• 제목/요약/키워드: Muscle properties

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Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development

  • Kim, Nam-Kuk;Lim, Jong-Hyun;Song, Min-Jin;Kim, Oun-Hyun;Park, Beom-Young;Kim, Myung-Jick;Hwang, In-Ho;Lee, Chang-Soo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.10
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    • pp.1612-1617
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    • 2007
  • In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development. Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Four proteins were identified as myosin light chain 1 slow-twitch (MLC1sa) isoform, troponin T, triosephosphate isomerase (TIP) and DJ-1 protein. The remaining protein was not identified. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and then their levels were decreased markedly during growth stages. On the other hand, the expression of TIP and DJ-1 protein, which are well known as catalysis enzyme and antioxidant-related protein, respectively, were linearly increased during growth stages. Thus, the stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle.

Postmortem changes in physiochemical and sensory properties of red snow crab (Chionoecetes japonicus) leg muscle during freeze storage

  • Jun, Joon-Young;Jung, Min-Jeong;Kim, Dong-Soo;Jeong, In-Hak;Kim, Byoung-Mok
    • Fisheries and Aquatic Sciences
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    • v.20 no.7
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    • pp.13.1-13.7
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    • 2017
  • In order to evaluate the maximal storable period of the raw crab for a non-thermal muscle separation, the quality changes of the leg meat of red snow crab (Chionoecetes japonicus) during freeze storage were investigated. Fresh red snow crabs were stored at $-20^{\circ}C$ for 7 weeks, and the leg muscle was separated by a no heating separation (NHS) method every week. During the storage, considerable loss of the leg muscle did not occur and microbiological risk was very low. In contrast, discoloration appeared at 2-week storage on around carapace and the leg muscle turned yellow at storage 3-week. In physiochemical parameters, protein and free amino acids gradually decreased with storage time, expected that proteolytic enzymes still activated at $-20^{\circ}C$. At 4-week storage, the sensory acceptance dropped down below point 4 as low as inedible and notable inflection points in pH and acidity were observed. The volatile base nitrogen was low, though a little increase was recorded. These results suggested that the maximal storable period at $-20^{\circ}C$ of the raw material was within 2 weeks and it was depended on external factor such as the discoloration. The present study might be referred as basic data for approaches to solve quality loss occurred in non-thermal muscle separation.

Physicochemical attributes, oxidative stability, and microbial profile of boneless sirloin and bone-in T-bone steaks from Hanwoo steer with reference to dry-aging

  • Ali, Mahabbat;Nam, Ki-Chang
    • Journal of Animal Science and Technology
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    • v.63 no.5
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    • pp.1169-1181
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    • 2021
  • We investigated the comparative physicochemical attributes, oxidative stability, and microbial characteristics of 28 days dry-aged meat in between boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) muscles from Korean Native Hanwoo Steer (KNHS). Results reveal that regardless of the muscles, dry-aging increased protein content and water-holding capacity (WHC) (p < 0.05). Meat from infraspinatus-aged muscle led to darker meat with higher pH values than un-aged meat (p < 0.05). However, fat content, CIE a*, and CIE b* remained unchanged in both muscles at aging. At aged meat, thiobarbituric acid reactive substances (TBARS) values from bone-in infraspinatus muscle was 2.5-fold higher than boneless gluteus medius muscle (p < 0.05). Dry-aging led to an increase in the contents of total unsaturated fatty acids (UFAs), monounsaturated fatty acids (MUFAs), and UFA/saturated fatty acids (SFA) in both muscles (p < 0.05). Furthermore, gluteus medius aged muscle concentrated with olic acid (C18:1) compared to infraspinatus aged muscle. Irrespective of the muscles, dry-aging enhanced the total free amino acids (FAAs) as well as tasty, and bitter amino acid contents whereas decreased the tasty/bitter amino acids (p < 0.05). Aromatic amino acid, tryptophan that converted to serotonin was 2-fold higher in boneless gluteus medius muscle than bone-in infraspinatus muscle at pre and post aging processes (p < 0.05). Aged Infraspinatus muscle increased total bacteria (p < 0.05) while no salmonella spp. was detected in both muscles. Taken together, our study confirms that 28 days dry-aging profiling the quality characteristics of boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) distinctly while gluteus medius aged steak performs better owing to oxidative stability and functional compounds than infraspinatus aged steak.

