids, 10 organic acids, 5 carbohydrates, 3 nucleotide derivatives, and 10 other compounds. Among the three development periods of the C. militaris fruit body, the aging period showed significantly higher levels of metabolites including cordycepin, mannitol (cordycepic acid), and ${\beta}-glucan$. Interestingly, these bioactive metabolites are positively correlated with antitumor growth effect; the extract of the aging period showed significant inhibition of HepG2 hepatic cancer cell proliferation. These results showed that the aging period during the development of C. militaris fruit bodies was more highly enriched with bioactive metabolites that are associated with cancer cell growth inhibition.

• 한국균학회지
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• 제25권4호통권83호
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• pp.239-252
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• 1997

1990년 부터 1996년까지 16개소의 채집지에서 채집되어 분류동정된 동충하초는 12속 33종으로 이중 Cordyceps bifusispora, C. martialis, C. oxycephala, C. paludosa, C. pentatomi, C. rosea, C. ryogamiensis, Shimizuomyces paradoxa, Akanthomyces aculeatus, Polycephalomyces ramosus, Tilachlidiopsis nigra 등 14종은 국내 미기록종이다. 주로 다수 채집된 동충하초인 C. nutans, C. specocephala, Paecilomyces tenuipes 등은 전 채집시기에 걸쳐 고루 분포하였다. C. mizitayis C. kyushuensis, C. pruinosa 등과 같이 자실체의 발생이 환경조건으로서 대기내 습도에 강하게 영향을 받는 종들의 경우는 주로 장마철이 시작되면서 상대습도가 높아지는 7월 중순부터 8월 중순까지 분포하는 것으로 나타났다. 채집된 동충하초중 분리된 종은 C. bifusispora를 포함하여 Cordyceps속 9종, 불완전 균류의 Beauveria bassiana, Metarhizium anisopliae, Paecilomyces tenuipes, Verticillium lecanii등 총 13종이며, 분리된 Cordyceps속 동충하초를 이용하여 완전세대와 불완전세대의 관계를 구명한 결과 C. militaris와 C. kyushuensis는 Verticillium속으로, C. pruinosa는 Acremonium속으로, C. sphecocephala는 Hymenostilbe속으로, C. scarabaeicola는 Beauveria속으로 각각 밝혀졌다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.144.1-144
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• 1995

No Abstract, See Full Text

• 한국자원식물학회지
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• 제11권
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• pp.48-50
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• 1998

• Mycobiology
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• 제48권3호
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• pp.228-232
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• 2020

Cordyceps is a genus of ascomycete fungi and is well known as one of the important medical fungi in Chinese, Korea, and other Asian countries, because of its various beneficial effects on human health. The pharmacological activities of Cordyceps extract are mainly focused on anti-cancer, anti-metastatic, and immune modulating effects. In the present study, we investigated whether the antiplatelet effect of ethanol extract of cultured Cordyceps militaris (CMEE) with FeCl₃-induced arterial thrombosis model. We observed that CMEE exhibited a significant inhibitory effect against ADP and collagen-induced platelet aggregation. However, there were no significant differences in prothrombin time (PT) and activated partial thromboplastin time (aPTT). These results suggest that antithrombotic activity of CMEE is related to antiplatelet effect rather than anticoagulation effect, and CMEE may be a positive effect on improving blood circulation against vessel injury and occlusion.

• Mycobiology
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• 제48권6호
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• pp.512-517
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• 2020

Cordyceps militaris has been reported to the diverse pharmaceutical effects including cancer, inflammatory diseases, and bacteria or virus infection. However, the effect of C. militaris on exercise performance has not yet been elucidated. In this study, we investigated the beneficial effect of C. militaris on exercise performance. To evaluate exercise performance, we prepared C. militaris ethyl acetate extract (CMEE) and conducted grip strength tests every week after administration. Additionally, blood samples were collected at the end of the experiment for biochemical analysis. The administration of CMEE slightly increased grip strength, and this result was similar to the red ginseng treated group. According to the result of biochemical analysis, CMEE had an effect on the biomarkers related to ATP generation pathway but had little influence on the muscle fatigue related biomarkers. Therefore, C. militaris has the possibility of improving exercise performance, which could be associated with the increase in ATP production rather than the decrease in muscle fatigue during exercise.

• 한국임상수의학회지
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• 제21권1호
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• pp.35-44
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• 2004

This study was performed to examine the mycological features of canine skin. A total of 50 dogs with skin lesions were examined for dermatology from October, 2000 to April, 2001. The isolation rates of dermatophytes, yeast, filamentous fungi and superficial fungi were 36.4%, 13.5%, 35.3% and 13.6%. The dermatophytes isolated in dogs were Microsporum canins and Trichophyton mentagrophytes were 75% and 25%. The yeast and superficial fungi isolated in dogs were Candida albicans, Rhodntorula minnata, Candida ceferrii and Malassezia spp. were 16.7%. 16.7%, 16.7% and 50%. The filamentous fungi by Aspergillus funigatus, Aspergillus niger, Penicillum spp., Alternaria spp. were 12.5%, 12.5%, 50%, and 25%. In determine if polymerase chain reaction (PCR) could be applied for diagnosis of dermatophytes, yeast and filamentous fungi, control and clinical samples were tested. The size of specific PCR product in agarose gel was 340 bp for dermatophytes and 210 bp for yeast and filamentous fungi, respectively.

• 한국균학회지
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• 제51권4호
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• pp.373-381
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• 2023

This study aimed to isolate and characterize wild yeast strains from the vineyard soil in Korea. Twenty yeast strains were isolated from vineyard soil in Gimpo-si, Gyeonggi-do, Korea, where Vitis labrusca cv. Campbell Early is grown. Eighteen strains were previously recorded in Korea. The remaining two, Cyberlindnera mrakii VG-21-10C and Starmerella bacillaris GR9 were not previously recorded in Korea. The mycological characteristics of VG-21-10C and GR9 were investigated. Both were oval-shaped, convex, and smooth. However, differences were evident in colony color and carbon assimilation activities. Strain VG-21-10C is white-colored and assimilates glucose, glycerol, D-xylose, D-cellobiose, D-saccharose, and D-raffinose as carbon sources. Strain GR9 is cream-colored and assimilates glucose, D-saccharose, and D-raffinose as carbon sources.