• Title/Summary/Keyword: Mycelium growth

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Effect of Polyacrylic Acid Addition on Wall Growth in Submerged Cultivation of Ganoderma lucidum (영지 액체 배양의 Wall Growth에 미치는 Polyacrylic Acid의 첨가 효과)

  • Lee, Shin-Young;Lee, Hak-Su
    • Journal of Industrial Technology
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    • v.21 no.A
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    • pp.337-341
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    • 2001
  • This study was carried out to screen the effective polymeric additives preventing wall growth during mycelial submerged cultivation of Ganoderma. lucidum. Effects of additives on mycelial growth and exo-polysaccharide (EPS) production in flask culture and jar fermenter system under 3 different pH processes were investigated, and changes of mycelial morphology were also examined. From flask culture of G. lucidum with additives of different concentrations, 0.1%(w/v) polyacrylic acid was effective for EPS production. As the polyacrylic acid of 0.1%(w/v) was added in medium, wall growth of G. lucidum mycelium grown in jar fermenter system could be protected. The addition of 0.1%(w/v) polyacrylic acid to medium was also improved the mycelial growth and EPS production in the later of submerged culture G. lucidum and no changes of mycelial morphology were observed.

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Studies on the Incubation Condition according to Carbon Source, Nitrogen Source and Isolation of Pathogenic Aspergilli from Lung of Domestic Fowls (닭의 폐(肺)에서 병원성(病原性) Aspergilli 의 분리(分離)와 탄소원(炭素源)및 질소원(窒素源)에 따른 배양조건(培養條件)에 관(關)하여)

  • Lee, Bae-Ham;Lee, Kyoung-Bae
    • The Korean Journal of Mycology
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    • v.1 no.2
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    • pp.25-30
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    • 1973
  • The Aspergilli can be isolated from many of damaged domestic fowl's lung which were collected at fowl slaughter house from July to August 1973, during two months at Dapsimri, Dongdaemoon-ku, Seoul city. The survey of incubation condition for carbon source and nitrogen source were summerized as follows: 1. One of the Aspergillius fumigatus group were isolated from damaged domestic fowl's lung. 2. In the preparation of isolation media, adding the 4 gs of silkworm pupa and Meju to Czapek's agar medium is more preferable than Austick's media (2% malt extract agar) 3. In survey of incubation condition, the growth rate and sporulation shown that higher differences according to varieties and amounts of C-source. And in the case of 20% C-source concentration shown that low growth rate than lower concentration of C-source. 4. In N-source, the growth rate indicated that some difference according to varieties of N-source, difference of growth rate demonstrated almost not shown at various amounts differences of mycelium and sporulation was only detected reversely.

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Studies on the Possidility of Oak Mushroom(Shiitake) Cultivation on Ban Oak (Quercus incana) of India (인도산(印度産) 참나무(Quercus incana)를 이용한 표고재배(栽培)의 가능성(可能性)에 관한 연구(硏究))

  • Lee, Eung-Rae
    • The Korean Journal of Mycology
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    • v.6 no.2
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    • pp.31-36
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    • 1978
  • The study has been carried out to invetigate the possibility of oak mushroom (shiitake) cultivation on Ban Oak (Quercus incana) growing naturally in Simlaregion, India. The survival and growth of oak mushroom mycelium and fruit body formation on Q. incana as well as the composition of the log were compared with that of Bristle-tooth oak (Q. serrata) which are being used for mushroom cultivation in Korea. The results are summarized as follows. 1. The content of alcohol-benzen extract, NaOH extract, NaOH extract and ash were higher in Q. incana than in Q. Serrata. While cellulose and pentosan were less in the formers. 2. The mycelial growth of oak mushroom were more rapid on sawdust medium of Q. Serrata than on Q. incana. However the mycelial growth on the later were more compact. 3. The mycelial growth of oak mushroom were more rapid on the logs of Q. serrata than Q. incana. The mycelium survived well on both two species, and no difference in the survival rate of mycelium were observed. 4. The first fruit bodies on logs of Q. Serrata and Q. incana were appeared 16 months inoculation of spawn. 5. In view of the above results. it seems that the cultivation of oak mushroom (shiitake) on Ban oak (Q. incana), growing in India, is possible.

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Effect of Various Plant Extracts on the Mycelial Growth and Fibrinolytic Activity of Armillaria mellea (식물유래 물질이 뽕나무버섯(Armillaria mellea) 균사체 생장 및 혈전분해 활성에 미치는 영향)

  • Choi, Han-Seok;Kim, Myung-Kon;Park, Hyo-Suk;Kim, Jae-Sung;Kim, Sung-Jun
    • The Korean Journal of Mycology
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    • v.33 no.1
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    • pp.11-17
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    • 2005
  • To increase the fibrinolytic activity and production of mycelium, extracts of 7 plant species were supplemented to the growth media of Armillaria mellea, and mycelial growth and enzymatic activity in the mycelium extracts of A. mellea were estimated. The mycelial production of A. mellea was slightly increased by adding ASH-R, UDVN or RGR extract, whereas KG extract significantly affected the growth. Supplement of ASH-S, UDVN and RGR extracts increased proteolytic activity from 36.8 to 46.1% Fibrinolytic activity was increased to $50{\sim}65%$ by supplement with RVS, ASH-S and RGR extracts, respectively. Enzyme extracts of the fungus grown with RGR extract supplement degraded all chains of fibrinogen within 2 hours, whereas control was required 3 hours. Degradation of fibrin fragments by the enzyme extracts was also observed through microscopy.