Analysis of Upper Limb Muscles Properties In Elite Swimmers Before and After Training (엘리트 수영선수들의 수중 훈련 전후의 상지 근육 특성 변화 분석)

  • Raphael Kihong Koo;Hyunwoo Kang;Seong Won Park;Taewhan Kim
    • Korean Journal of Applied Biomechanics
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    • v.33 no.3
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    • pp.101-109
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    • 2023
  • Objective: The aim of this study is to verify the differences in muscle characteristics of elite level swimmers before and after a 2-hour practice session. Method: The study was conducted on 15 elite swimmers. Preliminary measurements for each muscle (Anterior Deltoid, Triceps Brachii, Biceps Brachii, Flexor Carpi Ulnaris) were taken using the MyotonPRO device before training. After approximately 2 hours of training, the same muscle areas were measured again. The collected data was analyzed through descriptive statistics and two-way 2×2 RG·RM ANOVA, and all statistical significance levels were set at α=.05. Results: After analyzing the characteristics of the Flexor Carpi Ulnaris (FCU) before and after training in both proficiency level swimmers (excellent, non-excellent), it was found that the interaction effect of group X repetition in muscle tension (F), muscle stiffness (S), and body recovery time (R) was statistically significant. Secondly, in the analysis of the Biceps Brachii (BB), the main effect of repetition in muscle tension (F), muscle stiffness (S), and body recovery time (R) was statistically significant. Furthermore, the interaction effect of group X repetition in muscle stiffness (S) and body recovery time (R) was statistically significant. Conclusion: The efficient use of FCU and BB suggests that it is an important factor distinguishing the performance of excellent and non-excellent swimmers in swimming. Therefore, if we develop and apply measures to efficiently utilize FCU and BB during training, it can help improve the performance of the athletes.

Effects of Freezing-Thawing Conditions on Physicochemicnl and Histological Properties of Plaice, Paralichthys olivaceus Muscle (동결-해동 조건이 넙치육의 물리$\cdot$화학적 및 조직학적 변화에 미치는 영향)

  • CHO Young-Je;CHO Min-Sung;LEE Nam-Gul;CHOI Young-Jun;KIM Tae-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.4
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    • pp.463-470
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    • 1998
  • To improve muscle quality and prolong freshness of sashimi, the effects of freezing-thawing condition on physicochemical and rheological properties of plaice muscle were investigated. Muscle tested were frozen with quick freezing (liquid nitrogen gas) or slow freezing ($-15^{\circ}C$ air). Transition time of zone of ice crystal formation was within 10 minute for quick freezing and 110 minute for slow freezing. Time required for thawing to $0^{\circ}C$ in muscle temperature by various thawing methods was shortest with $25^{\circ}C$ tap water, followed by $15^{\circ}C$ tap water, $10^{\circ}C$ tap water, $25^{\circ}C$ air, $5^{\circ}C$ tap water and $0^{\circ}C$ cold water. Breaking strength of muscle was higher in quickly frozen sample than in slowly frozen sample. According to sashimi term, changes in breaking strength of muscle did not show any difference in quickly frozen sample, while showed significant difference in slowly frozen sample. The remaining content of ATP was not effected by freezing speed, and ATP content was apt to higher in quickly thawed sample than in slowly thawed sample. IMP was the majority of ATP and it's related compounds of sample after freezing and thawing. Collagen matrix was weakened markedly in slowly frozen sample than in quickly frozen sample.

