• 디지털융복합연구
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• 제15권12호
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• pp.469-475
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• 2017

논고는 복합문화적인 측면에서 바라 본 현대도시와 공공디자인의 특징 등에 대한 것이다. 본 연구는 현대도시와 문화에 대한 연구를 위해 관련 문헌 조사 및 다양한 이론적 고찰 등 실증주의적 연구 방법을 적용하였다. 본 연구의 목적은 문화와 복합문화에 대한 논의와 그에 따라 현대도시와 공공디자인과의 관계성을 모색하기 위한 것이다. 이에 따라 본 연구에서는 우리나라의 대표적 복합문화 공간으로서 스타필드 코엑스 사례를 분석하고 이를 공공디자인의 개념으로 재해석하였다. 덧붙여 현대도시가 가진 문화적 혼종성과 복합성의 성격을 공공공간에 융합하여 공공시설물에서의 사적공간의 필요성을 제언하였다. 하지만 본 연구는 우리나라의 복합문화의 개념 정의에 있어서 이론적 논의는 있었으나 이를 뒷받침 할 수 있는 실증적 사례를 제시하지 못하는 한계점을 가지고 있다. 이는 후속연구인 현대도시의 복합문화공간에 관한 고찰을 통해 실제 사례를 조사 연구를 통해 보완하도록 하겠다.

• Clinical and Experimental Pediatrics
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• 제55권11호
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• pp.424-429
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• 2012

Purpose: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae -positive results. Methods: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Children's Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. Results: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. Conclusion: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2006년도 춘계학술발표대회 논문집
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• pp.158-164
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• 2006

Multiplex have many screen in one place and provide customer Multiple Entertainment Option. Today multiplex cinema is a new culture place for the public to approach most easily in Korea. It also gave a chance to recognize how important a popular culture place is. but it has a problem that this space wasn't designed for customer and don't have even a rest area for them as they focused only on economic growth. therefore, in this study, it point's the utilization of Service Areas problems and provide basic data of interior design for customer.

• Journal of Information Technology Applications and Management
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• 제25권4호
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• pp.123-143
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• 2018

The domestic multiplex industry provides consumers with a choice of movies and a variety of contents and entertainment facilities and services. In addition, the number of movie theaters with the significant market potential is also steadily increasing in the competitive multiplex market environment. For the analysis, we conducted research on 300 adolescents who have experienced using domestic multiplex mobile service within the recent year. This study examined the structural relationship among the multi-dimensional mobile service quality of multiple, enjoyment of use, and online word of mouth intention. Also, it explored the mediating effect of enjoyment of use and the moderating effect of gender in the structural model. As a result, the mobile service quality of multiplex has a significant effect on the online word of mouth intention through the enjoyment of use. However, there was no moderating effect of gender of participating adolescents in the relationships. Based on the analysis of empirical results, this study discussed a series of theocratical and practical implications for the marketing strategies of multiplex in the highly competitive market.

• 대한수의학회지
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• 제39권2호
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• pp.343-352
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• 1999

Vancomycin resistant enterococci (VRE) have emerged as an important nosocomial pathogen. Since 1989 the Center for Disease Control, United States, has reported a rapid increase in the incidence of enterococcal bacteremia and endocarditis infection by VRE. It was suggested that the use of avoparcin was associated with the appearance of VRE in animal husbandry. To date, several detection methods have been used based on conventional methods of culture and gene detection. However, these methods have some limitations such as time-consuming, laborious and additional differential needs. Therefore, In this study a multiplex PCR method was established to detect and differentiate resistance types of enterococci which specifically amplify the four van genes encoding vancomycin resistance elements. Using the method, we investigated the incidence rates and types of VRE from farms using or not using avoparcin. A total of 1091 animal fecal samples were collected from 70 pig and 32 poultry farms. A total of 425 of enterococci were isolated from samples. Of the 425 isolates, 11 of the them showed a pattern of high-level vancomycin resistance (MIC : $64{\sim}256{\mu}g/ml$) which was associated with the presence of the vanA or vanB gene. Fifty-seven isolates showed a pattern of low-level vancomycin resistance (MIC : $3{\sim}8{\mu}g/ml$) associated with the vanC-1 or vanC-2 gene. Interestingly, all isolates with high-level vancomycin resistance were from farms that have never used avoparcin. Moreover, the high-level VRE isolation rate in Korea (2.58%) was much lower than that of other countries (50% in England, 7% in Belgium) where avoparcin have been used. In conclusion, the multiplex PCR method established in this study could be applied for detection of VRE.

• 문화경제연구
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• 제21권1호
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• pp.3-20
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• 2018

본 연구는 한국 영화산업의 수직계열화 구조와 배급 및 상영시장의 집중도를 파악하고, 이 두 범주 간의 상호연관성에 대한 통합적인 맥락을 제시하고자 하였다. 한국영화 배급 및 상영시장의 집중성은 규모의 경제를 추구하고 수요의 불확실성을 방어하기 위한 산업 논리의 결과이다. 메이저 배급사는 대작영화에 자원을 집중하고 공급량을 조절함으로써 흥행위험을 방어하려는 경향이 강해지고 있으며, 최대한 많은 스크린을 확보하려는 배급 경쟁에 의해 멀티플렉스 체인의 협상력은 강화되고 있다. 수직결합 기업의 멀티플렉스는 계열관계에 따라 각 배급사의 영화에 차별적인 좌석수를 배정하는 것으로 나타났다. 그러나 수직계열화 기업마다 차별의 정도는 차이가 있으며, 상영 스크린수가 증가할수록 관객의 좌석점유율도 증가하고 있어서, 상영관이 계열관계에 따라 비합리적인 선택을 한다고 보기에는 무리가 있다. 따라서 수직결합구조가 스크린 독과점과 양극화의 원인이라는 일반화하기는 어려우며 유통시장의 집중성을 완화하고 중소영화를 지원하는 정책 방향이 요구된다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.135-135
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• 2008

