• Journal of Korean Physical Therapy Science
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• v.2 no.3
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• pp.587-598
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• 1995

The purpose of this study was to test the microcurrent electrical neuromuscular stimulation on muscle soreness, serum creatine kinase levels and force deficits evident following a high-intensity eccentric exercise bout. 10 volunteer male subjects were randomly assigned to a treatment group or to a control group. Exercise consisted of high-intensity eccentric contractions of the elbow flexors. Resistance was reduced as subjects fatigued, until they reached exhaustion. Muscle soreness rating was determined using a visual analog scale. Serum creatine kinase levels were analyzed using a blood sample. Force deficits were determined by measures of maximal voluntary isometric contraction at $90^{\circ}$ of elbow flexion on a Orthotron II dynamometer. Muscle soreness rating, serum creatine kinase levels and maximal voluntary isometric contraction were determined at the before exercise and again at 24 and 48 hours postexericse. Treatments were applied immediately following exercise. The control group subjects rested following their exercise bout. Statistical analysis showed significant increases in muscle soreness rating and significant decreases in maximal voluntary isometric contraction when the before exercise was compared with 24 and 48 hour measures(p<0.01). No significant effects were observed between groups in muscle soreness rating and maximal voluntary isometric contraction(p>0.05). Highly significants differences in serum creatine kinase levels were found using on Analysis of variance(ANOVA) repeated measures between groups for each time cycles(p<0.001). This modality may have benefits when used early stage in the muscle damage.

• Korean Journal of Exercise Nutrition
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• v.24 no.1
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• pp.29-36
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• 2020

[Purpose] The purpose of this study was to investigate the effects of short-term creatine intake on muscle fatigue induced by resistance exercise in healthy adolescent men, i.e., lactic acid concentration and wrist and head tremor measured by an accelerometer. [Methods] Twelve healthy adolescent men who had no experience with creatine intake were included. The subjects were randomly assigned to the creatine group and the placebo group, followed by 5 days of creatine and placebo intake, and 5 times of 5 sets of leg press, leg extension, bench press, and arm curl exercises at 70% repetition maximum (RM). The lactic acid concentration before and after exercising, rate of perceived exertion (RPE), and accelerometer-based wrist tremor and head tremor during exercise were measured. Subsequently, after 7 days to allow for creatine washout, the same exercise treatment and measurement were performed in each group after switching drug and placebo between the groups. [Results] The level of lactic acid before and after the acute resistance exercise trial was significantly lower in the creatine group than in the placebo group (P <0.05). The mean RPE during the resistance exercise was significantly lower in the creatine group than in the placebo group (P <0.05). There was no difference between the two groups in the mean wrist tremor during resistance exercise, but the mean head tremor values were significantly lower in the creatine group than in the placebo group in the arm curl, the last event of the exercise trials (P <0.05). [Conclusion] Short-term creatine intake reduces the blood fatigue factor increased by resistance exercise, and is thought to suppress fatigue, especially in the latter half of resistance exercise. Therefore, these findings indicate that short-term creatine intake can have an improved effect on anaerobic exercise performance.

• Annals of Clinical Neurophysiology
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• v.26 no.1
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• pp.30-33
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• 2024

Rippling muscle disease (RMD) is caused by dominant mutations of the caveolin-3 gene (CAV3), and presents with overlapping limb-girdle muscle weakness, elevated creatine kinase (hyper-CKemia), RMD, and distal myopathy. We report a patient with a CAV3 mutation who presented with myalgia, exercise-induced muscle stiffness, hyperCKemia, and percussion-induced rapid muscle contraction and muscle mounding. A familial genetic study revealed the same mutation in two family members, with physical examinations showing that both of them had rippling muscles.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.2
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• pp.537-545
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• 2015

