• Mycobiology
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• 제47권1호
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• pp.97-104
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• 2019

Mushroom cultivation has gained increased attention in recent years. Currently, only four types of spawn, including sawdust spawn, grain spawn, liquid spawn, and stick spawn, are commonly available for mushroom cultivation. This limited spawn diversity has led to difficulty in selecting suitable inoculum materials in some cultivation. In this study, three small blocks of lignocellulosic agro-wastes and one block of a synthetic matrix were prepared as support for growing Pleurotus ostreatus in liquid medium. Mycelium-adsorbed blocks were then evaluated for their potential as block spawn for fructification. Our results indicated that the edible fungus was adsorbed and abundantly grew internally and externally on loofah sponge and synthetic polyurethane foam (PUF) supports and also has the ability to attach and grow on the surface of sugarcane bagasse and corncob supports. The mycelia of P. ostreatus adhered on corncob exhibited the highest metabolic activity, while those on the PUF showed the least activity. Mycelial extension rates of block spawns made of agro-waste materials were comparable to that of sawdust spawn, but the block spawn of PUF showed a significantly lower rate. No significant differences in cropping time and yield were observed among cultivations between experimental block spawns and sawdust spawns. Moreover, the corncob block spawn maintained its fruiting potential during an examined period of 6-month storage. The developed block spawn could be practically applied in mushroom cultivation.

• 한국균학회지
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• 제38권2호
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• pp.125-129
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• 2010

잣버섯에 적합한 액체종균용 주배지 재료는 대두분, 배양 기간은 12일, 통기량은 0.9 vvm이 적합하였다. 또한, 잣버섯 재배용 톱밥종균용 배지는 미송과 옥분을 부피비로 95 : 5로 혼합하는 것이 적합하였다. 액체종균 및 톱밥종균을 사용하였을 경우 잣버섯 생산성을 비교한 결과 액체종균으로 재배한 경우 재배일수 43일로 톱밥종균으로 재배할 때보다 재배기간 보다 2일 단축되었고, 유효경수 11.3개, 수량 111.9 g로 우수하였다.

• Journal of the Korean Wood Science and Technology
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• 제26권1호
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• pp.19-28
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• 1998

For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows: 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was $25^{\circ}C$, and $22.5\sim27.5^{\circ}C$ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106mg dry mycelia per 40ml media after 17 days. 2. In 1l-spawn bottle, growth of mycelium by inoculation of 20ml-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10ml-, 20ml- and 30ml-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30ml-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20ml-liquid spawn and 10ml-liquid spawn, respectively. Thus, 30ml-liquid spawn inoculation was highest in yield.

• 한국버섯학회지
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• 제12권4호
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• pp.237-243
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• 2014

Spawn is the vegetative growth of the mycelium and serves as the inoculum or seed for cultivating mushrooms. Various cereal grains, such as wheat, millet, barley, sorghum, brown rice, rye, and oat were assessed to compare their characteristics and suitability for spawn production of button mushroom (Agaricus bisporus). The mycelial growth rates, density, and the number of completely colonized grains were measured from the twentieth day of inoculation. Wheat grains showed fastest mycelial growth with 8.4 cm followed by rye, oat, barley with 8.2, 7.5 and 7.3 cm, respectively. In the mycelial density, foxtail millet, barley, and sorghum were best compared with that of wheat grains. Especially, the number of grains which were completely colonized by mycelia were greatest in foxtail millet with 5,123 grains followed by proso millet, and wheat with 3,052 and 914, respectively. Based on the results obtained, barley, foxtail millet, and sorghum grains would be appropriate substituting for wheat grain in spawn production of button mushroom.

• 한국균학회지
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• 제49권4호
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• pp.525-530
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• 2021

The cultivation in sawdust media, mushroom productivity, and fruiting body characteristics of Lentinula edodes strains NIFoS 2778 and NIFoS 3363 were compared according to the inoculation conditions. The cultivation period was 5% shorter when liquid spawn was used. Fruiting bodies were induced after 113 days of incubation on media inoculated with liquid spawn, and the cultivation period was 119 days on media inoculated with solid spawn. Mushroom productivity of NIFoS 2778 was the highest (661.4 g) when 36 mL of liquid spawn was used. For NIFoS 3363, mushroom production was higher under liquid inoculation conditions when the same amount of liquid and solid spawns were used. The mushroom characteristics of the two strains were not significantly different, except for gill width and stipe diameter.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.1031-1040
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• 1996

Spawn bottle cutting and spawn cake shredding machine has been developed to save the farm labor and operating cost for mushroom growing farmers. The prototype can cut the bottom and side of the bottle while shredding the spawn cake by one farmer. The cutting plastic shell is done by two couples of high speed disc saws that can cut cross section and lengthwise two side of the bottle, while spawn cake shredding is done by spike teethed rotating drum and wiremesh concave. The optimum speed of cutting disc saw was observed 1.700rpm both the cross cutting saw and lengthwise cutting saw in consideration of the cutting accuracy. And the location for the instalation of cross cutting disc saw was considered as around 4 mm above the table bottom , while the optimum clearance between two edges of lengthwise cutting disc saw was showed 86mm. For the sawdust spawn cake shredder , proper size of spawn sawdust granule was observed in the 15 x 15mm size withmesh concave with the shredding rum speed of 500rpm. The prototype can be reduce 73 per cent of working hours with the working cost reduction of 49 per cent compare with those of conventional operation.