Various biosensors were evaluated for identifying and detecting foodborne pathogens in a rapid and effective manner. First, five strains of Escherichia coli and six strains of Salmonella were identified using Fourier transform infrared spectroscopy and a statistical program. For doing this, lipopolysaccharides (LPSs) and outer membrane proteins (OMPs) were extracted from a cell wall of each bacterial strain. As a result, each strain was identifed at the level of 97% for E. coli and 100% for Salmonella. Second, E. coli O157:H7, S. Enteritidis, and Listeria monocytogenes were identified by multiplex PCR products from four specific genes of each bacteria using a capillary electrophoresis (CE). Also, ground beef for E. coli O157:H7, lettuce for S. Enteritidis, and hot dog for L. monocytogenes were used to determine the possibility of detecting pathogens in foods. Foods inoculated with respective pathogen were cultivated for six hours and multiplex PCR products were obtained and assessed. The minimum detection levels of tested bacteria were <10 cells/g, <10 cells/g, and $10^4$ cells/g for E. coli O157:H7, S. Enteritidis, and L. monocytogenes, respectively. Third, it was possible to detect S. Typhimurium in a pure culture and lettuce by a bioluminescence-based detection assay using both recombinant bacteriophage P22::luxI and a bioluminescent bioreporter. In addition, bacteriophage T4 was quantitatively monitored using E. coli including luxCDABE genes.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1481-1489
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• 2021

Early and accurate detection of pathogens is important to improve clinical outcomes of bloodstream infections (BSI), especially in the case of drug-resistant pathogens. In this study, we aimed to develop a culture-independent digital PCR (dPCR) system for multiplex detection of major sepsis-causing gram-negative pathogens and antimicrobial resistance genes using plasma DNA from BSI patients. Our duplex dPCR system successfully detected nine targets (five bacteria-specific targets and four antimicrobial resistance genes) through five reactions within 3 hours. The minimum detection limit was 50 ag of bacterial DNA, suggesting that 1 CFU/ml of bacteria in the blood can be detected. To validate the clinical applicability, cell-free DNA samples from febrile patients were tested with our system and confirmed high consistency with conventional blood culture. This system can support early identification of some drug-resistant gram-negative pathogens, which can help improving treatment outcomes of BSI.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.639-644
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• 2019

Diarrhea is one of the most common infectious diseases known worldwide. However, few studies have examined anaerobic diarrhea-causing bacteria (DB), which are difficult to culture. Recent advances in molecular biology have facilitated the detection and analysis of anaerobic DB. In this study, long-term trends in anaerobic DB were evaluated in Korea. From 2010 to 2018, symptoms of diarrhea reported were analyzed among patients hospitalized at the Dankook University Hospital in Korea. Results of multiplex polymerase chain reaction based on seasonality, age, overlapping infection, and other factors in patients were evaluated. DB were detected in 38.2% of 1716 stool specimens in the duration of the study. Of the pathogens detected using this method, 49.8% (n = 405/813) were anaerobic bacteria, including Clostridioides perfringens, Campylobacter spp., Clostridioides difficile toxin B, and Aeromonas spp. Among the four anaerobic bacteria, Clostridioides perfringens was the most commonly occurring (15.5%; n = 126/813). Detection rates of Clostridioides perfringens, Clostridioides difficile toxin B, and Aeromonas spp. were 34.1% (n = 22/55), 34.9% (n = 43/126), and 40.0% (n = 38/109), respectively. The detection rate of Campylobacter spp. (32.7%; n = 37/115) was the highest in patients between 10 and 20 years of age. The detection rate of anaerobic DB showed an increase in 2018 as compared with that in 2010, and the number of events of diarrhea caused by anaerobic DB also increased in this duration. Further studies are required to devise methods that might prevent the proliferation of anaerobic DB.

• Gut and Liver
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• 제12권6호
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• pp.648-654
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• 2018

Background/Aims: Dual priming oligonucleotide-based multiplex polymerase chain reaction (DPO-based PCR) can detect the presence of clarithromycin resistance without culture. The aim of this study was to investigate the cost-effectiveness of DPO-based PCR for Helicobacter pylori eradication. Methods: From 2015 to 2016, medical records of patients who received H. pylori eradication therapy were analyzed. Patients were divided into two groups: tailored group patients who were treated based on DPO-based PCR and empirical group patients. Eradication rate and medical cost, including diagnostic tests, eradication regimens, and $^{13}C$-urea breath tests, were compared between the two groups. Cost for one successful eradication was calculated in each group. The expected cost of eradication for empirical treatment was investigated by varying the treatment duration and eradication rate. Results: A total of 527 patients were analyzed (tailored group 208, empirical group 319). The eradication success rate of the first-line therapy was higher in the tailored group compared to that in the empirical group (91.8% vs 72.1%, p<0.01). The total medical cost for each group was $114.8{\pm}14.1U.S.$ dollars (USD) and $85.8{\pm}24.4USD$, respectively (p<0.01). The total medical costs for each ultimately successful eradication in the tailored group and in the empirical group were 120.0 USD and 92.4 USD, respectively. The economic modeling expected cost of a successful eradication after a 7- or 14-day empirical treatment was 93.8 to 111.4 USD and 126.3 to 149.9 USD, respectively. Conclusions: Based on economic modeling, the cost for a successful eradication using DPO-based PCR would be similar or superior to the expected cost of a successful eradication with a 14-day empirical treatment when the first-line eradication rate is ${\leq}80%$.