The purpose of this study is to investigate how effects creatine dosage has on the improvement of rowing athletes' performance ability. Rowing athletes were administered with creatine, through which to examine the change of athletic performance ability, blood fatigue substances, and muscular activity. The subjects (participants) of this Study consisted of 12 male rowing athletes at P University, with at least 5 years of rowing experiences, which divided into two groups - creatine dosing group of 6 persons and control group of 6 persons - for random sampling measurement. Enzymatic-colorimetric method using lacrate oxidase and 4-aminoantipyrine was performed for blood lactate level analysis, and wireless EMG system (QEMG-4: Lxtha Korea) for muscular activity analysis, with 4 channels set for data analysis. As body parts to be measured, two muscular parts - latissimus dorsi and lumbar spinel - were chosen. Then, on the 5th day from the date of administering them with creatine (that is, 4 days after dosing them with creatine), rowing movement with the highest level of activity was calculated as peak value, which was measured twice. The test data used for this Study were SPSS/PC 18.0, pre-movement and post-movement two-way ANOVA for repeated measurement for comparative analysis of each muscle, with significant level at .05. As a result, the change of blood lactate level was significantly higher in creatine dosing group than in non-dosing group (p<.05). As for the change of muscular activity, both latissimus dorsi and lumbar spinel showed a significantly higher change of muscle in creatine dosing group than in non-dosing group (p<.05 and p<.05, respectively).

• Toxicological Research
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• v.19 no.2
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• pp.115-121
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• 2003

In view of the importance of muscle-specific creatine kinase (CKMM) gene as a genetic factor for athletic performance, we investigate the relationship between elite athletic performance and two restriction fragment length polymorphisms (Ncol and Taql RFLPs) in the CKMM gene. Genomic DNA was extracted from white blood cells of 98 unrelated male Korean elite athletes and 04 sedentary controls, respectively. Two genetic polymorphisms in the CKMM gene were detected by the polymerase chain reaction and the digestion with restriction endonucleases, Ncol and Taql, respectively. There were no significant associations between two genetic polymorphisms in the CKMM gene and elite athletic performance or clinical parameters in our subjects. Therefore, these findings suggest that two genetic polymorphisms in the CKMM gene may not be useful as genetic markers to predict the athletic performance in male Koreans.

• The Journal of Korean Physical Therapy
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• v.14 no.3
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• pp.74-91
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• 2002

This studies to investigate the effects of ultrasound and swimming on the changes of the serum creatine kinase, latate dehydrogenase, aspatate aminotransferase, alanine aminotransferase, alkaline phosphatase, in peripheral nerve and muscle injury rats. The forty Sprague-Dawley adult male rats were assigned to the 4 groups: the experimental groups(3), and the peripheral nerve and muscle injury control group(1). There was made artificial injured by ischial nerve and muscle of each rats the each experimental ultrasound group and swimming group were treated from 3 days after being injuried for the 5 minutes and 10 minutes every day during the 10 days and 16 days respectively. The results were as follows: 1. The activities of creatine kinase, lactate dehydrogenase were significantly decreased on of the ultrasound and swimming groups for 10 days. All experimental group were significantly decreased for 16 days. there were significantly decreased the swimming group and ultrasound group were to the 10 days group. 2. The activities of asparatate aminotransferase, alalnine aminotransferase on the ultrasound group were significantly increased to the injured control group, there were significantly decreased the ultrasound group for 16 days group were to the 10 days group and there were significantly increased the swimming group for 16 days group were to the 10 days group. 3. The activities of alkaline phosphatase changes were no difference all experimental groups, there were significantly increased for 16 days group to the 10 days group. From these results it may be concluded that the effects of the ultrasound and swimming, the changes of the serum activities creatine kinase , lactate dehydrogenase of (; the objective indicates) to the muscle regeneration process of the nerve and muscle injured rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.9 no.1
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• pp.59-73
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• 1980

A detailed procedure was described for the isolation of cratine kinase (ATP-Creatine phosphotransferase, E. C. 2. 7. 3. 2.) from the muscle of the snake Bungarus fasciatus. The original isolation procedure of Kuby et al. for the rabbit muscle enzyme has been modified and extended to include a chromatographic step. The properties of the enzyme have been investigated and kinetic constants for the reverse reactions determined as the followings: 1) A molecular weight of the enzyme was determined by gel filteration on Sephadex G-100 and by electrophoresis on SDS-polyacrylamide was 86,000. 2) Two reactive sulphydryl groups were detected with dithiobis nitrobenzoic acid (DTNB). 3) The nucleotide substrate specificity in the reverse reaction was determined as ADP*2'-dADP＞GDP＞XDP＞UDP with magnesium as the activating metal ion. 4) The order of the metal specificity in the reverse reaction Mg>Mn>$Ca{\sim}Co$ was determined with ADP as substrate. 5) A detailed kinetic analysis was carried out in the reverse direction with $MaADP^-$ as the nucleotide substrate. Initial velocity and product inhibition studies($MaADP^{2-}$ competitive with respect to MgADP- and noncompetitive with respect to $N-phosphorycreatine^{2-}$ ; Creatine competitive with respect to $N-phosphorycreatine^{2-}$ and noncompetitive with respect to Ma $ADP^-)$ indicated that the reaction obeyed a sequential mechanism of the rapid equilibrium random type.