• 한국버섯학회지
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• 제11권3호
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• pp.145-148
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• 2013

목이버섯의 톱밥종균을 제조하는데 참나무톱밥은 영양원으로 미강을 20%첨가하고, 포플러톱밥은 밀기울을 10%첨가하였을 때 목이버섯의 균사와 밀도가 양호하였다. 이때 봉지재배에 적합한 종균 접종양은 참나무톱밥종균은 15 g, 포플러톱밥종균은 25 g를 접종할 때 오염발생률이 줄고 배양완성률이 높아 적합한 접종양 이었다.

• Mycobiology
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• 제33권1호
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• pp.19-22
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• 2005

We investigated the effect of nitrogen fixing Azotobacter bioinoculant on the mycelial growth and the rate of substrate utilization by Pleurotus eous. The synergistic or antagonistic role of the microorganism during dual culturing with the mushroom or the competitor molds Trichoderma viride, and Trichoderma reesi was studied. Azotobacter was inhibitory to the molds, which are competitive to the mushroom in the seed spawn substrate, but was synergistic towards the mushroom. The growth, substrate utilization potential as total nitrogen content and cellulase enzyme activities of the mushroom in the seed spawn substrate were also enhanced in the presence of the bioinoculant at lower inoculum concentrations, upto 5 ml broth culture per spawn bottle.

• 한국버섯학회지
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• 제21권4호
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• pp.200-208
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• 2023

본 연구는 한중 FTA 이후 신선 표고버섯의 수입량이 감소한 원인을 살펴보기 위해 신선 표고버섯 수입량에 대하여 접종배지 수입량과 환율을 이용하여 계량경제적 방법론으로 분석하였다. 분석자료는 2009년 1월부터 2022년 12월까지 월별 시계열 자료이다. 분석 결과, 신선 표고버섯 수입량을 감소시키는 주요 원인 중 하나는 접종배지 수입량으로 나타났다. 한중 FTA에서 접종배지는 관세를 감축하는 양허대상이다. 따라서 시간이 지남에 따라 관세감축이 이루어지면 수입가격 또한 하락하면서 가격 경쟁력을 가지게 된다. 또한 한중 FTA 원산지 규정에 따라 수입 접종배지에서 생산된 표고버섯은 국내산으로 인정받는다. 따라서 낮은 수입가격을 바탕으로 가격 경쟁력을 가진 수입 접종배지를 이용한 표고버섯의 생산이 중국산 신선 표고버섯을 수입하는 것보다는 매력적일 것이다. 이러한 결과는 VAR 모형을 분석한 결과에서도 같았다. 다만, 신선 표고버섯 수입량의 동태적 변화에서는 접종배지 수입량 변화의 충격이 표고버섯 수입량을 증가시킬 수 있다는 사실을 확인할 수 있었다. 그 원인은 수입산 접종배지가 오염되어 폐기처분에 따른 생산량 감소 또는 국산 접종배지의 신선 표고버섯 생산량이 감소한 것이라고 추론할 수 있다. 그러나 명확한 원인을 판단하기 위해서는 접종배지의 원산지에 따른 신선 표고버섯 생산량을 관측하고 통계 자료를 구축하는 것이 필요하다.

• 한국버섯학회지
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• 제8권1호
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• pp.11-15
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• 2010

느타리버섯 재배시 현재 톱밥병종균을 대체할 수 있는 저비용 우량봉지제조기술을 개발하기위해 시험을 수행한 결과는 다음과 같다. 1. 재료의 산도 (pH)는 포플러톱밥이 pH 6.7, 미강은 pH 6.4에 비하여 비트펄프는 pH 4.9로 다른 배지재료에 비하여 산성을 나타내었다. T-C는 포플러톱밥 57.5%, 면실피 54.5%, 미강 52.4%로 나타났고, T-N는 포플러톱밥이 0.1%로 면실피 1.77%, 미강 0.94%로 나타났다. 2. 균사생장정도는 신농73호 2.5kg 규격 종균의 필터가 구비된 종균의 경우 15.7cm, 마개형 10.5cm, 필터가 없는 종균은 7.5cm로 필터구비종균이 생장이 우수하였고, 균사밀도의 경우에도 필터형이 밀도가 높았다. $CO_2$ 농도는 신농73호 2.5kg 규격 종균의 필터가 구비된 종균의 경우 1,400ppm, 마개형 2,200ppm, 필터가 없는 종균은 3,200ppm 로 필터구비종균이 농도가 낮음을 알 수 있다. 3. 배양소요일수를 측정한 결과, 신농 73호에서 2.5kg 필터종균의 경우 18일, 마개필터 18일, 필터가 없는 처리는 19일로 큰 차이는 없었다. 생산량의 경우 신농73호에서 2.5kg 필터종균의 경우 118g, 마개필터 112g, 필터가 없는 처리는 100g으로 필터처리 종균이 생산력 측면에서 우수하였다.