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Muscle Fiber, Connective Tissue and Meat Quality Characteristics of Pork from Low Birth Weight Pigs as Affected by Diet-Induced Increased Fat Absorption and Preferential Muscle Marbling

  • Bimol C. Roy;Patience Coleman;Meghan Markowsky;Kun Wang;Yongbo She;Caroline Richard;Spencer D. Proctor;Heather L. Bruce
    • Food Science of Animal Resources
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    • v.44 no.1
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    • pp.51-73
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    • 2024
  • This study investigated how birth weight differences in piglets affected carcass and muscle fiber properties as well as meat quality at slaughter. Within litters, piglets were grouped according to their birth weight as either normal (NBW; 1.62-1.73 kg) or low (LBW; 1.18-1.29 kg). At 5 weeks of age, NBW piglets were randomly transitioned to control (C) or isocaloric high fat diets derived from non-dairy (HF), while LBW piglets were randomly transitioned to high fat diets derived from non-dairy (HF) or dairy sources (HFHD). Piglets were reared in individual pens under standardized housing and feeding conditions. Live weight was recorded weekly, and pigs were slaughtered at 12 weeks of age. Hot carcass weights, dressing percentages, lean meat yield, and primal cut proportions were determined. The m. longissimus thoracis was collected from the right side of the carcass for measurement of physical and chemical properties of meat and muscle fiber characteristics. Results indicated that LBW pigs compensated for their live weight compared to NBW pigs at 6 weeks of age. The mean muscle fiber diameter of LBW-HFHD group is significantly higher than NBW-C and NBW-HF group, and the type I muscle fiber diameter is significantly higher than NBW-C group. Dairy fat inclusion in LBW pig diet reduced carcass back fat thickness. This increased the calculated lean meat yield to be comparable to that of NBW pigs fed a commercial diet. Incorporating dairy-sourced high-fat into LBW pigs' diets appears to be an effective strategy for producing carcasses equivalent to NBW pigs.

Alterations in the Neuro-Mechanical Properties of Human Ankle Dorsiflexor after Maximum Eccentric Exercise (최대 신장성 수축운동에 따른 인체 하지 족배굴곡근의 신경-기계학적 특성 변화)

  • Lee, Hae-Dong;Kim, Seung-Jae;Kawakami, Yasuo
    • Korean Journal of Applied Biomechanics
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    • v.18 no.4
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    • pp.21-30
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    • 2008
  • The purpose of this study was to investigate changes in mechanical properties of human tibialis anterior following eccentric exercise. Healthy subjects (n=12) performed 120 maximum eccentric contraction of ankle dorsiflexor. Before and 1- and 24- hour after the eccentric exercise, ankle dorsiflexion moment-angle relationships were obtained. Along with significant decrease in maximum isometric muscle strength, the shift of the optimum ankle joint angle toward the longer muscle length direction was observed, independent of the ranges of motion of the eccentric exercise. The results of this study demonstrated that eccentric exercise-induced micro muscle damage(Morgan & Allen, 1999) does rut seem to be a sole mechanism of eccentric contraction-induced muscle damage, suggesting further investigation for the better understandings of this phenomenon.

Meat Quality, Textural and Sensory Properties of Farm-Grown Pheasant Meat and Processed Products (농장 사육 꿩고기의 육질 및 가공제품의 물성과 관능특성)

  • 오홍록;유익종;최성희
    • Food Science of Animal Resources
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    • v.24 no.1
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    • pp.73-79
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    • 2004
  • Functional properties of farm-grown pheasant meat with different sex, age and cutting portion were investigated, and the textural and sensory characteristics of processed products were also evaluated. Chemical composition of pheasant meat was characterized to be high in protein and low in fat, and breast muscle showed more protein and less moisture than thigh muscle. Moisture/protein ratio of the pheasant meat was relatively low in a range of 2.82∼3.40, indicating the pheasant meat would be a good source of processed meat, and it had high water holding capacity and myofibrillar protein extractability with some variations depending on age and portion cut(p<0.05). Thigh muscle showed higher value of L* and b* and lower value of a* than breast muscle. However, no difference was observed in color of meat with different age and sex. The meat from the 6 months and the breast cut had lower shear force than those of respective 17 months and the thigh regardless of sex. The pressed ham and sausage manufactured with the pheasant meat had better score than the commercial products manufactured with pork or chicken in sensory and textural parameters.