Enhancement of Immune Activities of Ganoderma lucidum Mycelium Cultured with Garlic Enriched Medium (마늘 첨가 복합배지에서 배양된 영지 균사체의 면역 증진 효과)

  • Mun, Hyoung-Chul;Lee, Hyun-Soo;Park, Jin-Hong;Kim, Dae-Ho;Lee, Shin-Young;Seong, Nak-Sul;Bang, Jin-Ki;Jung, Hae-Gon;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.1
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    • pp.24-30
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    • 2004
  • The immune activities of Ganoderma lucidum Mycelium added garlic extracts (GAM), Ganoderma lucidum Mycelium (GM), garlic extracts (GS) and standard $({\beta}-glucan)$ were compared. GAM enhanced the growth of human immune T cell up to $1.25{\sim}1.46$ times, compared to control group. GAM showed relatively lower cytotoxicity in using normal human lung cell, while GAM showed the most potent inhibitory effect on the human lung carcinoma, compared to GM and GS. The selectivity of GAM was also higher than that of GM and GS. GAM increased the secretion of cytokines, IL-6 and TNF- from human B cell as well as the growth of human immune cells. It can imply that GAM has higher immune activity than GM or GS.

Cultural Characteristics for the Enhanced Mycelial Growth of Ramaria botrytis

  • Lee, Tae-Hee;Han, Yeong-Hwan
    • Mycobiology
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    • v.33 no.1
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    • pp.12-14
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    • 2005
  • The culture conditions for the enhanced mycelial growth of Ramaria botrytis was investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and 5.0, respectively. It was shown that starch was best of several carbon sources in Czapek-Dox medium as a minimal medium for the enhanced mycelial growth. Organic nitrogen sources were better than inorganic ones for mycelial growth. The appropriate vitamin and mineral salt were biotin and FeCl3, respectively. When this strain was cultured with $FeCl_3$ for 30 days, 19.23 g/l of dry mycelium of R. botrytis was obtained.

Effect of "Green-one" organic nutrient on mycelial growth and fruiting of Pleurotus ostreatus (유기농 자재 『그린원』이 느타리버섯(Pleurotus ostreatus)의 균사생장 및 자실체 생육에 미치는 영향)

  • Chang, H.Y.;Kim, S.J.;Kim, J.K.;Kim, J.H.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.12 no.1
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    • pp.21-27
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    • 2010
  • The objective of this study was to determine the effects of "Green-one" organic nutrient on mycelial growth and fruiting of P. ostreatus. The dilution concentrations of "Green-one" was treated as follows. There was control, 100, 200, 400 concentrations. That treatments were treated with step of each mycelial growth step. The best of growth steps was mycelial scratching step. At that time, DPI(Day required for primordial formation after inoculation) was shortened for 1 day. Valid germination stipe are 15 pieces, 3 pieces more than control. Stipe length and stipe diameter was long each 4mm, 3mm more than control. Pileus size is shortened than control. Yields per one bottle(g/850cc) was 146g increased 6.5% than control 137g/850cc.

Characteristics of Red Mold Isolated from Traditional Meju (전통메주에 증식하는 붉은 곰팡이의 특성)

  • 이상원;박석규;김홍출
    • Food Science and Preservation
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    • v.8 no.2
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    • pp.199-205
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    • 2001
  • Red mold was isolated from meju prepared by traditional mtehod and characterized. The isolated red mold grew well on potato dextrose agar medium, In microscopic observation, it had a septum in mycelium and ellipsoidal spore. Optimal temperature and pH for growth were 30$\^{C}$ and 6.0, respectively. Enzyme activities such as protease, a-amylase and glucoamulase in ted mold were lower than those in Aspergillus oryzae. A competitive growth between red mold and Asp. oryzae was greatly affecten by cultivation temperature. The growth of isolated red mold on meju was predominant at below 30$\^{C}$ as compared with Asp. oryzae.

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Influence of Moisture on Mold Growth in Building Materials (건축자재 내의 수분이 곰팡이 성장에 미치는 영향)

  • Seo, Janghoo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.24 no.12
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    • pp.852-857
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    • 2012
  • Recently, the indoor air pollution by microbes such as fungi and mites have become a concern as important research topic on indoor air quality. Fungal growth is significantly affected by humidity. In this study, we examined the influence of relative humidity on the surface of building materials and the water content of building materials on the fungal growth rate by measuring the mycelium length of fungi in the fungal detector placed on the surface of building materials. As a result, even if the relative humidity on the surface of building materials is identical, the more water content of building materials is, the more fungi grow faster. It was suggested that fungal growth rate depends on not only the relative humidity on the surface of building materials but also the water content of building materials.

Temperature & Humidity Data Analysis for automatically controlled environment needed to develop mushroom crop (버섯농작물 육성에 필요한 온·습도 자동조절을 위한 재배자료 분석)

  • Lee, Hyun-chang;Jin, Chan-Yong;Shin, Seong-yoon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.10a
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    • pp.387-388
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    • 2016
  • Among agricultural products, environmental temperature and humidity is an important factor that affects the growth of mushrooms. Mushrooms grow well in a certain temperature range, but if mushrooms deviate from the appropriate temperature range, mushrooms growth and development quality will become worse, and even stop growing. In this paper, the status of the mushroom bacteria, the growth of the mycelium, the occurrence of the fruiting bodies and the growth of the fungus were investigated, o as to provide reference for cultivation management technology of mushroom precise cultivation.

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