• Journal of The Korean Society of Integrative Medicine
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• v.2 no.3
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• pp.31-37
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• 2014

Purpose : Delayed onset muscle soreness(DOMS) is a common problem that can interfere with rehabilitation as well as activities of daily living. The purpose of this study was to compare the effect of both trascutaneous electrical nerve stimulation(TENS) Micro current(MC) and only normal Therapy on Delyed Onset Muscle Soreness(DOMS). Method : The Methods ten untrained and male volunteer subjects were randomly assigned to one of two treatment groups: 1) a group that received TENS (60Hz) MC($60{\mu}A$, 3pps) a control group that received no MC treatment. Subjects performed repeated eccentric exercise of the non-dominant forearm flexor muscle with submaximal intensity by the simply designed eccentric exercise devices. Treatments were applied after 24hours and 48hours. Subjects attended on two consecutive days for treatment and measurement of paining(visual analogue scale: VAS) and CK(Creatine kinase) on a daily basis. Measurements were taken after treatment. Results : 1) There were no significant differences between TENS and MENS by two-way repeated ANOVA. The Results that t-test for VAS revealed significant differences within TENS group. 3) The t-test for VAS, and Creatine Kinase of time revealed significant differences within MC group. Conclusion : These findings suggest that both TENS and MENS had effect on DOMS.

• Korean Journal of Veterinary Service
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• v.17 no.2
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• pp.114-121
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• 1994

Clinically healthy 12 female Manina breed chicken (6 of 75 days old : group 46 of 145 days old： group B, female) were examined to establish physiological basic data on organ tissues total Creatine phosphokinase (CPK) activities and CPK isoenzymes fractions. The tissues examined were the Lung, Heart, Liver, Proventriculus, Gizzard, Duodenum, Colon and Muscle. The results obtained were summarized as follows : 1. Total CPK activities were high with decreasing order of the Muscle > Proventriculus >Giz-zard >Heart >Duodenum> Colon >Lung >Liver in group A and Muscle >Proventriculus >Giz-zard >Heart > Colon >Duodenum > Lung > Liver in group B. Significance of total CPK activities in group difference was only found Colon, group B showed higher values than that of group A (p< 0.01). 2. In the pattern of CPK isoenzymes fractions, Lung, Heart, Liver, Proventriculus, Gizzard, Duodenum and Muscle were high with decreasing order of CK2 >CK3, Colon showed the pattern with decreasing order of CK3 >CK2. Significance of CPK isoenzymes fractions in group difference was only found Liver, CK2 in group B(P<0.01) and CK3 in group A(P<0.01) were higher than that of the other group.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1680-1685
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• 2015

Since ancient days, domestic horses have been closely associated with human civilization. Today, horse racing is an important industry. Various genes involved in energy production and muscle contraction are differentially regulated during a race. Among them, creatine kinase (CK) is well known for its regulation of energy preservation in animal cells. CK is an iso-enzyme, encoded by different genes and expressed in skeletal muscle, heart, brain and leucocytes. We confirmed that the expression of CK-M significantly increased in the blood after a 30 minute exercise period, while no considerable change was observed in skeletal muscle. Analysis of various tissues showed an ubiquitous expression of the CK-M gene in the horse; CK-M mRNA expression was predominant in the skeletal muscle and the cardiac muscle compared to other tissues. An evolutionary study by synonymous and non-synonymous single nucleotide polymorphism ratio of CK-M gene revealed a positive selection that was conserved in the horse. More studies are warranted in order to develop the expression of CK-M gene as a biomarker in blood of thoroughbred horses.